scholarly journals The Role of PEG Conformation in Mixed Layers: From Protein Corona Substrate to Steric Stabilization Avoiding Protein Adsorption.

2015 ◽  
Author(s):  
Víctor F. Puntes ◽  
Joan Comenge
Keyword(s):  
Physicochemical Properties ◽  
Protein Adsorption ◽  
Steric Hindrance ◽  
Protein Corona ◽  
Electrostatic Repulsion ◽  
Stabilization Mechanism ◽  
Biological Fate ◽  
Mixed Layers ◽  
Mixture Of Ligands

<p>Although nanoparticles have been traditionally modified with a single ligand layer, mixture of ligands might help to combine different functionalities and to further engineer the NP surface. A detailed study of the competition between an alkanethiol (11-mercaptoundecanoic acid) and SH-PEG for the surface of AuNPs and the resultant behaviors of this model nanoconjugate is presented here. As a result, the physicochemical properties of these conjugates can be progressively tuned by controlling the composition and especially the conformation of the mixed monolayer. This has implications in the physiological stability. The controlled changes on the SH-PEG conformation rather than its concentration induces a change in the stabilization mechanism from electrostatic repulsion to steric hindrance, which changes the biological fate of NPs. Importantly, the adsorption of proteins on the conjugates can be tailored by tuning the composition and conformation of the mixed layer.</p>

Recent advances in the analysis of nanoparticle-protein coronas

Nanomedicine ◽  
2020 ◽  
Vol 15 (10) ◽  
pp. 1037-1061
Author(s):  
Muhammad Ovais ◽  
Susheel Kumar Nethi ◽  
Saleem Ullah ◽  
Irshad Ahmad ◽  
Sudip Mukherjee ◽  
...  
Keyword(s):  
Protein Corona ◽  
Recent Decade ◽  
The Body ◽  
Pharmacokinetics And Pharmacodynamics ◽  
Physiological Factors ◽  
Characterization Methods ◽  
Biological Fate ◽  
Circulation Distribution ◽  
The Impact

In spite of radical advances in nanobiotechnology, the clinical translation of nanoparticle (NP)-based agents is still a major challenge due to various physiological factors that influence their interactions with biological systems. Recent decade witnessed meticulous investigation on protein corona (PC) that is the first surrounds NPs once administered into the body. Formation of PC around NP surface exhibits resilient effects on their circulation, distribution, therapeutic activity, toxicity and other factors. Although enormous literature is available on the role of PC in altering pharmacokinetics and pharmacodynamics of NPs, understanding on its analytical characterization methods still remains shallow. Therefore, the current review summarizes the impact of PC on biological fate of NPs and stressing on analytical methods employed for studying the NP-PC.

Toxicity of engineered nanomaterials with different physicochemical properties and the role of protein corona on cellular uptake and intrinsic ROS production

Toxicology ◽  
2020 ◽  
Vol 442 ◽  
pp. 152545
Author(s):  
Alejandro Déciga-Alcaraz ◽  
Estefany I. Medina-Reyes ◽  
Norma L. Delgado-Buenrostro ◽  
Carolina Rodríguez-Ibarra ◽  
Adriana Ganem-Rondero ◽  
...  
Keyword(s):  
Physicochemical Properties ◽  
Cellular Uptake ◽  
Protein Corona ◽  
Engineered Nanomaterials ◽  
Ros Production

The role of morphology, shell composition and protein corona formation in Au/Fe3O4 composite nanoparticle mediated macrophage responses

2021 ◽  
Author(s):  
Lihua He ◽  
Kang Ma ◽  
Xiaonan Liu ◽  
Huixia Li ◽  
Lei Zhang ◽  
...  
Keyword(s):  
Immune System ◽  
Physicochemical Properties ◽  
Biomedical Applications ◽  
Protein Corona ◽  
System P ◽  
Corona Formation ◽  
Composite Nanoparticle ◽  
Shell Composition

The great interest in using nanoparticles (NPs) for biomedical applications is transversal to various materials despite the poorly understood correlation between their physicochemical properties and effects on the immune system....

Characteristics Of The Structure Formation Of Gypsum Binder Modified With Zinc Hydrosilicates

2020 ◽  
pp. 40-46
Keyword(s):  
Synergistic Effect ◽  
Physicochemical Properties ◽  
Structure Formation ◽  
Silicic Acid ◽  
Chemical Properties ◽  
Crystal Formation ◽  
Ability To Act ◽  
Chemical Factors ◽  
Sorption Characteristics

The authors' methodic for assessing the role of chemical and physic-chemical factors during the structure formation of gypsum stone is presented in the article. The methodic is also makes it possible to reveal the synergistic effect and to determine the ranges of variation of controls factors that ensure maximum values of such effect. The effect of a micro-sized modifier based on zinc hydro-silicates on the structure formation of building gypsum is analyzed and corresponding dependencies are found. It is shown that effects of influence of modifier on the properties of gypsum compositions are determined by chemical properties of modifier. Among the mentioned properties are sorption characteristics (which depend on the amount of silicic acid and its state) and physicochemical properties - the ability to act as a substrate during crystal formation. The proposed method can also be extended to other binding substances and materials. This article contributes to the understanding of the processes that occur during the structure formation of composites, which will make it possible to control the structure formation in the future, obtaining materials with a given set of properties.

scholarly journals A Proteomic Study on the Personalized Protein Corona of Liposomes. Relevance for Early Diagnosis of Pancreatic DUCTAL Adenocarcinoma and Biomarker Detection

2021 ◽  
Vol 2 (2) ◽  
pp. 82-93
Author(s):  
Luca Digiacomo ◽  
Francesca Giulimondi ◽  
Daniela Pozzi ◽  
Alessandro Coppola ◽  
Vincenzo La Vaccara ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Early Diagnosis ◽  
Pancreatic Ductal Adenocarcinoma ◽  
Protein Corona ◽  
Detection Sensitivity ◽  
Ductal Adenocarcinoma ◽  
Protein Biomarkers ◽  
Ca 19.9 ◽  
Proteomic Study

Due to late diagnosis, high incidence of metastasis, and poor survival rate, pancreatic cancer is one of the most leading cause of cancer-related death. Although manifold recent efforts have been done to achieve an early diagnosis of pancreatic cancer, CA-19.9 is currently the unique biomarker that is adopted for the detection, despite its limits in terms of sensitivity and specificity. To identify potential protein biomarkers for pancreatic ductal adenocarcinoma (PDAC), we used three model liposomes as nanoplatforms that accumulate proteins from human plasma and studied the composition of this biomolecular layer, which is known as protein corona. Indeed, plasma proteins adsorb on nanoparticle surface according to their abundance and affinity to the employed nanomaterial, thus even small differences between healthy and PDAC protein expression levels can be, in principle, detected. By mass spectrometry experiments, we quantified such differences and identified possible biomarkers for PDAC. Some of them are already known to exhibit different expressions in PDAC proteomes, whereas the role of other relevant proteins is still not clear. Therefore, we predict that the employment of nanomaterials and their protein corona may represent a useful tool to amplify the detection sensitivity of cancer biomarkers, which may be used for the early diagnosis of PDAC, with clinical implication for the subsequent therapy in the context of personalized medicine.

Role of association on protein adsorption isotherms

1989 ◽  
Vol 482 (1) ◽  
pp. 1-12 ◽  
Author(s):  
R. Blanco ◽  
A. Arai ◽  
N. Grinberg ◽  
M. Yarmush ◽  
B.L. Karger
Keyword(s):  
Protein Adsorption ◽  
Adsorption Isotherms

scholarly journals The Role of H2C2O4 and Na2CO3 as Precipitating Agents on The Physichochemical Properties and Photocatalytic Activity of Bismuth Oxide

2020 ◽  
Vol 18 (1) ◽  
pp. 129-137
Author(s):  
Yayuk Astuti ◽  
Rizka Andianingrum ◽  
Abdul Haris ◽  
Adi Darmawan ◽  
Keyword(s):  
Photocatalytic Activity ◽  
Methyl Orange ◽  
Physicochemical Properties ◽  
Bismuth Oxide ◽  
Rate Constants ◽  
Degradation Rate ◽  
Precipitation Method ◽  
Methyl Orange Degradation ◽  
Methyl Orange Dye

AbstractSynthesis of bismuth oxide synthesis through the precipitation method using H2C2O4 and Na2CO3 precipitating agents, identification of physicochemical properties and its photocatalysis activity for methyl orange degradation were conducted. The bismuth oxide synthesis was undertaken by dissolving Bi(NO3)3.5H2O in HNO3, then added precipitating agents to form precipitate. The results showed that bismuth oxide produced by H2C2O4 precipitating agent was a yellow powder containing a mixture of α-Bi2O3 (monoclinic) and β-Bi2O3 (tetragonal), porous with size of 28-85 μm. Meanwhile, the use of Na2CO3 as precipitating agent resulted in bismuth oxide consisting of α-Bi2O3 and β-Bi2O3 and Bi2O4, irregular shape without pore being 40-115 μm in size. Bismuth oxide synthesized with H2C2O4 precipitating agent showed higher photocatalytic activity compared to bismuth oxide synthesized using Na2CO3 on degrading methyl orange dye with degradation rate constants of 2.35x10-5 s-1 for H2C2O4 and 1.81x10-5 s-1 for Na2CO3.

Cell adhesion on chiral surface: The role of protein adsorption

2012 ◽  
Vol 90 ◽  
pp. 97-101 ◽  
Author(s):  
Feng Zhou ◽  
Lin Yuan ◽  
Dan Li ◽  
He Huang ◽  
Taolei Sun ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Protein Adsorption ◽  
Chiral Surface
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