Investigation of Brucella antibodies in bovine sera by rose bengal plate test (RBPT), serum agglutination test (SAT), microagglutination test (MAT) and 2-mercaptoethanol-microagglutination (2-ME-MAT)

An investigation was carried out to study the seroprevalence and risk factors for Brucella seropositivity in sheep in Bangladesh. For this, highly sheep populated ten different districts including all seven divisions of Bangladesh were selected where sheep of Panchagarh (151), Naogaon (101), Tangail (83), Noakhali (56), Sunamganj (52), Patuakhali (119) and Dhaka (75) were tested but sheep of Chapai Nawabganj, Khulna, Noakhali and Feni has under tested for Brucellosis. In the present study, seroprevalence of ovine brucellosis was tested by Rose Bengal Plate Test (RBPT), Serum Agglutination Test (SAT) and Enzyme-Linked Immunosorbent Assay (ELISA). About 5% sheep might have got Brucella infection. The highest Brucella antibody was observed in sheep of Tangail district (8.4%) followed by the sheep of Savar (8.0%), where as sheep of Subarna Char and Kala Para were free from Brucella antibody. In relation to age and sex, adults (6.5%) were more positive than young (3.6%) and female (5.3%) were more susceptible than male (3.8%).Asian J. Med. Biol. Res. March 2016, 2(1): 13-18

Download Full-text

Comparison of the results of some serological tests for bovine brucellosis

Journal of Hygiene ◽

10.1017/s0022172400024815 ◽

1978 ◽

Vol 80 (3) ◽

pp. 365-371 ◽

Cited By ~ 6

Author(s):

R. J. Chappel ◽

D. J. McNaught ◽

J. A. Bourke ◽

G. S. Allan

Keyword(s):

Rose Bengal ◽

Complement Fixation Test ◽

Complement Fixation ◽

Agglutination Test ◽

Bovine Brucellosis ◽

Serological Tests ◽

Plate Test ◽

Comparison Of The Results ◽

The Rose ◽

Serum Agglutination

SummaryA total of 1887 bovine sera positive to the Rose Bengal plate test were subjected to other serological tests for bovine brucellosis: the complement fixation test using warm fixation (CFTW), the serum agglutination test (SAT) and the radioimmunoassay (RIA).The SAT was generally much less sensitive than the CFTW. Many sera, however, gave positive reactions in the SAT but no reaction in the CFTW or the RIA. These SAT reactions were attributed to IgM antibody.Comparison between the results of the CFTW and the RIA led to the conclusion that 200 ng could be used as a minimum diagnostic reaction in the RIA.

Download Full-text

A quantitative comparison of the sensitivity of serological tests for bovine brucellosis to different antibody classes

Journal of Hygiene ◽

10.1017/s0022172400055182 ◽

1976 ◽

Vol 76 (2) ◽

pp. 287-298 ◽

Cited By ~ 30

Author(s):

G. S. Allan ◽

R. J. Chappel ◽

P. Williamson ◽

D. J. McNaught

Keyword(s):

Complement Fixation Test ◽

Complement Fixation ◽

Quantitative Comparison ◽

Agglutination Test ◽

Bovine Brucellosis ◽

Serological Tests ◽

Plate Test ◽

Specific Antibodies ◽

The Rose ◽

Serum Agglutination

SUMMARYBrucella-specific antibodies of different immunoglobulin classes were quantitatively evaluated with respect to their efficiency in serological tests for bovine brucellosis.IgM reacted more efficiently than IgG1and IgG2in both the Rose Bengal plate test and serum agglutination test. The complement fixation test was found to be slightly more sensitive to IgM than to IgG1and did not react to IgG2.IgM was, however, partly inactivated when heated at 60°C. in the presence of serum.

Download Full-text

Evaluation of Seven Tests for Diagnosis of Human Brucellosis in an Area Where the Disease Is Endemic

Clinical and Vaccine Immunology ◽

10.1128/cvi.00424-07 ◽

2008 ◽

Vol 15 (6) ◽

pp. 1031-1033 ◽

Cited By ~ 64

Author(s):

M. Concepción Gómez ◽

José A. Nieto ◽

Carmen Rosa ◽

Paloma Geijo ◽

M. Ángeles Escribano ◽

...

Keyword(s):

Rose Bengal ◽

Immunoglobulin G ◽

Agglutination Test ◽

Human Brucellosis ◽

Coombs Test ◽

Specific Antibodies ◽

Enzyme Immunoassays ◽

Serologic Tests ◽

Microagglutination Test ◽

Conventional Tests

ABSTRACT The results of seven serologic tests for diagnosis of human brucellosis were evaluated. The titrated Rose Bengal test, microagglutination test, microtiter-adapted Coombs test, and immunocapture-agglutination test (Brucellacapt) were positive for all sera from patients with acute brucellosis. The immunoglobulin G (IgG), IgM, and IgA commercial enzyme immunoassays (ELISAs) failed to show specific antibodies in 3 patients, 10 patients, and 1 patient, respectively. The sensitivity of ELISA is not higher than that of conventional tests.

Download Full-text

Socio-Demography Characteristics and Prevalence of Brucellosis Among Community Members in Kenya. A Case of Mandera East Sub-County, Mandera County, Kenya

International Journal of Healthcare and Medical Sciences ◽

10.32861/ijhms.511.59.65 ◽

2019 ◽

pp. 59-65

Author(s):

Abdirahman S. Abdalla

Keyword(s):

Rose Bengal ◽

Ethical Issues ◽

Group Discussion ◽

Specific Treatment ◽

Febrile Illness ◽

Agglutination Test ◽

Confirmatory Test ◽

Community Members ◽

Cross Sectional ◽

Plate Test

Brucellosis is an infectious debilitating, acute or sub-acute febrile illness usually marked by an intermittent or remittent fever accompanied by malaise, anorexia and prostration, and which, in the absence of specific treatment, may persist for weeks or months. The aim of this study was to determine the relationship between Socio-Demography Characteristics and prevalence of brucellosis among community members in Kenya. A case of Mandera East Sub-county, Mandera County, Kenya. The study was descriptive cross sectional study which collected both qualitative and quantitative data from where a sample of 420 respondents was systematically selected from heads of 2,617 households form Mandera East Sub-county. The study instruments included questionnaire, Focus Group Discussion guide and Interview Guide. Blood samples were screened for brucellosis using Rose Bengal Plate Test (RBPT) and the positive sera were subjected through Serum Slow Agglutination Test (SSAT) which acted as a confirmatory test. Data was analyzed using SPSS Version 20 and results of the study presented in frequencies and percentages in Tables and Figures. Ethical issues were observed and consent sought from the respondents. Rose Bengal Plate Test (RBPT) indicated a prevalence of 24.8% (95% CI: 20.0–29.6) and Serum Slow Agglutination Test (SSAT) indicated that the prevalence was at 14.3% (95% CI: 8.7–19.9) among the respondents. The study showed that the seroprevalence was higher among the male respondents (98%; n=103) as detected through RBPT and (98%; n=57) confirmed through SSAT. There was significant relationship between the gender and seroprevalence as tested through RBPT (P<0.001).

Download Full-text

Detection of Brucella Antibodies of Sheep in Al-Anbar Province by Using Some Serological Tests

The Iraqi Journal of Veterinary Medicine ◽

10.30539/iraqijvm.v37i1.316 ◽

2013 ◽

Vol 37 (1) ◽

pp. 7-12

Author(s):

Ekram A. Al-Samarrae

Keyword(s):

Rose Bengal ◽

Agglutination Test ◽

Infected Sheep ◽

Serological Tests ◽

Blood Samples ◽

Plate Test ◽

Sheep Blood ◽

Total Percentage ◽

Different Ages

An investigation was carried out to detect anti-Brucella antibodies of sheep in Al-Anbar province by using few serological tests; rose Bengal plate test (RBPT) and tube agglutination test (TAT). A total of 754 sheep blood samples (94 Ram and 660 Ewe) were collected randomly distributed in Al-Anbar province from unvaccinated sheep flocks with different ages. A total percentage of infected sheep depending on RBPT was 10.21% with significant differences at (P< 0.01), it was 9.69% in ewes, whereas in rams it elevated to reach 13.82%, in addition to the disease was higher seroprevalence at > 3 years (12.78%) in both gender with significant differences at (P<0.05) than lower ages. Out of 77 RBPT positive sera, 38(49.35%) were positive using TAT, there was 14 seropositive with significant differences at (P< 0.01). The study reported that brucellosis was distributed in Al-Anbar province with variable percent (3.26% to 21.27%), as well as emphasized on different epidemiological aspects.

Download Full-text