scholarly journals ANTIBACTERIAL ACTIVITY OF HYSSOPUS OFFICINALIS ESSENTIAL OILS

2019 ◽  
pp. 191-199 ◽  
Author(s):  
Наталья (Natal'ya) Александровна (Aleksandrovna) Коваленко (Kovalenko) ◽  
Татьяна (Tat'yana) Игоревна (Igorevna) Ахрамович (Ahramovich) ◽  
Галина (Galina) Николаевна (Nikolaevna) Супиченко (Supichenko) ◽  
Татьяна (Tat'yana) Владимировна (Vladimirovna) Сачивко (Sachivko) ◽  
Виктор (Viktor) Николаевич (Nikolaevich) Босак (Bosak)

The steam distillation method was used to obtain samples of essential oil of plants of three varieties Hyssopus officinalis L., cultivated in the conditions of the Republic of Belarus. More than 20 components were identified and determined in the essential oils of plants of the varieties Lazurit, Rozotsvetkovy, Zaveya by gas-liquid chromatography. It has been established that the studied varieties of Hyssopus officinalis belong to the pinocamphone chemotype. The main components are pinocamphone (67.6–76.0%), β-pinene, eugenol, limonene, camphene, sabinene, linalool, 1,8-cineole, α-terpineol, α-pinene, γ-terpinene and pinocampheol. The distribution of the enantiomers of a-and b-pinenes, camphene, limonene and linalool was established in the studied samples. Antimicrobial activity of essential oils and optical isomers of pure β-pinene was investigated on Staphylococcus aureus, Salmonella alony, Bacillus subtilis, Clostridium sp., Escherichia coli Hfr H, Pseudomonas aeruosa by disc method. It is shown that (+)-β-pinene showed higher antibacterial activity to (-)-β-pinene. The increased content of (+)-β-pinene in the varieties of Hyssopus officinalis with pink (Rosacea variety) and white (Zaveya variety) aureoles of flowers caused a higher antibacterial activity of their essential oils against Gram-negative and Gram-positive bacteria.

2017 ◽  
pp. 63-70 ◽  
Author(s):  
Наталья (Natal'ja) Александровна (Aleksandrovna) Коваленко (Kovalenko) ◽  
Галина (Galina) Николаевна (Nikolaevna) Супиченко (Supichenko) ◽  
Татьяна (Tat'jana) Игоревна (Igorevna) Ахрамович (Ahramovich) ◽  
Анна (Anna) Геннадьевна (Gennad'evna) Шутова (Shutova) ◽  
Виктор (Viktor) Николаевич (Nikolaevich) Леонтьев (Leontiev)

The essential oil obtained by hydrodistillation method from plants of three varieties Agastache aurantiaca, cultivated on the Republic of Belarus were investigated. Some morphobiometric parameters of plants 'Tango', 'Apricot Sprite' and 'Fragrant Delight' and oil yields were determined. Using the technique of gas-liquid chromatography essential oil components were identified and determined. The main components of the essential oil from 'Tango' and 'Fragrant Delight' plants were menton (~ 53 and ~ 65 v/v % respectively) and pulegon (~ 36 and ~ 25 v/v % respectively). The samples of 'Apricot' Sprite' essential oil were rich in isomentone (~ 46 v/v %) and pulegon (~ 41 v/v %). All tested essential oils contained (+)-menton and (+)-pulegon enantiomeric forms only. The antibacterial activity of dimethylsulfoxide and ethanolic essential oil solutions against Staphylococcus aureus, Salmonella alony, Bacillus subtilis, Clostridium sp., Escherichia coli Hfr H, Pseudomonas aeruginosa was proved. Ethanol solutions of essential oils at the concentrations 0.001–0.1 v/v % had significant bactericidal activity. A correlation between the composition of the essential oil and their antibacterial properties was established.


Author(s):  
N. A. Kovalenko ◽  
G. N. Supichenko ◽  
V. N. Leontiev ◽  
A. G. Shutova

The steam distillation method was used to obtain samples of essential oil of some species of genus Agastache L. introduced in Belarus. More than 20 components have been identified and determined in the essential oils of Agastache pallidiflora ssp.neomexicana L., Agastache aurantiaca L., Agastache mexicana L., Agastache foeniculum L. by gas liquid chromatography.The quantitative composition of the samples of essential oils depends on the type of plant material. The main its components are limonene, 1.8-cineol, mentone, isomentone, methyl chavicol, methyeugenol. The main component of essential oils Agastache pallidiflora ssp. neomexicana L. и Agastache aurantiaca L is mentone (40–65 %). Essential oil Agastache foeniculum L. is enriched with pulegone and isomentone. Methyl chavicol and methyleugenol dominate in Agastache mexicana essential oil. Distribution of the enantiomers of limonene, menthone and pulegone are shown. Regardless of the plant species, the samples are optically pure in the (+)-pulegone. The nature of the distribution of the enantiomers of limonene and menthone is an individual characteristic of the studied Agastache L. essential oils.


2019 ◽  
Vol 48 (1) ◽  
pp. 164-171 ◽  
Author(s):  
Татьяна Сачивко ◽  
Tatsiana Sachyuka ◽  
Наталья Коваленко ◽  
Nataliya Kovalenko ◽  
Галина Супиченко ◽  
...  

Basil Ocimum L. belongs to the perspective aromatic and essential-oil plants. Its green mass and essential oils can be successfully used in different sectors of economy including food industry. In 56 samples of basil Ocimum L. having different ecological and geographic origin the author determined the content and yield of essential oils. The study of the taken basil cultivars shows that the content of essential oils in different types of basil (sweet basil – Ocimum basilicum L., holy basil – Ocimum tenuiflorum L., camphor basil – Ocimum kilimandscharicum Willd., hoary basil – Ocimum canum Sims.) was 0.28–0.83% when its yield was 24.0–314.6 kg/ha and green mass yield during technological maturity phase (massive flowering) was 0.51–5.08 kg/m2. By means of gas liquid chromatography method the author performed compositional and anantiometric analysis of the composition of the essential oils obtained from the new basil varieties such as Ocimum L. (Magiya, Volodar, Nastena, Istochnik), bred in Belorussian State Agricultural Academy and catalogued in the National Register of Cultivars in the Republic of Belarus. As a result of the research the author determined that essential oil samples obtained from the new basil cultivars have up to 20 components. The main components are linalool, methyl chavicol, - and -pinenes, -terpineol, eugenol, geraniol, geranial, geranyl acetate, thymol, neral, carvone, carvacrol, limonene and 1.8-cineol. Enantiomers are present in essential oil components - and -pinenes, limonene and linalool.


2018 ◽  
pp. 99-104
Author(s):  
Adelya Sametovna Baimukhambetova ◽  
Lyudmila Timofeyevna Sukhenko ◽  
Anatoliy Valeriyevich Velikorodov ◽  
Mikhail Alekseyevich Egorov ◽  
Gabriele Capodaglio

Plants of Helichrysum genus belong to Asteraceae family and abound with species. The’re two species of plants of Helichrysum genus in the Astrakhan region – Helichrysum arenarium Moench and Hеlichrisum nogaicum Zvelev that was discovered later and isn’t studied well enough yet. Helichrysum arenarium is a well-known medical plant widely used in both academic and traditional medicine and included into the State Pharmacopoeia. Inflorescences of Helichrysum arenarium produce cholagogic, hemostatic, detoxicating, laxative, anti-inflammatory and diuretic effects, which is due to content of flavonoids, terpenoids and other components. This works shows results of studies of chemical composition of extracted essential oils of Hеlichrisum nogaicum and Helichrysum arenarium growing in the Astrakhan Region. Samples of essential oils of plants under studies have been obtained by steam distillation. Optimal steam distillation process duration has been determined by experiment taking studies of dynamics of essential oil yield change through time as a ground. Quantitative analysis of main components of Helichrysum arenarium and Hеlichrisum nogaicum essential oils has been carried out by gas-liquid chromatography. It has been identified that Helichrysum arenarium essential oil contains 21 substances. Its main components are camphor (14.59%) and carboxylic acids (37.02%), and 1,8-cineole (5.97%). The main components of the oil Helichrisum nogaicum are camphor (38.33%), borneol (14.21%), cineol-1.8 (11.37%) and terpene-4-ol (5.18%), sesquiterpene compounds (6.28%), and the content of carboxylic acids is insignificant and amounts to 6.58%.


BioResources ◽  
2007 ◽  
Vol 2 (2) ◽  
pp. 265-269 ◽  
Author(s):  
M. Hakki Alma ◽  
Murat Ertaş ◽  
Siegfrie Nitz ◽  
Hubert Kollmannsberger

In this study, clove bud oil, which was cultivated in the Mediterranean region of Turkey, was provided from a private essential oil company in Turkey. Essential oil from clove (Syzygium aromaticum L.) was obtained from steam-distillation method, and its chemical composition was analyzed by GC and GC-MS. The results showed that the essential oils mainly contained about 87.00% eugenol, 8.01% eugenyl acetate and 3.56% β-Caryophyllene. The chemical composition of the Turkish clove bud oil was comparable to those of trees naturally grown in their native regions.


2011 ◽  
Vol 6 (1) ◽  
pp. 1934578X1100600 ◽  
Author(s):  
Shiva Asbaghian ◽  
Ali Shafaghat ◽  
Khalil Zarea ◽  
Fakhraddin Kasimov ◽  
Farshid Salimi

A comparison of the chemical composition, and antioxidant and antibacterial activities of the essential oils obtained from the aerial parts of Thymus caucasicus, T. kotschyanus, and T. vulgaris was carried out. The oils, obtained by hydrodistillation, were analyzed by GC and GC/MS. Twenty, 29 and 22 compounds representing 94.8%, 96.6% and 98.2% of the essential oils of T. caucasicus, T. kotschyanus and T. vulgaris, respectively, have been identified. The oil of T. caucasicus was characterized by 1,8-cineol (21.5%), thymol (12.6%), β-fenchyl alcohol (8.7%), nerolidol (7.8%), terpinolene (7.2%), α-pinene (7.0%) and myrcene (6.8%). In the oil of T. kotschyanus, carvacrol (24.4%), β-caryophyllene (14.5%), γ-terpinene (12.4%), α-phellandrene (10.8%), p-cymene (9.8%) and thymol (6.8%) were the predominant compounds, whereas the main components of T. vulgaris oil were thymol (43.8%), p-cymene (15.2%), germacrene-D (11.7%), terpinolene (3.4%), carvacrol (3.2%), β-caryophyllene (2.8%) and α-thujene (2.2%). In all three plants oil, hydrocarbon monoterpenes predominated over sesquiterpenes. Antioxidant activities were assessed by determining IC50 values in the DPPH radical scavenging assay. Antibacterial activity was determined by measuring minimum inhibitory concentration (MIC) using the broth dilution method. The essential oils of T. caucasicus, T. kotschyanus and T. vulgaris showed free radical scavenging and antibacterial activity.


2009 ◽  
Vol 4 (6) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Ali Shafaghat ◽  
Hajar Sadeghi ◽  
Khodamali Oji

The water distilled essential oils from leaves, stems and roots of Chrysanthemum parthenium (L.) Bernh. were analyzed by GC and GC/MS methods. The leaf oil was characterized by a high amount of camphor (56.4%), whereas in the stem oil, camphor (26.0%), trans-β-ocimene (23.6%) and germacrene-d (15.0%) were the major constituents. The main components of the root oil were α-pinene (50.0%), trans-β-farnesene (13.8%) and bicyclogermacrene (11.0%). Antibacterial activity of the leaf, stem and root oil were evaluated using the microdilution broth method. The oils showed inhibitory effects on Escherichia coli and Salmonella typhi, but were not active against Staphylococcus aureus.


Holzforschung ◽  
2003 ◽  
Vol 57 (2) ◽  
pp. 140-144 ◽  
Author(s):  
Z. Tunalier ◽  
N. Kirimer ◽  
K.H.C. Başer

Summary Essential oils from heartwood and sapwood of the root and stem of J. foetidissima were analyzed by GC/MS. Cedrol, widdrol, 8,14-cedranoxide, 14-hydroxy-β-caryophyllene, widdrene and α-cedrene were found as main components in the oils. The oil from the root heartwood which was collected as fractions during steam distillation was used for the isolation of constituents. Eight compounds were isolated using chromatographic techniques (such as CC, FC, MPLC, prep HPLC and prep TLC) and characterized by spectral methods as cedrol, widdrol, 8,14-cedrandiol, 8,14-cedranoxide, 14-hydroxy-β-caryophyllene, thujopsenal, α-cedrenal and cadalene.


2020 ◽  
pp. 165-170
Author(s):  
Ra"no Botirovna Karabaeva ◽  
Alidzhan Aminovich Ibragimov ◽  
Otabek Mamadaliyevich Nazarov

The article presents the results of determining the composition of essential oils of Prunus persica var. nectarina varieties "yellow nectarine" – "sariк luchchak (uzb.) ", growing in two regions of the Ferghana region of the Republic of Uzbekistan. The pale yellow essential oil was obtained by hydrodistillation from un-dried fresh leaves of plants. The oil composition was determined by chromatography-mass spectrometry on an Agilent 7890 AGC 6890 N gas chromotograph with a quadrupole mass spectrometer (Agilent 5975C inert MSD) as a detector on an HP-5 MS quartz capillary column. An essential oil of pale yellow color was isolated from the leaves of plants. In the composition of essential oils, 56 and 61 compounds were identified in the first and second samples, respectively, wich is 94.55 and 96.00% of the total components. The dominant components of the first saple are camphor bicyclic monoterpene ketones (24.21%), α-thujone (15.00%) and β-thujone (4.27%), aromatic aldehyde benzaldehyde (18.83%) and isobornoleol bicyclic monoterpene alcohol (6.17%). In the second sample, bicyclic monoterpenic ketones (camphor) (36.67%), α-thujone (21.81%) and β-thujone (7.06%) and bicyclic monoterpene alcohol isobornoleol (9.4%) predominate and monocyclic unsaturated monoterpene α-terpinene (2.18%). In both samples, (+)-2-bornanone (camphor) is predominant. The studied variety Prunus persica var. nectarina can serve as a raw material for the production of essential oil, the main components of which are camphor and isobornoleol.


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