scholarly journals Synchronization Protocols in Spanish Merino Sheep: Reduction in Time to Estrus by the Addition of eCG to a Progesterone-Based Estrus Synchronization Protocol

2015 ◽  
Vol 15 (2) ◽  
pp. 409-418 ◽  
Author(s):  
Juan Carlos Gardón ◽  
Begoña Escribano ◽  
Susana Astiz ◽  
Salvador Ruiz
Keyword(s):  
Breeding Season ◽  
Chorionic Gonadotropin ◽  
Economic Efficiency ◽  
Medroxyprogesterone Acetate ◽  
Pregnancy Rates ◽  
Estrus Synchronization ◽  
Merino Sheep ◽  
Ewe Lambs ◽  
Equine Chorionic Gonadotropin ◽  
Synchronization Protocol

Abstract An appropriate management of reproduction allows ewes to breed at a more intensive rhythm, more compatible with economic efficiency of farms. Hence, the objectives of the present work were to evaluate a traditional estrus synchronization protocol (based on medroxyprogesterone acetate-impregnated intravaginal sponges combined or not with equine chorionic gonadotropin) on a Merino sheep herd during the breeding season, in order to determine the best working protocol for this breed. A total of 277 sheep, blocked by age (200 ewes and 77 ewe lambs) were randomly allocated to four groups, two of ewes (E and Ee) and two of ewe lambs (L and Le). All females received 60 mg MAP (medroxyprogesterone acetate) sponges on the first day. At the day of removal the animals of groups Ee and Le received 450 IU of eCG (equine chorionic gonadotropin). Mean estrus synchronization rate was 92.06% and similar among groups. Time to estrus was shorter for Ee (46.93±12.44 h) and Le (44.34±10.76 h), when compared to treatments without eCG (60.60±20.46 h and 58.68±17.72 h for E and L, respectively; P<0.05 for both). Pregnancy rates were 71.35 and 72.86% for ewes and ewe lambs, respectively, and similar among groups. Lambing rates were lower in ewes (1.36) than in ewe lambs (1.85) with no significant effect of eCG. The study demonstrates the efficacy of the synchronization method with MAP and the reduction in the time to estrus induced by eCG in Spanish Merino ewes and ewe lambs during the breeding season.

scholarly journals Effect of barium selenate injections on fertility of Pirlak ewes subjected to estrus synchronization during non-breeding season

2017 ◽  
Vol 73 (8) ◽  
pp. 479-482 ◽  
Author(s):  
Mushap Kuru ◽  
Osman Sogukpinar ◽  
Mustafa Makav ◽  
Nebi Cetin
Keyword(s):  
Pregnancy Rate ◽  
Breeding Season ◽  
Litter Size ◽  
Chorionic Gonadotropin ◽  
Conception Rate ◽  
Transrectal Ultrasonography ◽  
Estrus Synchronization ◽  
Equine Chorionic Gonadotropin ◽  
Synchronization Protocol ◽  
Lambing Rate

This study was aimed at determining the effect of barium selenate injections on the fertility of Pirlak ewes which had been subjected to progesterone-assisted estrus synchronization during the non-breeding season. A total of 150 Pirlak ewes between the ages of 2 and 5 years were used in the study. Sponges containing progesterone were inserted into the vaginas of the ewes for 11 (groups I and III) or 14 days (groups II and IV) for the purpose of estrus synchronization, and 500 IU equine chorionic gonadotropin (eCG) was administered on the day of sponge removal. In addition, barium selenate was injected s.c. immediately before estrus synchronization protocol in groups I and III. After the sponges had been removed, estrus was observed for four days. The ewes were mated with fertile rams. The pregnancy was determined by transrectal ultrasonography 30 days after mating. It was observed that the estrus rate, pregnancy rate, conception rate, lambing rate, twinning rate, and litter size were not statistically different (P > 0.05) between the groups, but estrus onset was different (P < 0.001). In conclusion, barium selenate injections did not affect the fertility parameters before progesterone-assisted synchronization was applied to the Pirlak ewes during the non-breeding season

scholarly journals Effect of GnRH and D-Chloprostenol application on pregnancy and prolificacy rates on Pelibuey ewes

2013 ◽  
pp. 3612-3617 ◽  
Author(s):  
Jaime Martínez T ◽  
Oziel Montañez V ◽  
Alejandro Ley De C ◽  
Fernando Izaguirre F ◽  
María Velazco Z ◽  
...  
Keyword(s):  
Chorionic Gonadotropin ◽  
Medroxyprogesterone Acetate ◽  
Fixed Time ◽  
Average Concentration ◽  
Gonadotropin Releasing Hormone ◽  
Pregnancy Rates ◽  
Ovarian Activity ◽  
Releasing Hormone ◽  
Natural Mating ◽  
Equine Chorionic Gonadotropin

ABSTRACTObjective. Was to evaluate the effect of GnRH and D-Chloprostenol application on pregnancy and prolificacy rates on Pelibuey ewes. Materials and methods. Forty five ewes were randomly allocated to one of three treatments: T1(n=15), day 0: sponges with 65 mg medroxyprogesterone acetate (MPA) + 200 IU equine chorionic gonadotropin (eCG) and sponge removal (day 12) + breeding by natural mating (days 12-15); T2 (n=15), day 0: 50 μg gonadotropin releasing hormone (GnRH) + 7.5 mg D-Chloprostenol (day 5) + 50 μg GnRH (day 7) + insemination at fixed time (AIFT) 12 to 14 h after last injection of GnRH; T3 (n=15), 100 μg GnRH (day 0) + 7.5 mg D-Chloprostenol (day 5) + 100 μg GnRH (day 7) + AIFT 12 to 14 h after last injection of GnRH. Results. The average concentration of progesterone (P4) in blood was 1.22 ± 0.74 ng/mL, which was used to verify ovarian activity at the beginning of the treatments. 100% of the T1 ewes presented estrus, beginning at 38.4±9.56 h after sponge removal. There were differences (p<0.05) for pregnancy rates, of 60, 33.33 and 46.66% respectively, among the treatments. Prolificacy was no different (p>0.05) among the treatments where the values were 1.2, 1.4 and 1.4 lambs/ewe for T1, T2 and T3, Conclusions. The results of this study show that the use of GnRH and D-Chloprostenol did improve pregnancy rates but did not improve prolificacy in tropical ewes

scholarly journals EFFECT OF KISPEPTIN-10 AS AN ALTERNATIVE TO ECG IN ESTRUS SYNCHRONIZATION PROTOCOL ON IMPROVING THE REPRODUCTIVE PERFORMANCE OF KARADI EWES

2021 ◽  
Vol 52 (3) ◽  
pp. 535-546
Author(s):  
T. A. Abdulkareem ◽  
S. J. Muhammad ◽  
A. N. Yousif
Keyword(s):  
Body Weight ◽  
Chorionic Gonadotropin ◽  
Reproductive Performance ◽  
The Other ◽  
Control Group ◽  
Estrus Synchronization ◽  
Serum Progesterone ◽  
Live Body Weight ◽  
Equine Chorionic Gonadotropin ◽  
Synchronization Protocol

This study was conducted to examine the effect of Kisspeptin-10 as an alternative to equine chorionic gonadotropin (eCG) in estrus- synchronization protocol for improving the reproductive performance of Karadi ewes. Forty adult ewes of 3-7 years old and 65–70 kg live body weight were equally divided into four equal groups. Animals were inserted with a progestagen (60 mg MAP)-impregnated sponges for 13 days as estrus synchronization protocol. Following withdrawal of sponges, first group was served as control (C) and intramuscularly injected with normal saline only. The second group was intramuscularly injected with 250 IU of eCG (eCG), while those of third and fourth groups were intravenously injected with 4 and 8 µg /kg body weight of Kisspeptin-10, referred as Kisspeptin1 and Kisspeptin2 groups respectively. Serum progesterone and LH concentrations did not significantly differ among groups before estrus synchronization protocol and eCG or Kisspeptin treatments. The two Kisspeptin-injected groups attained better (P≤0.01) overall mean estrus (100%), fertility (90%), conception (90 and 100%, respectively), lambing (90%), and twinning (10%) rates with lesser barrenness (10%) as compared with the other groups. Moreover, eCG and Kisspeptin groups exhibited greater (P≤ 0.01) percentage of increasing fecundity (233%) and extra lambs born (0.7) than the control group. In conclusion, treatment of Karadi ewes either with Kisspeptin-10 or eCG hormones enhanced the overall reproductive performance, but did not affect serum progesterone and LH concentrations.

scholarly journals Use of hormonal subdoses applied in Bai Hui acupuncture in estrus synchronization protocols for goats

2019 ◽  
Vol 40 (4) ◽  
pp. 1501 ◽  
Author(s):  
Diego Oliveira de Souza ◽  
Monna Lopes de Araújo ◽  
Carmo Emanuel Almeida Biscarde ◽  
Claudinéia da Silva Mendes ◽  
Mariana Alves de Andrade Silva ◽  
...  
Keyword(s):  
Estrus Synchronization ◽  
Reproductive Parameters ◽  
Follicular Growth ◽  
Plasma Progesterone ◽  
Significant Difference ◽  
Estrus Detection ◽  
Detection Coverage ◽  
Equine Chorionic Gonadotropin ◽  
The Cost ◽  
Synchronization Protocol

The objective of this study was to evaluate the efficacy of delivering reduced doses of hormones via the Bai Hui acupoint in estrus synchronization in goats. A total of 40 goats received intravaginal sponges with medroxyprogesterone acetate for 7 days. The goats were then randomly distributed into 5 treatment: T1 - application of 132.5 ?g of cloprostenol and 300 IU of equine chorionic gonadotropin (eCG), both by intramuscular injection (IM); T2 - application of 39.75 ?g cloprostenol at the Bai Hui acupoint, and 300 IU of eCG by IM; T3 - application of 132.5 ?g of cloprostenol by IM, and 90 IU of eCG at the Bai Hui acupoint; T4 - application of 39.75?g of cloprostenol and 90 UI of eCG, both in Bai Hui and T5 acupuncture: application of 39.75?g of cloprostenol and 90 UI of eCG, both applied in false acupoint. The goats were subjected to an estrus synchronization protocol and monitored for estrus detection, coverage and evaluation of reproductive parameters to detect entry into estrus. The data were subjected to normality tests, followed by appropriate statistical analyses of each variable. There was no significant difference (P > 0.05) in the percentage of animals in estrus (95.00 ± 11.18%), interval between sponge removal and beginning of estrus (49.72 ± 8.93 h), interval between sponge removal and end of estrus (76.84 ± 11.98 h), duration of estrus (27.08 ± 8.68 h), size of the largest follicle (6.82 ± 0.44 mm), interval between sponge removal and ovulation (78.28 ± 10.82 h), time from ovarian onset to estrus (28.52 ± 5.44 h), follicular growth rate (0.86 ± 0.29 mm/day), number of ovulations (1.32 ± 0.23), plasma progesterone concentration at 7 days after ovulation (10.28 ± 1.65 ng.mL-1), and gestation rate at 30 days after the beginning of estrus (75 ± 12.5%). However, the cost of the synchronization protocol per animal was 43.42% lower in treatments 4 and 5 (30% of the doses) than in treatment 1 (100% of the dose). Ovulation and estrus were efficiently synchronized with the use of 39.75 ?g of sodium cloprostenol and 90 UI of eCG, applied at the Bai Hui acupoint or at a false acupoint.

Efficiency of mating, artificial insemination or resynchronisation at different times after first timed artificial insemination in postpartum Nellore cows to produce crossbred calves

2019 ◽  
Vol 59 (2) ◽  
pp. 225 ◽  
Author(s):  
Walvonvitis Baes Rodrigues ◽  
Jean do Prado Jara ◽  
Juliana Correa Borges ◽  
Luiz Orcirio Fialho de Oliveira ◽  
Urbano Pinto Gomes de Abreu ◽  
...  
Keyword(s):  
Pregnancy Rate ◽  
Breeding Season ◽  
Chorionic Gonadotropin ◽  
Artificial Insemination ◽  
Fixed Time ◽  
Beef Cows ◽  
Device Removal ◽  
Oestradiol Benzoate ◽  
Timed Artificial Insemination ◽  
Equine Chorionic Gonadotropin

The objective of this trial was to evaluate different post-timed artificial insemination (TAI) reproductive managements in postpartum beef cows to produce crossbred calves from artificial insemination (AI). Nellore cows (n = 607), with 45 days postpartum, were inseminated at a fixed time, using a protocol that included an intravaginal progesterone-releasing device along with oestradiol benzoate, prostaglandin, equine chorionic gonadotropin, and oestradiol cypionate, followed TAI 48 h post-device removal. Four post-TAI treatments were evaluated: in CONTROL (T1, n = 161), cows were exposed to Nellore clean-up bulls until the end of the breeding season (75 days). In OBSERVATION (T2, n = 132), heat detection was performed for 15–25 days post-TAI, followed by AI. In RESYNC22 (T3, n = 157) and RESYNC30 (T4, n = 157), resynchronisation started after 22 or 30 days, following second TAI at Day 32 or 40 days after first TAI. In T2, T3 and T4, after the second AI, cows were exposed to Nellore clean-up bulls until the end of the breeding season (75 days). The pregnancy rate (PR) for the first TAI did not differ (54.6%, 53.0%, 59.2%, and 51.6% for CONTROL, OBSERVATION, RESYNC 22, and RESYNC 30, respectively; P = 0.66), and no difference was observed for the second TAI (RESYNC 22 = 45.31% and RESYNC30 = 46.05%; P = 0.137), in the PR at the end of the breeding season (86.33%, 86.36%, 78.98%, and 81.52%, P = 0.43), or embryonic losses (4.54%, 2.85%, 6.45% and 7.40%, respectively; P = 0.61), but the percentage of crossbred pregnancy was higher in groups with resynchronisation (RESYNC22 and RESYNC30) than CONTROL and OBSERVATION (98.38%, 90.62%, 63.30%, 78.95%, P &lt; 0.001). In conclusion, resynchronisation programs of 22 or 30 days are more efficient to produce AI products, and the final pregnancy rate is similar among the treatments, differing only in the amount of calves produced by AI.

Equine chorionic gonadotropin (eCG) enhances reproductive responses in CIDR-EB treated lactating anovular Nili-Ravi buffalo during the breeding season

2018 ◽  
Vol 196 ◽  
pp. 28-34 ◽  
Author(s):  
Abdul Saboor Khan ◽  
Muhammad Shafiq Haider ◽  
Mubbashar Hassan ◽  
Ali Husnain ◽  
Muhammad Rizwan Yousuf ◽  
...  
Keyword(s):  
Breeding Season ◽  
Chorionic Gonadotropin ◽  
Equine Chorionic Gonadotropin

scholarly journals 215SUCCESSFUL OUT-OF-BREEDING SEASON ESTRUS SYNCHRONIZATION FOLLOWED BY FIXED TIME INSEMINATION IN WATERBUFFALO (BUBALUS BUBALIS)

2004 ◽  
Vol 16 (2) ◽  
pp. 229 ◽  
Author(s):  
R.M. Züge ◽  
U. Rodacki ◽  
A.T. Grandi ◽  
J.M.J. Aerts ◽  
P.E.J. Bols
Keyword(s):  
Breeding Season ◽  
Buenos Aires ◽  
Fixed Time ◽  
Estradiol Benzoate ◽  
Sao Paulo ◽  
Reproductive Efficiency ◽  
São Paulo ◽  
Estrus Synchronization ◽  
The Impact ◽  
Synchronization Protocol

The most important barrier to the increase of buffalo productivity is an overall poor reproductive efficiency, characterized by late sexual maturity, seasonal anestrus and long periods of postpartum ovarian inactivity resulting in extended calving intervals and poor expression of estrus behavior (Singh J et al., 2000, Anim. Reprod. Sci. 60–61, 593–604). Buffaloes are seasonal breeders with the highest reproductive activities during winter (short day lengths) and a high frequency of anestrus during the summer months (Singh G et al. 1985, Ind. J. Anim. Res. 19, 57–60). Recent research demonstrated that a combination of progesterone, estradiol benzoate and equine chorionic gonadotropin (eCG) was effective for estrus induction and synchronization in buffalo heifers under Mediterranean conditions (Barile et al. 2001, Livestock Prod. Sci. 68, 283–287). The aim of the present study was to investigate the impact of an estrus synchronization protocol on reproductive efficiency of water buffalo during out of the normal breeding season. A total of six heifers (21 to 23 months of age) and three cows (5, 6 and 18 years of age) were enrolled in an estrus synchronization protocol lasting for 12 days. All animals were kept under tropical conditions in the coastal part of Paraná (Antonina), about 450km south of São Paulo. The experiment was performed in December, 2002, during the Brazilian summer season, when reproductive efficiency of buffaloes is greatly reduced. On the first day of the protocol (Day 0), animals were implanted with an intravaginal device containing 1g of progesterone (DIB, Syntex SA, Buenos Aires, Argentina) and injected with 10mg estradiol benzoate (Estrogin, Famavet, São Paulo, Brazil). On Day 9, the DIB implant was removed and the animals received 150μg (i.m.) of cloprostenol (Prolise, Syntex SA, Buenos Aires, Argentina) and 2500 IU of eCG (Novormon, Syntex SA, Buenos Aires, Argentina). On Day 11, all animals received 1500 IU of hCG (Vetecor, Lab. Calier, Spain). Artificial insemination (AI) was performed on Day 12 using frozen-thawed semen from a bull of proven fertility. Only one AI was performed per heifer/cow. Pregnancies were determined by ultrasound examination at 53 days following AI and confirmed by rectal palpation at 90 days post AI. The use of this estrus synchronization protocol, followed by fixed-time insemination, resulted in four pregnant heifers (66%) and three pregnant cows (100%). Our results demonstrate that buffalo reproduction can be successful during out-of-breeding season when adequate hormonal treatment is used. Additional experiments should be done to validate the protocol.

Effects of presynchronization and eCG on pregnancy rates to GnRH-based, fixed-time artificial insemination in beef heifers

2010 ◽  
Vol 90 (1) ◽  
pp. 23-34 ◽  
Author(s):  
J A Small ◽  
M G Colazo ◽  
J P Kastelic ◽  
N E Erickson ◽  
R J Mapletoft
Keyword(s):  
Chorionic Gonadotropin ◽  
Artificial Insemination ◽  
Follicular Development ◽  
Fixed Time ◽  
Pregnancy Rates ◽  
Preovulatory Follicle ◽  
Beef Heifers ◽  
Plasma Progesterone ◽  
Follicle Diameter ◽  
Equine Chorionic Gonadotropin

Three experiments were conducted to determine the effects of presynchronization and treatment with equine chorionic gonadotropin (eCG) on corpus luteum (CL) and ovarian follicular development, plasma progesterone concentrations, and pregnancy rates in beef heifers subjected to a gonadotropin releasing hormone (GnRH)-based, fixed-timed AI (TAI) protocol. All heifers were given GnRH on day 0, prostaglandin F2α (PGF) on day 7, and a second GnRH on day 9 concurrent with TAI (54 h after PGF). In exp. 1 (N = 148), presynchronization with PGF (days -22 and -11) decreased the percentage of heifers with non-luteal plasma progesterone concentrations on day 0 (5.4 vs 29.7%) and day 7 (0 vs 11.6%; P < 0.05), but not on day 9 (74.3 vs. 66.2%; P > 0.20), and reduced the number of heifers in estrus and bred before TAI (P < 0.05). Although presynchronization reduced preovulatory follicle diameter (12.9 ± 0.3 vs. 14.9 ± 0.3 mm; mean ± SEM; P < 0.01), it did not affect TAI pregnancy rates (36.5 vs. 29.7%; P > 0.20). In exp. 2, heifers (N = 128) were presynchronized with melengestrol acetate (MGA) (days -27 to -12), and received a controlled internal drug release (CIDR) on day 0; on day 7, half were given 300 IU of eCG at CIDR removal. Treatment with eCG tended to increase preovulatory follicle diameter in heifers that did not ovulate to GnRH on day 0 (P = 0.06), but did not affect the percentage of heifers with non-luteal plasma progesterone concentrations on day 9 (57.8 vs. 57.8%) or TAI pregnancy rates (48.4 vs. 53.1%; P > 0.20). Experiment 3 was a 2 × 2 factorial arrangement of presynchronization (PGF concurrent with a CIDR on day -7) and eCG treatments (on day 7) applied to heifers in three herds (A, N = 150, B, N = 260 and C, N = 40). All heifers had a once-used CIDR from days 0 to 7. Presynchronization increased the percentage of heifers (Herd A) with low-luteal plasma progesterone concentrations on day 0 (70.7 vs. 22.7%) and day 7 (90.7 vs. 53.3%; P < 0.01), but did not affect the percentage of heifers with non-luteal concentrations of progesterone on day 9 (97.3 vs. 93.3%; P > 0.20). Combined for all herds, presynchronization reduced the prevalence of a CL on day 0 (23.5 vs. 73.7%; P < 0.01), and increased the prevalence of follicles ≥ 10 mm on day 7 (96.8 vs. 86.7%; P < 0.01); however, TAI pregnancy rates (195/439 = 44.4%) were not improved by either presynchronization or eCG treatment (P > 0.20).Key words: Presynchronization, equine chorionic gonadotropin, GnRH, fixed-time artificial insemination, progesterone

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