scholarly journals Jenis Pelarut Metanol Dan N-Heksana Terhadap Aktivitas Antioksidan Ekstrak Rumput Laut Gelidium sp. Dari Pantai Drini Gunungkidul – Yogyakarta

2018 ◽  
Vol 21 (1) ◽  
pp. 9
Author(s):  
Wahyu Bagio Leksono ◽  
Rini Pramesti ◽  
Gunawan Widi Santosa ◽  
Wilis Ari Setyati
Keyword(s):  
Antioxidant Activity ◽  
Chlorophyll A ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Red Seaweed ◽  
Rotary Evaporator ◽  
Separatory Funnel ◽  
Ic50 Value ◽  
The One

AbstractType of Solvent Methanol And N-Hexane Against Antioxidant Activity Seaweed Extract Gelidium sp. From Drini Beach Gunungkidul – Yogyakarta             Gelidium sp. the one of red seaweed which has potential as naturalantioxidant. The researh was to know the activity of methanol extract, n-hexane Gelidium sp., and determined totalphenolic compound of pigments (chlorophyll a and carotenoid). Decriptiveexplorative method was used in this research and it was taken from Drini Beach, Gunungkidul, Yogyakarta. Maseration to this sample was done by methanol as solvent, evaporated by rotary evaporator and partitied by n-hexane solvent with separatoryfunnel. Antioxidant activity was determined by transfer electron method using DPPH (1,1-diphenyl-2-picrylhidrazyl) as free radicals. Totalphenolic compound being tested using Folin-Ciocalteu solution with gallic acid as standard and measured at a wavelength of 725 nm, while the chlorophylls were spectrophotometry method and measured at a wavelength of 663 nm and 646 nm as well as carotenoidswere measured at a wavelength of 470 nm. The results showed that IC50 value of methanol extract was 340,10 ppm and n-hexane was 66,25 ppm. IC50 value of methanol extract was categorized as very weak in antioxidant activity, while n-heksan extract was categorized as strong. Total phenolic content in each extract were 46,55 and 135,62 (mg GAE/g extract), chlorophyll a 8,47 and 10,88 (mg/g extract sample) and carotenoids 50,38 and 84,27 (μmol/g extract sample). Keywords: Gelidium sp., Antioxidant, DPPH Abstrak  Gelidium sp. merupakan salah satu rumput laut merah yang berpotensi sebagai sumber antioksidan alami. Penelitian ini bertujuan untuk mengetahui aktivitas antioksidan ekstrak metanol dan n-heksan Gelidium sp. (segar), menentukan kadar total fenolat dan pigmen (klorofil a dan karotenoid). Metode deskriptif eksploratif digunakan dalam penelitian ini dan diperoleh diambil dari Pantai Drini, Gunungkidul, Yogyakarta dibersihkan dengan air tawar, dimaserasi dengan pelarut metanol, diuapkan dengan rotary evaporator dan dipartisi dengan pelarut n-heksan menggunakan corong pemisah (separatory funnel). Aktivitas antioksidan ditentukan dengan metode transfer elektron menggunakan DPPH (1,1-diphenyl-2-picrylhidrazyl) sebagai radikal bebas. Kadar total fenolat diuji dengan metode Folin-Ciocalteu dengan asam galat sebagai larutan standar dan diukur pada panjang gelombang 725 nm. Kadar klorofil a diukur dengan metode spektrofotometri pada panjang gelombang 663 nm dan 646 nm sedangkan kadar karotenoid diukur pada panjang gelombang 470 nm. Hasil penelitian menunjukkan nilai IC50 ekstrak metanol sebesar 340,10 ppm dan ekstrak n-heksan 66,25 ppm. Nilai IC50ekstrak metanol termasuk kategori aktivitas antioksidan sangat lemah sedangkan n-heksana termasuk kategori kuat. Kadar total fenolat pada masing-masing ekstrak 46,55 dan 135,62 (mg GAE/g ekstrak), kadar klorofil a sebesar 8,47 dan 10,88 (mg/g sampel) dan kadar karotenoid sebesar 50,38 dan 84,27 (µ mol/g sampel).Kata kunci: Gelidium sp., Antioksidan, DPPH

scholarly journals Kandungan Pigmen, Total Fenolik Dan Aktivitas Antioksidan Sargassum sp.

2018 ◽  
Vol 21 (2) ◽  
pp. 137
Author(s):  
Sri Sedjati ◽  
Endang Supriyantini ◽  
Ali Ridlo ◽  
Nirwani Soenardjo ◽  
Victorina Yulina Santi
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Chlorophyll A ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Total Phenolic ◽  
Chlorophyll B ◽  
Total Phenolic Compounds ◽  
Total Phenolic Compound ◽  
Ic50 Value

Pigmen content, total phenolic compound and antioxidant activity Sargassum sp.   Sargassum sp. contains secondary metabolites which potentially act as natural source of antioxidant. The purpose of this study was to determine bioactive contents (pigments, total phenolic compounds), and antioxidant activities of Sargassum sp. The method of this research is descriptive-explorative.  Sample was extracted with methanol, while pigments extraction with aseton 80%.  Chlorophyll a, b and carotenoids were carried out with spectrophotometer method. The total phenolic compounds were analyzed by spectrophotometer method using Follin-Ciocalteu reagent, and antioxidant activities were measured using DPPH (1,1-diphenyl-2- picrylhidrazyl) method. The result of this research showed that methanol extract of Sargassum sp. had the presence of chlorophyll a 2.84 mg/g, chlorophyll b 1.15 mg/g, and carotenoids 2.63 μmol/g sample. The total phenolic compounds of methanol extract were 57.97 mg GAE/g sample. Methanol extract of Sargassum sp. was showing a strong antioxidant activity that could be seen in IC50 value (69,27 ppm) in less than 100 ppm concentration. Sargassum sp. mengandung beberapa metabolit sekunder yang berpotensi sebagai sumber antioksidan alami. Tujuan dari penelitian ini adalah mengkaji komponen bioaktif (pigmen, total fenolik) dan aktivitas antioksidan dari rumput laut Sargassum sp. Metoda penelitian ini adalah diskriptif eksploratif.  Sampel Sargassum sp. basah diekstraksi dengan cara maserasi dengan pelarut  metanol, sedangkan ekstraksi pigmen menggunakan aseton 80%. Penentuan kadar pigmen (klorofil a, b, karotenoid ), total fenolik dan aktivitas antioksidan berdasarkan metode spektrofotometri. Total fenolik diukur berdasarkan uji Follin-Ciocalteu, dan   aktivitas antioksidan  berdasarkan uji DPPH (1,1-diphenyl-2- picrylhidrazyl). Hasil penelitian menunjukan bahwa ekstrak metanol Sargassum sp.  mengandung pigmen klorofil a sebesar 2,84 mg/g, klorofil b sebesar 1,15 mg/g, dan karotenoid sebesar 2,63 μmol/g. Kadar total fenolik ekstrak metanol adalah 57,97 mgGAE/g, sementara aktivitas antioksidannya  (IC50) sebesar 69,27 ppm.  Berdasarkan nilai IC50nya, ekstrak metanol Sargassum sp berpotensi sebagai antioksidan kuat (kurang dari 100 ppm).

scholarly journals Aktivitas Antioksidan Ekstrak Daun Mangrove Rhizopora mucronata

2017 ◽  
Vol 6 (2) ◽  
pp. 110 ◽  
Author(s):  
Ali Ridlo ◽  
Rini Pramesti ◽  
Koesoemadji Koesoemadji ◽  
Endang Supriyantini ◽  
Nirwani Soenardjo
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Chlorophyll A ◽  
Inhibitory Concentration ◽  
Methanol Extract ◽  
Ethyl Acetate Extract ◽  
Hexane Extract ◽  
Carotenoid Content ◽  
Total Phenolic ◽  
Ic50 Value

Rhizopora mucronata merupakan salah satu jenis mangrove yang berpotensi  sebagai sumber antioksidan alami. Daun tanaman ini mengandung senyawa metabolit sekunder seperti tanin, fenolat, klorofil, karotenoid dan alkaloid. Penelitian ini bertujuan menentukan aktivitas antioksidan ekstrak daun R. mucronata. Sampel diambil dari kawasan mangrove Tugurejo, Semarang dan diekstraksi  secara bertingkat berturut-turut dengan pelarut n-heksana, etil asetat dan metanol. Aktivitas antioksidan diukur dengan metode DPPH (1,1-diphenyl-2-picrylhidrazyl) dan nilainya ditentukan berdasarkan nilai Inhibitory Concentration (IC50)pada panjang gelombang 516,5 nm. Kadar senyawa fenolat total ditentukan secara spektrofotometri pada panjang gelombang 725 nm dengan metode Folin-Ciocalteu, kadar klorofil a dan b ditentukan dengan metode spektrofotometri pada panjang gelombang 663 nm dan 645 nm dan kadar karotenoid diukur pada 480 nm. Hasil penelitian menunjukkan ekstrak metanol memiliki nilai IC50terkecil (113,41  ppm), diikuti ekstrak n-heksana 151,13 ppm dan ekstrak etil asetat 184,78 ppm. Kandungan total fenolat tertinggi terdapat pada ekstrak metanol yaitu 21,06 mg GAE/g sampel, ekstrak n-heksana 13,27 mg GAE/g sampel dan ekstrak etil asetat 2,08 mg GAE/g sampel. Kandungan klorofil a tertinggi terdapat pada ekstrak metanol yaitu 2,304  mg/g, diikuti ekstrak n-heksana 0,705 mg/g dan ekstrak etil asetat 0,64 mg/g. Kandungan klorofil b tertinggi dicapai ekstrak metanol yaitu 0,97 mg/g, ekstrak n-heksana 0,50 mgg dan ekstrak etil asetat 0,13 mg/g. Kandungan karotenoid tertinggi dicapai pada ekstrak metanol yaitu 6,49 mg GAE/g, diikuti ekstrak etil asetat (0.54 mg GAE/g) dan ekstrak n-heksana (1,37 mg GAE/g). Ekstrak metanol memiliki aktivitas antioksidan tertinggi dan termasuk dalam antioksidan kategori sedang, sedangkan ekstrak etil asetat dan  n-heksana termasuk dalam  antioksidan kategori lemah.   Rhizopora mucronata is one type of mangrove that has the potential as a source of natural antioxidants. The leaves of this plant contain secondary metabolite compounds such as tannins, phenolics, chlorophyll, carotenoids and alkaloids. This study aims to determine the antioxidant activity of R. mucronata leaf extract. Samples were taken from Tugurejo mangrove area, Semarang and extracted successively with n-hexane, ethyl acetate and methanol solvent. Antioxidant activity was measured by DPPH (1,1-diphenyl-2-picrylhidrazyl) method and its value was determined based on Inhibitory Concentration (IC50) value at 516,5 nm wavelength. Total phenolic compound concentration was determined spectrophotometrically at 725 nm wavelength with Folin-Ciocalteu method. The levels of chlorophyll a and b was determined by spectrofotometry method at 663 nm and 645 nm wavelength and carotenoid levels were measured at 480 nm. The results showed that methanol extract had the smallest IC50 value (113,41 ppm), followed by n-hexane extract 151,13 ppm and ethyl acetate extract 184,78 ppm. The highest total phenolic content was found in methanol extract, 21.06 mg GAE / g sample, n-hexane extract 13,27 mg GAE / g sample and ethyl acetate extract 2.08 mg GAE / g sample. The highest content of chlorophyll a contained in methanol extract is 2,304 mg / g, followed by n-hexane extract 0,705 mg / g and ethyl acetate extract 0,64 mg / g. The highest content of chlorophyll b was methanol extract of 0.97 mg / g, n-hexane extract 0,50 mgg and ethyl acetate extract 0,13 mg / g. The highest carotenoid content was achieved in the methanol extract of 6.49 mg GAE / g, followed by ethyl acetate extract (0.54 mg GAE / g) and n-hexane extract (1.37 mg GAE / g). Methanol extract has the highest antioxidant activity and is included in medium category antioxidants, while ethyl acetate and n-hexane extracts are included in weak category antioxidants. 

scholarly journals Effects of solvent extraction system on antioxidant activity of Lamium purpureum L.

2017 ◽  
Vol 71 (5) ◽  
pp. 361-370
Author(s):  
Slavica Grujic ◽  
Ana Dzamic ◽  
Violeta Mitic ◽  
Vesna Stankov-Jovanovic ◽  
P.D. Marin ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Radical Scavenger ◽  
Scavenging Activity

Antioxidant and free radical scavenging activity of methanol, ethanol, ethyl acetate and chloroform extracts of aerial parts of Lamium purpureum L. was determined by DPPH, ABTS, FRAP and TRP assays. Contents of flavonoids and phenols were also investigated. The total phenolic content in the extracts, determined using Folin?Ciocalteu assay, ranged between 8.57 to 128.00 mg GAE/g d.e. while concentrations of flavonoids in the extracts varied from 24.20 to 39.80 mg QuE/g d.e. The highest phenolic content was found in methanol extract (128.00 mg GAE/g d.e.). The highest content of total flavonoids was identified in the methanol extract (39.80 mg QuE/g d.e.) and the lowest was in the chloroform (24.30 mg QuE/g d.e.). DPPH scavenging of the extracts was determined and obtained IC50 values ranged from 0.12 to 3.12 mg/mL of solution. The values of ABTS radical scavenging activity ranged from 0.35 to 1.80 mg AA/g. The highest ABTS antiradical activity was registered for methanol extract. The FRAP value was found within the range 0.08 to 1.04 ?mol Fe/mg. The best radical scavenger was methanol (1.04 ?mol Fe/mg). In reducing power assay different extracts of L. purpureum showed increasing of activity with increased concentration, and all extracts possessed substantial dose dependent antioxidant activity. The best reducing capacity was obtained with methanol extract of L. purpureum (0.0132 mg AA/mL). The results in this study confirmed that L. purpureum possesses moderate antioxidant properties.

scholarly journals Vachellia farnesiana (L.) Wight & Arn. Growing in Bangladesh Exerts In-vitro Antioxidant and In-vivo Analgesic and Anti-diarrheal Activities

2020 ◽  
Vol 23 (2) ◽  
pp. 181-186
Author(s):  
Md Sadman Hasib ◽  
Md Sazzadul Bari ◽  
Akhteruzzaman Chowdhury ◽  
Md Aslam Hossain ◽  
Mohammad A Rashid
Keyword(s):  
Body Weight ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Tail Flick ◽  
Vitro Assay ◽  
Ic50 Value ◽  
Analgesic Activities

The present study was conducted to evaluate the antioxidant, antidiarrheal and analgesic activities of Vachellia farnesiana (L.) Wight & Arn. The methanol extract of V. farnesiana and its different fractionates were subjected to in-vitro assay for the determination of total phenolic content and antioxidative potential. The ethyl acetate soluble fraction (EASF) exhibited the highest free radical scavenging capacity (IC50 value of 21.49 ± 1.04 μg/ml) as compared to that exhibited by the standard butylated hydroxyl toluene (BHT) (IC50 value of 20.41 ± 0.05 μg/ml). Such prominent antioxidative potential was further reinforced by a phenolic content of 39.26 ± 0.85 mg of gallic acid equivalent per gram of extract. The plant extract, at the dose of 400 mg/kg body weight, reduced castor oil-induced diarrhea in mice model by a statistically significant (p < 0.05) margin of 47.62%, while the standard loperamide produced 66.67% reduction of diarrheal feces. The central and peripheral analgesic activities of the crude methanol extract of V. farnesiana (MEVF) was determined by tail flick- and acetic acidinduced writhing methods, respectively, in Swiss albino mice. In the tail flick method, oral administration of MEVF at doses of 200 and 400 mg/kg body weight exhibited 221.09 and 237.09% elongation of pain response time, respectively, after 90 minutes of administration whereas the standard morphine effectuated 518.34% elongation within the same time. Furthermore, the same doses of the extract illustrated 63.27 and 69.39% reductions, respectively, in the acetic acid-induced abdominal constrictions in mice. Compared to the standard acetylsalicylic acid with 75.51% inhibition, statistically significant (p < 0.05) peripheral analgesic activity was established. The results of the present investigations suggest that methanol extract of V. farnesiana possesses antioxidant, antidiarrheal and analgesic activities which eventually indicates the presence of biologically important phytoconstituents within the plant that needs further exploration. Bangladesh Pharmaceutical Journal 23(2): 181-186, 2020

scholarly journals Antimicrobial and antioxidant activity of Epimedium pinnatum

2013 ◽  
Vol 59 (2) ◽  
pp. 24-34 ◽  
Author(s):  
Mohaddese Mahboubi ◽  
Nastaran Kazempour ◽  
Hossein Hosseini ◽  
Mona Mahboubi
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Aqueous Extract ◽  
Methanol Extract ◽  
Ethanolic Extract ◽  
Total Phenolic ◽  
Spectrophotometric Methods ◽  
Ic50 Value ◽  
Antioxidant And Antimicrobial Activity ◽  
Antimicrobial And Antioxidant Activity

Summary Epimedium pinnatum (Berberidaceae family) is used as an aphrodisiac in traditional Chinese medicine (TCM). The aim of this study was to evaluate the antimicrobial and antioxidant activity of E. pinnatum extracts (ethanol, methanol and aqueous extracts). Total phenolic (TPC) and flavonoid contents (TFC) of each extract were assessed by spectrophotometric methods. It was exhibited that methanol extract had better antimicrobial activity than those of ethanolic extract or aqueous extract. The TPC and TFC of E. pinnatum extracts was higher in methanol extract (149 and 36.6 mg/g) than that of ethanolic extract (137.2 and 19.5 mg/g) and aqueous extract (86.2 and 8.4 mg/g). The methanol extract had lower IC50 value (200 µg/ml) than ethanolic (250 µg/ml) and aqueous extract (400 µg/ml). There was a positive correlation between TPC, TFC in E. pinnatum extract and their antioxidant and antimicrobial activity

scholarly journals Uji Aktivitas Antioksidan dan Fenolik Total Fraksi Etil Asetat Daun Sembung (Blumea balsamifera (L.) DC)

2021 ◽  
Vol 12 (2) ◽  
Author(s):  
Azimatur Rahmi ◽  
Tika Afriani ◽  
Linda Hevira ◽  
Wike Widiawati
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethyl Acetate Fraction ◽  
Total Phenolic ◽  
Activity Assay ◽  
Ic50 Value

The aim of this study is to investigate antioxidant activity and total phenolic content of ethyl acetate fraction of Sembung leaves. Sembung (Blumea balsamifera L.) is a plant that has the potential as an antioxidant because it contains secondary metabolites of alkaloids, steroids, flavonoids, saponins, and phenolic compounds. In this study, antioxidant activity was tested by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) for free radical scavenging activity assay. Evaluation of total phenolic content was tested by using the Folin-Ciocalteau method. The results showed that the ethyl acetate fraction of Sembung leaves possess showed medium antioxidant activity with IC50 valueof 221.821 compare with reference standard ascorbic acid with IC50 value 68.25 ppm. The ethyl acetate fraction of Sembung leaves has a total phenolic content of 161.101 mg GAE/g. This study provided that Blumea balsamifera leaves possess antioxidant.

scholarly journals STUDI OPTIMASI EKSTRAKSI KANDUNGAN SENYAWA FENOLIK TOTAL DAN UJI AKTIVITAS ANTIOKSIDAN DARI DAUN MANGGIS (Garcinia mangostana Linn.)

2019 ◽  
Vol 10 (1) ◽  
pp. 36
Author(s):  
Emil Salim ◽  
Yogi Afritunando ◽  
Nindi Antika Febriana ◽  
Mai Efdi
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Total Phenolic Compounds ◽  
Garcinia Mangostana ◽  
Activity Test ◽  
Ic50 Value ◽  
Dpph Method

This study aims to determine antioxidant activity and total phenolic content of mangosteen leaves in several different solvents. The antioxidant activity test of the extract was determined by the DPPH method and the total phenolic content was determined by the Folin-Ciocalteu method. The phytochemical test of mangosteen leaves showed the presence of phenolic compounds, saponins, triterpenoids and alkaloids. The extraction results increase with increasing water content in methanol, ethanol and acetone. The highest amount of extract was obtained using 50% acetone solvent (34.98 ± 0.17%). Extraction using 100% ethanol showed the highest total phenolic content (614.25 ± 5.58 mg GAE / g extract) and the lowest IC50 value (5.81 mg / L). The results of this study indicate that the use of 50% acetone solvent can optimize the amount of extract and 100% ethanol can optimize the content of total phenolic compounds and antioxidant activity of mangosteen leaves.

Estimation of total Phenolic and ϑlavonoids content and Studies on antioxidant activity of different extracts of Olax scandens by FRAP assay

2020 ◽  
Vol 11 (3) ◽  
pp. 3367-3371
Author(s):  
Pranaya P ◽  
Akiladevi D
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Frap Assay ◽  
Aerial Parts ◽  
Reducing Ability ◽  
Ic50 Value

Olax scandens Roxb. (family Olacaceae) available in throughout tropical India. The current study, aerial parts of different concentrates of Olax scandens was evaluated for its in-vitro antioxidant potential by FRAP assay taking ascorbic acid as the standard and estimation of total phenolic content and flavonoids content. The IC50 value was originated that methanolic concentrates of Olax scandens are more efficient in antioxidant activity by FRAP methods compared EA & PE concentrates. The methanolic concentrates of Olax scandens & ascorbate exhibited antioxidant potential possessing IC50 207µg/ml & 50µg/ml by Ferric reducing ability Power assay. The methanolic and EA concentrates of Olax scandens showed the total phenolic content (14.426 ± 0.032, 4.128 ± 0.025) respectively, and ৒lavonoids content (11.526 ± 0.054, 3.682 ± 0.042) respectively. Invitro antioxidant studies show methanolic concentrates of Olax scandens have better antioxidant activity as well as a higher content of total phenolic and flavonoids content. These results indicate that aerial parts of methanolic concentrates Olax scandens could serve as a natural antioxidant, which may be useful in preventing free radical-induced diseases.

Chemical Composition of Essential Oil and in Vitro Biological Activities of Dryopteris marginalis L.

2019 ◽  
Vol 16 ◽  
Author(s):  
Md. Zahidul Islam ◽  
M. Shahinuzzaman ◽  
Raşit Fikret Yilmaz ◽  
Md. Hafizur Rahman ◽  
Yavuz Derin ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Superoxide Radical ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Organic Extracts ◽  
Radical Scavenging Assay

Dryopteris marginalis L. is a local plant in Bangladesh which is widely used as folk medicine. In this study, the chemical composition of essential oil of D. marginalis L. as well as the antioxidant activity, antibacterial activity and total phenolic content of various organic extracts were analyzed. The gas chromatography–mass spectrometry analysis was used to identify the chemical compositions. The antibacterial activity of different extracts of Dryopteris marginalis was examined against three gram-negative bacteria and two gram-positive bacteria. The antibacterial activity of different extracts was investigated using disc diffusion method and minimum inhibitory concentration showing important antibacterial activity and was measured by using broth dilution method. The antioxidant activity was determined by using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay and superoxide radical scavenging assay. The total phenolic portion of the extracts were ascertained by Folin-Ciocalteu in alkaline medium. Twenty-two volatile compounds were identified from the essential oil. The methanol extract exhibited significant inhibitory effect which was close to the value of standard antibiotic (Streptomycin). The zones of inhibition of different organic extracts against the tested bacteria were found in the range of (7-15) mm and the minimum inhibitory concentrations (MIC) were recorded (15.62-1000) µg/ml. The antioxidant activity of D. marginalis was (82.13+1.2) % and (59.21+0.45) % highest activity with DPPH and superoxide radical scavenging assay respectively. The methanol extract showed highest (147.43+3.1) mg/g GAE of total phenolic content. The extracts and essential oil of D. marginalis showing strong antibacterial activity can be considered as a potential source of natural medicine and the ingredient of modern therapeutic agents.

scholarly journals Assessment of Total Phenolic, Flavonoid Content and Antioxidant Activity of Ocimum sanctum Linn

2019 ◽  
Vol 40 ◽  
pp. 30-35
Author(s):  
Ishwor Pathak ◽  
Muna Niraula
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Colorimetric Method ◽  
Radical Scavenging ◽  
Hexane Extract ◽  
Ocimum Sanctum ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Ic50 Value ◽  
Phenolic And Flavonoid

Ocimum sanctum, commonly known as Tulasi in Nepal, is a pharmacologically important plant due to its active constituents. In this work, extraction was carried out in hexane, chloroform and methanol solvents and their phytochemical screening was performed. Total phenolic and flavonoid contents in the plant were measured by Folin-Ciocalteu colorimetric method and aluminum chloride colorimetric method respectively. Antioxidant activity of the extracts was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay. Alkaloids, flavonoids, tannins, glycosides, polyphenols, terpenoids, tannins and steroids are mainly found in the extracts. Based on the result obtained, the plant posses a significant amount of total phenolic and total flavonoid content. Both phenolic and flavonoid contents were highest in methanol extract, followed by chloroform and hexane extract. Antioxidant activity of the extracts as ascorbic acid standard (IC50 value = 41.34 µg/mL) was in the order of methanol extract (IC50 value = 47.73 µg/mL) > chloroform extract (IC50 value = 79.46 µg/mL) > hexane extract (IC50 value = 94.68 µg/mL). The extent of the antioxidant activity of the plant is following the number of total phenolics and flavonoids present in it.

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