Elicitation of Anthocyanin Production in Roots of Kalanchoe blossfeldiana by Methyl Jasmonate

AbstractThe influence of methyl jasmonate on anthocyanin accumulation in roots ofKalanchoe blossfedianaplants was studied. Methyl jasmonate (JA-Me), at a concentration of 5.0 to 40.0 mg.l−1, substantially increased anthocyanin accumulation in roots of intact plants, when it was applied as a solution under natural light conditions. The production of anthocyanin depended on the concentration of methyl jasmonate and the age of the plant. The stimulatory effect was higher in older plants ofK. blossfeldianathan in younger ones. When leaves were removed methyl jasmonate slightly stimulated anthocyanin accumulation compared with intact plants. The obtained results indicate that leaves are necessary for the anthocyanin accumulation in the roots. In isolated roots methyl jasmonate did not affect the accumulation of anthocyanins in light conditions. Seven anthocyanins were documented in the roots of control plants and 8 anthocyanins in the roots of JA-Me treated ones. JA-Me increased the level of anthocyanins in roots of oldK. blossfeldianaplants 6.8, 6.0 and 3.6-folds, after 4, 8 and 14-days of treatment, respectively.

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The Effect Of Some Plant Growth Regulators And Their Combination With Methyl Jasmonate On Anthocyanin Formation In Roots Of Kalanchoe Blossfeldiana

Journal of Horticultural Research ◽

10.2478/johr-2014-0019 ◽

2014 ◽

Vol 22 (2) ◽

pp. 31-40 ◽

Cited By ~ 2

Author(s):

Justyna Góraj ◽

Elżbieta Węgrzynowicz-Lesiak ◽

Marian Saniewski

Keyword(s):

Abscisic Acid ◽

Plant Growth ◽

Methyl Jasmonate ◽

Plant Growth Regulators ◽

Growth Regulators ◽

Anthocyanin Accumulation ◽

Kalanchoe Blossfeldiana ◽

Isolated Roots ◽

Whole Plants ◽

Kalanchoë Blossfeldiana

AbstractIn this study, we investigated the effect of plant growth regulators (PGRs) - auxins, gibberellin, cytokinin, abscisic acid, brassinosteroid, ethylene and their interaction with methyl jasmonate (JA-Me) applied to roots of the whole plants Kalanchoe blossfeldiana on the accumulation of anthocyanins in roots. The highest stimulation of anthocyanins synthesis was stated with application of JA-Me alone. In response to treatments with the other tested PGRs, the content of anthocyanins in roots of a whole plant was different depending on the concentration of the PGR when being applied alone or together with JA-Me. Auxin, indole-3-acetic acid (IAA) at a concentration of 50 mg·L-1, indole-3-butyric acid (IBA) at 5 mg·L-1 and abscisic acid (ABA) at 10 mg·L-1 induced anthocyanin accumulation with approximately 60-115% compared to the control while 24-epibrassinolid (epiBL), gibberellic acid (GA3) and 6-benzylaminopurine (BAP) had no effect on the anthocyanin accumulation. The simultaneous administration of the PGRs with JA-Me usually resulted in the accumulation of anthocyanins in roots in a manner similar to that caused by JA-Me. PGRs applied to isolated roots did not stimulate anthocyanin accumulation, except for the combination of JA-Me with 50 mg·L-1 IAA. The results indicate that in K. blossfeldiana, the aboveground parts of the plant play an important role in the biosynthesis of anthocyanins in roots.

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The effect of sugars in relation to methyl jasmonate on anthocyanin formation in the roots of Kalanchoe blossfeldiana (Poelln.)

Acta Agrobotanica ◽

10.5586/aa.2015.015 ◽

2015 ◽

Vol 32 (2) ◽

pp. 173-178 ◽

Cited By ~ 1

Author(s):

Justyna Góraj-Koniarska ◽

Marian Saniewski

Keyword(s):

Methyl Jasmonate ◽

Anthocyanin Content ◽

Anthocyanin Accumulation ◽

Sugar Alcohols ◽

Anthocyanin Formation ◽

Intact Plants ◽

Kalanchoe Blossfeldiana ◽

Kalanchoë Blossfeldiana

This study investigated the effects of different sugars (sucrose, fructose, glucose) and sugar alcohols (mannitol, sorbitol) applied alone and in solution with methyl jasmonate (JA-Me) on the anthocyanin content in the roots of Kalanchoe blossfeldiana. None of the sugars used individually in the experiment affected anthocyanin accumulation in the roots of intact plants. The anthocyanin level was similar to that in the control. Sucrose at concentrations of 0.5% and 3.0%, and glucose at a concentration of 3.0% inhibited anthocyanin accumulation induced by JA-Me. Only fructose at a concentration of 3.0% stimulated anthocyanin accumulation induced by JA-Me. The sugar alcohols, mannitol at a concentration of 3.0% and sorbitol at 0.5% and 3.0%, inhibited anthocyanin accumulation in the roots of intact K. blossfeldiana plants induced by JA-Me. In excised roots, both sugars and JA-Me used individually did not affect the formation of anthocyanins. Also, the sugar alcohols (mannitol and sorbitol) applied simultaneously with JA-Me had no effect on the accumulation of anthocyanins. However, roots treated with sugars (sucrose, fructose, glucose) in solution with JA-Me promoted the induction of anthocyanins in the apical parts of the roots. The results suggest that anthocyanin elicitation in the roots of K. blossfeldiana by methyl jasmonate may be dependent on the interaction of JA-Me with sugars transported from the stems (leaves) to the roots.

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Fatty acid and sterol contents during methyl jasmonate-induced leaf abscission in Kalanchoe blossfeldiana

Acta Agrobotanica ◽

10.5586/aa.1994.015 ◽

2013 ◽

Vol 47 (2) ◽

pp. 83-88

Author(s):

Marian Saniewski ◽

Janusz Czapski ◽

Marcin Horbowicz

Keyword(s):

Fatty Acids ◽

Fatty Acid ◽

Methyl Jasmonate ◽

Leaf Abscission ◽

Kalanchoe Blossfeldiana ◽

Kalanchoë Blossfeldiana

It was found previously that methyl jasmonate (JA-Me) induced leaf abscission in Kalanchoe blossfeldiana. In present studies it was showed that JA-Me did not affect or only slightly affected the content of free and bound fatty acids in petioles and blades. ß-Sitosterol, campesterol and ß-amyrin were identified in petioles and blades of K. blossfeldiana; JA-Me decreased the content of campesterol in petioles and increased the content of ß-sitosterol in blades. In blades of plants treated with JA-Me disappearance of olean-12-one was indicated but appearance of 2H-cyclopropa[a]-naphthalen-2-one,l, la, 4, 5, 6, 7, 7a, 7b-octahydro-l, 1, 7, 7a-tetramethyl (aristolone) was documented. The significance of these findings in leaf abscission induced by methyl jasmonate in K. blossfeldiana is discussed.

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Methyl Jasmonate- and Light-Induced Glucosinolate and Anthocyanin Biosynthesis in Radish Seedlings

Natural Product Communications ◽

10.1177/1934578x1501000719 ◽

2015 ◽

Vol 10 (7) ◽

pp. 1934578X1501000 ◽

Cited By ~ 1

Author(s):

Naif Abdullah Al-Dhabi ◽

Mariadhas Valan Arasu ◽

Sun Ju Kim ◽

Md. RomijUddin ◽

Woo Tae Park ◽

...

Keyword(s):

Methyl Jasmonate ◽

Anthocyanin Biosynthesis ◽

Anthocyanin Accumulation ◽

Light Conditions ◽

Asian Countries ◽

Low Concentrations ◽

Meja Treatment ◽

Radish Sprouts ◽

Dark Conditions ◽

Radish Seedlings

Radish sprouts and young seedlings are considered important dietary vegetables in Asian countries. In this study, we investigated the levels of glucosinolate and anthocyanin accumulation in radish seedlings in response to light and methyl jasmonate (MeJA) treatments. MeJA facilitated the accumulation of glucosinolate and anthocyanins under light conditions. The glucosinolate and anthocyanin contents in the radish seedlings that were exposed to light after MeJA treatment were higher than those of the seedlings that were grown in the dark without MeJA. At a concentration of 100 μM, MeJA led to the greatest accumulation of the most glucosinolates under both light and dark conditions. Under light conditions, the levels of glucoraphenin, glucoerucin, and glucotropaeolin accumulation were 1.53-, 1.60-, and 1.30-fold higher, respectively, than those of the control. Remarkable accumulations of glucobrassicin were observed under light conditions (4.4-, 6.7-, and 7.8-fold higher than that of the control following the application of 100, 300, and 500 μM MeJA, respectively). The level of cyanidin in the 300μM MeJA-treated seedlings was double of that in the control without MeJA treatment. The highest level of pelargonidin was observed after treatment with 500 μMMeJA under light conditions; this level was 1.73times higher than that in the control. A similar trend of anthocyaninaccumulation was observed in the radish seedlings following MeJA treatment under dark conditions, but the levels of anthocyanins were considerably lower in the seedlings that were grown in the dark. Our findings suggest that light and low concentrations of MeJA enhance the accumulations of glucosinolates and anthocyanins during the development of radish seedlings.

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Methyl jasmonate stimulates the formation and the accumulation of anthocyanin in Kalanchoe blossfeldiana

Acta Physiologiae Plantarum ◽

10.1007/s11738-003-0047-7 ◽

2003 ◽

Vol 25 (2) ◽

pp. 143-149 ◽

Cited By ~ 19

Author(s):

Marian Saniewski ◽

Marcin Horbowicz ◽

Jerzy Puchalski ◽

Junichi Ueda

Keyword(s):

Methyl Jasmonate ◽

Kalanchoe Blossfeldiana ◽

Kalanchoë Blossfeldiana

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Activity of cell wall degrading glycanases in methyl jasmonate-induced leaf abscission in Kalanchoe blossfeldiana

Acta Agrobotanica ◽

10.5586/aa.1995.008 ◽

2013 ◽

Vol 48 (1) ◽

pp. 69-74 ◽

Cited By ~ 2

Author(s):

Marian Saniewski ◽

Ewa Gajewska ◽

Henryk Urbanek

Keyword(s):

Cell Wall ◽

Methyl Jasmonate ◽

Apical Part ◽

Abscission Zone ◽

Leaf Abscission ◽

Cell Wall Polysaccharides ◽

Kalanchoe Blossfeldiana ◽

Kalanchoë Blossfeldiana

It was found previously that methyl jasmonate (JA-Me) induced leaf abscission in Kalanchoe blossfeldiana. In present studies it was shown that JA-Me markedly increased the total activities of cellulase, polygalacturonase, pectinase and xylanase in petioles, but did not affect activities of these enzymes in the blades and apical part of shoots of K. blossfeldiana. These results suggest that methyl jasmonate promotes the degradation of cell wall polysaccharides in the abscission zone and in this way induces leaf abscission in Kalanchoe blossfeldiana.

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METHYL JASMONATE-INDUCED LEAF ABSCISSION IN KALANCHOE BLOSSFELDIANA

Acta Horticulturae ◽

10.17660/actahortic.1995.394.34 ◽

1995 ◽

pp. 315-324 ◽

Cited By ~ 7

Author(s):

M. Saniewski ◽

E. Wegrzynowicz-Lesiak

Keyword(s):

Methyl Jasmonate ◽

Leaf Abscission ◽

Kalanchoe Blossfeldiana ◽

Kalanchoë Blossfeldiana

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First Report of Powdery Mildew of Kalanchoe blossfeldiana caused by Sphaerotheca fuliginea (Podosphaera fuliginea) in the Pacific Northwest

Plant Health Progress ◽

10.1094/php-2003-0417-02-hn ◽

2003 ◽

Vol 4 (1) ◽

pp. 39

Author(s):

Dean A. Glawe ◽

Rita Hummel ◽

Grace Jack

Keyword(s):

Powdery Mildew ◽

Pacific Northwest ◽

Sphaerotheca Fuliginea ◽

First Report ◽

Major Disease ◽

The Pacific ◽

Kalanchoe Blossfeldiana ◽

Extension Center ◽

Kalanchoë Blossfeldiana

Kalanchoe blossfeldiana Poelln. is a common ornamental houseplant. Although powdery mildew is a major disease of this species, there are no published reports of it in the Pacific Northwest. In August, 2002, powdery mildew was observed on six indoor K. blossfeldiana plants in an office and adjacent laboratory at the Puyallup Research and Extension Center. Accepted for publication 25 March 2003. Published 17 April 2003.

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