Virulence genes in Escherichia coli strains isolated from urine of elderly patients

Biologia ◽  
2017 ◽  
Vol 72 (3) ◽  
Author(s):  
Radka Vargová ◽  
Marta Kmeťová ◽  
Katarína Čurová ◽  
Leonard Siegfried
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Elderly Patients ◽  
Elderly People ◽  
Urinary Tract Infections ◽  
Virulence Genes ◽  
Tract Infections

AbstractUrinary tract infections (UTIs) are one of the most frequent infections in elderly people. The majority of infections is caused by uropathogenic

Molecular Analysis of Uropathogenic E.coli Isolates from Urinary Tract Infections

2019 ◽  
Vol 19 (3) ◽  
pp. 322-326 ◽  
Author(s):  
Hassan Valadbeigi ◽  
Elham Esmaeeli ◽  
Sobhan Ghafourian ◽  
Abbas Maleki ◽  
Nourkhoda Sadeghifard
Keyword(s):  
Escherichia Coli ◽  
Polymerase Chain Reaction ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Multiplex Polymerase Chain Reaction ◽  
Virulence Genes ◽  
Uropathogenic Escherichia Coli ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Tract Infections

Introduction: The aim of the current study was to investigate the prevalence of virulence genes in uropathogenic Escherichia coli (UPEC) isolates in Ilam. Materials and Methods: For this purpose, a total of 80 UPEC isolates were collected for patients with UTIs during a 6 months period. The multiplex polymerase chain reaction (multiplex PCR) was used to detect the papEF, fimH, iucD, hlyA, fyuA, and ompT genes. Results: The prevalence of fimH, papEF, iucD, fyuA, hlyA, hlyA, and ompT genes were 87.5%, 47.5%, 60%, 67.5%, 27.5%, 47.5% and 71.2%, respectively. Among all of the isolates, 27 profiles were obtained. Conclusion: Our findings demonstrated that the most prevalence was found for fimH, and different distribution of virulence genes suggested different ability of pathogenicity.

scholarly journals Prevalence of bacterial urinary tract infections in elderly living in retirement homes in Brazil

2020 ◽  
Vol 30 (Supplement_5) ◽  
Author(s):  
S Tietz Marques ◽  
B Pitz da Silva ◽  
A Silva Mota ◽  
R Marinho de Quadros ◽  
M R de Lima
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Elderly Patients ◽  
Elderly People ◽  
Urinary Tract Infections ◽  
Culture Media ◽  
Biochemical Tests ◽  
Urine Sediment ◽  
Tract Infections

Abstract Background Urinary tract infections are common in humans. Urinalysis is the most widely used test for the diagnosis of urinary tract infection in Brazil. This study aimed to identify bacteria that cause urinary tract infection (UTI) in elderly patients permanently living in three retirement homes in the city of Lages, state of Santa Catarina, Brazil. Methods The study was approved by the Human Research Ethics Committee of University (protocol number 2.922.891). The participants were 93 elderly people, aged between 51 and 94 years. Urine samples were subjected to physical-chemical tests and to direct analysis of cell and bacterial micromorphology. Gram staining was performed on urine sediment. Positive samples were submitted to urine culture using cystine-lactose-electrolyte-deficient agar and MacConkey culture media. Bacterial colonies were identified by Gram-positive bacteria, biochemical tests and for Gram-negative bacteria, biochemical tests of oxidase and Rugai with Lysine (NewProv®). The χ2 test with the FREQ procedure of the SAS 9.2 statistical package was used, adopting p ≤ 0.05 as the level of significance, with a 95% confidence interval (CI). Results The prevalence of bacterial infection was 36.55% with rates of 46.93% in women and 25% in men. There was no positive correlation between urinary tract infections in relation to sex, age group, pH and diaper use (p > 0.05). E. coli appeared in the urine of 20.43% of the elderly, S. aureus in 8,16%; S. saprophyticus in 9,09%, 5,37% with Proteus mirabilis and Klebsiella pneumoniae. Conclusions Collaborative and integrated actions are needed to facilitate the recognition and clinical management of UTI in elderly patients, which are responsibility of Federal Government social programs in partnership with states and municipalities. Key messages Elderly patients need regular monitoring. Elderly people in nursing homes need to be served by government programs.

scholarly journals Antimicrobial Resistance of Uropathogenic Escherichia coli from Elderly Patients at a General Hospital, Argentina

2018 ◽  
Vol 10 (1) ◽  
pp. 79-87 ◽  
Author(s):  
Gastón Delpech ◽  
Natalia García Allende ◽  
Sabina Lissarrague ◽  
Mónica Sparo
Keyword(s):  
Risk Factors ◽  
Escherichia Coli ◽  
Urinary Tract ◽  
Antimicrobial Resistance ◽  
Elderly Patients ◽  
Nalidixic Acid ◽  
Urinary Tract Infections ◽  
E Coli ◽  
Amoxicillin Clavulanate ◽  
Tract Infections

Background:Urinary Tract Infection (UTI) is a common cause of morbidity and mortality in older adults.Objective:To investigate antimicrobial resistance of uropathogenicEscherichia colifrom elderly patients in a General Hospital, Argentina.Method:During the period July 2011-July 2015, patients over 70 years old with urinary tract infections, without urinary catheters and with no antimicrobial therapy the previous week before sampling, were included. Phenotypic characterization was carried out.In vitroqualitative and quantitative antimicrobial resistances were investigated. Antimicrobials assayed: ampicillin, amoxicillin-clavulanate, cefazolin, cefuroxime, cefoxitin, cefotaxime, ceftazidime, cefepime, imipenem, ertapenem, gentamicin, nalidixic acid, ciprofloxacin, trimethoprim-sulfamethoxazole (TMS) and nitrofurantoin. Patients’ medical records were produced, and risk factors were analyzed by multivariate analysis.Results:768 bacterial isolates were identified asE. coli. Resistances to ampicillin (80.5%), nalidixic acid (61.7%), ciprofloxacin (42.8%), TMS (37.6%), amoxicillin-clavulanate (28.6%), cefazolin (21.6%), cefuroxime (20.7%), gentamicin (13.8%), cefotaxime (9.7%), ceftazidime (9.7%), cefepime (8.4%), cefoxitin (3.1%) and nitrofurantoin (2.3%) were observed. Resistance to carbapenems was not expressed. Production of extended spectrum β-lactamases was detected (7.6%) in community acquired (96%) and healthcare associated (4%) isolates. The independent risk factors for urinary infections produced by multi-resistantE. coliwere: diabetes mellitus, recurrent infections, hospitalization during the last year and exposure to β-lactams in the last 3 months.Conclusion:A high prevalence of resistance to β-lactams and to other antimicrobials was observed. Detection of antimicrobial multi-resistant isolates highlights the need of antimicrobial resistance surveillance in elderly patients with urinary tract infections.

Comparison of virulence genes and phylogenetic groups of Escherichia coli isolates from urinary tract infections and normal fecal flora

Gene Reports ◽  
2020 ◽  
Vol 20 ◽  
pp. 100709
Author(s):  
Nooshin Mojaz-Dalfardi ◽  
Davood Kalantar-Neyestanaki ◽  
Zahra Hashemizadeh ◽  
Shahla Mansouri
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Virulence Genes ◽  
Fecal Flora ◽  
Phylogenetic Groups ◽  
Tract Infections

scholarly journals Urinary Excretion and Bactericidal Activities of Gemifloxacin and Ofloxacin after a Single Oral Dose in Healthy Volunteers

2001 ◽  
Vol 45 (12) ◽  
pp. 3524-3530 ◽  
Author(s):  
Christoph K. Naber ◽  
Michaela Hammer ◽  
Martina Kinzig-Schippers ◽  
Christian Sauber ◽  
Fritz Sörgel ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Urinary Tract ◽  
Urinary Excretion ◽  
Enterococcus Faecalis ◽  
Urinary Tract Infections ◽  
Parent Drug ◽  
Oral Dosage ◽  
Tract Infections ◽  
Cumulative Excretion

ABSTRACT In a randomized crossover study, 16 volunteers (8 men, 8 women) received single oral doses of 320 mg of gemifloxacin and 400 mg of ofloxacin on two separate occasions in the fasting state to assess the urinary excretion and urinary bactericidal titers (UBTs) at intervals for up to 144 h. Ofloxacin showed higher concentrations in urine compared with those of gemifloxacin. The median (range) cumulative excretion of gemifloxacin was 29.7% (8.4 to 48.7%) of the parent drug administered, and median (range) cumulative excretion of ofloxacin was 84.3% (46.5 to 95.2%) of the parent drug administered. The UBTs, i.e., the highest twofold dilutions (with antibiotic-free urine as the diluent) of urine that were still bactericidal, were determined for a reference strain and nine uropathogens for which the MICs of gemifloxacin and ofloxacin were as follows:Escherichia coli ATCC 25922, 0.016 and 0.06 μg/ml, respectively; Klebsiella pneumoniae, 0.03 and 0.06 μg/ml, respectively; Proteus mirabilis, 0.125 and 0.125 μg/ml, respectively; Escherichia coli, 0.06 and 0.5 μg/ml, respectively; Pseudomonas aeruginosa, 1 and 4 μg/ml, respectively; Staphylococcus aureus, 0.008 and 0.25 μg/ml, respectively; Enterococcus faecalis, 0.06 and 2 μg/ml, respectively;Staphylococcus aureus, 0.25 and 4 μg/ml, respectively;Enterococcus faecalis, 0.5 and 32 μg/ml, respectively; and Staphylococcus aureus, 2 and 32 μg/ml, respectively. Generally, the UBTs for gram-positive uropathogens were higher for gemifloxacin than for ofloxacin and the UBTs for gram-negative uropathogens were higher for ofloxacin than for gemifloxacin. According to the UBTs, ofloxacin-resistant uropathogens (MICs, ≥4 mg/liter) should also be considered gemifloxacin resistant. Although clinical trials have shown that gemifloxacin is effective for the treatment of uncomplicated urinary tract infections, whether an oral dosage of 320 mg of gemifloxacin once daily is also adequate for the treatment of complicated urinary tract infections has yet to be confirmed.

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