Investigation of the lipid peroxidation, antioxidant enzymes, antioxidant vitamins, oxidation products of nitric oxide and some biochemical parameters in chicken with infectious bursal disease (IBD)

Kidney stones are known to haunt humanity for centuries and increase in oxalate is a predominant risk factor for stone formation. The present study was initiated with a notion to study the oxidative and nitrosative stress on erythrocytes under oxalate stress and the putative role of sulphated polysaccharides. Hyperoxaluria was induced in two groups by the administration of 0.75% ethylene glycol in drinking water for 28 days and one of them was treated with sulphated polysaccharides from Fucus vesiculosus from the 8th day to the end of the experimental period of 28 days at a dose of 5 mg/kg body weight subcutaneously. Control and drug control (sulphated polysaccharides alone) were also included in the study. Glycolic and glyoxylic acid levels of urine were analyzed as an index of hyperoxaluria. The plasma enzymic markers of cellular integrity, redox status of red blood cells, osmotic fragility, and 14C-oxalate binding were investigated. Urine and plasma nitric oxide metabolites, expression of inducible nitric oxide synthase protein, and mRNA were assessed in kidney to evaluate the nitrosative stress. Increased levels of glycolic and glyoxylic acid in urine indicated the prevalence of hyperoxaluria in ethylene glycol–administered groups. Plasma aspartate and alanine transaminase were not altered, but alkaline phosphatase and lactate dehydrogenase of hyperoxaluric group were increased indicating tissue damage. Activities of antioxidant enzymes were decreased, whereas erythrocyte membrane lipid peroxidation was increased in hyperoxaluric rats. Moreover, an altered fragility with an increase in oxalate binding activity was observed in hyperoxaluric group. Increase in nitric oxide metabolites levels in urine and plasma along with an increase in expression of inducible nitric oxide synthase protein and mRNA in kidney were observed in hyperoxaluric rats. Administration of sulphated polysaccharides to hyperoxaluric rats averted the abnormal increase in urinary glycolic and glyoxylic acid levels and enzyme activities, decreased lipid peroxidation, and increased the activities of antioxidant enzymes. Furthermore, increased nitrosative stress accompanying hyperoxaluria was also normalized on sulphated polysaccharides treatment. To conclude, sulphated polysaccharide administration was able to maintain the integrity of erythrocyte membrane and decrease the damage to erythrocytes in hyperoxaluria.

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The effect of N-stearoylethanolamine on the activity of antioxidant enzymes, content of lipid peroxidation products and nitric oxide in the blood plasma and liver of rats with induced insulin-resistance

The Ukrainian Biochemical Journal ◽

10.15407/ubj85.05.088 ◽

2013 ◽

Vol 85 (6) ◽

pp. 88-96 ◽

Cited By ~ 1

Author(s):

O. V. Onopchenko ◽

Keyword(s):

Nitric Oxide ◽

Insulin Resistance ◽

Lipid Peroxidation ◽

Antioxidant Enzymes ◽

Blood Plasma ◽

Lipid Peroxidation Products

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Role of nitric oxide in improving seed germination and alleviation of copper-induced photosynthetic inhibition in Indian mustard

10.1101/865162 ◽

2019 ◽

Author(s):

Bilal A. Rather ◽

Iqbal R. Mir ◽

Asim Masood ◽

Naser A. Anjum ◽

Nafees A. Khan

Keyword(s):

Nitric Oxide ◽

Lipid Peroxidation ◽

Heavy Metal ◽

Antioxidant Enzymes ◽

Seed Germination ◽

Crop Production ◽

Germination Rate ◽

Heavy Metal Stress ◽

Metal Stress ◽

Mustard Plant

AbstractHeavy metal stress limits crop production through its effects on seed germination and photosynthesis. Nitric oxide (NO), a versatile signaling molecule, plays a significant role in heavy metal stress tolerance. In the present investigation, the efficacy of NO application in the alleviation of copper (Cu) induced adverse impact on seed germination and photosynthesis of mustard plant (Brassica juncea L.) was evaluated. Pretreatment with NO donor sodium nitroprusside (SNP), significantly improved seed germination and alleviated Cu-accrued oxidative stress in B. juncea seeds. However, in the absence of NO, Cu showed a higher reduction in seed germination rate. Further, NO modulated the activities of antioxidant enzymes and sustained the lower level of lipid peroxidation by reducing H2O2, and thiobarbituric acid reactive substances (TBARS), thereby elevated the antioxidative capacity in Cu-exposed seeds. Seeds pretreated with NO also retained higher amylase activities for the proper seed germination when compared with control. NO mitigated Cu toxicity through an improved antioxidant system, and reducing Cu-induced accumulation of reactive oxygen species (ROS), reduction in lipid peroxidation improving photosynthetic efficiency and growth of the mustard plant. It may concluded that NO improved amylase activity, modulated activity of antioxidant enzymes, and enhanced the germination rate seeds under Cu stress, thereby improved photosynthesis and growth.

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THE STUDY OF FREE RADICALS TO EVALUATE THE HEALING EFFECTS OF PUNICA GRANATUM PEEL ON ACETIC ACID-INDUCED COLITIS IN RATS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2022.v15i1.43894 ◽

2021 ◽

pp. 66-68

Author(s):

ARPIT VERMA ◽

MANI GOEL ◽

SATYENDRA PRAJAPATI ◽

ANAND BHADKARIYA

Keyword(s):

Nitric Oxide ◽

Lipid Peroxidation ◽

Acetic Acid ◽

Free Radicals ◽

Biochemical Parameters ◽

Ethanolic Extract ◽

Punica Granatum ◽

Mucosal Damage ◽

Oral Route ◽

Effective Administration

Objective: The objective of the study was to analyze the effect of 50% ethanolic extract of the dried Punica granatum peel (PGE) on the healing of acetic acid-induced colitis in rats. Methods: Colitis was induced in rats using 50% acetic acid and then PGE extract was administered by oral route daily for 14 days to those rats. Optimal healing was observed by the administration of a 100 mg/kg dose of PGE extract. Effectiveness of the above-mentioned dosage of PGE on biochemical parameters, basically free radicals – nitric oxide and lipid peroxidation was studied on 18 hours fasting rats on the 15th day of the experiment. Results: The results were suggestive of the healing properties of PGE extract by reduction of the inflammation and mucosal damage in the colon of those rats. The healing effects were established by the estimation and study of free radicals taken from the mucosal samples of the rat’s colon. The safety of extract was established by the effective administration of 10 times the therapeutic dose, that is, 1000 mg/kg dosage of the PGE extract with no noticeable adverse effects or side effects related to ANS or central nervous system. Conclusion: PGE extract was found to be effective in healing mucosal damage due to colitis by controlling the infection and reducing the inflammation.

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