scholarly journals Socio-Demography Characteristics and Prevalence of Brucellosis Among Community Members in Kenya. A Case of Mandera East Sub-County, Mandera County, Kenya

2019 ◽  
pp. 59-65
Author(s):  
Abdirahman S. Abdalla
Keyword(s):  
Rose Bengal ◽  
Ethical Issues ◽  
Group Discussion ◽  
Specific Treatment ◽  
Febrile Illness ◽  
Agglutination Test ◽  
Confirmatory Test ◽  
Community Members ◽  
Cross Sectional ◽  
Plate Test

Brucellosis is an infectious debilitating, acute or sub-acute febrile illness usually marked by an intermittent or remittent fever accompanied by malaise, anorexia and prostration, and which, in the absence of specific treatment, may persist for weeks or months. The aim of this study was to determine the relationship between Socio-Demography Characteristics and prevalence of brucellosis among community members in Kenya. A case of Mandera East Sub-county, Mandera County, Kenya. The study was descriptive cross sectional study which collected both qualitative and quantitative data from where a sample of 420 respondents was systematically selected from heads of 2,617 households form Mandera East Sub-county. The study instruments included questionnaire, Focus Group Discussion guide and Interview Guide. Blood samples were screened for brucellosis using Rose Bengal Plate Test (RBPT) and the positive sera were subjected through Serum Slow Agglutination Test (SSAT) which acted as a confirmatory test. Data was analyzed using SPSS Version 20 and results of the study presented in frequencies and percentages in Tables and Figures. Ethical issues were observed and consent sought from the respondents. Rose Bengal Plate Test (RBPT) indicated a prevalence of 24.8% (95% CI: 20.0–29.6) and Serum Slow Agglutination Test (SSAT) indicated that the prevalence was at 14.3% (95% CI: 8.7–19.9) among the respondents. The study showed that the seroprevalence was higher among the male respondents (98%; n=103) as detected through RBPT and (98%; n=57) confirmed through SSAT. There was significant relationship between the gender and seroprevalence as tested through RBPT (P<0.001).

scholarly journals The Use of Rose Bengal Plate Test to Asses Cattle Exposure to Brucella Infection in Traditional and Smallholder Dairy Production Systems of Tanga Region of Tanzania

2010 ◽  
Vol 2010 ◽  
pp. 1-8 ◽  
Author(s):  
Emanuel Senyael Swai ◽  
Luuk Schoonman
Keyword(s):  
Risk Factors ◽  
Rose Bengal ◽  
Production Systems ◽  
Multivariate Logistic Regression Analysis ◽  
Control Measures ◽  
Serum Samples ◽  
Cross Sectional ◽  
Plate Test ◽  
Increased Risk ◽  
Smallholder Dairy

A cross-sectional epidemiological study was conducted to determine the seroprevalence and to identify risk factors for bovine brucellosis seropositivity in traditional and smallholder dairy cattle production systems in the Tanga region of North-eastern Tanzania. The study populations comprised 246 indigenous and 409 crossbred cattle, randomly selected from 105 smallholder dairy and 25 traditional managed herds, respectively. Individual animal and herd-level data were collected using a structured questionnaire. Serum samples were screened forBrucellaantibodies using the Rose Bengal Plate Test The overall seroprevalence ofBrucellaantibodies in the smallholder dairy and traditional managed cattle was 4.1% and 7.3% respectively. The corresponding overall herd prevalence was 10.5% and 20% respectively. Using multivariate logistic regression analysis, closeness to stock route, access to surface drinking water and location were identified as the major risk factors for individual herd seroprevalence. Older animals (6 years) were associated with increased risk of sero-positivity compared to animals of age category of 6 years. The results showed that brucellosis is prevalent and widely distributed locally, underscoring the need for further studies including surveillance and institution of preventive and control measures particularly among female young-stock and the general public who are at high risk of contracting brucellosis.

scholarly journals Detection of Brucella Antibodies of Sheep in Al-Anbar Province by Using Some Serological Tests

2013 ◽  
Vol 37 (1) ◽  
pp. 7-12
Author(s):  
Ekram A. Al-Samarrae
Keyword(s):  
Rose Bengal ◽  
Agglutination Test ◽  
Infected Sheep ◽  
Serological Tests ◽  
Blood Samples ◽  
Plate Test ◽  
Sheep Blood ◽  
Total Percentage ◽  
Different Ages

An investigation was carried out to detect anti-Brucella antibodies of sheep in Al-Anbar province by using few serological tests; rose Bengal plate test (RBPT) and tube agglutination test (TAT). A total of 754 sheep blood samples (94 Ram and 660 Ewe) were collected randomly distributed in Al-Anbar province from unvaccinated sheep flocks with different ages. A total percentage of infected sheep depending on RBPT was 10.21% with significant differences at (P< 0.01), it was 9.69% in ewes, whereas in rams it elevated to reach 13.82%, in addition to the disease was higher seroprevalence at > 3 years (12.78%) in both gender with significant differences at (P<0.05) than lower ages. Out of 77 RBPT positive sera, 38(49.35%) were positive using TAT, there was 14 seropositive with significant differences at (P< 0.01). The study reported that brucellosis was distributed in Al-Anbar province with variable percent (3.26% to 21.27%), as well as emphasized on different epidemiological aspects.

scholarly journals Seroprevalence of ovine brucellosis in Bangladesh

2016 ◽  
Vol 2 (1) ◽  
pp. 13-18 ◽  
Author(s):  
Md Osman Gani ◽  
Md Nuruzzaman Munsi ◽  
Md Ershaduzzaman ◽  
AKM Anisur Rahman ◽  
Sajeda Sultana ◽  
...  
Keyword(s):  
Risk Factors ◽  
Immunosorbent Assay ◽  
Rose Bengal ◽  
Agglutination Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Plate Test ◽  
Brucella Infection ◽  
Serum Agglutination ◽  
Age And Sex

An investigation was carried out to study the seroprevalence and risk factors for Brucella seropositivity in sheep in Bangladesh. For this, highly sheep populated ten different districts including all seven divisions of Bangladesh were selected where sheep of Panchagarh (151), Naogaon (101), Tangail (83), Noakhali (56), Sunamganj (52), Patuakhali (119) and Dhaka (75) were tested but sheep of Chapai Nawabganj, Khulna, Noakhali and Feni has under tested for Brucellosis. In the present study, seroprevalence of ovine brucellosis was tested by Rose Bengal Plate Test (RBPT), Serum Agglutination Test (SAT) and Enzyme-Linked Immunosorbent Assay (ELISA). About 5% sheep might have got Brucella infection. The highest Brucella antibody was observed in sheep of Tangail district (8.4%) followed by the sheep of Savar (8.0%), where as sheep of Subarna Char and Kala Para were free from Brucella antibody. In relation to age and sex, adults (6.5%) were more positive than young (3.6%) and female (5.3%) were more susceptible than male (3.8%).Asian J. Med. Biol. Res. March 2016, 2(1): 13-18

scholarly journals Comparison of the results of some serological tests for bovine brucellosis

1978 ◽  
Vol 80 (3) ◽  
pp. 365-371 ◽  
Author(s):  
R. J. Chappel ◽  
D. J. McNaught ◽  
J. A. Bourke ◽  
G. S. Allan
Keyword(s):  
Rose Bengal ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Agglutination Test ◽  
Bovine Brucellosis ◽  
Serological Tests ◽  
Plate Test ◽  
Comparison Of The Results ◽  
The Rose ◽  
Serum Agglutination

SummaryA total of 1887 bovine sera positive to the Rose Bengal plate test were subjected to other serological tests for bovine brucellosis: the complement fixation test using warm fixation (CFTW), the serum agglutination test (SAT) and the radioimmunoassay (RIA).The SAT was generally much less sensitive than the CFTW. Many sera, however, gave positive reactions in the SAT but no reaction in the CFTW or the RIA. These SAT reactions were attributed to IgM antibody.Comparison between the results of the CFTW and the RIA led to the conclusion that 200 ng could be used as a minimum diagnostic reaction in the RIA.

Seroprevalence of human brucellosis in rural endemic areas in eastern Turkey

2008 ◽  
Vol 38 (1) ◽  
pp. 42-43 ◽  
Author(s):  
Serhat Vancelik ◽  
Asuman Guraksin ◽  
Ahmet Ayyildiz
Keyword(s):  
Public Health ◽  
Rose Bengal ◽  
Health Problem ◽  
Public Health Problem ◽  
Agglutination Test ◽  
Human Brucellosis ◽  
Community Based ◽  
Cross Sectional ◽  
Rose Bengal Test ◽  
The Rose

In this cross-sectional, community-based study, sera were obtained from 573 subjects. Brucella seropositivity was detected in 5.4% according to the standard tube agglutination test, rising to 11.9% when the Rose-Bengal test was used. Brucellosis is a serious public health problem in eastern Tur key.

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