Body temperature and cold sensation during and following exercise under temperate room conditions in cold-sensitive young trained females

Teeth are composed of many tissues, covered by an inflexible and obdurate enamel. Unlike most other tissues, teeth become extremely cold sensitive when inflamed. The mechanisms of this cold sensation are not understood. Here, we clarify the molecular and cellular components of the dental cold sensing system and show that sensory transduction of cold stimuli in teeth requires odontoblasts. TRPC5 is a cold sensor in healthy teeth and, with TRPA1, is sufficient for cold sensing. The odontoblast appears as the direct site of TRPC5 cold transduction and provides a mechanism for prolonged cold sensing via TRPC5’s relative sensitivity to intracellular calcium and lack of desensitization. Our data provide concrete functional evidence that equipping odontoblasts with the cold-sensor TRPC5 expands traditional odontoblast functions and renders it a previously unknown integral cellular component of the dental cold sensing system.

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Whole-Genome Resequencing Points to Candidate DNA Loci Affecting Body Temperature under Cold Stress in Siberian Cattle Populations

Life ◽

10.3390/life11090959 ◽

2021 ◽

Vol 11 (9) ◽

pp. 959

Author(s):

Alexander Igoshin ◽

Nikolay Yudin ◽

Ruslan Aitnazarov ◽

Andrey A. Yurchenko ◽

Denis M. Larkin

Keyword(s):

Body Temperature ◽

Cold Stress ◽

Candidate Snps ◽

Whole Genome ◽

Genome Resequencing ◽

Functional Annotations ◽

Cold Environments ◽

Cold Tolerant ◽

Cold Sensitive ◽

Whole Genome Resequencing

Despite the economic importance of creating cold resilient cattle breeds, our knowledge of the genetic basis of adaptation to cold environments in cattle is still scarce compared to information on other economically important traits. Herein, using whole-genome resequencing of animals showing contrasting phenotypes on temperature maintenance under acute cold stress combined with the existing SNP (single nucleotide polymorphism) functional annotations, we report chromosomal regions and candidate SNPs controlling body temperature in the Siberian cattle populations. The SNP ranking procedure based on regional FST calculations, functional annotations, and the allele frequency difference between cold-tolerant and cold-sensitive groups of animals pointed to multiple candidate genes. Among these, GRIA4, COX17, MAATS1, UPK1B, IFNGR1, DDX23, PPT1, THBS1, CCL5, ATF1, PLA1A, PRKAG1, and NR1I2 were previously related to thermal adaptations in cattle. Other genes, for example KMT2D and SNRPA1, are known to be related to thermogenesis in mice and cold adaptation in common carp, respectively. This work could be useful for cattle breeding strategies in countries with harsh climates, including the Russian Federation.

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Effects of testosterone, estradiol, and temperature on neurons in preoptic tissue slices

AJP Regulatory Integrative and Comparative Physiology ◽

10.1152/ajpregu.1986.250.4.r625 ◽

1986 ◽

Vol 250 (4) ◽

pp. R625-R632 ◽

Cited By ~ 12

Author(s):

N. L. Silva ◽

J. A. Boulant

Keyword(s):

Body Temperature ◽

Firing Rate ◽

Reproductive Systems ◽

Tissue Slices ◽

Cold Sensitive ◽

Steroid Sensitive ◽

Reproductive Steroids

Thermosensitive preoptic neurons have been implicated in the regulation of body temperature. Testosterone- and estrogen-sensitive preoptic neurons have been implicated in reproductive behavioral and endocrine responses. In this study, rat preoptic tissue slices were used to examine the specificity of these neurons by determining their individual firing rate responses to both temperature and reproductive steroids. Of the 180 neurons classified according to thermosensitivity, 37% were warm sensitive, 8% were cold sensitive, and 55% were temperature insensitive. Ninety-three neurons were tested for their responses to perfusion media containing either testosterone or estradiol (30 pg/ml). Of the cells tested with both steroids, testosterone or estradiol affected half of the thermosensitive neurons and one-third of the temperature-insensitive neurons. This indicates that the population of temperature-insensitive neurons does not contain the majority of the steroid-sensitive neurons. There was much specificity, however, between the two types of steroid-sensitive neurons; testosterone and estradiol rarely affected the same neuron. Although these findings do not indicate a strong specificity between thermosensitive and steroid-sensitive neurons, they do support previous studies suggesting interactions between thermoregulatory and reproductive systems.

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Hyperthermic Effect of Ginger (Zingiber officinale) Extract-Containing Beverage on Peripheral Skin Surface Temperature in Women

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2018/3207623 ◽

2018 ◽

Vol 2018 ◽

pp. 1-8

Author(s):

Keiichiro Sugimoto ◽

Hiroaki Takeuchi ◽

Kazuya Nakagawa ◽

Yasuhiro Matsuoka

Keyword(s):

Body Temperature ◽

Crossover Trial ◽

Zingiber Officinale ◽

Skin Surface ◽

The Body ◽

Cold Sensitivity ◽

Skin Surface Temperature ◽

Ginger Extract ◽

Thermographic Camera ◽

Cold Sensitive

Ginger is known to warm the body. Therefore, we conducted a placebo-controlled crossover trial to investigate the hyperthermic effect of a palatable ginger-containing beverage in healthy women with cold-sensitive extremities. Six women drank 280 mL of 0.07% ginger extract-containing or placebo beverage in a temperature-controlled room (21°C). Their palm temperatures were measured as measure of surface body temperature using a thermographic camera before intake and every 10 min after intake for 60 min. Palm temperature increased immediately following intake of the ginger and placebo beverages. However, palm temperature following intake of the ginger beverage increased for 20 min, while palm temperature following placebo intake decreased again after 10 min. The increased palm temperature following ginger intake was maintained significantly longer than after placebo intake (p < 0.05). In response to a questionnaire, some subjects answered that their increased body temperature was maintained after drinking the ginger beverage. Ginger extract-containing beverage may thus improve cold sensitivity.

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Selective blockade of TRPA1 channel attenuates pathological pain without altering noxious cold sensation or body temperature regulation

Pain ◽

10.1016/j.pain.2011.01.049 ◽

2011 ◽

Vol 152 (5) ◽

pp. 1165-1172 ◽

Cited By ~ 174

Author(s):

Jun Chen ◽

Shailen K. Joshi ◽

Stanley DiDomenico ◽

Richard J. Perner ◽

Joe P. Mikusa ◽

...

Keyword(s):

Body Temperature ◽

Temperature Regulation ◽

Body Temperature Regulation ◽

Cold Sensation ◽

Selective Blockade ◽

Pathological Pain ◽

Noxious Cold

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Temperature sensitivity of neurons in slices of the rat PO/AH area: effect of bombesin and substance P

AJP Regulatory Integrative and Comparative Physiology ◽

10.1152/ajpregu.1993.264.2.r449 ◽

1993 ◽

Vol 264 (2) ◽

pp. R449-R455 ◽

Cited By ~ 1

Author(s):

H. A. Schmid ◽

L. Jansky ◽

F. K. Pierau

Keyword(s):

Body Temperature ◽

Substance P ◽

Temperature Sensitivity ◽

Opposite Effect ◽

Temperature Sensitive ◽

Hypothalamic Area ◽

Physiological Processes ◽

Cold Sensitive ◽

Neurophysiological Basis

The effects of bombesin (Bom) and substance P (SP) were investigated in 156 temperature-sensitive and -insensitive neurons in slices of the preoptic and anterior hypothalamic area (PO/AH) of rats. Application of Bom increased the firing rate (FR) in 68% (n = 38) of the warm-sensitive and in 62% (n = 39) of the temperature-insensitive neurons. One cold-sensitive neuron was excited; a second was not affected by the peptide. No neuron decreased its activity after Bom application. SP excited 80% (n = 15) of the warm-sensitive neurons and 48% (n = 29) of the temperature-insensitive neurons. Two cold-sensitive neurons were inhibited by SP, a third one was not affected. The opposite effect on thermoregulation in vivo caused by the two peptides cannot be explained simply by their relatively similar excitatory effects on the FR of PO/AH neurons. After Bom application the temperature coefficient (TC) was significantly elevated in 7 out of 11 warm-sensitive neurons and in 19 out of 21 temperature-insensitive neurons. After SP application the TC was significantly reduced in 6 out of 7 warm-sensitive and 1 out of 12 temperature-insensitive neurons. Bom caused grouped discharges (bursts) in 7 out of 42 PO/AH neurons; SP never produced bursts in the discharge pattern. The increase of the TC of warm-sensitive and the transformation of temperature-insensitive into warm-sensitive neurons by Bom might be regarded as the neurophysiological basis for the decreased body temperature after Bom application. It is concluded that the temperature sensitivity of PO/AH neurons is not an unchangeable inherent property of certain cells but may be altered or even evoked by physiological processes like the release of neuromodulators.

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Scanning Electron Microscopy and X-Ray Analyses of Dental Tissues from a Hibernator

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100090877 ◽

1976 ◽

Vol 34 ◽

pp. 178-179

Author(s):

M. L. Zimny ◽

A. C. Haller

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Body Temperature ◽

Ground Squirrel ◽

Alveolar Bone ◽

X Ray ◽

Dental Tissues ◽

Bone Minerals ◽

Apical Tooth ◽

Scanning Electron

During hibernation the ground squirrel is immobile, body temperature reduced and metabolism depressed. Hibernation has been shown to affect dental tissues varying degrees, although not much work has been done in this area. In limited studies, it has been shown that hibernation results in (1) mobilization of bone minerals; (2) deficient dentinogenesis and degeneration of alveolar bone; (3) presence of calculus and tears in the cementum; and (4) aggrevation of caries and pulpal and apical tooth abscesses. The purpose of this investigation was to study the effects of hibernation on dental tissues employing scanning electron microscopy (SEM) and related x-ray analyses.

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