Intrauterine injection of ovine interferon-τ alters oestrogen receptor and oxytocin receptor expression in the endometrium of cyclic ewes

1995 ◽  
Vol 15 (2) ◽  
pp. 203-220 ◽  
Author(s):  
T E Spencer ◽  
N H Ing ◽  
T L Ott ◽  
J S Mayes ◽  
W C Becker ◽  
...  
Keyword(s):  
Progesterone Receptor ◽  
Oestrogen Receptor ◽  
Plasma Concentrations ◽  
Oxytocin Receptor ◽  
Receptor Expression ◽  
Glandular Epithelium ◽  
Receptor Density ◽  
Receptor Mrna ◽  
Prostaglandin F

ABSTRACT This study determined the effects of intrauterine injections of recombinant ovine interferon-τ (roIFN-τ; 2 × 107 antiviral units/day) or control proteins (6 mg/day) from day 11 to day 14 post-oestrus (oestrus=day 0) on endometrial expression of receptors for oestrogen, progesterone and oxytocin in cyclic ewes. Plasma concentrations of progesterone were greater on day 15 in ewes receiving roIFN-τ compared with control proteins (P<0·02, treatment × day). Ewes injected with roIFN-τ had lower endometrial levels of oestrogen receptor mRNA (P<0·10) and protein (P<0·01) on day 15 compared with ewes receiving control proteins. In situ hybridization analysis indicated that oestrogen receptor mRNA was more abundant in the luminal and glandular epithelium of control ewes compared with roIFN-τ-treated ewes. Immunoreactive oestrogen receptor was also present in the luminal and glandular epithelium of control, but not roIFN-τ-treated ewes. Endometrial levels of progesterone receptor mRNA and protein were not different (P>0·10) between control and roIFN-τ-treated ewes. In situ hybridization analyses indicated that progesterone receptor mRNA abundance was low in endometrial epithelium and stroma of both control and roIFN-τ-injected ewes. Immunoreactive progesterone receptors were present in the endometrial stroma and epithelium of control ewes, but confined to the stroma of roIFN-τ-treated ewes. Oxytocin receptor density was lower (P<0·10) in the endometrium of ewes injected with roIFN-τ than control proteins; however, oxytocin receptor affinity was not affected (P>0·10) by treatment. Concentrations of 13,14-dihydro-15-keto-prostaglandin F2α (PGFM) were not increased by exogenous oxytocin administration in control and roIFN-τ-treated ewes on days 10 or 12 post-oestrus. However, on day 14, control ewes responded to oxytocin with increased plasma concentrations of PGFM, whereas ewes receiving roIFN-τ remained unresponsive to oxytocin. These results indicate that the antiluteolytic effects of IFN-τ are to prevent increases in endometrial oestrogen receptor mRNA and protein and oxytocin receptor density which abrogates uterine release of prostaglandin F2α during maternal recognition of pregnancy. IFN-τ may inhibit the synthesis of oestrogen receptor mRNA by a transcriptional or post-transcriptional regulatory mechanism to suppress oxytocin receptor formation during early pregnancy in ewes.

Oxytocin receptor concentrations, inositol phosphate turnover and prostaglandin release by endometrium from ewes induced to ovulate during the early post-partum period

1993 ◽  
Vol 136 (1) ◽  
pp. 17-NP ◽  
Author(s):  
J. M. Wallace ◽  
M. G. Thompson ◽  
R. P. Aitken ◽  
M. A. Cheyne
Keyword(s):  
Receptor Binding ◽  
Inositol Phosphate ◽  
Post Partum ◽  
Oxytocin Receptor ◽  
Receptor Expression ◽  
Luteal Function ◽  
Induction Of Ovulation ◽  
Release Device ◽  
Prostaglandin F

ABSTRACT Induction of ovulation early post partum in sheep is associated with a high incidence (30–40%) of premature luteolysis. The present study was designed to characterize oxytocin receptor levels, oxytocin-stimulated inositol phosphate (IP) turnover (second messenger) and oxytocin-stimulated prostaglandin F2α (PGF2α) release in the endometrium of post-partum ewes induced to ovulate 21 days after parturition and expected to exhibit a range of corpus luteal functions subsequently. Ovulation was induced on day 21 post partum using a controlled internal drug release device and pregnant mare serum gonadotrophin, and uterine tissues were collected on days 5, 10 or 15 of the cycle (n = 4/day). A further 12 ewes whose interval from previous parturition exceeded 150 days were similarly treated and acted as controls. Measurement of daily peripheral progesterone concentrations revealed that while all control ewes exhibited normal luteal function, abnormal luteal function was evident in two, two and one post-partum ewes studied on days 5, 10 and 15 of the cycle respectively. Oxytocin receptor binding was detected (by receptor-binding assay and in-vitro autoradiography) in the endometrium and myometrium of post-partum ewes at all three stages of the oestrous cycle but only at day 15 in control ewes. To determine IP turnover, 100 mg caruncular endometrium was incubated in duplicate for 2·5 h with 10 μCi [3H]inositol and treated with 0 or 2 μmol oxytocin/l for 30 min, then [3H]inositol mono-, bis- and trisphosphates were quantified. Oxytocin stimulated total IPs in all day-5 and day-15 post-partum ewes, in three of four day-10 ewes and in all day-15 control ewes. Basal endometrial PGF2α release measured in triplicate (100 mg/well) during a 2 h incubation was higher in post-partum versus control ewes on days 5 and 10 but not on day 15 of the cycle. Similarly, oxytocin stimulated PGF2α release to varying levels at all stages of the cycle in post-partum ewes but only on day 15 in control ewes. Irrespective of the treatment group endometrial oxytocin receptor number was significantly (P < 0·001) correlated with oxytocin-stimulated IP turnover and PGF2α release. Thus the induction of ovulation and the subsequent luteal phase in post-partum ewes is against a back ground of high oxytocin receptor expression and enhanced PGF2α release which in some ewes may contribute to abnormal luteal function. Journal of Endocrinology (1993) 136, 17–25

Expression of the IGF-II/mannose-6-phosphate receptor mRNA and protein in the developing rat

Development ◽  
1990 ◽  
Vol 109 (1) ◽  
pp. 67-73 ◽  
Author(s):  
P.V. Senior ◽  
S. Byrne ◽  
W.J. Brammar ◽  
F. Beck
Keyword(s):  
Skeletal Muscle ◽  
In Situ Hybridization ◽  
Receptor Expression ◽  
Receptor Mrna ◽  
Mrna Induction ◽  
Factor Ii ◽  
Mannose 6 Phosphate ◽  
Developing Rat ◽  
Lysosomal Transport

The insulin-like growth factor II (IGF-II) receptor is identical to the mannose-6-phosphate receptor (M-6-P), but its role as a somatomedin transducer is uncertain. IGF-II/M-6-P receptor expression was studied by in situ hybridization (ISH) in the developing rat. Expression occurs in extra-embryonic membranes at the time of IGF-II mRNA induction and later at paracrine/autocrine sites of IGF-II action (skeletal muscle and perichondrium) in the embryo. Highest levels of receptor mRNA occur in heart and major vessels. Postnatally transcription is strongly down-regulated. This suggests a role for the IGF-II/M-6-P receptor in IGF-II action or turnover during development distinct from its role in lysosomal transport.

Expression and clinical significance of Ki-67, oestrogen and progesterone receptors in acoustic neuroma

2007 ◽  
Vol 122 (2) ◽  
pp. 125-127 ◽  
Author(s):  
S Cafer ◽  
I Bayramoglu ◽  
N Uzum ◽  
M Yilmaz ◽  
L Memis ◽  
...  
Keyword(s):  
Progesterone Receptor ◽  
Acoustic Neuroma ◽  
Oestrogen Receptor ◽  
Hormone Receptors ◽  
Tumour Size ◽  
Progesterone Receptors ◽  
Inhibitory Effect ◽  
Receptor Expression ◽  
Ki 67 ◽  
Tissue Samples

AbstractObjective:The objective was to assess the presence of Ki-67, and oestrogen and progesterone hormone receptors as well as their clinical correlates in acoustic neuroma.Methods:Medical records of 59 patients who were operated on for acoustic neuroma between 1995 and 2003 were evaluated retrospectively. Formaldehyde-fixed paraffin-embedded archival acoustic neuroma specimens of the patients were used for immunohistochemical assessments of oestrogen and progesterone hormone receptors, and Ki-67 proliferative marker.Results:Tumour sizes were small (<19 mm), medium (20–39 mm) and large (>40 mm) in 21, 35 and 3 patients, respectively. On immunohistochemistry, all samples were (+) for progesterone receptor and (–) for oestrogen receptor staining. Ki-67 staining was encountered in 34 of 59 (57.6 per cent) patients, and Ki-67 values ranged from 0 per cent to 10.9 per cent (mean 1.36 per cent). There was no correlation between Ki-67, gender, tumour size and symptoms of the patients (p > 0.05).Conclusion:Oestrogen is not an important hormone in acoustic neuroma due to the absence of oestrogen receptor expression in the tissue samples. Since the progesterone receptor is expressed in all acoustic neuroma samples, further studies are necessary to find out about the inhibitory effect of antiprogesterone treatment on acoustic neuroma growth, which may be important particularly in elderly people or high-risk patients. Although Ki-67 is expressed in the majority of acoustic neuromas, it is not an important marker in clinical practice due to a lack of any correlation with the clinical parameters.

scholarly journals The clinical significance of oestrogen receptor expression in breast ductal carcinoma in situ

2020 ◽  
Vol 123 (10) ◽  
pp. 1513-1520 ◽  
Author(s):  
Islam M. Miligy ◽  
Michael S. Toss ◽  
Sho Shiino ◽  
Georgette Oni ◽  
Binafsha M. Syed ◽  
...  
Keyword(s):  
Clinical Significance ◽  
Ductal Carcinoma In Situ ◽  
Carcinoma In Situ ◽  
Oestrogen Receptor ◽  
Ductal Carcinoma ◽  
Receptor Expression

Preweaning Paternal Deprivation Impacts Parental Responses to Pups and Alters the Serum Oxytocin and Corticosterone Levels and Oxytocin Receptor, Vasopressin 1A Receptor, Oestrogen Receptor, Dopamine Type I Receptor, Dopamine Type II Receptor Levels in Relevant Brain Regions in Adult Mandarin Voles

2019 ◽  
Vol 110 (3-4) ◽  
pp. 292-306 ◽  
Author(s):  
Wei Yuan ◽  
Laifu Li ◽  
Wenjuan Hou ◽  
Zhixiong He ◽  
Limin Wang ◽  
...  
Keyword(s):  
Oestrogen Receptor ◽  
Maternal Separation ◽  
Brain Regions ◽  
Oxytocin Receptor ◽  
Type I ◽  
Type Ii ◽  
Receptor Mrna ◽  
Males And Females ◽  
Vasopressin 1A Receptor ◽  
Parental Responses

Although maternal separation and neonatal paternal deprivation (PD) have been found to exert a profound and persistent effects on the physiological and behavioural development of offspring, whether preweaning PD (PPD; from PND 10 to 21) affects maternal and parental responses to pups and the underlying neuroendocrine mechanism are under-investigated. Using monogamous mandarin voles (Microtus mandarinus), the present study found that PPD increased the latency to approach a pup-containing ball, decreased the total durations of sniffing and contacting a pup-containing ball and walking and increased the total duration of inactivity in both sexes. Moreover, PPD decreased serum oxytocin levels and increased corticosterone levels, but only in females. Furthermore, in both males and females, PPD decreased the expression of oxytocin receptor mRNA and protein in the medial preoptic area (MPOA), nucleus accumbens (NAcc) and medial prefrontal cortex (mPFC), but increased it in the medial amygdala (MeA) and decreased the expression of oestrogen receptor mRNA and protein in the MPOA. PPD increased the expression of dopamine type I receptor in the NAcc, but decreased it in the mPFC. PPD decreased dopamine type II receptor (D2R) in the NAcc both in males and females, but increased D2R in the mPFC in females and decreased D2R protein expression in males. Moreover, PPD decreased vasopressin 1A receptor (V1AR) in the MPOA, MeA and mPFC, but only in males. Our results suggest that the reduction of parental responses to pups induced by PPD may be associated with the sex-specific alteration of several neuroendocrine parameters in relevant brain regions.

Expression of GH receptor mRNA in regenerating skeletal muscle of normal and hypophysectomized rats. An in situ hybridization study

1991 ◽  
Vol 125 (6) ◽  
pp. 595-602 ◽  
Author(s):  
Eva Jennische ◽  
Göran L. Andersson
Keyword(s):  
Skeletal Muscle ◽  
Growth Hormone ◽  
In Situ Hybridization ◽  
Growth Hormone Receptor ◽  
Receptor Expression ◽  
Muscle Fibres ◽  
Receptor Mrna ◽  
Gh Receptor ◽  
Hypophysectomized Rats

Abstract. Expression of growth hormone receptor mRNA was investigated by in situ hybridization in skeletal muscle from normal and hypophysectomized rats during the first seven days of regeneration after ischemic injury. A digoxigenin-labelled RNA probe directed against the extracellular part of the rat GH receptor was used. In both normal and hypophysectomized rats distinct expression of GH receptor mRNA could be demonstrated in the regenerating muscle cells at the myoblast/myotube stage. The GH receptor expression appeared to decline with increasing maturation of the regenerated muscle fibres. In hypophysectomized rats, the regeneration process and the expression of GH receptor mRNA was delayed compared with that in normal animals. It is concluded that growth hormone may affect also the early phase of muscle regeneration in normal animals. To what extent lack of growth hormone contributes to the delayed regeneration observed in the hypophysectomized rats remains to be elucidated.

Distribution of cells containing progesterone receptor mRNA in the female rat di- and telencephalon: an in situ hybridization study

1992 ◽  
Vol 14 (3) ◽  
pp. 239-249 ◽  
Author(s):  
Kazuki Hagihara ◽  
Shuji Hirata ◽  
Takaaki Osada ◽  
Mitsuo Hirai ◽  
Junzo Kato
Keyword(s):  
In Situ Hybridization ◽  
Progesterone Receptor ◽  
Female Rat ◽  
Hybridization Study ◽  
Receptor Mrna

scholarly journals Lobular Carcinoma in Situ During Preoperative Biopsy and the Rate of Upgrade

2021 ◽  
Author(s):  
Jeea Lee ◽  
Ga Yoon Ku ◽  
Haemin Lee ◽  
Hyung Seok Park ◽  
Ja Seung Ku ◽  
...  
Keyword(s):  
Progesterone Receptor ◽  
Carcinoma In Situ ◽  
Invasive Carcinoma ◽  
Lobular Carcinoma ◽  
Receptor Expression ◽  
Progesterone Receptor Expression ◽  
Lobular Carcinoma In Situ ◽  
Final Pathology ◽  
Preoperative Biopsy

Abstract Background: There is a potential risk that lobular carcinoma in situ (LCIS) on preoperative biopsy might be diagnosed as ductal carcinoma in situ (DCIS) or invasive carcinoma in the final pathology. This study aimed to evaluate the rate of upgrade of LCIS on preoperative biopsy to DCIS or invasive carcinoma.Methods: Data of 55 patients with LCIS on preoperative biopsy were analyzed. All patients underwent surgery between 1991 and 2016 at Severance Hospital in Seoul, Korea. We analyzed the rate of upgrade of preoperative LCIS to DCIS or invasive cancer in the final pathology. The clinicopathologic features related to the upgrade were evaluated.Results: The rate of upgrade of LCIS to DCIS or invasive carcinoma was 16.4% (9/55). In multivariate analysis, microcalcification and progesterone receptor expression were significantly associated with the upgrade of LCIS (P = 0.023 and 0.044, respectively).Conclusions: The current study showed a relatively high rate of upgrade of LCIS on preoperative biopsy to DCIS or invasive cancer. The presence of microcalcification and progesterone receptor expression may be potential predictors of upgradation of LCIS on preoperative biopsy. Surgical excision of the LCIS during preoperative biopsy could be a management option to identify the concealed malignancy.

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