Optimization of the extraction of galactoglucomannans from Pinus halepensis

AbstractThe effectiveness of pressurized hot-water extraction conditions for obtaining galactoglucomannans (GGMs) from Pinus halepensis suitable for applications like coatings and films packaging was investigated. For this purpose, high molar masses with high yields are required, presenting a serious challenge for hot-water extraction processes. The extraction of GGMs was carried out in an accelerated solvent extractor (ASE) and the isolation was performed by precipitation in ethanol. Three temperatures in the range 160–180 °C and five extraction times 5–90 min were tested in order to optimize extraction parameters of GGMs, avoiding thermal and chemical degradation in hot-water. Total dissolved solids (TDS) were determined gravimetrically after freeze-drying and weight average molar masses (Mw) were determined by high-performance size exclusion chromatography (HPSEC). Total non-cellulosic carbohydrates were determined by gas chromatography (GC) after acid methanolysis. Free monomers were additionally analyzed by GC. Lignin in water extracts was measured by an ultraviolet (UV) method. Acetic acid was determined after alkaline hydrolysis of acetyl groups and analyzed by HPSEC. The main parameters influencing the extraction processes of the GGMs, namely, extraction time and temperature were studied. Optimal extraction parameters of GGMs were identified at 170 °C and 20 min extraction time, with average Mw of extracted fraction of 7 kDa leading to a GGM yield of approximately 56 ${\text{mgg}}_{\text{o}.\text{d}.\text{m}}^{-1}$, corresponding to 6% on dry wood basis.

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Effect of Ultrasonic Wave on Lentinan Extraction

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.291-294.1339 ◽

2011 ◽

Vol 291-294 ◽

pp. 1339-1343

Author(s):

Wen Bo Zhang ◽

Hong Rui Li ◽

Jun Tao ◽

Bing Bing Dong

Keyword(s):

Extraction Efficiency ◽

Hot Water ◽

Water Extraction ◽

Extraction Technique ◽

Orthogonal Experimental Design ◽

Extraction Temperature ◽

Lentinus Edodes ◽

Extraction Condition ◽

Hot Water Extraction ◽

Extraction Parameters

The research in this paper optimized the extraction technique of lentinan with ultrasonic assistant method on the basis of hot water extraction technique, and investigated the promoting function of ultrasound to polysaccharides extraction. Extraction condition was selected by means of orthogonal experimental design, four factors and three levels L9(34), after key elements were respectively chosen through single factor experiments. Comparison between optimal extraction parameters of two method, hot water extraction technique and ultrasonic assistant extraction technique, showed decreased extraction temperature and significantly shortened extraction time, which existed in the second means, improved the extraction efficiency. Lentinus edodes polysaccharide extracted with ultrasonic assistant technique, the extraction rate and polysaccharide content percentage increased 6.22% and 8.66% respectively, comparative to which extracted with hot water extraction technique.

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Characterisation of Norway spruce hemicelluloses extracted by pressurised hot-water extraction (ASE) in the presence of sodium bicarbonate

Holzforschung ◽

10.1515/hf.2011.015 ◽

2011 ◽

Vol 65 (1) ◽

pp. 35-42 ◽

Cited By ~ 30

Author(s):

Tao Song ◽

Andrey Pranovich ◽

Bjarne Holmbom

Keyword(s):

Sodium Bicarbonate ◽

Norway Spruce ◽

Ph Value ◽

Hot Water ◽

Water Extraction ◽

Extraction Time ◽

Plain Water ◽

Hot Water Extraction ◽

Ph Conditions ◽

Optimal Ph

Abstract Ground spruce sapwood was extracted with pressurised hot water with addition of different amounts of sodium bicarbonate (NaHCO3) to find optimal pH conditions for extraction of hemicelluloses, especially of galactoglucomannans (GGMs). The extractions were carried out in an accelerated solvent extractor (ASE) at 170°C. When extraction was carried out with 2.5 mM NaHCO3 during 60–100 min extraction time (end-pH 3.9), more than 60% of the total extracted substances were hemicellulose-derived carbohydrates. Under these conditions, approximately 80% of the extracted carbohydrates were from GGMs (13% b.o. wood). At higher concentrations of NaHCO3, the yield of carbohydrates, especially those derived from GGMs, decreased. Hydrolytic depolymerisation of hemicelluloses occurring during extraction with plain water was largely inhibited in the presence of 2.5–5 mM NaHCO3. The hydrolytic deacetylation of GGM was diminished at low NaHCO3 concentrations but increased dramatically at higher NaHCO3 concentrations. It can be concluded that 2.5–5 mM NaHCO3 giving an end-pH value of 4 is better for GGM isolation than plain water.

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Optimization of extraction parameters of Pleurotus eryngii polysaccharides and evaluation of the hypolipidemic effect

RSC Advances ◽

10.1039/c9ra10991a ◽

2020 ◽

Vol 10 (20) ◽

pp. 11918-11928

Author(s):

Yuanyuan Zhao ◽

Xuefeng Chen ◽

Yanni Zhao ◽

Wei Jia ◽

Xiangna Chang ◽

...

Keyword(s):

Fruiting Body ◽

Pleurotus Eryngii ◽

Hot Water ◽

Water Extraction ◽

Hypolipidemic Effect ◽

Hot Water Extraction ◽

Extraction Parameters

The hot water extraction of polysaccharides from the fruiting body of Pleurotus eryngii was studied.

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Selective Extraction of Cannabinoid Compounds from Cannabis Seed Using Pressurized Hot Water Extraction

Molecules ◽

10.3390/molecules25061335 ◽

2020 ◽

Vol 25 (6) ◽

pp. 1335 ◽

Cited By ~ 1

Author(s):

Yannick Nuapia ◽

Hlanganani Tutu ◽

Luke Chimuka ◽

Ewa Cukrowska

Keyword(s):

Cannabis Sativa ◽

Polar Compounds ◽

Hot Water ◽

Water Extraction ◽

Extraction Time ◽

Extraction Temperature ◽

Plant Materials ◽

Hot Water Extraction ◽

Pressurized Hot Water Extraction ◽

Cannabinoid Compounds

Phytochemicals of Cannabis sativa mainly for the use in the different industries are that of delta-9-tetrahydrocannabinol (THC) and cannabidiol (CBD). Pressurized hot water extraction (PHWE) is seen as an efficient, fast, green extraction technique for the removal of polar and semi-polar compounds from plant materials. The PHWE technique was applied to extract cannabinoid compounds from Cannabis sativa seed. Response surface methodology was used to investigate the influence of extraction time (5–60 min), extraction temperature (50–200 °C) and collector vessel temperature (25–200 °C) on the recovery of delta-9-tetrahydrocannabinol (THC), cannabinol (CBN), cannabidiol (CBD), cannabichromene (CBG) and cannabigerol (CBC) from Cannabis sativa seed by PHWE. The identification and semi quantification of cannabinoid compounds were determined using GCXGC-TOFMS. The results obtained from different extractions show that the amount of THC and CBN was drastically decreasing in the liquid extract when the temperature rose from 140 to 160 °C in the extraction cell and the collector′s vessel. The optimal conditions to extract more CBD, CBC, and CBG than THC and CBN were set at 150 °C, 160 °C and 45 min as extraction temperature, the temperature at collector vessel, and the extraction time, respectively. At this condition, the predicted and experimental ratio of THCt (THC + CBN)/CBDt (CBD + CBC+ CBG) was found to be 0.17 and 0.18, respectively. Therefore, PHWE can be seen as an alternative to the classic extraction approach as the efficiency is higher and it is environmentally friendly.

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Optimization of ultrasonic extraction of Lycium barbarum polysaccharides using response surface methodology

International Journal of Food Engineering ◽

10.1515/ijfe-2020-0153 ◽

2020 ◽

Vol 0 (0) ◽

Author(s):

Jitian Song ◽

Shi Dongqi ◽

Su Hang ◽

Feng Yongxia ◽

Tian Wei

Keyword(s):

High Efficiency ◽

Hot Water ◽

Ultrasonic Extraction ◽

Water Extraction ◽

Extraction Time ◽

Ultrasonic Power ◽

Extraction Temperature ◽

Liquid Ratio ◽

Lycium Barbarum ◽

Hot Water Extraction

AbstractUltrasonic extraction was a new development method to achieve high-efficiency extraction of Lycium barbarum polysaccharides instead of hot water extraction. In this paper, the single factor method combined with the box Behnken design of response surface method was used to study the influence of extraction time, extraction temperature, material liquid ratio and ultrasonic power on the extraction rate of L. barbarum polysaccharide. The results indicated that the best extraction rate of L. barbarum polysaccharide was 12.54 ± 0.12% under the conditions of 80 min for extraction time, 73 °C for extraction temperature, 1 g:38 mL for material to liquid ratio, and 185 W for ultrasonic power. Under the same operating conditions, the yield of L. barbarum polysaccharide using ultrasonic extraction was 83.3%, which was higher than that hot water extraction. Moreover, the extraction time of ultrasonic extraction was only 47% of that using hot water extraction. This suggested that there was great potentials of using ultrasonic extraction in the realization of high-efficiency extraction of L. barbarum polysaccharide. The results of this study could also provide a theoretical basis for the coupling of ultrasonic extraction and ultrasonic concentration process to develop the integrated equipment of both ultrasonic extraction and ultrasonic concentration.

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