Bestimmung der Glukosekonzentration im Venenblut mit verschiedenen Glukometern zur Blutzuckerselbstkontrolle im Vergleich zur Glukoseoxidase (GOD)-Methode am ESAT 6660-2/ECA 2000. Determination of Glucose Levels in Venous Blood by the Use of Various Glucometers for Glucose Self Monitoring Compared to the Glucose-Oxidase-Method on the ESAT 6660-2/ECA 2000

2000 ◽  
Vol 24 (9) ◽  
pp. 397-406
Author(s):  
H.-G. Lestin ◽  
M. Augsten ◽  
F. Lestin ◽  
M. Hergert ◽  
G. Winkler ◽  
...  
Keyword(s):  
Glucose Oxidase ◽  
Venous Blood ◽  
Self Monitoring ◽  
Glucose Levels

A New and Rapid Method for the Determination of Glucose by Measurement of Rate of Oxygen Consumption

1968 ◽  
Vol 14 (2) ◽  
pp. 116-131 ◽  
Author(s):  
Arnold Henry Kadish ◽  
Robert L Litle ◽  
James C Sternberg
Keyword(s):  
Hydrogen Peroxide ◽  
Oxygen Consumption ◽  
Glucose Oxidase ◽  
Oxygen Sensor ◽  
Direct Measure ◽  
Peroxide Formation ◽  
Glucose Levels ◽  
Rate Of Oxygen Consumption ◽  
Hydrogen Peroxide Formation

Abstract Glucose levels in serum, plasma, and urine are determined rapidly and conveniently by a new glucose oxidase method employing a polarographic oxygen sensor with a circuit modified to record the rate of oxygen consumption. The maximum apparent rate of oxygen consumption relative to the rate obtained with a glucose standard provides a direct measure of the glucose level in the sample; results are obtainable within 20 sec. after sample (100 µl.) addition and within 3 min. after a blood sample is withdrawn from a patient. Interferences associated with prior colorimetric glucose oxidase methods are avoided by measuring oxygen consumption instead of hydrogen peroxide formation. The method is described and results are presented showing a standard deviation of less than 1.5% on replicate determinations and a bias of 1% with respect to data obtained on the same samples by the automated ferricyanide method.

The Rapid Prehospital Estimation of Blood Glucose Using Chemstrip bG

1989 ◽  
Vol 4 (2) ◽  
pp. 109-113 ◽  
Author(s):  
Paul T. Hogya ◽  
Donald M. Yealy ◽  
Paul M. Paris ◽  
Ronald D. Stewart
Keyword(s):  
Sensitivity And Specificity ◽  
Venous Blood ◽  
Rapid Identification ◽  
Serum Glucose ◽  
Altered Level ◽  
Glucose Levels ◽  
Level Of Consciousness ◽  
Reagent Strips ◽  
Blood Specimens

AbstractThe accuracy of the glucose reagent strip, Chemstrip bG, for the determination of the presence of hypoglycemia, euglycemia, or hyperglycemia was assessed in the prehospital setting when used by paramedics treating patients with an altered level of consciousness. Venous blood specimens were obtained from each of sixty-two patients. Serum glucose levels of the sample were determined by paramedics in the field using the Chemstrip bG and within three hours after sample acquisition by standard laboratory techniques. The sensitivity and specificity of detecting true hypoglycemia (serum glucose<61 mg/dl) using the field reagent strips were 100% and 83.3% respectively. The corresponding 95% lower limits of confidence (LLC) were 81.3% and 83 .3%. The sensitivity and specificity of field detection of the absence of hypoglycemia (serum glucose ≥ 61 mg/dl) were 86.6 % (LLC=80.9%) and 100% (LLC=81.3%) respectively. The accuracy of the reagent strips is specific but not sensitive for the determination of the presence of hyperglycemia (serum glucose > 179 mg/dl). Thus, the Chemstrip bG is an accurate means for the rapid identification of the presence or absence of hypoglycemia in the field setting by paramedics.

Design and Fabrication of Glucose Biosensors Based on Immobilization of Glucose Oxidase on Titanium Oxide Nanotube Arrays

2021 ◽  
Vol 21 (9) ◽  
pp. 4605-4614
Author(s):  
Andrew Du ◽  
Eva Alvarez De Eulate ◽  
Alex Hariz
Keyword(s):  
Glucose Oxidase ◽  
Titanium Substrate ◽  
Ammonium Fluoride ◽  
Reduction Reaction ◽  
Nanotube Arrays ◽  
Nanotube Array ◽  
Glucose Levels ◽  
Enhanced Sensitivity ◽  
Anodization Process

An electrochemical biosensor for the detection of glucose is realized by immobilizing glucose oxidase (GOx) enzyme onto titanium dioxide nanotube arrays by a coupling encapsulation process. We present details of a robust fabrication technique that results in a durable and reproducible sensor characteristics. The TiO2 nanotube arrays are grown directly on a titanium substrate by a potentiostatic anodization process in a water and ethylene-glycol mixture solution, which contains ammonium fluoride. An electropolymerization process was also performed to enhance interfacial adhesion between GOx and TiO2 nanotubes. Detection of glucose concentrations was achieved with a linear response in the range of 0.01 to 0.2 mM. Investigation of enhanced sensitivity by increasing the count, the length, and the cross-section of the nanotubes was also carried out. Surface morphologies of Ti substrate were examined by scanning electron microscopy to optimize the anodization process and thus the TiO2/Ti nanotube dimensions. We utilized a time-based amperometric response for the quantitative determination of hydrogen peroxide concentration through electro-reduction reaction with a bare TiO2/Ti nanotube-array electrodes, thus providing a reference for the determination of glucose levels with a GOx-coated TiO2/Ti nanotube array electrodes. Detection levels down to 5.2 μM were recorded.

scholarly journals Determination of glucose levels using dried filter paper blood spots: new perspective in home monitoring

1996 ◽  
Vol 114 (1) ◽  
pp. 1100-1103 ◽  
Author(s):  
Laura Sterian Ward ◽  
Renata Britto Novis ◽  
Veridiana Toledo Nascimento ◽  
Miriam Siesler Nóbrega ◽  
Mário José Abdalla Saad
Keyword(s):  
Diabetes Mellitus ◽  
Filter Paper ◽  
Venous Blood ◽  
Home Monitoring ◽  
Glucose Levels ◽  
Significant Difference ◽  
Cheap Alternative ◽  
Control Of Diabetes ◽  
New Perspective

We present a method for the determination of blood glucose using dried filter paper blood spots.To validate this method, we compared our results using filter paper and simultaneously collected venous blood. We demonstrated that there is a linear relationship between the filter paper glucose levels and those determined in whole blood (r=0.98). There was no significant difference between the results of the two methods (p>0.05).This method is a cheap alternative which may improve the control of diabetes mellitus, and may also be very useful in the diagnosis of postprandial hypoglycemia and other special situations.

The Effects of Self-monitoring and Physical Appearance on the Determination of General Attraction

1999 ◽  
Author(s):  
Sebastian Laube ◽  
Rachel Silger ◽  
Christy Williams ◽  
Julie Schuldt
Keyword(s):  
Physical Appearance ◽  
Self Monitoring

Assessment of the incorporation of revascularized fibula grafts used for mandibular reconstruction with F-18-PET

2001 ◽  
Vol 40 (02) ◽  
pp. 51-58 ◽  
Author(s):  
H. Schliephake ◽  
van den Hoff ◽  
W. H. Knapp ◽  
G. Berding
Keyword(s):  
Blood Flow ◽  
Regional Blood Flow ◽  
Venous Blood ◽  
Mandibular Reconstruction ◽  
Reference Region ◽  
Osteoblastic Activity ◽  
Non Union ◽  
Range Of Values ◽  
Measured Input

Summary Aim: Determination of the range of regional blood flow and fluoride influx during normal incorporation of revascularized fibula grafts used for mandibular reconstruction. Evaluation, if healing complications are preceded by typical deviations of these parameters from the normal range. Assessment of the potential influence of using “scaled population-derived” instead of “individually measured” input functions in quantitative analysis. Methods: Dynamic F-l 8-PET images and arterialized venous blood samples were obtained in 11 patients early and late after surgery. Based on kinetic modeling regional blood flow (K1) and fluoride influx (Kmlf) were determined. Results: In uncomplicated cases, early postoperative graft K1 - but not Kmlf -exceeded that of vertebrae as reference region. Kmn values obtained in graft necrosis (n = 2) were below the ranges of values observed in uncomplicated healing (0.01 13-0.0745 ml/min/ml) as well as that of the reference region (0.0154-0.0748). Knf values in mobile non-union were in the lower range - and those in rigid non-union in the upper range of values obtained in stable union (0.021 1-0.0694). If scaled population-derived instead of measured input functions were used for quantification, mean deviations of 23 ± 17% in K1 and 12 ± 16% in Kmlf were observed. Conclusions: Normal healing of predominantly cortical bone transplants is characterized by relatively low osteoblastic activity together with increased perfusion. It may be anticipated that transplant necrosis can be identified by showing markedly reduced F− influx. In case that measured input functions are not available, quantification with scaled population-derived input functions is appropriate if expected differences in quantitative parameters exceed 70%.

THE APPLICATION OF GAS-LIQUID RADIOCHROMATOGRAPHY TO THE QUANTITATIVE DETERMINATION OF STEROIDS IN THE PERIPHERAL VENOUS BLOOD OF NON-PREGNANT WOMEN

1965 ◽  
Vol 49 (3_Suppl) ◽  
pp. S204
Author(s):  
Ian F. Sommerville ◽  
Brian M. Sheerin ◽  
William P. Collins ◽  
Heather Wyman
Keyword(s):  
Quantitative Determination ◽  
Pregnant Women ◽  
Venous Blood ◽  
Peripheral Venous Blood
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