scholarly journals Production of xylanase by Fusarium oxysporum using agro-industrial waste

2021 ◽  
Vol 17 (8) ◽  
Author(s):  
Lunara Thais Alves de Bastos ◽  
Ryhára Dias Batista ◽  
Alanna Cristinne Martins Lima ◽  
Iara Leandro dos Santos ◽  
Fabrício Coutinho de Paula-Elias ◽  
...  

This study aimed to optimize the production of xylanase, under submerged conditions using Fusarium oxysporum. Firstly, it was carried out the selection of the best source of carbon from agricultural waste, such as soybean husk, cassava peel, pineapple crown, corn straw, rice husk, bacaba, barley bagasse and, corncob. They were milled and sieved with a maximum granulation of 1 mm. The cultures were carried out by 132 hours 30 °C and 180 rpm. Among the agroindustrial residues, corn straw was the one that stood out with a greater enzymatic activity of 12.6 U/mL. In the second step, it was used Plackett-Burman design to screen the nutrients sources important to xylanase production. Thus, independent variables significant were urea and MgSO4. These variables selected by Plackett-Burman were then used in a Central Composite Rotational Design present activity of 26.6 U/mL and the predicted was 34.5 U/mL. So, the xylanase production by F. oxysporum can be optimize using corn straw, a low-cost waste found in large quantities.

2017 ◽  
Vol 42 (3) ◽  
Author(s):  
Ramesh Bandikari ◽  
Umamahesh Katike ◽  
Naga Sivudu Seelam ◽  
Vijaya Sarathi Reddy Obulam

AbstractAimEvaluation of the medium components and conditions for the optimization of xylanase production in submerged fermentation byMethodswas identified by the 18s rRNA. NaOH pre-treated corn cobs were used in order to reduce the crystallinity of corn cobs. De-oiled cakes proximate composition was analyzed according to AOAC. Plackett-Burman design (PBD) was used to screen the 19 media variables that affect xylanase production and optimized the media by central composite design (CCD).ResultsThe isolate was identified asConclusionNaOH pre-treated corn cobs were used with mustard cake powder as fermentation medium constituent to induce the xylanase production. From the results we conclude that mustard cake induced the xylanase production.


1998 ◽  
Vol 19 (1) ◽  
pp. 61-65 ◽  
Author(s):  
S. Krishnan ◽  
S. G. Prapulla ◽  
D. Rajalakshmi ◽  
M. C. Misra ◽  
N. G. Karanth

Author(s):  
Fikriye Alev Akçay ◽  
Ayşe Avcı

In this study, effects of medium components and inoculum size on the protease production by Bacillus sp. EBTA6 that was isolated from a home-made Tarhana sample were investigated. The cell-free supernatant of bacterium cultured on a shaking incubator for 24 h was used to test protease activity as the response. With a total number of 11 factors, 12 different experiments were run and the highest experimental protease activity was measured as 2280.4 U/mL. Results were analyzed statistically by ANOVA and the most efficient factors were detected as yeast extract, dipotassium phosphate, casein, and peptone with a contribution of 93.78, 2.19, 1.96, 1.31%, respectively. For validation of the selected factors, a further experiment was performed by using of yeast extract (9.98 g/L), dipotassium phosphate (1.27 g/L), casein (8.69 g/L), and peptone (9.88 g/L) obtained from the design equation. The experimental response was found as 2411.4 U/mL which was only 5.5% higher than the predicted response showing that the model was applicable.


2017 ◽  
Vol 23 (2) ◽  
pp. 237-244
Author(s):  
Zahra Mokhtari-Hosseini ◽  
Razieh Hosseinabadi ◽  
Ashrafalsadat Zarmi

Disposal of storage tank sludge in oil depots is a major environmental concern due to the high concentration of hydrocarbons involved. This paper investigates the reduction of the sludge oil pollutants with initial oil and grease concentration of about 50 mass% using low cost adsorbents. Among the examined adsorbents, sawdust indicated the maximum removal of oil and grease. The screening and optimizing of process parameters were evaluated employing Plackett-Burman design and response surface method. For the optimized conditions, more than 60 mass% of oil and grease from the sludge was removed. Moreover, it was found that sawdust adsorption of the oil and grease approximately followed the Freundlich isotherm. The results indicated that oil pollutants of sludge could be reduced using sawdust as a low-cost, available and flammable adsorbent so that thus saturated adsorbents could be used as fuel in certain industries.


2012 ◽  
Vol 730-732 ◽  
pp. 648-652 ◽  
Author(s):  
Joana Carvalho ◽  
André Ribeiro ◽  
Jorge Araújo ◽  
Fernando Castro

As the current global trend towards more stringent environmental standards, technical applicability and cost-effectiveness became key factors in the selection of adsorbents for water and wastewater treatment. Recently, various low-cost adsorbents derived from agricultural waste, industrial by-products or natural materials, have been intensively investigated. In this respect, the eggshells from egg-breaking operations constitute significant waste disposal problems for the food industry, so the development of value-added by-products from this waste is to be welcomed. The egg processing industry is very competitive, with low profit margins due to global competition and cheap imports. Additionally, the costs associated with the egg shell disposal (mainly on landfill sites) are significant, and expected to continue increasing as landfill taxes increase. The aim of the present review is to provide an overview on the development of low-cost adsorbents derived from eggshell by-products.


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