scholarly journals IN VITRO ANTIMICROBIAL ACTIVITY OF COCONUT SHELL LIQUID SMOKE AGAINST Candida albicans AND Lactobacillus acidophilus ATTRIBUTING TO ENDODONTIC TREATMENT FAILURE

2021 ◽  
Vol 10 (1) ◽  
pp. 35-40
Author(s):  
Isniya Nosartika ◽  
Nadia Hardini ◽  
Tyas Prihatiningsih
Keyword(s):  
Antimicrobial Activity ◽  
Treatment Failure ◽  
Root Canal ◽  
Endodontic Treatment ◽  
Coconut Shell ◽  
Dilution Method ◽  
Liquid Smoke ◽  
Significant Difference ◽  
Post Hoc

L. acidophilus bacteria and C. albicans fungi are the most common microorganisms isolated from the oral cavity of patients suffering from post-treatment of root canal endodontic infections. Chlorophenol Camphor menthol (ChM) is one of the antimicrobials used for root canal sterilizer but it still has many flaws. Coconut shell liquid smoke (CS-LS) is a natural product that has an antimicrobial activity and non-toxic chemical compounds for humans. This study aims at determining the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of L. acidophilus bacteria and C. Albicans fungi and comparing the antimicrobial activity between CS-LC with commercial root canal sterilization. The MIC and MBC of CS-LS were measured using 10 different concentrations ranging from 10-100%. MIC was determined by the broth dilution method. All concentration was cultured on each agar media to determine its MBC. The data were analyzed using the comparative test of Kruskall-Wallis with post-hoc Mann-Whitney. MIC and MBC of C. albicans were 10% of CSLS. Meanwhile, L. acidophilus has the MIC with a 50% concentration of CSLS and MBC with a 60% concentration of CS-LS. No significant difference in CS-LS and ChKM was found. Thus, CS-LS has a potential for endodontic treatment failure. Further research should be conducted to know other potential uses of CS-LS in dentistry.

scholarly journals Evaluation of Cyclic Fatigue of Hyflex EDM, Twisted Files, and ProTaper Gold Manufactured with Different Processes: An In Vitro Study

2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
Pooja D. Khandagale ◽  
Prashant P. Shetty ◽  
Saleem D. Makandar ◽  
Pradeep A. Bapna ◽  
Mohmed Isaqali Karobari ◽  
...  
Keyword(s):  
Fatigue Resistance ◽  
Root Canal ◽  
File Systems ◽  
In Vitro Study ◽  
Cyclic Fatigue ◽  
Segment Length ◽  
Significant Difference ◽  
Number Of Cycles ◽  
Post Hoc

Introduction. The main aims of root canal instrumentation are to provide an environment that will lead to healing and to provide a root canal shape that is comfortable to clean and seal. When working with rotary endodontic instruments, the most significant concerns are that the instrument might fracture in the root canal, thus affecting the treatment outcome. Hence, it is of immense importance to know which file systems have more cyclic fatigue resistance. Methodology. This study evaluated the effect of the curved segment length of the artificial canal (the arch), and the number of cycles necessary in fracture of Hyflex EDM, Twisted files, and ProTaper Gold were recorded. Sixty NiTi rotary instruments of 25 mm length (Hyflex EDM (20), Twisted files (20), and ProTaper Gold (20)) were tested in a metal block with simulated canal having 90° angle of curvature. The study was performed with a specific radius and degree of curvature, i.e., 8 mm radius and 90⁰ angle of curvature, and data obtained were subsequently subjected to statistical evaluation using one-way analysis of variance and Tukey’s post hoc test. Result. The Hyflex EDM (774.29) exhibited the maximum cyclic fatigue resistance compared to Twisted files (654.875) and ProTaper Gold (375.575). A statistically significant difference was observed between the tested groups. Conclusion. The Hyflex EDM files showed the highest cyclic fatigue resistance, followed by Twisted files and ProTaper Gold files.

scholarly journals Impacts of contracted endodontic cavities compared to traditional endodontic cavities in premolars

2020 ◽  
Author(s):  
Xia Juan ◽  
Wang weidong ◽  
Li Zhengmao ◽  
Lin Bingpeng ◽  
Zhang Qian ◽  
...  
Keyword(s):  
Root Canal ◽  
Fracture Resistance ◽  
Testing Machine ◽  
Filling Material ◽  
Root Canal Filling ◽  
Surface Areas ◽  
Significant Difference ◽  
3D Printed ◽  
Post Hoc

Abstract Background: This study aims to compare the percentage of dentin removed, instrumentation efficacy, root canal filling and load at fracture between contracted endodontic cavities, and traditional endodontic cavities on root canal therapy in premolars. Methods: Forty extracted intact human first premolars were imaged with micro-CT and randomly assigned to the contracted endodontic cavity (CEC) or traditional endodontic cavity (TEC) groups. CEC was prepared with the aid of a 3D-printed template, canals were prepared with a 0.04 taper M-Two rotary instrument, and cavities were restored with resin. Specimens were loaded to fracture in an Instron Universal Testing Machine after a fatigue phase. The data were analyzed by the independent samples T test and Mann-Whitney U test, appropriate post hoc tests. Results: In the premolars tested in vitro, the percentage of dentin removed in the premolars with two dental roots in the CEC group (3.85% ± 0.42%) was significantly smaller (P < 0.05) than in the TEC group (4.94% ± 0.5%). The untouched canal wall (UCW) after instrumentation for TECs (16.43% ± 6.56%) was significantly lower (P< .05) than the UCW (24.42% ± 9.19%) for CECs in single-rooted premolars. No significant differences were observed in the increased canal volume and surface areas in premolars between the TEC and CEC groups (P > 0.05). CECs conserved coronal dentin in premolars with two dental roots but no impact on the instrument efficacy. There were no differences between the CEC groups and the TEC groups in the percentage of filling material and voids (P > 0.05). In addition, the mean load at failure of premolars did not significantly differ between the CEC and TEC groups and there was no significant difference in the type of fracture (P > 0.05). Conclusion: The results of this study suggest that CEC could not improve the fracture resistance of the endodontically treated premolars. The instrumentation efficacy and the percentage of filling material did not significantly differ between CECs and TECs in premolars. Keywords: 3D-printed template, contracted endodontic cavities, instrumentation efficacy, root canal filling, fracture resistance

scholarly journals Impacts of contracted endodontic cavities compared to traditional endodontic cavities in premolars

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Juan Xia ◽  
Weidong Wang ◽  
Zhengmao Li ◽  
Bingpeng Lin ◽  
Qian Zhang ◽  
...  
Keyword(s):  
Root Canal ◽  
Testing Machine ◽  
Filling Material ◽  
Root Canal Filling ◽  
Surface Areas ◽  
Significant Difference ◽  
3D Printed ◽  
Post Hoc ◽  
Rotary Instrument

Abstract Background This study aims to compare the percentage of dentin removed, instrumentation efficacy, root canal filling and load at fracture between contracted endodontic cavities, and traditional endodontic cavities on root canal therapy in premolars. Methods Forty extracted intact human first premolars were imaged with micro-CT and randomly assigned to the contracted endodontic cavity (CEC) or traditional endodontic cavity (TEC) groups. CEC was prepared with the aid of a 3D-printed template, canals were prepared with a 0.04 taper M-Two rotary instrument, and cavities were restored with resin. Specimens were loaded to fracture in an Instron Universal Testing Machine after a fatigue phase. The data were analyzed by the independent samples T test and Mann-Whitney U test, appropriate post hoc tests. Results In the premolars tested in vitro, the percentage of dentin removed in the premolars with two dental roots in the CEC group (3.85% ± 0.42%) was significantly smaller (P < 0.05) than in the TEC group (4.94% ± 0.5%). The untouched canal wall (UCW) after instrumentation for TECs (16.43% ± 6.56%) was significantly lower (P < .05) than the UCW (24.42% ± 9.19%) for CECs in single-rooted premolars. No significant differences were observed in the increased canal volume and surface areas in premolars between the TEC and CEC groups (P > 0.05). CECs conserved coronal dentin in premolars with two dental roots but no impact on the instrument efficacy. There were no differences between the CEC groups and the TEC groups in the percentage of filling material and voids (P > 0.05). In addition, the mean load at failure of premolars did not significantly differ between the CEC and TEC groups and there was no significant difference in the type of fracture (P > 0.05). Conclusion The results of this study suggest that CEC could not improve the fracture resistance of the endodontically treated premolars. The instrumentation efficacy and the percentage of filling material did not significantly differ between CECs and TECs in premolars.

scholarly journals Prevalence of Three-Rooted Mandibular First Molars among Indians Using SCT

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Amit Kumar Garg ◽  
Rajendra Kumar Tewari ◽  
Neha Agrawal
Keyword(s):  
Treatment Failure ◽  
Root Canal ◽  
Dental Treatment ◽  
Endodontic Treatment ◽  
Root Canals ◽  
Significant Difference ◽  
High Prevalence ◽  
Root Canal System ◽  
The Right ◽  
Mandibular First Molar

Undetected extra roots or root canals are a major reason for failure of endodontic treatment. Failure to recognize an extra distolingual (DL) root in mandibular first molar may lead to incomplete debridement of the root canal system and eventually treatment failure. Therefore, it is crucial that atypical anatomy is identified before and during dental treatment. Spiral computed tomography (SCT) images can show 3D images, and therefore much detail can be used when traditional methods prevent adequate endodontic treatment. The overall incidence of DL roots on the mandibular first molars was 6.40% for all patients and 5.00% for all teeth, respectively. The occurrence of DL roots on the right side and on the left side showed a statistically significant difference. The bilateral incidence of symmetrical distribution of DL roots was 56.25%. The DL root canal orifice was separated from DB canal orifice by2.79±0.34 mm, from the MB canal orifice by4.23±0.81 mm, and from the ML canal orifice by3.29±0.52 mm. The high prevalence of the DL root in permanent mandibular first molars among the Indian population by using SCT and estimations of the interorifice distance of such teeth might be useful for successful endodontic treatments.

An Analysis of the Antimicrobial Activity of Ten Root Canal Sealers - A Duration Based in vitro Evaluation

2008 ◽  
Vol 33 (2) ◽  
pp. 117-122 ◽  
Author(s):  
Prasanna Neelakantan ◽  
Subbarao CV
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Enterococcus Faecalis ◽  
Root Canal ◽  
Antimicrobial Action ◽  
Agar Diffusion Test ◽  
Significant Difference ◽  
Ah Plus ◽  
Root Canal Sealers

Aim and Design: Sealers with prolonged antimicrobial activity are needed by the fact that microorganisms are commonly recovered from teeth with failed endodontic therapy. The aim of this study was to evaluate the duration of antimicrobial activity of ten root canal sealers (Apexit plus, Tubli Seal Xpress, Endoflas FS,Endomethasone, Endomethasone N, AH Plus, Epiphany, EndoRez, Ketac Endo, Roeko Seal) against Enterococcus faecalis and Candida albicans by the agar diffusion test. The zones of inhibition were examined immediately and after 24, 48, 72 hours, 5 and 7 days. Statistical analysis was done using Kruskal - Wallis test and Mann Whitney U test. Results and conclusions: Against Enterococcus faecalis, Endoflas FS showed the largest inhibitory zones immediately and 24 hours after manipulation, whereas, there was no significant difference between Endoflas FS and Endomethasone after 48 hours. Against Candida albicans, Endoflas FS performed better than the other sealers. All the sealers (except AH Plus, Epiphany and Roeko Seal) demonstrated higher antimicrobial action in the first 24 hours after manipulation The antimicrobial action of all the sealers (except AH Plus and Roeko Seal which showed no antimicrobial activity in any studied time and Epiphany which ceased to show any antimicrobial action after 24 hours) decreased significantly with time.

scholarly journals Impacts of contracted endodontic cavities compared to traditional endodontic cavities in premolars

2020 ◽  
Author(s):  
Xia Juan ◽  
Wang weidong ◽  
Li Zhengmao ◽  
Lin Bingpeng ◽  
Zhang Qian ◽  
...  
Keyword(s):  
Root Canal ◽  
Testing Machine ◽  
Filling Material ◽  
Root Canal Filling ◽  
Surface Areas ◽  
Significant Difference ◽  
3D Printed ◽  
Post Hoc ◽  
Rotary Instrument

Abstract Background: This study aims to compare the percentage of dentin removed, instrumentation efficacy, root canal filling and load at fracture between contracted endodontic cavities, and traditional endodontic cavities on root canal therapy in premolars. Methods: Forty extracted intact human first premolars were imaged with micro-CT and randomly assigned to the contracted endodontic cavity (CEC) or traditional endodontic cavity (TEC) groups. CEC was prepared with the aid of a 3D-printed template, canals were prepared with a 0.04 taper M-Two rotary instrument, and cavities were restored with resin. Specimens were loaded to fracture in an Instron Universal Testing Machine after a fatigue phase. The data were analyzed by the independent samples T test and Mann-Whitney U test, appropriate post hoc tests. Results: In the premolars tested in vitro, the percentage of dentin removed in the premolars with two dental roots in the CEC group (3.85% ± 0.42%) was significantly smaller (P < 0.05) than in the TEC group (4.94% ± 0.5%). The untouched canal wall (UCW) after instrumentation for TECs (16.43% ± 6.56%) was significantly lower (P< .05) than the UCW (24.42% ± 9.19%) for CECs in single-rooted premolars. No significant differences were observed in the increased canal volume and surface areas in premolars between the TEC and CEC groups (P > 0.05). CECs conserved coronal dentin in premolars with two dental roots but no impact on the instrument efficacy. There were no differences between the CEC groups and the TEC groups in the percentage of filling material and voids (P > 0.05). In addition, the mean load at failure of premolars did not significantly differ between the CEC and TEC groups and there was no significant difference in the type of fracture (P > 0.05). Conclusion: The results of this study suggest that CEC could not improve the fracture resistance of the endodontically treated premolars. The instrumentation efficacy and the percentage of filling material did not significantly differ between CECs and TECs in premolars.

ETHANOLIC EXTRACTS OF BHRINGRAJ, HARITAKI AND SHANKAPUSHPAM VS CHLORHEXIDINE GLUCONATE AGAINST S. MUTANS: AN IN VITRO ANALYSIS

2021 ◽  
pp. 12-14
Author(s):  
Hiranmayi Shivajirao Brid ◽  
Shivaprakash P. K. ◽  
Kishore G. Bhat
Keyword(s):  
Antimicrobial Activity ◽  
Ethanolic Extract ◽  
Extraction Process ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Ethanolic Extracts ◽  
Agar Dilution ◽  
Vitro Analysis ◽  
Post Hoc

Aim: To evaluate the antimicrobial efcacy of ethanolic extracts of Bhringraj, Haritaki and Shankapushpam against S. mutans and compare with commercially available 0.2% Chlorhexidene gluconate (CHX). Materials And Methods: Ethanolic extracts of Bhringraj, Haritaki and Shankapushpam were obtained by subjecting them to hot continuous extraction process and test extracts of 25%, 12.5%, 6.25% and 3.12% concentrations were prepared. Their antimicrobial efcacy was evaluated and compared with 0.2% CHX against S. mutans using the agar dilution method. The results were statistically analyzed using 1-way ANOVA and LSD Post Hoc tests. Results: Haritaki seemed to have superior and statistically signicant antimicrobial activity when compared to the test extracts at all the concentrations at which they were tested. It was followed by 0.2% CHX, which also showed considerable antimicrobial activity even at a low concentration. Conclusion: Ethanolic extract of Haritaki has better antimicrobial efcacy compared to 0.2% CHX at the tested concentrations and can be considered as a safe herbal alternative for it.

scholarly journals Impacts of contracted endodontic cavities compared to traditional endodontic cavities in premolars

2019 ◽  
Author(s):  
Xia Juan ◽  
Wang weidong ◽  
Li Zhengmao ◽  
Lin Bingpeng ◽  
Zhang Qian ◽  
...  
Keyword(s):  
Root Canal ◽  
Fracture Resistance ◽  
Testing Machine ◽  
Filling Material ◽  
Root Canal Filling ◽  
Surface Areas ◽  
Significant Difference ◽  
3D Printed ◽  
Post Hoc

Abstract Background: This study aims to compare the percentage of dentin removed, instrumentation efficacy, root canal filling and load at fracture between contracted endodontic cavities, and traditional endodontic cavities on root canal therapy in premolars.Methods: Forty extracted intact human first premolars were imaged with micro-CT and randomly assigned to the contracted endodontic cavity (CEC) or traditional endodontic cavity (TEC) groups. CEC was prepared with the aid of a 3D-printed guiding template, canals were prepared with a 0.04 taper M-Two rotary instrument, and cavities were restored with resin. The specimens were loaded to fracture in an Instron Universal Testing Machine after a fatigue phase. The data were analyzed by the independent samples T test and Mann-Whitney U test, appropriate post hoc tests.Results: In the premolars tested in vitro, the percentage of dentin removed in the premolars with two dental roots in the CEC group (3.85% ± 0.42%) was significantly smaller (P < 0.05) than in the TEC group (4.94% ± 0.5%). The untouched canal wall (UCW) after instrumentation for TECs (16.43% ± 6.56%) was significantly lower (P< .05) than the UCW (24.42% ± 9.19%) for CECs in single-rooted premolars. No significant differences were observed in the increased canal volume and surface areas in premolars between the TEC and CEC groups (P > 0.05). CECs conserved coronal dentin in premolars with two dental roots but no impact on the instrument efficacy, the root canal filling or the biomechanical responses was observed. There were no differences between the CEC groups and the TEC groups in the percentage of filling material and voids (P > 0.05). In addition, the mean load at failure of premolars did not significantly differ between the CEC and TEC groups and there was no significant difference in the type of fracture (P > 0.05).Conclusion: The results of this study suggest that CEC could not improve the fracture resistance of the endodontically treated premolars. The instrumentation efficacy and the percentage of filling material did not significantly differ between CECs and TECs in the premolars.Keywords: 3D-printed template, contracted endodontic cavities, instrumentation efficacy, root canal filling, fracture resistance

scholarly journals In vitro evaluation of the antimicrobial activity of five root canal sealers

2004 ◽  
Vol 15 (1) ◽  
pp. 30-35 ◽  
Author(s):  
Brenda Paula Figueiredo de Almeida Gomes ◽  
José Assis Pedroso ◽  
Rogério Castilho Jacinto ◽  
Morgana Eli Vianna ◽  
Caio Cezar Randi Ferraz ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Properties ◽  
Contact Method ◽  
Streptococcus Sanguis ◽  
Actinomyces Naeslundii ◽  
Agar Diffusion Test ◽  
Significant Difference ◽  
Ah Plus ◽  
Endodontic Sealers

The aim of the present study was to analyze the antimicrobial properties of five endodontic sealers: Endo Fill, Endomethasone, Endomethasone N, Sealer 26 and AH-Plus, against the following microorganisms: Candida albicans, Staphylococcus aureus, Enterococcus faecalis, Streptococcus sanguis and Actinomyces naeslundii. The sealers were tested immediately, 24 h, 48 h and 7 days after manipulation.The direct contact method through the observation of the microbial growth in liquid medium and the agar diffusion test were used to evaluate the antimicrobial properties of the sealers. The results, in both methodologies used, showed that immediately after manipulation, Endo-Fill and Endomethasone demonstrated the highest antimicrobial activity, with no statistically significant difference between them. Sealer 26 demonstrated the lowest antimicrobial activity. At all other times after manipulation, there were no statistically significant differences among all the sealers tested. In conclusion, none of the sealers totally inhibited the growth of the microorganisms. Furthermore, the antimicrobial activity of each sealer decreased with time and was dependent upon the microbial susceptibility to them.

scholarly journals The Effect of the Bioactive Glass and the Er:YAG Laser on the Remineralization of the Affected Dentin: A Comparative In Vitro Study

2020 ◽  
Vol 11 (2) ◽  
pp. 160-166
Author(s):  
Mohammad Javad Moghaddas ◽  
Horieh Moosavi ◽  
Sara Yaghoubirad ◽  
Nasim Chiniforush
Keyword(s):  
Bioactive Glass ◽  
In Vitro Study ◽  
Control Group ◽  
Dentinal Tubule ◽  
Glass Ionomer ◽  
Significant Difference ◽  
Post Hoc ◽  
Caries Lesions ◽  
Cavity Floor

Introduction: The purpose of this study was to compare the effect of the bioactive glass, the glass ionomer, and the Erbium YAG laser as liners on the remineralization of the affected dentin. Methods: The present study was conducted on 64 healthy extracted human molars divided into 4 groups, 1 control group and 3 experimental groups. After artificially inducing dentinal caries lesions, each of the experimental groups was applied to the cavity floor and then restored with a composite. The samples were stored after thermocycling in an incubator for two months. Finally, the hardness of the cavity floor was measured at 3 depths of 20, 50 and 100 μm by the Vickers microhardness tester. The dentin conditions underneath the liners were also evaluated with FESEM. Statistical analysis was performed by two-way ANOVA and the post-hoc Games-Howell test (P<0.05). Results: Among the groups, the lowest microhardness value was in the control group (P<0.05) except at a depth of 100 μm; therefore, there was no significant difference between the control group and the bioactive glass (P>0.05). The laser group had the highest microhardness value, which was significantly different from the control group (P<0.05). There was a significant difference between the laser and bioactive glass (P<0.05), except at a depth of 20 μm. The laser and glass ionomer had only a significant difference at a depth of 100 μm (P<0.05). The microhardness value induced by glass ionomer was higher than bioactive glass, which in no depth was significant (P>0.05). Partial dentinal tubule occlusion was observed with FESEM in each of the experimental groups as compared to the control group. Conclusion: The microhardness values were higher in all groups than in the control group. The laser might be more successful in remineralization than the other ones.

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