scholarly journals Computerized Reconstruction of Pulpal Blood Vessels Examined under Confocal Microscope

2015 ◽  
Vol 19 (1) ◽  
pp. 43-49
Author(s):  
Anna Digka ◽  
Kleoniki Lyroudia ◽  
Lucie Kubinova ◽  
Georgia Karayannopoulou ◽  
Ioannis Marras ◽  
...  
Keyword(s):  
Blood Vessels ◽  
Cross Sections ◽  
Automatic Segmentation ◽  
Spatial Relationship ◽  
Confocal Microscope ◽  
Confocal Laser ◽  
Computer Assisted ◽  
3 Dimensional ◽  
Dimensional Reconstruction ◽  
And Function

SUMMARYThe purpose of this study was the evaluation of 3 different histological methods for studying pulpal blood vessels in combination with 2 types of confocal microscope and computer assisted 3-dimensional reconstruction. 10 human, healthy, free of restorations or caries teeth that were extracted for orthodontic reasons were used. From these teeth, the pulp tissues of 5 were removed, fixed in formalin solution, dehydrated and embedded in paraffin. Serial cross sections 5μm thick were taken from 3 of the above mentioned pulpal tissues and stained with CD34 according to the immunohistochemical ABC technique, while the rest 2 were stained with CD34 and Cy5 by means of immunofluorescence after serial cross sectioning of 10μm. 5 of the 10 teeth were fixed, decalcified, serial cross sectioned (30μm thickness) and stained with eosin. The physical sections were examined under 2 types of confocal laser microscope. Serial images were taken for each section, alignment of the images was followed and finally 3-dimensional reconstructions of the pulpal vessels were achieved.The combined use of immunofluorescence, confocal microscope and automatic segmentation proved to be a useful method for the detailed study of pulpal vasculature. The above method provides deep knowledge of the form and spatial relationship even of the smallest pulpal blood vessels with neighbouring structures like odontoblasts, which are essential for the fully understanding of their role and function within the dental pulp.

scholarly journals Segmentation and Classification of Heart Angiographic Images Using Machine Learning Techniques

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Abdullah ◽  
Muhammad Hameed Siddiqi ◽  
Yousef Salamah Alhwaiti ◽  
Ibrahim Alrashdi ◽  
Amjad Ali ◽  
...  
Keyword(s):  
Blood Vessels ◽  
Human Heart ◽  
Automatic Segmentation ◽  
Ensemble Classification ◽  
Machine Learning Techniques ◽  
Computer Assisted ◽  
Time Saving ◽  
Significant Information ◽  
Heart Blood

Heart angiography is a test in which the concerned medical specialist identifies the abnormality in heart vessels. This type of diagnosis takes a lot of time by the concerned physician. In our proposed method, we segmented the interested regions of heart vessels and then classified. Segmentation and classification of heart angiography provides significant information for the physician as well as patient. Contradictorily, in the mention domain of heart angiography, the charge is prone to error, phase overwhelming, and thought-provoking task for the physician (heart specialist). An automatic segmentation and classification of heart blood vessels descriptions can improve the truthfulness and speed up the finding of heart illnesses. In this work, we recommend a computer-assisted conclusion arrangement for the localization of human heart blood vessels within heart angiographic imageries by using multiclass ensemble classification mechanism. In the proposed work, the heart blood vessels will be first segmented, and the various features according to accuracy have been extracted. Low-level features such as texture, statistical, and geometrical features were extracted in human heart blood vessels. At last, in the proposed framework, heart blood vessels have been categorized in their four respective classes including normal, block, narrow, and blood flow-reduced vessels. The proposed approach has achieved best result which provides very useful, easy, accurate, and time-saving environment to cardiologists for the diagnosis of heart-related diseases.

Collagen fibril bundles: a branching assembly unit in tendon morphogenesis

Development ◽  
1989 ◽  
Vol 107 (3) ◽  
pp. 437-443 ◽  
Author(s):  
D.E. Birk ◽  
J.F. Southern ◽  
E.I. Zycband ◽  
J.T. Fallon ◽  
R.L. Trelstad
Keyword(s):  
Chick Embryo ◽  
Collagen Fibril ◽  
Three Dimensional ◽  
Computer Assisted ◽  
Intimate Association ◽  
Rapid Changes ◽  
Dimensional Reconstruction ◽  
Assembly Unit ◽  
And Function ◽  
Structure Stabilization

The assembly, deposition and organization of collagen fibril bundles and their composite fibrils were studied during morphogenesis of the chick embryo tendon using electron microscopy, serial sections and computer-assisted three-dimensional reconstruction techniques. The 14-day chick embryo is a stage when tendon architecture is being established and rapid changes in the mechanical properties occur between days 14 and 17 of development. Tendon matrix structure develops from discrete subunits, bundles of collagen fibrils. The bundles branch; undergo a gradual rotation over several micrometers; are intimately associated with the cellular elements of the developing tendon; and form arborizing networks within and among fascicles. The organization of discrete fibril segments into bundles, during the establishment of tendon architecture and function, where the segmental fibrillar components could interact with the interfibrillar matrix as well as with adjacent fibrils would contribute to the stabilization of this structure. The observed gradual rotation of the bundles would serve to stabilize the immature bundle through the physical twining of the composite fibrils while the extensive branching of the bundles observed at 14-days of development and their intimate association with the cellular elements would provide a higher order of structure stabilization.

scholarly journals Significance of the spatial reconstruction based on mathematical modeling in the surgical treatment of giant intracranial aneurysms

2006 ◽  
Vol 63 (1) ◽  
pp. 65-68 ◽  
Author(s):  
Igor Nikolic ◽  
Mirjana Nagulic ◽  
Vaso Antunovic
Keyword(s):  
Blood Vessels ◽  
Intracranial Aneurysms ◽  
Giant Aneurysm ◽  
Diagnostic Procedures ◽  
Left Middle Cerebral Artery ◽  
Conventional Angiography ◽  
3 Dimensional ◽  
Giant Intracranial Aneurysms ◽  
Spatial Reconstruction ◽  
Dimensional Reconstruction

Background. The use of computer models for the 3- dimensional reconstruction could be a reliable method to overcome technical imperfections of diagnostic procedures for the microsurgical operation of giant intracranial aneurysms. Case report. We presented a case of successfully operated 52-year-old woman with giant intracranial aneurysm, in which the computer 3-dimensional reconstruction of blood vessels and the aneurysmal neck had been decisive for making the diagnosis. The model for 3- dimensional reconstruction of blood vessels was based on the two 2-dimensional projections of the conventional angiography. Standard neuroradiologic diagnostic procedures showed a giant aneurysm on the left middle cerebral artery, but the conventional subtraction and CT angiography did not reveal enough information. By the use of a personal computer, we performed a 3-dimensional spatial reconstruction of the left carotid artery to visualize the neck of aneurysm and its supplying blood vessels. Conclusion. The 3-dimensional spatial reconstruction of the cerebral vessels of a giant aneurysm based on the conventional angiography could be useful for planning the surgical procedure.

scholarly journals Three dimensional reconstruction of energy stores for jumping in planthoppers and froghoppers from confocal laser scanning microscopy

eLife ◽  
2017 ◽  
Vol 6 ◽  
Author(s):  
Igor Siwanowicz ◽  
Malcolm Burrows
Keyword(s):  
Laser Scanning ◽  
Methodological Approach ◽  
Three Dimensional ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Stored Energy ◽  
Joint Angles ◽  
Energy Stores ◽  
Dimensional Reconstruction ◽  
And Function

Jumping in planthopper and froghopper insects is propelled by a catapult-like mechanism requiring mechanical storage of energy and its quick release to accelerate the hind legs rapidly. To understand the functional biomechanics involved in these challenging movements, the internal skeleton, tendons and muscles involved were reconstructed in 3-D from confocal scans in unprecedented detail. Energy to power jumping was generated by slow contractions of hind leg depressor muscles and then stored by bending specialised elements of the thoracic skeleton that are composites of the rubbery protein resilin sandwiched between layers of harder cuticle with air-filled tunnels reducing mass. The images showed that the lever arm of the power-producing muscle changed in magnitude during jumping, but at all joint angles would cause depression, suggesting a mechanism by which the stored energy is released. This methodological approach illuminates how miniaturized components interact and function in complex and rapid movements of small animals.

Three-Dimensional Reconstruction Using Stereo Pairs from Serial Thick Sections

1977 ◽  
Vol 35 ◽  
pp. 562-563
Author(s):  
Robert Glaeser ◽  
Thomas Bauer ◽  
David Grano
Keyword(s):  
Three Dimensional ◽  
Dimensional Structure ◽  
Serial Sections ◽  
Thin Sections ◽  
3 Dimensional ◽  
Transmission Electron ◽  
Freeze Dried ◽  
Dimensional Reconstruction ◽  
Stereo Imagery ◽  
Whole Mounts

In transmission electron microscopy, the 3-dimensional structure of an object is usually obtained in one of two ways. For objects which can be included in one specimen, as for example with elements included in freeze- dried whole mounts and examined with a high voltage microscope, stereo pairs can be obtained which exhibit the 3-D structure of the element. For objects which can not be included in one specimen, the 3-D shape is obtained by reconstruction from serial sections. However, without stereo imagery, only detail which remains constant within the thickness of the section can be used in the reconstruction; consequently, the choice is between a low resolution reconstruction using a few thick sections and a better resolution reconstruction using many thin sections, generally a tedious chore. This paper describes an approach to 3-D reconstruction which uses stereo images of serial thick sections to reconstruct an object including detail which changes within the depth of an individual thick section.

Structural preservation and absolute contrast of catalase crystal sections prepared with tannic acid

1983 ◽  
Vol 41 ◽  
pp. 448-449
Author(s):  
C.W. Akey ◽  
M. Szalay ◽  
S.J. Edelstein
Keyword(s):  
Tannic Acid ◽  
Beef Heart ◽  
X Rays ◽  
Screw Axis ◽  
General Applicability ◽  
Thin Sections ◽  
Beef Liver ◽  
3 Dimensional ◽  
Dimensional Reconstruction ◽  
Structural Preservation

Three methods of obtaining 20 Å resolution in sectioned protein crystals have recently been described. They include tannic acid fixation, low temperature embedding and grid sectioning. To be useful for 3-dimensional reconstruction thin sections must possess suitable resolution, structural fidelity and a known contrast. Tannic acid fixation appears to satisfy the above criteria based on studies of crystals of Pseudomonas cytochrome oxidase, orthorhombic beef liver catalase and beef heart F1-ATPase. In order to develop methods with general applicability, we have concentrated our efforts on a trigonal modification of catalase which routinely demonstrated a resolution of 40 Å. The catalase system is particularly useful since a comparison with the structure recently solved with x-rays will permit evaluation of the accuracy of 3-D reconstructions of sectioned crystals.Initially, we re-evaluated the packing of trigonal catalase crystals studied by Longley. Images of the (001) plane are of particular interest since they give a projection down the 31-screw axis in space group P3121. Images obtained by the method of Longley or by tannic acid fixation are negatively contrasted since control experiments with orthorhombic catalase plates yield negatively stained specimens with conditions used for the larger trigonal crystals.

Computer Assisted Image Reconstruction of Oligomer Structure

1976 ◽  
Vol 34 ◽  
pp. 472-473
Author(s):  
A.M. Jones ◽  
A. Max Fiskin
Keyword(s):  
Three Dimensional ◽  
Depth Of Field ◽  
Computer Assisted ◽  
Projection Data ◽  
Two Dimensional ◽  
Single Particles ◽  
Dimensional Reconstruction ◽  
Computer Assisted Image ◽  
The Fourier Transform ◽  
Space Approach

If the tilt of a specimen can be varied either by the strategy of observing identical particles orientated randomly or by use of a eucentric goniometer stage, three dimensional reconstruction procedures are available (l). If the specimens, such as small protein aggregates, lack periodicity, direct space methods compete favorably in ease of implementation with reconstruction by the Fourier (transform) space approach (2). Regardless of method, reconstruction is possible because useful specimen thicknesses are always much less than the depth of field in an electron microscope. Thus electron images record the amount of stain in columns of the object normal to the recording plates. For single particles, practical considerations dictate that the specimen be tilted precisely about a single axis. In so doing a reconstructed image is achieved serially from two-dimensional sections which in turn are generated by a series of back-to-front lines of projection data.

Some quantitative applications of vascular corrosion casting

1992 ◽  
Vol 50 (1) ◽  
pp. 738-739
Author(s):  
Fred E. Hossler
Keyword(s):  
Blood Vessels ◽  
Nuclear Orientation ◽  
Three Dimensional ◽  
Surrounding Tissue ◽  
Confocal Laser ◽  
Corrosion Casting ◽  
Venous Valve ◽  
Casting Technique ◽  
Low Viscosity ◽  
Quantitative Measurements

Preparation of replicas of the complex arrangement of blood vessels in various organs and tissues has been accomplished by infusing low viscosity resins into the vasculature. Subsequent removal of the surrounding tissue by maceration leaves a model of the intricate three-dimensional anatomy of the blood vessels of the tissue not obtainable by any other procedure. When applied with care, the vascular corrosion casting technique can reveal fine details of the microvasculature including endothelial nuclear orientation and distribution (Fig. 1), locations of arteriolar sphincters (Fig. 2), venous valve anatomy (Fig. 3), and vessel size, density, and branching patterns. Because casts faithfully replicate tissue vasculature, they can be used for quantitative measurements of that vasculature. The purpose of this report is to summarize and highlight some quantitative applications of vascular corrosion casting. In each example, casts were prepared by infusing Mercox, a methyl-methacrylate resin, and macerating the tissue with 20% KOH. Casts were either mounted for conventional scanning electron microscopy, or sliced for viewing with a confocal laser microscope.

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