scholarly journals Interactive effects of temperature and light on reattachment success in the brown alga Fucus radicans

2019 ◽  
Vol 62 (1) ◽  
pp. 43-50
Author(s):  
Ellen Schagerström ◽  
Tiina Salo

Abstract Fucus radicans is an endemic habitat-forming brown macroalga in the Baltic Sea that commonly complements its sexual reproduction with asexual reproduction. Asexual reproduction in F. radicans takes place through formation of adventitious branches (hereafter fragments), but the exact mechanisms behind it remain unknown. We assessed experimentally the importance of two environmental factors determining the re-attachment success of F. radicans fragments. By combining different light conditions (daylength and irradiance; high or low light) and water temperature (+14°C and +4°C), we mimicked ambient light and temperature conditions of winter, spring/autumn and summer for F. radicans. Fragments were able to re-attach in all tested conditions. Temperature and light had an interactive impact on re-attachment: the combination of high temperature and high light level resulted in the highest re-attachment success, while light level had no effects on re-attachment success in cooler water temperature and the re-attachment success in high temperature under low light levels was very low. The results suggest that rhizoid formation, and thus re-attachment success, may depend on the net primary production (metabolic balance) of the fragment. However, whether the re-attachment and asexual reproduction success simply depends on photosynthetic capacity warrants further mechanistic studies. Understanding the mechanisms of asexual reproduction in F. radicans is important in order to assess the dispersal capacity of this foundation species.

2022 ◽  
Vol 2149 (1) ◽  
pp. 012016
Author(s):  
Z F Wu ◽  
L Li ◽  
C H Dai ◽  
Y F Wang ◽  
Q T Cheng ◽  
...  

Abstract Low light level (LLL) calibration becomes more and more important since the rapid growth of remote sensing. The spectral radiance at normal higher light levels can be calibrated with good accuracy, while LLL spectral radiance cannot. If an adjustable light source can be designed at nearly constant correlated color temperature (CCT) covering several orders of magnitude, low light level spectral radiance can be obtained with the help of a photodetector. Whether or not the spectral distribution of an integrating sphere based light source is nearly constant is investigated. By adjusting the diameter of the variable aperture between the integrating sphere and tungsten lamp, the spectral radiance can be varied over 6 orders of magnitude. However, the relative spectrum in the red region increases notably when the spectral radiance is decreased to 1/100000. If the spectral radiance is decreased further, the spectral difference can be more than 300% and CCT decreases more than 250 K. By using baffles and another integrating sphere, low light level radiation source at nearly constant spectral distribution is obtained. The variation of CCT is less than 50 K over 6 orders of magnitude.


1980 ◽  
Vol 9 (1) ◽  
pp. 37-44
Author(s):  
John B. Bunch

The purpose of this experiment was to evaluate the ability of photography students to focus a camera accurately under two levels of illumination (high/low) with three types of focusing screens (plain groundglass, split-image and microprism). The participants were required to focus each screen three times under each light level; scores were focus errors in feet. A 2 × 3 MANOVA design was used, and post hoc comparisons were made for plain vs. split-image, plain vs. microprism, and plain vs. microprism and split-image combined. There was significantly less focus error (p < .05) under the low illumination level, but no significant difference between screen types across both light levels. The screen type X light level interaction was much higher than the p .05 required in this study. Plain groundglass produced the least overall error; microprism yielded the least error under low illumination and the highest error under high illumination; and split-image was the most accurate under high illumination and the least accurate for the low light level condition.


HortScience ◽  
1997 ◽  
Vol 32 (3) ◽  
pp. 491D-491
Author(s):  
Jan M. Kossowski ◽  
David W. Wolfe

Long- and short-term physiological responses of pak choi (Chinese cabbage, Brassica campestris cv. `Hypro') to elevated CO2 and light environments were evaluated in the series of growth chamber experiments. Plants were grown hydroponically (Nutrient Film Technique) at 25/18°C (day/night) temperature, a 16-h photoperiod, and at three CO2 levels (350, 700, 1400 ppm) and two light levels (200 and 400 μmol·m–2·s–1 PPFD). Relative to 350-ppm CO2 treatment, the final total plant dry mass in low light increased by 37% and 38% at 700 and 1400 ppm CO2, respectively. In high light the increase was 7% and 13% at 700 and 1400 ppm CO2, respectively. Light response curves showed a positive CO2 effect on light compensation point, a slight increase in quantum yield and increase in maximum Pn rates at elevated CO2. Carbon dioxide response curves (measured at saturating PPFD of 1600 μmol·m–2·s–1) showed no effect of growth light treatment on the CO2 compensation point, but a 20% to 30% higher maximum Pn rate at saturating CO2 in plants grown at the higher light level. Overall, the highest Pn rates and the highest plant dry mass at final harvest were found in plants grown at the 400 μmol·m–2·s–1 PPFD and 1400 ppm CO2. Relative beneficial CO2 effects, however, were the most pronounced in low light conditions.


Author(s):  
G. W. Potts ◽  
J. W. Wood ◽  
J. M. Edwards

Television cameras and video equipment are well established as tools in many areas of marine research and have been reviewed by Barnes (1963), Myrberg (1973), Harris (1980), and Holme (1984, 1985). The TV camera is usually mounted on a remote sledge (Machan & Fedra, 1975; Holme & Barrett, 1977) or submersible, often with arrays of lights, and lacks the manoeuvrability that is necessary for many types of detailed survey and behavioural recording. Commercial and industrial uses also demand high-resolution equipment that is often contained in bulky underwater cases capable of operating at great depths and well below that possible by the conventional SCUBA diver. It is not unusual for the equipment to be used in conjunction with external lighting units where natural light levels are too low for the use of ambient light, and is dependent on an external power supply normally situated in the surface support vessel or within a submersible (Sisman, 1982).


2020 ◽  
Author(s):  
John K. Keep ◽  
Jabin R. Watson ◽  
Rebecca L. Cramp ◽  
Matthew J. Jones ◽  
Matthew A. Gordos ◽  
...  

AbstractInadequately designed culverts are known to pose hydraulic barriers to fish passage, but they may also be behavioural barriers if they adversely affect light levels within them. To test this, we performed a choice experiment and quantified the amount of time individuals of four Australian fish species spent in darkened and illuminated areas of an experimental swimming fume. Behavioural responses were reflective of the species’ diel activity patterns; diurnal species preferred illuminated regions, while nocturnal species preferred the darkened region. We then determined a threshold light level of only ~100-200 lux (c.f. midday sunlight ~100,000 lux) was required to overcome the behavioural barrier in ~ 70% of the diurnal fish tested. Placing these threshold values into field context, 100% of culverts sampled recorded inadequate light levels. Attention is required to better understand the impacts of low light levels in culverts on fish passage and to prioritise restoration.


Plants ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1783
Author(s):  
Louis John Irving ◽  
Sayuki Mori

Plants allocate biomass to above- and below-ground organs in response to environmental conditions. While the broad patterns are well-understood, the mechanisms by which plants allocate new growth remain unclear. Modeling approaches to biomass allocation broadly split into functional equilibrium type models and more mechanistically based transport resistance type models. We grew Poa annua plants in split root boxes under high and low light levels, high and low N supplies, with N supplied equally or unequally. Our data suggest that light level had the strongest effect on root mass, with N level being more important in controlling shoot mass. Allocation of growth within the root system was compatible with phloem partitioning models. The root mass fraction was affected by both light and N levels, although within light levels the changes were primarily due to changes in shoot growth, with root mass remaining relatively invariant. Under low light conditions, plants exhibited increased specific leaf area, presumably to compensate for low light levels. In a follow-up experiment, we showed that differential root growth could be suppressed by defoliation under low light conditions. Our data were more compatible with transport resistance type models.


Weed Science ◽  
1988 ◽  
Vol 36 (2) ◽  
pp. 137-140 ◽  
Author(s):  
John B. Masiunas ◽  
Stephen C. Weller

The response of potato (Solanum tuberosumL. 'Superior’) to glyphosate [N-(phosphonomethyl)glycine] at rates between 0.28 and 2.24 kg ae/ha was determined in four environments: 24/13 or 13/4 C temperature regimes with light levels of 650 ± 53 or 320 ± 45 μE · m−2· S−1. Light level did not affect phytotoxicity ratings or shoot fresh weight following glyphosate application. Greater phytotoxicity and reduced fresh weight accumulation were observed from glyphosate at rates above 0.56 kg/ha in a high-temperature regime than for comparable plants grown at low temperature. Studies of14C-glyphosate uptake and translocation indicated that the differences in phytotoxicity were due to less glyphosate absorption at low temperature and not a shift in distribution patterns within the plant.


2003 ◽  
Vol 54 (4) ◽  
pp. 333 ◽  
Author(s):  
Kerstin A. Fritsches ◽  
N. Justin Marshall ◽  
Eric J. Warrant

The large eyes and well-developed visual system of billfishes suggest that vision is an important sense for the detection and interception of prey and lures. Investigations of visual abilities in these large pelagic fishes are difficult, however anatomical studies of billfish eyes and retinas allow prediction of a number of visual capabilities. From the density of ganglion cells in the blue marlin (Makaira nigricans) retina, visual acuities of less than 10 cycles per degree were derived, a surprisingly low visual resolution given the absolute size of the marlin eye. Cone photoreceptors, on the other hand, were present in high densities, resulting in a presumed summation of cones to ganglion cells at a ratio of 40 : 1, even in the area of best vision. The optical sensitivity of the marlin eye was high owing to the large dimensions of the cone photoreceptors. These results indicate that the marlin eye is specifically adapted to cope with the low light levels encountered during diving. Since the marlin is likely to use its vision at depth, it is suggested that this line of research could help estimate the limits of vertical distribution based on light level.


Author(s):  
G.Y. Fan ◽  
J.M. Cowley

In recent developments, the ASU HB5 has been modified so that the timing, positioning, and scanning of the finely focused electron probe can be entirely controlled by a host computer. This made the asynchronized handshake possible between the HB5 STEM and the image processing system which consists of host computer (PDP 11/34), DeAnza image processor (IP 5000) which is interfaced with a low-light level TV camera, array processor (AP 400) and various peripheral devices. This greatly facilitates the pattern recognition technique initiated by Monosmith and Cowley. Software called NANHB5 is under development which, instead of employing a set of photo-diodes to detect strong spots on a TV screen, uses various software techniques including on-line fast Fourier transform (FFT) to recognize patterns of greater complexity, taking advantage of the sophistication of our image processing system and the flexibility of computer software.


Author(s):  
W. Lin ◽  
J. Gregorio ◽  
T.J. Holmes ◽  
D. H. Szarowski ◽  
J.N. Turner

A low-light level video microscope with long working distance objective lenses has been built as part of our integrated three-dimensional (3-D) light microscopy workstation (Fig. 1). It allows the observation of living specimens under sufficiently low light illumination that no significant photobleaching or alternation of specimen physiology is produced. The improved image quality, depth discrimination and 3-D reconstruction provides a versatile intermediate resolution system that replaces the commonly used dissection microscope for initial image recording and positioning of microelectrodes for neurobiology. A 3-D image is displayed on-line to guide the execution of complex experiments. An image composed of 40 optical sections requires 7 minutes to process and display a stereo pair.The low-light level video microscope utilizes long working distance objective lenses from Mitutoyo (10X, 0.28NA, 37 mm working distance; 20X, 0.42NA, 20 mm working distance; 50X, 0.42NA, 20 mm working distance). They provide enough working distance to allow the placement of microelectrodes in the specimen.


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