Antibacterial activity of essential oils and their main components enhanced by atmospheric RF plasma

A comparison of the chemical composition, and antioxidant and antibacterial activities of the essential oils obtained from the aerial parts of Thymus caucasicus, T. kotschyanus, and T. vulgaris was carried out. The oils, obtained by hydrodistillation, were analyzed by GC and GC/MS. Twenty, 29 and 22 compounds representing 94.8%, 96.6% and 98.2% of the essential oils of T. caucasicus, T. kotschyanus and T. vulgaris, respectively, have been identified. The oil of T. caucasicus was characterized by 1,8-cineol (21.5%), thymol (12.6%), β-fenchyl alcohol (8.7%), nerolidol (7.8%), terpinolene (7.2%), α-pinene (7.0%) and myrcene (6.8%). In the oil of T. kotschyanus, carvacrol (24.4%), β-caryophyllene (14.5%), γ-terpinene (12.4%), α-phellandrene (10.8%), p-cymene (9.8%) and thymol (6.8%) were the predominant compounds, whereas the main components of T. vulgaris oil were thymol (43.8%), p-cymene (15.2%), germacrene-D (11.7%), terpinolene (3.4%), carvacrol (3.2%), β-caryophyllene (2.8%) and α-thujene (2.2%). In all three plants oil, hydrocarbon monoterpenes predominated over sesquiterpenes. Antioxidant activities were assessed by determining IC50 values in the DPPH radical scavenging assay. Antibacterial activity was determined by measuring minimum inhibitory concentration (MIC) using the broth dilution method. The essential oils of T. caucasicus, T. kotschyanus and T. vulgaris showed free radical scavenging and antibacterial activity.

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Composition and Antibacterial Activity of Essential Oils from Leaf, Stem and Root of Chrysanthemum parthenium (L.) Bernh. from Iran

Natural Product Communications ◽

10.1177/1934578x0900400624 ◽

2009 ◽

Vol 4 (6) ◽

pp. 1934578X0900400 ◽

Cited By ~ 4

Author(s):

Ali Shafaghat ◽

Hajar Sadeghi ◽

Khodamali Oji

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Essential Oils ◽

Salmonella Typhi ◽

Inhibitory Effects ◽

Germacrene D ◽

Leaf Oil ◽

Main Components ◽

Microdilution Broth

The water distilled essential oils from leaves, stems and roots of Chrysanthemum parthenium (L.) Bernh. were analyzed by GC and GC/MS methods. The leaf oil was characterized by a high amount of camphor (56.4%), whereas in the stem oil, camphor (26.0%), trans-β-ocimene (23.6%) and germacrene-d (15.0%) were the major constituents. The main components of the root oil were α-pinene (50.0%), trans-β-farnesene (13.8%) and bicyclogermacrene (11.0%). Antibacterial activity of the leaf, stem and root oil were evaluated using the microdilution broth method. The oils showed inhibitory effects on Escherichia coli and Salmonella typhi, but were not active against Staphylococcus aureus.

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Chemical composition and antibacterial activity of essential oils of Thymelaea hirsuta from Algeria

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d201012 ◽

2019 ◽

Vol 20 (10) ◽

Author(s):

Souheila Bounab ◽

Takia Lograda ◽

Messaoud Ramdani ◽

Pierre Chalard ◽

Gilles Figueredo

Keyword(s):

Antibacterial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Essential Oils ◽

Diffusion Method ◽

Nonanoic Acid ◽

Upgma Cluster Analysis ◽

Oil Concentration ◽

Thymelaea Hirsuta ◽

Main Components

Abstract. Souhila B, Takia L, Messaoud R, Pierre C, Gilles F. 2019. Chemical composition and antibacterial activity of essential oils of Thymelaea hirsuta from Algeria. Biodiversitas 20: 2868-2876. The objectives of this study were to determine the chemical composition and to evaluate the antibacterial activity of Thymelaea hirsuta (L.) Endl., essential oils from seven sampling locations in M'sila region (Algeria). Extraction of essential oils was carried out by the hydro-distillation; the analysis of chemical composition of essential oil was carried out by GC-MS. Antimicrobial activity was performed by disc diffusion method at the essential oil concentration of non-diluted and diluted (1:2, 1:4 and 1:8 v:v of DMSO) against eight species of bacteria. The results showed that the average yields of essential oils were 0.3 ± 0.12%. A total of 45 components were identified, averaging 98.2 ± 1.85% of the total oils. The main components were nonanal-n (10.39 ± 3.21%), hexadecanoic acid (9.77 ± 2.81%), nonanoic acid (9.13 ± 6.49%), triacontane (7.2 ± 3.34%), isopropyl tetradecanoate (6.16 ± 1.99%) and tridecane (4.87 ± 3.1%). Based on the UPGMA cluster analysis, there were two clades of T. hirsuta. T. hirusta has a chemical polymorphism with different chemotypes marked in nature. There were four chemotypes identified in the essential oil of T. hirsuta in the region of M'sila. The essential oil of T. hirsuta has antibacterial activity against eight tested bacteria on the concentration-dependentt manner.

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ANTIBACTERIAL ACTIVITY OF AGASTACHE AURANTIACA ESSENTIAL OILS

chemistry of plant raw material ◽

10.14258/jcprm.2018023317 ◽

2017 ◽

pp. 63-70 ◽

Cited By ~ 2

Author(s):

Наталья (Natal'ja) Александровна (Aleksandrovna) Коваленко (Kovalenko) ◽

Галина (Galina) Николаевна (Nikolaevna) Супиченко (Supichenko) ◽

Татьяна (Tat'jana) Игоревна (Igorevna) Ахрамович (Ahramovich) ◽

Анна (Anna) Геннадьевна (Gennad'evna) Шутова (Shutova) ◽

Виктор (Viktor) Николаевич (Nikolaevich) Леонтьев (Leontiev)

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Essential Oil ◽

Essential Oils ◽

Antibacterial Properties ◽

Gas Liquid Chromatography ◽

Oil Components ◽

The Republic ◽

Main Components

The essential oil obtained by hydrodistillation method from plants of three varieties Agastache aurantiaca, cultivated on the Republic of Belarus were investigated. Some morphobiometric parameters of plants 'Tango', 'Apricot Sprite' and 'Fragrant Delight' and oil yields were determined. Using the technique of gas-liquid chromatography essential oil components were identified and determined. The main components of the essential oil from 'Tango' and 'Fragrant Delight' plants were menton (~ 53 and ~ 65 v/v % respectively) and pulegon (~ 36 and ~ 25 v/v % respectively). The samples of 'Apricot' Sprite' essential oil were rich in isomentone (~ 46 v/v %) and pulegon (~ 41 v/v %). All tested essential oils contained (+)-menton and (+)-pulegon enantiomeric forms only. The antibacterial activity of dimethylsulfoxide and ethanolic essential oil solutions against Staphylococcus aureus, Salmonella alony, Bacillus subtilis, Clostridium sp., Escherichia coli Hfr H, Pseudomonas aeruginosa was proved. Ethanol solutions of essential oils at the concentrations 0.001–0.1 v/v % had significant bactericidal activity. A correlation between the composition of the essential oil and their antibacterial properties was established.

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Antibacterial Activity and Composition of Essential Oils from Flower, Leaf and Stem of Chaerophyllum Macropodum Boiss. from Iran

Natural Product Communications ◽

10.1177/1934578x0900400625 ◽

2009 ◽

Vol 4 (6) ◽

pp. 1934578X0900400 ◽

Cited By ~ 1

Author(s):

Ali Shafaghat

Keyword(s):

Escherichia Coli ◽

Antibacterial Activity ◽

Essential Oils ◽

Antibacterial Activities ◽

Salmonella Typhi ◽

Inhibitory Effects ◽

Leaf Oil ◽

Main Components ◽

Microdilution Broth

The hydrodistilled essential oils from flower, leaf and stem of Chaerophyllum macropodum Boiss. were analyzed by GC and GC/MS. The flower oil was characterized by high amounts of myristicin (42.5%) and trans-β-ocimene (41.0%). In the leaf oil eighteen constituents, representing 99.3% of the total, were identified of which trans-β-ocimene (24.9%), myristicin (15.7%), terpinolene (14.5%), fenchyl acetate (13.9%), cis-β-ocimene (6.3%) and sabinene (6.1%) were major components. The main components of the stem oil were trans-β-ocimene (54.2%), myristicin (22.4%) and sabinene (8.9%). Antibacterial activities of the flower, leaf and stem oils were evaluated using the microdilution broth method. Inhibitory effects on Escherichia coli and Salmonella typhi were recorded.

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The composition and antibacterial activity of essential oils in three Ocimum species growing in Romania

Open Life Sciences ◽

10.2478/s11535-013-0171-8 ◽

2013 ◽

Vol 8 (6) ◽

pp. 600-608 ◽

Cited By ~ 10

Author(s):

Marius Stefan ◽

Maria Zamfirache ◽

Claudia Padurariu ◽

Elena Trută ◽

Irina Gostin

Keyword(s):

Antibacterial Activity ◽

Essential Oils ◽

Glandular Hair ◽

Ocimum Basilicum ◽

Glandular Hairs ◽

E Coli ◽

Hair Morphology ◽

Ocimum Species ◽

Germacrene D ◽

Main Components

AbstractIn this study the glandular hair morphology, chemical composition and antimicrobial activity of the essential oils from three Ocimum species have been investigated (Ocimum basilicum L. var. Genovese, O. gratissimum and O. tenuiflorum). The indumentum shows little variation among the investigated species with both glandular and non-glandular hairs presents. Glandular hairs on the three species are peltate and capitate (with various cell numbers in the stalk and gland). The samples of essential oils obtained from the plant aerial organs by hydrodistillation have been analyzed by GC-MS. Linalool (65.38%, 74.22%, 38.60%), eugenol (5.26%, 3.47%, 10.20%) and tau-cadinol (8.18%, 3.47%, 10.20%) appear as the main components in Ocimum basilicum L. var. Genovese, O. gratissimum and O. tenuiflorum. The oils also contain lower levels of α-bergamotene, 1,8-cineole, germacrene D, β-ocimene, α-caryophyllene, camphor, and α-guaiene. All essential oils showed antibacterial activity against Staphylococcus aureus and Escherichia coli depending on their concentration. Ocimum basilicum L. var. Genovese oil produced the strongest antibacterial effect on S. aureus and E. coli.

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Volatile Oil Constituents of Different Parts of Artemisia Chamaemelifolia and the Composition and Antibacterial Activity of the Aerial Parts of A. Turcomanica from Iran

Natural Product Communications ◽

10.1177/1934578x1200701127 ◽

2012 ◽

Vol 7 (11) ◽

pp. 1934578X1200701 ◽

Cited By ~ 3

Author(s):

Shiva Masoudi ◽

Abdolhossein Rustaiyan ◽

Maryam Vahedi

Keyword(s):

Antibacterial Activity ◽

Essential Oils ◽

Volatile Oil ◽

The Other ◽

Aerial Parts ◽

Significant Difference ◽

Main Components ◽

Main Component ◽

Stem Leaf ◽

Different Parts

The essential oils obtained by hydrodistillation from the aerial parts, stem, leaf and flower of Artemisia chamaemelifolia Vill. and the aerial parts of A. turcomanica Gand. were analyzed by GC and GC/MS. Thirty-one compounds representing 96.6%, 94.6%, 93.2% and 91.0% of the oil were identified in the aerial parts, stem, leaf and flower oils of A. chamaemelifolia. Menthyl acetate (26.5%, 22.0%, 20.5% and 20.5%) and (Z)-nerolidol (20.8%, 26.3%, 14.7% and 18.1%) were the main constituents in the aerial parts, stem, leaf and flower oils, respectively. The other main component in the aerial parts, leaf and flower oils was 1, 8-cineole (13.9%, 11.7% and 12.8%, respectively). Yomogi alcohol (10.4%) and artemisyl acetate (10.4%) were the main components of the leaf and flower oil of the plant, respectively. No significant difference was observed between compositions of the aerial parts, stem, leaf and flower oils. Twenty-four compounds representing 90.5% of the oil of the aerial parts of A. turcomanica were identified, of which 1,8-cineole (15.5%), spathulenol (15.2%), camphor (14.8%), santolina alcohol (14.6%) and trans-β-terpineol (11.6%) were the major ones. The stem, leaf and flower oils of A. turcomanica demonstrated antibacterial activity using the MIC method.

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Chemotypes and antibacterial activity of Matricaria pubescens of Algeria

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d201109 ◽

2019 ◽

Vol 20 (11) ◽

Author(s):

Nour Elhouda Mekhadmi ◽

Messaoud Ramdani ◽

Takia Lograda ◽

Pierre Chalard ◽

Gilles Figueredo

Keyword(s):

Antibacterial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Essential Oils ◽

Diffusion Method ◽

Dependent Manner ◽

Concentration Dependent Manner ◽

Sampling Locations ◽

Upgma Cluster Analysis ◽

Main Components

Abstract. Mekhadmi NE, Ramdani M, Lograda T, Chalard P, Figueredo G. 2019. Chemotypes and antibacterial activity of Matricaria pubescens of Algeria. Biodiversitas 20: 3170-3179. The objectives of this study were to determine the chemical composition and to evaluate the antibacterial activity of Matricaria pubescens L. essential oils from 14 sampling locations in southern Algeria. The extraction of essential oils was carried out by the hydro-distillation; the analysis of chemical composition of essential oil was carried out by GC-MS. Antibacterial activity was performed by disc diffusion method at the undiluted and diluted essential oil (1/1, 1:2, 1:4 and 1:8 v:v of DMSO) against eight species of bacteria (Bacillus cereus, Escherichia coli, Klebsiella pneumoniae, Listeria innocua, Proteus mirabilis, Pseudomonas aeruginosa, Salmonella enterica and Staphylococcus aureus). The results showed that the average yields of essential oils were 0.4 ± 0.27%. A total of 34 components were identified, averaging 99.22 ± 0.8% of the total oils. The main components were β-ocimene (Z) (47.41 ± 13.67%), α-pinene (19 ± 7.36%), β-bulnesene (4.96 ± 10.6%), ocimene-allo (4.46 ± 1.3%), 1-phenyl-penta-2,4-diyne (4.06 ± 5.7%), limonene (3.84 ± 1.8%), caryophyllene oxide (2.36 ± 3.6%) and γ-bisabolene-E (2.08 ± 4.3%). Based on the UPGMA cluster analysis, there were two distinct clades of M. pubescens. This species has a chemical polymorphism with different chemotypes marked in nature. There were four chemotypes identified in the essential oil of M. pubescens in the region studied. The essential oil of M. pubescens has antibacterial activity against eight bacteria tested in a concentration-dependent manner.

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Sub-Tissue Localization of Phytochemicals in Cinnamomum camphora (L.) J. Presl. Growing in Northern Italy

Plants ◽

10.3390/plants10051008 ◽

2021 ◽

Vol 10 (5) ◽

pp. 1008

Author(s):

Martina Bottoni ◽

Fabrizia Milani ◽

Marta Mozzo ◽

Daniele Armando Radice Kolloffel ◽

Alessio Papini ◽

...

Keyword(s):

Antibacterial Activity ◽

Essential Oils ◽

Secretory Cells ◽

Cinnamomum Camphora ◽

Secretory Structures ◽

Plant Parts ◽

Potential Activity ◽

Main Components ◽

First Time

In the present paper, we focused our attention on Cinnamomum camphora (L.) J. Presl. (Lauraceae), studied at three levels: (i) micromorphological, with the analysis of the secretory structures and a novel in-depth histochemical characterization of the secreted compounds; (ii) phytochemical, with the characterization of the essential oils from young stems, fruits, and leaves, subjected to different conservation procedures (fresh, dried, stored at −20 °C, stored at −80 °C) and collected in two different years; (iii) bioactive, consisting of a study of the potential antibacterial activity of the essential oils. The micromorphological investigation proved the presence of secretory cells characterized by a multi-layered wall in the young stems and leaves. They resulted in two different types: mucilage cells producing muco-polysaccharides and oil cells with an exclusive terpene production. The phytochemical investigations showed a predominance of monoterpenes over sesquiterpene derivatives; among them, the main components retrieved in all samples were 1,8-cineole followed by α-terpineol and sabinene. Conservation procedures seem to only influence the amounts of specific components, i.e., 1,8-cineole and α-terpineol, while analyses on each plant part revealed the presence of some peculiar secondary constituents for each of them. Finally, the evaluation of the antibacterial activity of the essential oil showed a promising activity against various microorganisms, as Listeria monocytogenes, Staphylococcus aureus, Enterococcus faecalis and Pseudomonas aeruginosa. In conclusion, we combined a micromorphological and phytochemical approach of the study on different plant parts of C. camphora, linking the occurrence of secretory cells to the production of essential oils. We compared, for the first time, the composition of essential oils derived from different plant matrices conserved with different procedures, allowing us to highlight a relation between the conservation technique and the main components of the profiles. Moreover, the preliminary antibacterial studies evidenced the potential activity of the essential oils against various microorganisms potentially dangerous for plants and humans.

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Antibacterial activity of some essential oils againts Vagococcus salmoninarum

Ege Journal of Fisheries and Aquatic Sciences ◽

10.12714/egejfas.37.2.07 ◽

2020 ◽

Vol 37 (2) ◽

pp. 167-173

Author(s):

Seçil METİN ◽

Zühre İşıl BİÇER

Keyword(s):

Antibacterial Activity ◽

Essential Oils ◽

Nigella Sativa ◽

Zingiber Officinale ◽

Antibacterial Activities ◽

Black Cumin ◽

In Vitro Antibacterial Activity ◽

Main Components

In this study, essential oils of thyme (Origanum vulgare), St. John's Wort (Hypericum perforatum), rosemary (Rosmarinus officinalis), ginger (Zingiber officinale), clove (Eugenia caryophyllata), peppermint (Menta piperita), lavandula (Lavandula hybrida) and black cumin (Nigella sativa) were screened for its chemical composition and in vitro antibacterial activity against Vagococcus salmoninarum. The composition of oils were analysed using GC/MS. Antibacterial effects of essential oils against V. salmoninarum were detected by agar diffusion and tube dilution assays. Thyme (125 μL ml-1 MIC) and clove (500 μL ml-1 MIC) essential oils were shown to possess strong antibacterial activity against V. salmoninarum. The main components of effective essential oils were carvacrol (63.57 %) and eugenol (85.99%), respectively. As a result, these two plant species that tested against V. salmoninarum which is causative agent of vagococcosis can be used as an antibacterial agent. For this purpose, in vivo antibacterial activities against vagococcosis of thyme and clove essential oils should be explored in the further studies.

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