scholarly journals EFFECT OF KISPEPTIN-10 AS AN ALTERNATIVE TO ECG IN ESTRUS SYNCHRONIZATION PROTOCOL ON IMPROVING THE REPRODUCTIVE PERFORMANCE OF KARADI EWES

2021 ◽  
Vol 52 (3) ◽  
pp. 535-546
Author(s):  
T. A. Abdulkareem ◽  
S. J. Muhammad ◽  
A. N. Yousif

This study was conducted to examine the effect of Kisspeptin-10 as an alternative to equine chorionic gonadotropin (eCG) in estrus- synchronization protocol for improving the reproductive performance of Karadi ewes. Forty adult ewes of 3-7 years old and 65–70 kg live body weight were equally divided into four equal groups. Animals were inserted with a progestagen (60 mg MAP)-impregnated sponges for 13 days as estrus synchronization protocol. Following withdrawal of sponges, first group was served as control (C) and intramuscularly injected with normal saline only. The second group was intramuscularly injected with 250 IU of eCG (eCG), while those of third and fourth groups were intravenously injected with 4 and 8 µg /kg body weight of Kisspeptin-10, referred as Kisspeptin1 and Kisspeptin2 groups respectively. Serum progesterone and LH concentrations did not significantly differ among groups before estrus synchronization protocol and eCG or Kisspeptin treatments. The two Kisspeptin-injected groups attained better (P≤0.01) overall mean estrus (100%), fertility (90%), conception (90 and 100%, respectively), lambing (90%), and twinning (10%) rates with lesser barrenness (10%) as compared with the other groups. Moreover, eCG and Kisspeptin groups exhibited greater (P≤ 0.01) percentage of increasing fecundity (233%) and extra lambs born (0.7) than the control group. In conclusion, treatment of Karadi ewes either with Kisspeptin-10 or eCG hormones enhanced the overall reproductive performance, but did not affect serum progesterone and LH concentrations.

2016 ◽  
Vol 47 (4) ◽  
Author(s):  
Al-Jubouri & Hobi

 The objective of this study was to carry out an on-farm experiment in sheep owner farms in Kanaan district for the period from 7th Feb. 2015 to 27th Mar. 2015 and to identify the unconventional methods in controlling the reproduction in sheep and increase their productivity using 45 local Awassi ewes, randomly selected from three sheep owners (15 ewes / owner). Treatment group (10 ewes/ owner) were treated with a vaginal sponges containing 20 mg fluorogestone acetate (FGA) inserted for 10 days, followed by I.M. injection of 500 IU equine chorionic gonadotropin (eCG), while 5 ewes / owner were regarded as control group without any treatment. Estrus was observed for all ewes for 5 days post withdrawal of sponges. Ewes that showed estrus were mated by rams in their flocks, thereafter, pregnancy detection was conducted at days 35-37 post- mating using transabdominal ultrasonography. A questionnaire involved 20 sheep owners and a demonstrative field day was conducted for owners contributed in the experiment, and others from the region of study to explain the importance and results of the experiment. The results showed the appearance of estrus in all ewes 100% within three days after the withdrawal of sponges. Pregnancy rate of  85.71% at days 35-37 of mating. The questionnaire showed there is an urgent need of knowledge for owners to find out the importance of controlling the reproductive cycle in sheep using vaginal sponges. Also, there were a correlation between the need of knowledge for sheep owners with (educational level for most of them, herd sizes, income from breeding, training courses and other activities in the fields of breeding and reproduction of sheep). In conclusion, the technology of estrus synchronization has an important role to improve reproductive performance of sheep and pregnancy could be detected through transabdominal ultrasonography at 35-37 days with high accuracy as well as there is great desire for the most owner to conduct estrus synchronization technique and to take care their birth of herds simultaneously.


2021 ◽  
Author(s):  
Ahmed Ismail Damarany

Abstract The current investigation was designed to determine the effect of treatment with anti-parasites (Ivermectin) on mastitis and postpartum reproductive efficiency measurements in Egyptian Baladi cows. Twenty-four of the cows included in this study were split into two groups. The first group (n = 12 cows) was injected two months prepartum with two doses (15 days inter-treatment) of IVOMEC® Plus solution subcutaneously behind the shoulder, 1ml/50kg live body weight as prescribed by the manufacturer, whilst the other group (n = 12 cows) was used as a control group. The present results revealed that the incident rate of mastitis in treated cows’ group was significantly (P < 0.05) lower (16.7%) than control cows’ group (41.7%). The conception rate in treated cows’ group was significantly (P < 0.05) higher (66.7%) compared with (33.3%) in control group. The time from calving to conception in treated cows was significantly (P < 0.05) lower 86.5 ± 11.4 days compared to 123.3 ± 13.2 days in the control group. The occurrence rate of anestrous cases was significantly (P < 0.05) lower (8.3%) in treated cows than (control group) (25%). In conclusion, the current study indicates there is an association between treated cows with anti-parasites (Ivermectin) two months prepartum and incidence rate of mastitis addition an improvement in the reproductive performance in treated cows relative to untreated cows. Therefore, the current study recommends the herdsmen treats their cows with anti-parasites (Ivermectin) during the prepartum period for less mastitis incidence rate and better reproductive efficiency.


2016 ◽  
Vol 46 (11) ◽  
pp. 2018-2023
Author(s):  
Bhárbara Delboni Stuhr ◽  
Isaac Santos Gil ◽  
Tracy Ferreira Lacerda ◽  
Mauricio Gomes Favoreto ◽  
Bárbara Loureiro

ABSTRACT: The equine chorionic gonadotropin (eCG) is a glycoprotein produced in mare endometrial calices. In bovine, it is used in estrus synchronization protocols. However, studies have shown that it is potentially immunogenic and its effect can decrease after repetitive use. This study aimed to evaluate antral follicle dynamics, corpus luteum (CL) and ovulation rate in bos indicus cows submitted to an estrus synchronization protocol in association with eCG for eight times consecutively. Ten cyclical, multiparous, and pasture raised beef cows were divided into two groups: control group (n=5) and eCG group (n=5). In 30 day interval, all animals were synchronized with the same estrogen/progesterone based protocol, totalizing 8 re-synchronizations. Cows in the treatment group received 300IU eCG 48 hours prior to the presumable ovulation. Ultrassound examinations were performed on Day 4 of the protocol (approximately 1.5 days after follicle recruitment) to count antral follicles, on Day 10, to count antral follicles and to measure size of the largest follicle and on Day 18 to measure the diameter of the CL. No difference (P>0.05) between follicular growth and size of the pre-ovulatory follicle was reported between groups. Cows treated with eCG had a larger (P<0.05) CL and increased (P<0.05) ovulation rate (18mm and 92%, respectively) when compared with control group (14.1mm and 80%, respectively). Furthermore, consecutive treatments did not affect CL nor ovulation rates. In conclusion, eCG treatment increased CL size and ovulation rate even after 8 consecutive treatments.


2017 ◽  
Vol 73 (8) ◽  
pp. 479-482 ◽  
Author(s):  
Mushap Kuru ◽  
Osman Sogukpinar ◽  
Mustafa Makav ◽  
Nebi Cetin

This study was aimed at determining the effect of barium selenate injections on the fertility of Pirlak ewes which had been subjected to progesterone-assisted estrus synchronization during the non-breeding season. A total of 150 Pirlak ewes between the ages of 2 and 5 years were used in the study. Sponges containing progesterone were inserted into the vaginas of the ewes for 11 (groups I and III) or 14 days (groups II and IV) for the purpose of estrus synchronization, and 500 IU equine chorionic gonadotropin (eCG) was administered on the day of sponge removal. In addition, barium selenate was injected s.c. immediately before estrus synchronization protocol in groups I and III. After the sponges had been removed, estrus was observed for four days. The ewes were mated with fertile rams. The pregnancy was determined by transrectal ultrasonography 30 days after mating. It was observed that the estrus rate, pregnancy rate, conception rate, lambing rate, twinning rate, and litter size were not statistically different (P > 0.05) between the groups, but estrus onset was different (P < 0.001). In conclusion, barium selenate injections did not affect the fertility parameters before progesterone-assisted synchronization was applied to the Pirlak ewes during the non-breeding season


2015 ◽  
Vol 15 (2) ◽  
pp. 409-418 ◽  
Author(s):  
Juan Carlos Gardón ◽  
Begoña Escribano ◽  
Susana Astiz ◽  
Salvador Ruiz

Abstract An appropriate management of reproduction allows ewes to breed at a more intensive rhythm, more compatible with economic efficiency of farms. Hence, the objectives of the present work were to evaluate a traditional estrus synchronization protocol (based on medroxyprogesterone acetate-impregnated intravaginal sponges combined or not with equine chorionic gonadotropin) on a Merino sheep herd during the breeding season, in order to determine the best working protocol for this breed. A total of 277 sheep, blocked by age (200 ewes and 77 ewe lambs) were randomly allocated to four groups, two of ewes (E and Ee) and two of ewe lambs (L and Le). All females received 60 mg MAP (medroxyprogesterone acetate) sponges on the first day. At the day of removal the animals of groups Ee and Le received 450 IU of eCG (equine chorionic gonadotropin). Mean estrus synchronization rate was 92.06% and similar among groups. Time to estrus was shorter for Ee (46.93±12.44 h) and Le (44.34±10.76 h), when compared to treatments without eCG (60.60±20.46 h and 58.68±17.72 h for E and L, respectively; P<0.05 for both). Pregnancy rates were 71.35 and 72.86% for ewes and ewe lambs, respectively, and similar among groups. Lambing rates were lower in ewes (1.36) than in ewe lambs (1.85) with no significant effect of eCG. The study demonstrates the efficacy of the synchronization method with MAP and the reduction in the time to estrus induced by eCG in Spanish Merino ewes and ewe lambs during the breeding season.


2019 ◽  
Vol 40 (4) ◽  
pp. 1501 ◽  
Author(s):  
Diego Oliveira de Souza ◽  
Monna Lopes de Araújo ◽  
Carmo Emanuel Almeida Biscarde ◽  
Claudinéia da Silva Mendes ◽  
Mariana Alves de Andrade Silva ◽  
...  

The objective of this study was to evaluate the efficacy of delivering reduced doses of hormones via the Bai Hui acupoint in estrus synchronization in goats. A total of 40 goats received intravaginal sponges with medroxyprogesterone acetate for 7 days. The goats were then randomly distributed into 5 treatment: T1 - application of 132.5 ?g of cloprostenol and 300 IU of equine chorionic gonadotropin (eCG), both by intramuscular injection (IM); T2 - application of 39.75 ?g cloprostenol at the Bai Hui acupoint, and 300 IU of eCG by IM; T3 - application of 132.5 ?g of cloprostenol by IM, and 90 IU of eCG at the Bai Hui acupoint; T4 - application of 39.75?g of cloprostenol and 90 UI of eCG, both in Bai Hui and T5 acupuncture: application of 39.75?g of cloprostenol and 90 UI of eCG, both applied in false acupoint. The goats were subjected to an estrus synchronization protocol and monitored for estrus detection, coverage and evaluation of reproductive parameters to detect entry into estrus. The data were subjected to normality tests, followed by appropriate statistical analyses of each variable. There was no significant difference (P > 0.05) in the percentage of animals in estrus (95.00 ± 11.18%), interval between sponge removal and beginning of estrus (49.72 ± 8.93 h), interval between sponge removal and end of estrus (76.84 ± 11.98 h), duration of estrus (27.08 ± 8.68 h), size of the largest follicle (6.82 ± 0.44 mm), interval between sponge removal and ovulation (78.28 ± 10.82 h), time from ovarian onset to estrus (28.52 ± 5.44 h), follicular growth rate (0.86 ± 0.29 mm/day), number of ovulations (1.32 ± 0.23), plasma progesterone concentration at 7 days after ovulation (10.28 ± 1.65 ng.mL-1), and gestation rate at 30 days after the beginning of estrus (75 ± 12.5%). However, the cost of the synchronization protocol per animal was 43.42% lower in treatments 4 and 5 (30% of the doses) than in treatment 1 (100% of the dose). Ovulation and estrus were efficiently synchronized with the use of 39.75 ?g of sodium cloprostenol and 90 UI of eCG, applied at the Bai Hui acupoint or at a false acupoint.


The aim of the study was to investigate the effects of Moringa oleifera aqueous seed extract on live body weight, gonadal and extragonadal dimensions and sperm reserves of Yankasa rams. Twenty five apparently healthy Yankasa rams aged 1-2 years and weighing 19.0 ± 2.1 Kg were used for the study. The rams were randomly selected into five groups: A, B, C, D and E with five rams in each group as treatment and control groups respectively. Groups A - D were given oral dose of Moringa oleifera aqueous seed extract at a dose rate of 1000, 2000, 3000, 4000 (mg/kg), respectively while group E was given 10 ml/kg water orally, daily for five months. Live body weight, gonadal and extragonadal reserves were determined according to standard techniques. The results showed a significant increase in live body weight in the months of April to June among rams treated with different doses of Moringa oleifera aqueous seed extract compared with the control group. The control group showed no significant differences in the body weight, gonadal and extragonadal dimensions and sperm reserves. In conclusion, the treatment of Yankasa rams with Moringa oleifera aqueous seed extract increased live body weight, but had no significant effects on gonadal and extragonadal dimensions and sperm reserves in Yankasa rams. Therefore, it is recommended that M. oleifera aqueous seed extract can be used at doses of 2000mg/kg to 3000mg/kg in Yankasa rams for optimum gain in live body weight.


1993 ◽  
Vol 73 (2) ◽  
pp. 287-293
Author(s):  
S. L. Boyles ◽  
L. J. Johnson ◽  
W. D. Slanger ◽  
B. J. Kreft ◽  
J. D. Kirsch

Twenty-six of 52 heifers with an average liveweight of 318 kg were dewormed by intraruminal injection of oxfendazole and placed in drylot on 31 January. The other 26 animals served as controls. All heifers were pastured starting on 14 June and the treatment heifers were dewormed in the same manner as at the start of the experiment (31 January) on 1 July and 31 July. The groups started out with similar total worm eggs per gram of fecal sample, but the dewormed heifers had fewer counts each month thereafter. The drylot average daily gain of the treated heifers was higher (P = 0.01) and less variable (P = 0.12) and by 29 May these animals were heavier by 11.7 kg (P = 0.10). The treated animals did not gain as fast on pasture (P = 0.20), but the gain continued to be less variable (P = 0.09). Age at puberty was not different. Number of open heifers in the treated group was 4 vs. 15% for the control group (P = 0.18). Deworming by intraruminal injection resulted in faster feedlot gains, more uniform gains during both drylot and pasture, reduced parasites and no abscess at injection sites. Key words: Anthelmintics, oxfendazole, cattle, heifers


1967 ◽  
Vol 7 (24) ◽  
pp. 7 ◽  
Author(s):  
P McInnes ◽  
TJ Grainger ◽  
MD Smith

Data are presented on the recovery and reproductive performance of 2 1/2-year-old maiden Merino ewes after a prolonged period of undernutrition. The 217 sheep had been hand-fed on a submaintenance ration in pen feeding trials at Glenfield, New South Wales. During the seven months of the trials they had lost 6 kg (28 to 22 kg) body weight. They were transported to Condobolin in south-western New South Wales, divided into two treatment groups and run on good quality pastures. One group was joined immediately (May 1959) and again ten months later, and the other group was mated after six months at Condobolin (in October 1959) and again 12 months later. The ewes recovered rapidly. The mean weight of both groups had reached 30 kg within six weeks and 40 kg within six months. In the first year 73 of the 100 May-mated ewes bore lambs, but only 38 of these lambs were weaned. Ewes bearing lambs had a higher body weight at the start of joining and gained more during joining than the barren ewes. At the other three joinings (October 1959, May 1960, October 1960) lambing percentage was from 86-89 and weaning percentage from 62-69-both normal for the district. The proportion of twin lambs (3-6 per cent) was low. Wool weight in 1959 was not affected by time of mating or by pregnancy.


Sign in / Sign up

Export Citation Format

Share Document