Anti-atherosclerotic activity of aqueous extract of Ipomoea batatas (L.) leaves in high-fat diet-induced atherosclerosis model rats

Abstract Objectives Cardiovascular diseases, especially atherosclerosis, are the leading cause of human mortality in Indonesia. Ipomoea batatas (L.) is a food plant used in Indonesian traditional medicine to treat cardiovascular diseases and related conditions. We assessed the anti-atherosclerotic activity of the aqueous extract of I. batatas leaves in a rat model of high-fat diet-induced atherosclerosis and its mechanism. Methods The presence of amino acid content in the I. batatas L. purple variant was determined by liquid chromatography high-resolution mass spectrometry (LC-HRMS). Thirty male Wistar rats were divided into five groups (n=6/group), i.e., standard diet group (SD), high-fat diet group (HF), and HF plus I. batatas L. extracts orally (625; 1,250; or 2,500 mg/kg) groups. The numbers of macrophages and aortic wall thickness were analyzed histologically. Immunohistochemical analyses were performed to assess foam cells-oxidized low-density lipoprotein (oxLDL), endothelial nitric oxide synthase (eNOS), and vascular endothelial growth factor (VEGF) expression in the aorta. Results LC-HRMS analysis showed nine amino acid content were identified from I. batatas L. In vivo study revealed that oral administration of I. batatas L. leaf extract alleviated foam cells-oxLDL formation and aortic wall thickness caused by high-fat diet atherosclerosis rats. Further, I. batatas L. leaf extract promoted the number of macrophages and modulated VEGF and eNOS expression in the aorta. Conclusions I. batatas L. leaf extract shows a positive anti-atherosclerosis effect. Furthermore, the mechanism may promote the macrophages, eNOS, VEGF expressions, and inhibition of foam cells-oxLDL formation and aortic wall thickness with the best dosage at 2,500 mg/kg. This could represent a novel approach to prevent cardiovascular diseases.

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Porphyromonas gingivalis vesicles reduce MDA-LDL levels and aortic wall thickness in high fat diet induced atherosclerosis rats

Artery Research ◽

10.1016/j.artres.2018.05.008 ◽

2018 ◽

Vol 23 (C) ◽

pp. 20 ◽

Cited By ~ 1

Author(s):

Aditya Indra Mahendra ◽

Jonny Karunia Fajar ◽

Harapan Harapan ◽

Teuku Heriansyah ◽

Sumarno Reto Prawiro ◽

...

Keyword(s):

Porphyromonas Gingivalis ◽

Wall Thickness ◽

High Fat Diet ◽

Aortic Wall ◽

High Fat ◽

Aortic Wall Thickness

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Deranged aortic intima-media thickness, plasma triglycerides and granulopoiesis in Sl/Sldmice

Mediators of Inflammation ◽

10.1080/09629350400008794 ◽

2004 ◽

Vol 13 (5-6) ◽

pp. 335-341 ◽

Cited By ~ 6

Author(s):

Kottarappat N. Dileepan ◽

Thomas P. Johnston ◽

Yuai Li ◽

Ossama Tawfik ◽

Daniel J. Stechschulte

Keyword(s):

Wall Thickness ◽

Peripheral Blood ◽

High Fat Diet ◽

Aortic Wall ◽

Plasma Cholesterol ◽

High Fat ◽

Dietary Regimen ◽

Plasma Triglycerides ◽

Aortic Wall Thickness ◽

The Impact

STUDIES were carried out to evaluate the impact of a high-fat dietary regimen on aortic wall thickness, peripheral blood leukocyte profile, and plasma cholesterol and triglyceride levels in the mast cell-deficient Sl/Sldmouse. The results demonstrated that the mean aortic wall thickness of Sl/Sldmice was significantly higher than their normal littermates, and were increased in both genotypes after a 17-day high-fat regimen. In comparison with normal littermates, Sl/Sldgenotypes had elevated levels of plasma triglycerides with normal levels of plasma cholesterol, and the high-fat diet markedly lowered the triglyceride levels. Total peripheral blood leukocytes, the monocyte and granulocyte counts, and hemoglobin levels were significantly lower in Sl/Sldmice, although the number of lymphocytes, eosinophils and basophils were the same in both genotypes. Interestingly, the high-fat diet regimen elevated leukocyte counts and the number of monocytes and granulocytes in Sl/Sldmice.

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Maternal undernutrition reduces aortic wall thickness and elastin content in offspring rats without altering endothelial function

Clinical Science ◽

10.1042/cs20060036 ◽

2006 ◽

Vol 111 (4) ◽

pp. 281-287 ◽

Cited By ~ 28

Author(s):

Michael R. Skilton ◽

Alison K. Gosby ◽

Ben J. Wu ◽

Lisa M. L. Ho ◽

Roland Stocker ◽

...

Keyword(s):

Wall Thickness ◽

Aortic Wall ◽

Infant Nutrition ◽

Protein Diet ◽

Low Protein Diet ◽

Organ Bath ◽

High Fat ◽

Maternal Undernutrition ◽

Low Protein ◽

Aortic Wall Thickness

Epidemiological studies suggest a link between fetal/early infant nutrition and adult coronary artery disease. In the present study, we examined the effects of altering nutrition during gestation, lactation and juvenile life on aortic structure and function in rats. Wistar rat dams were fed either a control or low-protein diet throughout pregnancy, or a low-protein diet for the final 7 days of gestation only. At 21 days post-partum, male pups were weaned on to a control, low-protein or high-fat diet. At 12 weeks, the offspring rats were killed. In 46 rats, aortic sections were mounted and stained to assess media thickness and elastin content. In a further 38 rats, aortic rings were suspended in an organ bath and vascular reactivity was tested with dose–response curves to the endothelium-dependent dilator acetylcholine and the endothelium-independent dilator sodium nitroprusside. Rats exposed to maternal protein restriction while in utero had a significantly decreased aortic wall thickness compared with control rats (P=0.005). Total elastin content of the aorta was also decreased by both maternal low-protein (P=0.02) and early postnatal low-protein (P=0.01) diets. Neither maternal nor postnatal low-protein or high-fat diets, however, resulted in any significant changes in arterial dilator function. In conclusion, fetal undernutrition in rats, induced via a maternal low-protein diet, causes a decrease in aortic wall thickness and elastin content without altering aortic dilator function. These changes in vascular structure may amplify aging-related changes to the vasculature and contribute to the pathophysiology of the putative link between impaired fetal growth and adult cardiovascular disease.

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Efficacy of purple sweet potato (Ipomoea batatas) extract to prevent the aortic wall thickness of white male rats atherosclerosis model

Medicinal Plants - International Journal of Phytomedicines and Related Industries ◽

10.5958/0975-6892.2019.00002.9 ◽

2019 ◽

Vol 11 (1) ◽

pp. 55

Author(s):

Meddy Setiawan ◽

Suhartono Taat Putra ◽

Djanggan Sargowo

Keyword(s):

Sweet Potato ◽

Wall Thickness ◽

Ipomoea Batatas ◽

Aortic Wall ◽

White Male ◽

Male Rats ◽

Aortic Wall Thickness ◽

Purple Sweet Potato

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GW29-e1172 TNF-α, Foam Cells and Abdominal Aortic Wall Thickness in Rats with Subchronical Inhalation Exposure of Transfluthrin

Journal of the American College of Cardiology ◽

10.1016/j.jacc.2018.08.132 ◽

2018 ◽

Vol 72 (16) ◽

pp. C35

Author(s):

Asri Prameswari ◽

Djoko Wahono Soeatmadji

Keyword(s):

Wall Thickness ◽

Aortic Wall ◽

Inhalation Exposure ◽

Foam Cells ◽

Abdominal Aortic ◽

Tnf Α ◽

Aortic Wall Thickness

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TNF-α, foam cells and abdominal aortic wall thickness in rats with subchronical inhalation exposure of transfluthrin

Heart Lung and Circulation ◽

10.1016/j.hlc.2015.06.189 ◽

2015 ◽

Vol 24 ◽

pp. S192 ◽

Cited By ~ 1

Author(s):

A. Prameswari ◽

D. Soeatmadji

Keyword(s):

Wall Thickness ◽

Aortic Wall ◽

Inhalation Exposure ◽

Foam Cells ◽

Abdominal Aortic ◽

Tnf Α ◽

Aortic Wall Thickness

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Bisflavonoids fraction from Araucaria bidwilli Hook., reverses hyperlipidemia induced atherosclerosis in high-fat diet induced hyperlipidemia

Future Journal of Pharmaceutical Sciences ◽

10.1186/s43094-020-00109-y ◽

2020 ◽

Vol 6 (1) ◽

Author(s):

Rahimullah Siddiqui ◽

Haja Nazeer Ahamed ◽

Ismail Yusuff

Keyword(s):

High Fat Diet ◽

Leaf Extract ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Heart Tissue ◽

High Fat ◽

Anti Oxidant ◽

Aortic Wall Thickness

Abstract Background Hyperlipidemia is a major cause for atherosclerosis which is a frontline cause for mortality in the world. Bisflavonoids are dimeric flavonoids abundant in few medicinal herbs with various pharmacological effects. However, in vivo anti-hyperlipidemic role of bisflavonoids (BFR) is limited. The present investigation is aimed to study BFR from the leaf extract of Araucaria bidwillii Hook. in rat model of hyperlipidemia. Results Administration of HFD was significantly (p < 0.0001) shown to increase total cholesterol (TC), low-density lipoprotein (LDL), and triglycerides (TG) associated with decrease in HDL. BFR at two doses significantly decreased TC, LDL, and TG in HFD-fed rats. In addition, BFR significantly (p < 0.0001) decreased the MDA and significantly (p < 0.0001) increased the impaired anti-oxidant enzyme SOD and CAT in heart tissue induced by HFD. Further, 28 days administration of BFR significantly (p < 0.001) decreased HFD-induced aortic wall thickness. Conclusion It can be concluded that bisflavonoids from A. bidwillii Hook. leaf extract administered to high fat-fed rats showed beneficial anti-hyperlipidemic effect by reducing lipid profiles and protecting the heart tissue from oxidative stress.

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