scholarly journals Experimental and numerical investigations of fluid flow for optimized in vitro stem cell loading in xenografts

2017 ◽  
Vol 3 (2) ◽  
pp. 799-802
Author(s):  
Robert Ott ◽  
Carolin Wüstenhagen ◽  
Heiner Martin ◽  
Michael Stiehm ◽  
Wolfram Schmidt ◽  
...  
Keyword(s):  
Stem Cell ◽  
Fluid Flow ◽  
Pressure Gradient ◽  
Cfd Simulation ◽  
Reynolds Numbers ◽  
Distribution Analysis ◽  
Cell Distribution ◽  
Flow Conditions ◽  
Micro Computed Tomography ◽  
Numerical Investigations

AbstractIn dentofacial surgery, augmentation procedures employing xenografts have become a reliable treatment. Recent studies, however, have shown significant enhance-ments of the in vivo bone tissue augmentation using mesenchymal stem cells loaded into bone grafts. We conducted experimental and numerical investigations in flow perfusion systems to determine flow conditions which allow for homogenous stem cell distribution in BioOss Block (Geistlich Pharma AG, Switzerland) xenografts. Pressure gradient-velocity characteristics and flow distributions were investigated experimentally and numerically at steady state flow conditions with Reynolds numbers (Re) ranging from 0.01 ≤ Re ≤ 0.40. Distilled water at 20°C with a dynamic viscosity of 1.002 mPa.s and a density of 998 kg/m3 was used. The geometry utilized in three-dimensional computa-tional fluid dynamics (CFD) simulation was obtained by means of micro-computed tomography (μCT). Results of CFD analysis are in good accordance with experimental data. The comparison of the pressure gradient-velocity characteris-tics for experimental and numerical data yields a relative error of 3.6%. According to Darcy’s law for creeping fluid flow the experimentally determined permeability is 2.55.10-9 m2. Moreover, numerical flow distribution analysis shows an increasingly heterogenic streamline distribution for increasing Reynolds numbers. Experimentally validated CFD simulations introduced in this study provide a tool to assess optimal flow conditions for a homogenous stem cell distribution in perfusion flow systems.

scholarly journals Experimental and numerical investigations of fluid flow in bioreactors for optimized in vitro stem cell loading in xenografts

2018 ◽  
Vol 4 (1) ◽  
pp. 423-427
Author(s):  
Robert Ott ◽  
Carolin Wüstenhagen ◽  
Michael Stiehm ◽  
Klaus-Peter Schmitz ◽  
Stefan Siewert ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cfd Simulation ◽  
Healing Process ◽  
Three Dimensional ◽  
Flow Conditions ◽  
Micro Computed Tomography ◽  
3D Scaffold ◽  
Numerical Investigations

AbstractIn tissue engineering and regenerative medicine mesenchymal stem cells (MSC) are widely used to replace and restore the function of dysfunctional or missing tissue. Recent studies have shown significant enhancements of the in vivo healing process following dentofacial bone augmentation procedures employing stem cell-loaded xenografts. We conducted experimental and numerical investigations in perfusion flow bioreactor-xenograft-systems to identify flow conditions as well as bioreactor design features that allow for homogeneous MSC-distribution in Geistlich Bio- Oss Block xenografts. Pressure gradient - velocity characteristics and flow distributions were investigated experimentally and numerically for two bioreactor designs at steady-state flow conditions with Reynolds numbers (Re) ranging from 0.01 ≤ Re ≤ 0.32. Distilled water at 20°C with a dynamic viscosity of 1.002 mPa∙s and a density of 998 kg/m3 was used. The geometry of the xenograft utilized in three-dimensional computational fluid dynamics (CFD) simulation was obtained by means of micro-computed tomography (μCT) at an isotropic spatial resolution of 9.5 μm. The permeability values calculated from the experimental data are in good accordance with the numerical results. The investigations showed that the increase of the inflow- and outflow-area diameter, as well as the decrease of the volumetric flow rate, result in a decreasing heterogeneity of the flow distribution within the xenograft. The calculated wall shear stress rates in the three-dimensional (3D) scaffold range from 1∙10-12Pa ≤ τ ≤ 0.2 Pa. Experimentally validated CFD simulations introduced in this study provide an applicable tool to assess optimal flow conditions for homogeneous MSC distribution in bioreactor-xenograft-systems.

A Comprehensive Study of Asymmetric Micro-Droplet Splitting in T-Junction

2019 ◽  
Author(s):  
Way Lee Cheng ◽  
Reza Sadr ◽  
Arum Han
Keyword(s):  
Pressure Gradient ◽  
Cfd Simulation ◽  
General Criterion ◽  
Flow Conditions ◽  
Functional Feature ◽  
Splitting Ratio ◽  
Junction Geometry ◽  
Break Up ◽  
Gradient Ratio ◽  
Underlying Mechanisms

Abstract Splitting a single droplet into two unequal portions using a microfluidic T-junction has been an important functional feature of many modern lab-on-a-chip devices. A recent study introduced a general criterion for asymmetric droplet break-up in the range of intermediate Capillary numbers. The current work attempts to analyze, in more details, the different underlying mechanisms governing the asymmetric break-up process. In particular, this work focuses on the relationship between the break-up mechanism versus the splitting ratio of the daughter droplets. CFD simulation is used to closely monitor the effect of different fluid properties on the evolution of droplet break-up process. The splitting ratio under different flow conditions is characterized. Four mechanisms for primary droplet break-up are defined as follows: break-up with permanent obstruction, unstable break-up, breakup with tunnels and non-breakup. In particular, the main focus of this study is on the unstable break-up mechanisms where is very likely results to a much-deviated splitting ratio. Typically, yet unexpectedly, the resulting splitting ratio is often larger than the pressure gradient ratio in the T-junction. However, the two ratios are approximately equals to each other under a limited set of flow conditions. It has been observed that the splitting ratio could be more than double the pressure gradient ratio of the T-junction. The break-up is observed to be in the permanent obstruction mode if the splitting ratio is about the same magnitude as the pressure gradient ratio. The effects of the T-junction geometry on the break-up will also be examined.

Investigations on Fluid Flow and Heat Transfer Performances Within a Real Turbine Blade Channel

2014 ◽  
Author(s):  
Zhaoqing Ke ◽  
Jian Pu ◽  
Jianhua Wang ◽  
Lei Wang ◽  
Zhiqiang Zhang ◽  
...  
Keyword(s):  
Heat Transfer ◽  
Reynolds Number ◽  
Nusselt Number ◽  
Fluid Flow ◽  
Secondary Flow ◽  
Reynolds Numbers ◽  
Numerical Investigations ◽  
Serpentine Channel ◽  
Flow And Heat Transfer ◽  
Working Medium

The characteristics of fluid flow and heat transfer within a smooth three-pass channel of a real low pressure (LP) turbine blade have been investigated through experimental and numerical approaches. The serpentine channel consists of two inlet passes, two dividing walls, two 180 degree bends, twenty-five exits at the trailing edge, and two exits at the blade tip. In the experiments, purified water was used as working medium, the secondary flow patterns at five cross-sections were captured by a particle image velocimetry (PIV) system, the inlet Reynolds number was controlled by a turbine flow meter, and the mass flow rate ejected from each exit was measured by rotameters. Using the commercial software ANSYS CFX 13.0, numerical investigations were carried out. The practicability of four turbulence models, the SSG RSM, SST k-ω, RNG k-ε and standard k-ε models, were estimated. Through qualitative and quantitative comparisons of the secondary flow patterns, local velocity variation trends and mass flow rates between the experimental data and numerical results, the SSG RSM was selected as the most appropriate model in the following numerical investigations. Using ideal gas as working medium, the impacts of Reynolds numbers and rotation numbers on the heat transfer performances were numerically investigated. The numerical results predicted three interesting phenomena: 1) The locally averaged Nusselt number increases generally with the inlet Reynolds numbers. However, the increasing amplitude is significantly different from the correlation suggested by Dittus-Boelter, Nuo = 0.023Re0.8Pr0.4. The effect of the Reynolds number on the Nusselt number is substantially enhanced due to the serpentine channel design with two 180 degree-bends. The enhancement amplitude is described by two fitted coefficients based on Dittus-Boelter correlation. 2) Under a rotation condition, in the 1st and 3rd passes, the enhancement amplitude of the average Nusselt number on the pressure side (PS) is more significant than that on the suction side (SS), whereas in the 2nd pass, the enhancement amplitude on the PS is lower than that on the SS. 3) In the 3rd pass, a higher rotation number leads to a more uniform distribution of the local Nusselt number along the streamwise direction on both the PS and SS.

Experimental and numerical investigations of fluid flow in a hydrocyclone without an air core

CIM Journal ◽  
2017 ◽  
Vol 8 (1) ◽  
Author(s):  
E. Kucukal ◽  
J. R. Kadambi ◽  
J. Furlan ◽  
R. Visintainer
Keyword(s):  
Fluid Flow ◽  
Numerical Investigations ◽  
Air Core

scholarly journals Emergent synchronous beating behavior in spontaneous beating cardiomyocyte clusters

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Kazufumi Sakamoto ◽  
Yoshitsune Hondo ◽  
Naoki Takahashi ◽  
Yuhei Tanaka ◽  
Rikuto Sekine ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Human Embryonic Stem Cell ◽  
Single Cells ◽  
Embryonic Stem ◽  
Kuramoto Model ◽  
Distribution Analysis ◽  
Cell Clusters ◽  
Overdrive Suppression ◽  
The Kuramoto Model

AbstractWe investigated the dominant rule determining synchronization of beating intervals of cardiomyocytes after the clustering of mouse primary and human embryonic-stem-cell (hES)-derived cardiomyocytes. Cardiomyocyte clusters were formed in concave agarose cultivation chambers and their beating intervals were compared with those of dispersed isolated single cells. Distribution analysis revealed that the clusters’ synchronized interbeat intervals (IBIs) were longer than the majority of those of isolated single cells, which is against the conventional faster firing regulation or “overdrive suppression.” IBI distribution of the isolated individual cardiomyocytes acquired from the beating clusters also confirmed that the clusters’ IBI was longer than those of the majority of constituent cardiomyocytes. In the complementary experiment in which cell clusters were connected together and then separated again, two cardiomyocyte clusters having different IBIs were attached and synchronized to the longer IBIs than those of the two clusters’ original IBIs, and recovered to shorter IBIs after their separation. This is not only against overdrive suppression but also mathematical synchronization models, such as the Kuramoto model, in which synchronized beating becomes intermediate between the two clusters’ IBIs. These results suggest that emergent slower synchronous beating occurred in homogeneous cardiomyocyte clusters as a community effect of spontaneously beating cells.

scholarly journals The Profile Drag of Yawed Wings of Infinite Span

1951 ◽  
Vol 3 (3) ◽  
pp. 211-229 ◽  
Author(s):  
A.D. Young ◽  
T.B. Booth
Keyword(s):  
Boundary Layer ◽  
Pressure Gradient ◽  
Drag Coefficient ◽  
Experimental Evidence ◽  
Flat Plate ◽  
Transition Point ◽  
Reynolds Numbers ◽  
External Pressure ◽  
Basic Assumptions ◽  
Angle Of Yaw

SummaryA method is developed for calculating the profile drag of a yawed wing of infinite span, based on the assumption that the form of the spanwise distribution of velocity in the boundary layer, whether laminar or turbulent, is insensitive to the chordwise pressure distribution. The form is assumed to be the same as that accepted for the boundary layer on an unyawed plate with zero external pressure gradient. Experimental evidence indicates that these assumptions are reasonable in this context. The method is applied to a flat plate and the N.A.C.A. 64-012 section at zero incidence for a range of Reynolds numbers between 106 and 108, angles of yaw up to 45°, and a range of transition point positions. It is shown that the drag coefficients of a flat plate varies with yaw as cos½ Λ (where Λ is the angle of yaw) if the boundary layer is completely laminar, and it varies as if the boundary layer is completely turbulent. The drag coefficient of the N.A.C.A. 64-012 section, however, varies closely as cos½ Λ for transition point positions between 0 and 0.5 c. Further calculations on wing sections of other shapes and thicknesses and more detailed experimental checks of the basic assumptions at higher Reynolds numbers are desirable.

CFD Simulation of Fluid Flow Through Porous Media: Application to Decomposed Gases Flow Through Foam Pattern in Lost Foam Casting

Materials ◽  
2003 ◽  
Author(s):  
Sayavur I. Bakhtiyarov ◽  
Ruel A. Overfelt
Keyword(s):  
Cfd Simulation ◽  
Reynolds Numbers ◽  
Phase Flow ◽  
Flow Through Porous Media ◽  
Foam Material ◽  
Element Analysis ◽  
Wide Range ◽  
Flow Through ◽  
Foam Pattern ◽  
Lost Foam

Numerical simulation of decomposed gases through foam pattern was conducted using finite element analysis. A new kinetic model is proposed for gaseos phase flow between molten metal and foam material. The computations were performed for a wide range of Reynolds numbers. The results of the simulations are compared with the experiemental data obtained in this study.

Meshless local Petrov–Galerkin method-higher Reynolds numbers fluid flow applications

2012 ◽  
Vol 36 (11) ◽  
pp. 1671-1685 ◽  
Author(s):  
Mohammad Najafi ◽  
Ali Arefmanesh ◽  
Vali Enjilela
Keyword(s):  
Fluid Flow ◽  
Galerkin Method ◽  
Reynolds Numbers
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