Short‐term hypoxic training increases monocarboxylate transporter 4 and phosphofructokinase activity in Thoroughbreds

Background: Colorectal cancer (CRC) is currently the third most common cancer type in males and the second most occurring in females. The role of microRNA (miRNA) in the development of colorectal cancer is not fully elucidated. Therefore, understanding the mechanistic interaction between miRNA and their target oncogenes may hold great importance as a possible target for interventional anticancer therapy. Aims: To identify miRNAs that are part of the regulating pathway of Monocarboxylate Transporter-4 (MCT4) and Vascular Endothelial Growth Factor (VEGF) oncogenes. Study Design: We used publicly available prediction tools (e.g. TargetScan, MicroCosm, PicTar, and DIANA-microT-CDS) to identify the possible miRNA that target the two oncogenes. Methodology: We used the GeneMania database to visualize the network and verify gene names and remove ambiguity and duplications. Furthermore, we used miRTarBase database to identify experimentally validated targets which we used to further confirm miRNA-oncogene relationships. Finally, we utilized miR-Mfold web-tool to further visualize the circular structures and the simulated miR-1 and miR-206 targeting arrangements. Results: We found two putative miRNA (miR-1 and miR-206) that may downregulate MCT4 coded by SLC16A3 gene and VEGF which is coded by VEGF gene. We found relationships between the validated target genes of miR-1 and miR-206 through GeneMania which we extracted from the literature. And we elucidated the proposed structure of these two miRNAs through miR-Mfold web-tool. Conclusion: Our results elucidated a novel regulation pathway in CRC cells and may suggest a potential therapeutic approach for CRC therapy. MiR-1 and miR-206 may help cells go to apoptosis and inhibit the angiogenesis of colorectal cancer cells by down-regulation of MCT4 and VEGF proteins in tumor tissues.

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INFLUENCES OF SHORT-TERM NORMOBARIC HYPOXIC TRAINING ON METABOLIC SYNDROME-RELATED MARKERS IN OVERWEIGHT AND NORMAL-WEIGHT MEN

Journal of Men s Health ◽

10.22374/1875-6859.14.1.5 ◽

2018 ◽

Vol 14 (1) ◽

pp. e44-e52

Author(s):

Sohee Shin ◽

Toshio Matsuoka ◽

Wi-Young So

Keyword(s):

Metabolic Syndrome ◽

Heart Rate ◽

Density Lipoprotein ◽

Exercise Group ◽

Normal Weight ◽

Body Fat Percentage ◽

Fat Percentage ◽

Short Term ◽

Hypoxic Training ◽

Weight Groups

Background and Objective This study examined the influence of short-term normobaric hypoxic training on metabolic syndrome-related markers in overweight and normal-weight men. Material and Methods Forty-one Japanese men were included and were divided into two groups based on their body mass indices (BMIs): BMI≥25 or BMI<25. Participants in the overweight and normal-weight groups were randomly classified into the hypoxic exercise group (hypoxic overweight, HO; hypoxic normal-weight, HN) and the normoxic exercise group (normoxic overweight, NO; normoxic normal-weight, NN). Subjects performed treadmill exercise three days per week for four weeks at an exercise intensity of 60% of maximum heart rate, under either normobaric hypoxic or normobaric normoxic conditions, for 50 min (including 5 min warm-up and cool-down periods) after a 30-min rest period. The study parameters included weight, body fat percentage, BMI, heart rate, waist circumference, ankle-brachial pulse wave velocity (PWV), blood sugar, triglycerides (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), fasting insulin, Homeostasis Model Assessment of Insulin Resistance (HOMA-IR) scores, and adiponectin levels. Repeated measures two-way analysis of variance was used to examine differences in the mean parameter values between the two groups (overweight and normal-weight) before and after training. Results Hypoxic training improved the weight, body fat percentage, BMI, waist circumference, PWV, TC, LDL-C levels, and HOMA-IR scores in the overweight and normal-weight groups (p<0.05). In addition, TG level, HDL-C level, and HOMA-IR scores showed significant interactions with hypoxic training, as these parameters improved in the hypoxic overweight group (p<0.05). Conclusion These results suggest that hypoxic training could be useful for improving arterial stiffness, circulatory system function, body composition, and energy metabolism in adult males.

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Effects of streptozotocin-induced diabetes on markers of skeletal muscle metabolism and monocarboxylate transporter 1 to monocarboxylate transporter 4 transporters

Metabolism ◽

10.1053/meta.2002.33343 ◽

2002 ◽

Vol 51 (7) ◽

pp. 807-813 ◽

Cited By ~ 22

Author(s):

Guillaume Py ◽

Karen Lambert ◽

Ollivier Milhavet ◽

Nicolas Eydoux ◽

Christian Pr[eacute]faut ◽

...

Keyword(s):

Skeletal Muscle ◽

Muscle Metabolism ◽

Monocarboxylate Transporter ◽

Skeletal Muscle Metabolism ◽

Monocarboxylate Transporter 1 ◽

Streptozotocin Induced Diabetes ◽

Monocarboxylate Transporter 4

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Monocarboxylate Transporter 4 Is a Therapeutic Target in Non-small Cell Lung Cancer with Aerobic Glycolysis Preference

Molecular Therapy — Oncolytics ◽

10.1016/j.omto.2020.06.012 ◽

2020 ◽

Vol 18 ◽

pp. 189-201 ◽

Cited By ~ 1

Author(s):

Ting-Chun Kuo ◽

Kuo-Yen Huang ◽

Shuenn-Chen Yang ◽

Sean Wu ◽

Wei-Chia Chung ◽

...

Keyword(s):

Lung Cancer ◽

Small Cell Lung Cancer ◽

Cell Lung Cancer ◽

Therapeutic Target ◽

Aerobic Glycolysis ◽

Small Cell ◽

Monocarboxylate Transporter ◽

Small Cell Lung ◽

Monocarboxylate Transporter 4

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Intratumoral reciprocal expression of monocarboxylate transporter 4 and glypican-3 in hepatocellular carcinomas

BMC Research Notes ◽

10.1186/s13104-019-4778-y ◽

2019 ◽

Vol 12 (1) ◽

Cited By ~ 1

Author(s):

Kenji Yorita ◽

Akinobu Ohno ◽

Takahiro Nishida ◽

Kazuhiro Kondo ◽

Toshihiko Ohtomo ◽

...

Keyword(s):

Prognostic Factors ◽

Expression Patterns ◽

Japanese Patients ◽

Monocarboxylate Transporter ◽

Two Factors ◽

Gpc3 Expression ◽

History Of ◽

Reciprocal Expression ◽

Immunohistochemical Analyses ◽

Monocarboxylate Transporter 4

Abstract Objective We previously reported the identification of monocarboxylate transporter 4 (MCT4) and glypican-3 (GPC3) as prognostic factors for hepatocellular carcinoma (HCC), which are now considered significant poor prognostic factors for the disease. This study aimed to clarify the detailed interaction of these two factors in HCC to improve our understanding of aggressive HCC phenotypes. A total of 225 Japanese patients with HCC from our previous study were subjected to immunohistochemical analyses. Results The number of MCT4-positive (MCT4+) HCC cases was 47 (21%), and most MCT4+ HCC showed high GPC3 expression (94%, 44/47 cases). In 44 MCT4+/GPC3+ HCC cases, intratumoral heterogeneity of GPC3 or MCT4 expression was further evaluated. We observed reciprocal (inverse), synergistic, mixed reciprocal and synergistic, or irrelevant interaction of MCT4 and GPC3 expression in 29 (66%), 5 (11%), 1 (2%), and 9 cases (21%), respectively. The cases exhibiting reciprocal expression of both markers tended to have cirrhosis without a history of neoadjuvant therapy. In summary, although MCT4+ HCC cases are mostly GPC3+, intratumoral expression patterns of MCT4 and GPC3 are frequently reciprocal each other, suggesting that dual targeting of MCT4 and GPC3 may achieve a better antitumor effect for MCT4+ HCC cases.

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A novel nutrient sensing mechanism underlies substrate-induced regulation of monocarboxylate transporter-1

AJP Gastrointestinal and Liver Physiology ◽

10.1152/ajpgi.00308.2012 ◽

2012 ◽

Vol 303 (10) ◽

pp. G1126-G1133 ◽

Cited By ~ 51

Author(s):

Alip Borthakur ◽

Shubha Priyamvada ◽

Anoop Kumar ◽

Arivarasu A. Natarajan ◽

Ravinder K. Gill ◽

...

Keyword(s):

Intestinal Inflammation ◽

Apical Membrane ◽

Nutrient Sensing ◽

Monocarboxylate Transporter ◽

Rat Colon ◽

Intracellular Camp ◽

Short Term ◽

Monocarboxylate Transporter 1 ◽

Sensing Mechanism ◽

And Function

Monocarboxylate transporter isoform-1 (MCT1) plays an important role in the absorption of short-chain fatty acids (SCFAs) in the colon. Butyrate, a major SCFA, serves as the primary energy source for the colonic mucosa, maintains epithelial integrity, and ameliorates intestinal inflammation. Previous studies have shown substrate (butyrate)-induced upregulation of MCT1 expression and function via transcriptional mechanisms. The present studies provide evidence that short-term MCT1 regulation by substrates could be mediated via a novel nutrient sensing mechanism. Short-term regulation of MCT1 by butyrate was examined in vitro in human intestinal C2BBe1 and rat intestinal IEC-6 cells and ex vivo in rat intestinal mucosa. Effects of pectin feeding on MCT1, in vivo, were determined in rat model. Butyrate treatment (30–120 min) of C2BBe1 cells increased MCT1 function {p-(chloromercuri) benzene sulfonate (PCMBS)-sensitive [14C]butyrate uptake} in a pertussis toxin-sensitive manner. The effects were associated with decreased intracellular cAMP levels, increased Vmax of butyrate uptake, and GPR109A-dependent increase in apical membrane MCT1 level. Nicotinic acid, an agonist for the SCFA receptor GPR109A, also increased MCT1 function and decreased intracellular cAMP. Pectin feeding increased apical membrane MCT1 levels and nicotinate-induced transepithelial butyrate flux in rat colon. Our data provide strong evidence for substrate-induced enhancement of MCT1 surface expression and function via a novel nutrient sensing mechanism involving GPR109A as a SCFA sensor.

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