scholarly journals Determination of indirect reference intervals of vitamin B12 for outpatients admitted to primary healthcare centers

2020 ◽  
Vol 44 (1) ◽  
pp. 11-16
Author(s):  
Esin Avci ◽  
Diler Aslan
Keyword(s):  
Vitamin B12 ◽  
Primary Healthcare ◽  
Clinical Chemistry ◽  
Age Groups ◽  
Vitamin B12 Deficiency ◽  
Reference Intervals ◽  
Microcytic Anemia ◽  
Reference Limits ◽  
B12 Deficiency

Abstract Background Vitamin B12 deficiency is still an important nutritional problem worldwide. There is no consensus on the reference intervals (RIs) for vitamin B12. In order to establish vitamin B12 RIs, we used the database obtained from a primary healthcare organization and estimated region-specific intervals. Methods Vitamin B12 results were collected from our laboratory information system (LIS) between January 2014 and July 2014. We excluded the results of the individuals who had megaloblastic and microcytic anemia. After outlier detection and exclusion, we estimated the RIs of the “1–12, 13–18, 19–64 and 65 +” age groups according to the International Federation of Clinical Chemistry (IFCC) method. Statistical differences were examined between groups. Results The reference limits and 90% confidence intervals (CIs) (n = 14,748, 3790 male, age: 1–96; 10,958 female, age: 1–115) were estimated with 90% CIs as 106 (104–107)–393 (391–395) pmol/L. We also estimated the reference limits of the “1–12, 13–18, 19–64 and 65 +” age groups to be 127–459, 108–398, 106–384 and 104–426 pmol/L, respectively. There was no difference between genders in all age groups. Conclusions Both the lower and upper limits of the estimated RIs were found to be lower than the reference limits recommended by the manufacturer. Our findings revealed that the RIs of vitamin B12 should be given for the “1–12, 13–18, 19–64 and 65 +” age groups separately. The big data obtained from laboratories should be used for the determination of its population health status. As our population is composed of outpatients, the estimated vitamin B12 RIs may establish the basic information for the examination of our population for vitamin B12 deficiency.

Vitamin B12 Deficiency an Unusual Cause of Fever: A Case Report

2019 ◽  
Vol 4 (02) ◽  
Author(s):  
Shyama . ◽  
P. Kumar ◽  
Surabhi .
Keyword(s):  
Bone Marrow ◽  
Vitamin B12 ◽  
Unusual Case ◽  
Vitamin B12 Deficiency ◽  
Bone Marrow Aspiration ◽  
Microcytic Anemia ◽  
Peripheral Smear ◽  
Erythroid Hyperplasia ◽  
B12 Deficiency ◽  
Vitamin B12 Supplementation

Introduction: An unusual case of a 19 year old female, presenting with fever, pallor and hepatosplenomegaly for one month. She had microcytic anemia on peripheral smear examination but her bone marrow aspiration & biopsy revealed a hypercelluar marrow with megaloblastic erythroid hyperplasia. Resolution of fever within 48 hours of Vitamin B12 supplementation, initiated in view of the megaloblastic bone marrow picture & low serumVitamin B12 level, suggests a causal association. Conclusion: Vitamin B12 deficiency seems to be an unusual cause of PUO (Pyrexia of unkown origin) which should be ruled out in every case of PUO.

scholarly journals Microcytic Anemia Hiding Vitamin B12 Deficiency Anemia

Cureus ◽  
2021 ◽  
Author(s):  
Fadi Busaleh ◽  
Omkolthoom A Alasmakh ◽  
Fatimah Almohammedsaleh ◽  
Maram F Almutairi ◽  
Juwdaa S Al Najjar ◽  
...  
Keyword(s):  
Vitamin B12 ◽  
Vitamin B12 Deficiency ◽  
Microcytic Anemia ◽  
Deficiency Anemia ◽  
B12 Deficiency

scholarly journals Dietary Intake and Status of Vitamin B12 in Slovenian Population

Nutrients ◽  
2022 ◽  
Vol 14 (2) ◽  
pp. 334
Author(s):  
Živa Lavriša ◽  
Hristo Hristov ◽  
Maša Hribar ◽  
Katja Žmitek ◽  
Anita Kušar ◽  
...  
Keyword(s):  
Vitamin B12 ◽  
Age Groups ◽  
Serum Vitamin ◽  
Vitamin B12 Deficiency ◽  
High Serum ◽  
Health Concern ◽  
Dietary Vitamin ◽  
Serum Homocysteine ◽  
Daily Vitamin ◽  
B12 Deficiency

Vitamin B12 deficiency poses a health concern, especially in vulnerable populations. Dietary vitamin B12 intake was obtained by two 24 h dietary recalls and food propensity questionnaires in a representative Slovenian cross-sectional food consumption survey, SI.Menu (n = 1248 subjects; 10–74 years). For a subgroup of 280 participants, data on serum vitamin B12 were available through the Nutrihealth study. The estimated usual population-weighted mean daily vitamin B12 intakes were 6.2 µg (adults), 5.4. µg (adolescents), and 5.0 µg (elderly). Lower intakes were observed in females. Inadequate daily vitamin B12 intake (<4 µg) was detected in 37.3% of adolescents, 31.7% of adults, and 58.3% elderlies. The significant predictors for inadequate daily vitamin B12 intake were physical activity score in all age groups, sex in adolescents and adults, financial status and smoking in elderly, and employment in adults. Meat (products), followed by milk (products), made the highest vitamin B12 contribution in all age groups. In adolescents, another important vitamin B12 contributor was cereals. The mean population-weighted serum vitamin B12 levels were 322.1 pmol/L (adults) and 287.3 pmol/L (elderly). Low serum vitamin B12 concentration (<148 nmol/L) and high serum homocysteine (>15 µmol/L) were used as criteria for vitamin B12 deficiency. The highest deficiency prevalence was found in elderlies (7.0%), particularly in males (7.9%). Factors associated with high serum homocysteine were also investigated. In conclusion, although vitamin B12 status was generally not critical, additional attention should be focused particularly to the elderly.

Recommendation for the review of biological reference intervals in medical laboratories

2016 ◽  
Vol 54 (12) ◽  
Author(s):  
Joseph Henny ◽  
Anne Vassault ◽  
Guilaine Boursier ◽  
Ines Vukasovic ◽  
Pika Mesko Brguljan ◽  
...  
Keyword(s):  
Expert Panel ◽  
Clinical Chemistry ◽  
Simple Procedure ◽  
Reference Population ◽  
Reference Intervals ◽  
Medical Laboratories ◽  
Reference Limits ◽  
Original Procedure ◽  
Selection Of

AbstractThis document is based on the original recommendation of the Expert Panel on the Theory of Reference Values of the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC), updated guidelines were recently published under the auspices of the IFCC and the Clinical and Laboratory Standards Institute (CLSI). This document summarizes proposals for recommendations on: (i) The terminology, which is often confusing, noticeably concerning the terms of reference limits and decision limits. (ii) The method for the determination of reference limits according to the original procedure and the conditions, which should be used. (iii) A simple procedure allowing the medical laboratories to fulfill the requirements of the regulation and standards. The updated document proposes to verify that published reference limits are applicable to the laboratory involved. Finally, the strengths and limits of the revised recommendations (especially the selection of the reference population, the maintenance of the analytical quality, the choice of the statistical method used…) will be briefly discussed.

scholarly journals Determination of Vitamin B12 Deficiency Status in Vegan and Non-Vegan Thais by Assessment of Homocysteine Level

2021 ◽  
Vol 17 (01) ◽  
pp. 15-19
Author(s):  
Sant Chaiyodsilp ◽  
Sirada Phuriwattanapong ◽  
Weerapong Chaiyaphak ◽  
Somwong Chaiyodsilp ◽  
Phasuk Pruisart
Keyword(s):  
Vitamin B12 ◽  
Homocysteine Level ◽  
Vitamin B12 Deficiency ◽  
B12 Deficiency

Reference intervals developed from data for hospitalized patients: computerized method based on combination of laboratory and diagnostic data

1994 ◽  
Vol 40 (12) ◽  
pp. 2209-2215 ◽  
Author(s):  
T Kouri ◽  
V Kairisto ◽  
A Virtanen ◽  
E Uusipaikka ◽  
A Rajamäki ◽  
...  
Keyword(s):  
Mean Corpuscular Volume ◽  
Age Groups ◽  
Hemoglobin Concentration ◽  
Reference Intervals ◽  
Hospitalized Patients ◽  
Healthy Individuals ◽  
Reference Limits ◽  
Health Related ◽  
Final Group

Abstract We utilized the databases of a hospital information system to select for determination of reference values various individual hospitalized patients on the basis of their diagnoses at discharge. The nonparametric 2.5-97.5% "health-related" reference intervals were calculated for hemoglobin concentration, mean corpuscular volume (MCV), and erythrocyte count for both sexes. After excluding patients with diseases possibly affecting erythrocyte variables, we obtained a final group of 1786 women and 1450 men, ages 20-65 years, who were studied in age groups of 20-30, 30-45, 45-55, and 55-65 years. The upper reference limits of the MCV results obtained from hospitalized patients were higher than those produced conventionally from healthy individuals, as would be intuitively suggested by clinical experience. This method, based on selection by diagnosis, could be applicable to various analytes measured in hospital laboratories, provided sufficient data are available as databases.

scholarly journals Proportion and Associated Factors of Vitamin B12 Deficiency - A Retrospective Laboratory-Based Study

2019 ◽  
Vol 7 (3) ◽  
pp. 670-677
Author(s):  
K Sowndarya ◽  
Sridevi Hanaganahalli Basavaiah ◽  
Ramya Shenoy ◽  
Anupama Hegde
Keyword(s):  
Vitamin B12 ◽  
Associated Factors ◽  
Laboratory Data ◽  
Age Groups ◽  
Vitamin B12 Deficiency ◽  
Gastric Surgery ◽  
Folic Acid Deficiency ◽  
Age Group ◽  
Rbc Indices ◽  
B12 Deficiency

Vitamin B12 is a vital micronutrient involved in the hemopoetic, neurologic and cardio-metabolic function. Various factors are responsible for B12 deficiency, which can lead to anemia and irreversible neurological manifestations. The proportion of deficiency is not well known and hence studied in the hospital setup. The associated factors of B12 deficiency were also deduced in different age groups. Laboratory data of the past two years on vitamin B12 (B12), folate and red blood cell (RBC) indices were compiled. Based on B12 values, subjects were grouped as deficient, subclinical deficiency and normal. RBC indices especially, Mean corpuscular volume (MCV) was compared among all three groups. The deficient group was further categorized based on age to know the associated factors as per the diagnosis or medical impression recorded. The frequency of B12 and folic acid deficiency was found to be 28% and 18.8% respectively. The deficiency was more frequently detected in the younger age group (18-30years) with inadequate nutrition, pregnancy and vegetarian diet being the most frequently associated factors. Hyperthyroidism was an add-on in the mid-age group. In subjects aged 51-70 years, disorders of intestine, gastric surgery, cancer and pernicious anemia were the attributing factors. MCV > 100fL was found only in 4.8% of the B12 deficient population. Only 28% of B12 deficiency could be conclusively detected. Pregnancy and nutritional status were the most commonly associated factors of B12 deficiency. The use of MCV for the detection of B12 deficiency did not seem reliable.

scholarly journals Prevalence of iron, folate, and vitamin B12 deficiencies in 20 to 49 years old women: Ensanut 2012

2015 ◽  
Vol 57 (5) ◽  
pp. 385 ◽  
Author(s):  
Teresa Shamah-Levy ◽  
Salvador Villalpando ◽  
Fabiola Mejía-Rodríguez ◽  
Lucía Cuevas-Nasu ◽  
Elsa Berenice Gaona-Pineda ◽  
...  
Keyword(s):  
Iron Deficiency ◽  
Vitamin B12 ◽  
Urban Areas ◽  
Age Groups ◽  
Vitamin B12 Deficiency ◽  
Reproductive Age ◽  
Serum Folate ◽  
Women Of Reproductive Age ◽  
Nutrition Survey ◽  
B12 Deficiency

 Objective. To describe the prevalence of iron, folate, and B12 deficiencies in Mexican women of reproductive age from the National Health and Nutrition Survey (Ensanut) 2012.Materials and methods. Data came from a  ationalprobabilistic survey, representative from rural and urban areas,and different age groups. Blood samples were obtained from 4 263, 20 to 49 years old women for serum ferritin, vitamin B12 and serum folate oncentrations. The prevalence of deficiencies, was assessed using adjusted logistic regression models. Results. The deficiency of folate was 1.9% (95%CI1.3-2.8), B12 deficiency was 8.5% (95%CI 6.7-10.1) and iron deficiency was 29.4% (95%CI 26.5-32.2). No differences were found when compared with 2006, 24.8% (95%CI 22.3-27.2).Conclusions. The vitamin B12 deficiency is still a problem for women of reproductive age and their offspring in Mexico,while folate deficiency disappeared as a problem. Iron deficiency needs prevention and fortification strategies. 

Sign in / Sign up

Export Citation Format

Share Document