scholarly journals Enzyme activities in soil at increasing metal (Cu, Ni, Pb) doses and time-dependence in a model experiment

2020 ◽  
Vol 14 (1) ◽  
pp. 43-49
Author(s):  
Klára Czakó-Vér ◽  
Dávid Somfai ◽  
Erzsébet Suhajda ◽  
Csilla Sipeky ◽  
Gyula Árvay ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Enzyme Activities ◽  
Model Experiment ◽  
Soil Microbial Activity ◽  
Water Soluble ◽  
Metal Salts ◽  
Laboratory Model ◽  
Soil Microbial ◽  
Set Up ◽  
Metal Addition

Aim of this paper is to examine the effect of spiked copper (Cu), nickel (Ni) and lead (Pb) metal salts on the dehydrogenase (oxydo-reductase) and phosphatase (hydrolase) enzyme activities in a characteristic Hungarian soil, the pseudomycelliar chernozem. Pot-experiment was performed with a soil, originating from a spot of the Hungarian soil-information-monitoring (TIM) system of Bicserd. The added metal salts were used in water soluble forms and incorporated uniformly to the soil. Soils were treated with increasing metal concentrations to give the following metal amounts: 0, 50, 200, and 800 kg.ha-1. Enzyme activities of the soil were analysed at the 0th, 7 th, 14 th, and 28th days after the metal addition. The laboratory model-experiment has been set up in three replicates. Effects of metal salts were largely dependent on the chemical and physical properties of pseudomycelliar chernozem soil, the applied heavy metal-types, the doses of used metals and the elapsed time after the pollution. Considering the different metals, the copper prowed to be the most toxic one on the studied enzyme activities, whereas the lead induced those. By comparison with copper the nickel affected a smaller decrease in the soil microbial activity. The dehydrogenase, oxydo-reductase enzyme was found to be more sensitive parameter in comparison with the phosphatase, hydrolase enzyme among the studied condition. Studied enzymes and used methods are suggested, as fast and rather reliable tools for estimating the soil-resilience capacities at heavy metal pollution.

The Application of Red Mud on Restoring Heavy Metal Contaminated Water and Soil

2011 ◽  
Vol 225-226 ◽  
pp. 1262-1265 ◽  
Author(s):  
Yan Fei Ma ◽  
Xue Dong Feng
Keyword(s):  
Heavy Metal ◽  
Metal Ions ◽  
Red Mud ◽  
Heavy Metal Ions ◽  
Alkali Treatment ◽  
Low Cost ◽  
Soil Microbial Activity ◽  
Contaminated Water ◽  
Environmental Restoration ◽  
Soil Microbial

Red mud as environmental restoration materials has the characteristics of low cost, simple process and controlling waste by waste. The paper introduced the application of red mud on heavy metal contaminated water and soil in details. Powdered or granular red mud both has good adsorption efficiency of Cu , Zn, Pb, Ni, Ca, Cd, As and other heavy metal ions in wastewater. Langmiur or Frendlich model can express the adsorption isotherm of red mud on heavy metal ions. Red mud can not only solidify the heavy metals in soils, but also increase the soil microbial activity and population types. At the same time, de-alkali treatment can not be ignored in the process of using red mud avoiding the second pollution to environment, and achieve the zero pollution release of red mud.

scholarly journals Responses of Soil Microbial Activity to Swine Manure Applications

2020 ◽  
Vol 12 (9) ◽  
pp. 199
Author(s):  
Maria Josiane Martins ◽  
Tânia Santos Silva ◽  
Igor Paranhos Caldas ◽  
Geovane Teixeira de Azevedo ◽  
Isabelle Carolyne Cardoso ◽  
...  
Keyword(s):  
Microbial Biomass ◽  
Microbial Activity ◽  
Microbial Respiration ◽  
Swine Manure ◽  
Soil Microbial Activity ◽  
Soil Microbial ◽  
Liquid Swine Manure ◽  
Randomized Design ◽  
Set Up ◽  
The Impact

The allocation of the large amount of swine waste from farms is an international concern. An efficient way of managing such waste is its use in farming. It is already known that the incorporation of organic waste into the soil significantly increases the microbial population. Therefore, the objective was to evaluate the impact of the use of swine manure on the soil microbiota in a Eutrophic Oxisol. The experiment was set up in a completely randomized design in a 6 × 4 factorial scheme (sixconcentrations of swine manure and four evaluation periods) with four replications. We evaluate the following characteristics: microbial respiration (C-CO2), microbial biomass (µC g-1 soil) and pH.: microbial respiration (C-CO2), microbial biomass (µC g-1 soil) and pH. A significant effect was found in the interaction between concentrations and time of incubation (p < 0.05) of swine manure on microbial activity in the soil. The amount of microbial carbon increased as a function of increased levels of liquid swine manure. No interaction was observed between concentrations and time of incubation for the pH. The evaluation of the isolated factors allowed to observe that the pH decreased as the doses of manure were incremented. Higher and lower pH values were found after 5 and 30 days of incubation. The application of liquid swine manure up to 6000 L ha-1 increases the release of CO2 and carbon in the microbial biomass. The applications of liquid swine manure cause a gradual reduction in soil pH.

scholarly journals Soil microbial activity under conventional and organic production of bean and maize

2016 ◽  
pp. 35-43
Author(s):  
Jelena Marinkovic ◽  
Ivan Susnica ◽  
Dragana Bjelic ◽  
Branislava Tintor ◽  
Mirjana Vasic
Keyword(s):  
Microbial Activity ◽  
Farming System ◽  
Soil Microbial Activity ◽  
Organic Production ◽  
Soil Microbial ◽  
Number Of Microorganisms ◽  
Microbiological Parameters ◽  
Set Up ◽  
Organic Farming System ◽  
Micro Organisms

The objective of this study was to compare the effects of conventional and organic production system on microbial activity in the soil cultivated with bean and maize crops. The trial in Djurdjevo was set up according to the conventional farming system, while organic farming system was used in Futog. Two maize hybrids and two bean cultivars were used in the trial. Soil samples were collected in two periods during 2014 (before sowing, at flowering stage of bean crops, and at 9-11 leaf stage of maize) at two depths, at both locations. The following microbiological parameters were tested: the total number of micro?organisms, number of ammonifiers, Azotobacter sp., free nitrogen fixing bacteria, fungi, actinomycetes, and activity of dehydrogenase enzyme. The results showed that the total number of microorganisms, number of free N-fixers and dehydrogenase activity were higher within organic production, while Azotobacter sp. was more abundant in conventional production. Variations in the number of ammonifiers, fungi and actinomycetes in relation to the type of production were not obtained. Significant differences in microbial activity were also obtained between period and depths of sampling.

Enhancing Cleanup of Heavy Metal Polluted Landfill Soils and Improving Soil Microbial Activity Using Green Technology with Ferrous Sulfate

2016 ◽  
Vol 6 (1) ◽  
pp. 97-103 ◽  
Author(s):  
Gilbert C. Sigua ◽  
Arnel Celestino ◽  
Ronaldo T. Alberto ◽  
Annie Melinda Paz-Alberto ◽  
Kenneth C. Stone
Keyword(s):  
Heavy Metal ◽  
Microbial Activity ◽  
Ferrous Sulfate ◽  
Soil Microbial Activity ◽  
Green Technology ◽  
Soil Microbial

scholarly journals ELECTRON MICROSCOPY OF DNA MOLECULES "STAINED" WITH HEAVY METAL SALTS

1961 ◽  
Vol 11 (2) ◽  
pp. 297-310 ◽  
Author(s):  
Walther Stoeckenius
Keyword(s):  
Electron Microscopy ◽  
Heavy Metal ◽  
Uranyl Acetate ◽  
Water Soluble ◽  
Metal Salts ◽  
Dna Molecules ◽  
Soluble Salts ◽  
Single Dna Molecules ◽  
Heavy Metal Salts ◽  
Dna Complex

Single DNA molecules can be rendered visible in the electron microscope by "staining" with water-soluble salts of heavy metals. The best results were obtained with lanthanum nitrate, uranyl acetate, and lead perchlorate. The molecules appear as filaments approximately 20 A wide. Their length was not determined, but it could be shown that it varied with the molecular weight of the DNA used. The same heavy metal salts will preferentially "stain" the nucleic acid in a protein-DNA complex. Evidence is provided for the possibility of a partial separation of a double-stranded molecule into single strands on adsorption to the supporting film.

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