scholarly journals Isolation of nucleic acids using silicon dioxide powder as a tool for environmental monitoring

2019 ◽  
Vol 191 (12) ◽  
Author(s):  
Jacek Urbaniak ◽  
Daniel Janowski ◽  
Brayan Jacewski
Keyword(s):  
Nucleic Acids ◽  
Silicon Dioxide ◽  
Environmental Monitoring ◽  
Cost Effective ◽  
Ease Of Use ◽  
Isolation And Purification ◽  
Long Run ◽  
Dioxide Powder ◽  
Commercial Kits ◽  
The Cost

Abstract Isolation and purification of nucleic acids are basic laboratory procedures used in molecular analysis supporting determination of organisms in environmental monitoring. However, many different methods of isolation are commonly used, often being designed for a particular type of DNA extraction. While researchers commonly decide on commercial isolation kits for their ease of use and efficiency, they require large amounts of studied tissue, and the cost of purchasing such kits over a long run can be high. To provide an alternative to using commercial kits, we have developed a simple, rapid, cost-effective, and reliable protocol for DNA isolation from cultured fungi on slants and from dried fungal samples using silica particles (silicon dioxide powder) in chaotropic conditions. With the presented method, it is possible to isolate good-quality DNA from fungi in less than 1.5 h, using easily accessible chemicals. Compared with other methods employing CTAB or commercial kits, it allows fast, easy, and cheap DNA purification from two main sources of fungi routinely used for research. In addition to the method protocol, we also provide advice for further optimization of the isolation process to account for specific conditions, making the procedure more useful.

scholarly journals Complex whey utilization: the propionic acid alternative

2017 ◽  
Vol 2 (1) ◽  
pp. 9-12 ◽  
Author(s):  
Aladár Vidra ◽  
András József Tóth ◽  
Áron Németh
Keyword(s):  
Lactic Acid ◽  
Propionic Acid ◽  
Cost Effective ◽  
Acid Fermentation ◽  
Isolation And Purification ◽  
Application Range ◽  
Lactic Acid Content ◽  
Lactose Utilization ◽  
Acid Producing Bacteria ◽  
The Cost

Abstract Whey is the complex waste of the dairy industry. Despite the fact, that it has numerous applications (like different form of food supplements), its major amount is still handled as waste. The carbohydrate, protein and lactic acid content, as well as the COD and BOD, are sufficiently high warranting disposal as waste resulting in high costs; however, their levels are insufficient for the cost-effective isolation and purification. Most of the numerous reports on whey utilisation focus on lactose utilization, while lactic acid removal is complex, but necessary, particularly in case of sour whey decontamination. According to our best knowledge among the microbial fermentation, the only lactic acid (as carbon source) utilization process is propionic acid fermentation. Propionic acid is an attractive product with a wide application range. In this study, two propionic acid producing microorganisms were investigated in terms of industrial applicability. The propionic acid producing bacteria are generally characterized by anaerobic metabolism (except the pathogenic P. acne); but, for application in a biorefinery, facultative anaerobe behavior is the most appropriate and cost-effective. In this study, the aero-tolerances of Propionibacterium freudenreichii subsp. shermanii and Propionibacterium acidipropionici were examined; their propionic acid-producing properties (yield, concentration, substrate preference, productivity) were compared.

scholarly journals A streamlined, cost-effective, and specific method to deplete transcripts for RNA-seq

2020 ◽  
Author(s):  
Amber Baldwin ◽  
Adam R Morris ◽  
Neelanjan Mukherjee
Keyword(s):  
Cost Effective ◽  
Circular Rnas ◽  
Specific Method ◽  
Rna Seq ◽  
Gold Standard Method ◽  
Wide Range ◽  
Rrna Depletion ◽  
Commercial Kits ◽  
The Cost

RNA-sequencing is a powerful and increasingly prevalent method to answer biological questions. Depletion of ribosomal RNA (rRNA), which accounts for 80% of total RNA, is an extremely important step to increase the power of RNA-seq. Selection for polyadenylated RNA is a commonly used approach that excludes rRNA, as well as, important non-polyadenylated RNAs, such as histones, circular RNAs, and many long noncoding RNAs. Commercial methods to deplete rRNA are cost-prohibitive and the gold standard method is no longer available as a standalone kit. Alternative non-commercial methods suffer from inconsistent depletion. Through careful characterization of all reaction parameters, we developed an optimized RNaseH-based depletion of human rRNA. Our method exhibited comparable or better rRNA depletion compared to commercial kits at a fraction of the cost and across a wide-range of input RNA amounts.

scholarly journals An assessment of hydrates inhibition in deepwater production systems using low-dosage hydrate inhibitor and monoethylene glycol

2019 ◽  
Vol 10 (3) ◽  
pp. 1169-1182 ◽  
Author(s):  
Ndubuisi U. Okereke ◽  
Pius E. Edet ◽  
Yahaya D. Baba ◽  
Nkemakolam C. Izuwa ◽  
Sunday Kanshio ◽  
...  
Keyword(s):  
Production Systems ◽  
Cost Effective ◽  
Integrated Production ◽  
Long Run ◽  
Formation Of Hydrates ◽  
The Cost ◽  
Monoethylene Glycol ◽  
Production Modelling ◽  
Low Dosage ◽  
Flow Stream

AbstractIn this study, a deepwater pipeline-riser system that experienced hydrates was modelled in MAXIMUS 6.20 (an integrated production modelling tool) to understand, predict and mitigate hydrates formation in typical deepwater system. Highlights of the results from this study suggest that the injection of low-dosage hydrate inhibitors (LDHIs) into the hydrate-forming structures within the multiphase flow stream disperses the hydrates particles in an irregular manner and subsequently decreases the nucleation rate of the hydrate and prevents the formation of hydrates. This study found that the cost of using monoethylene glycol was significantly higher than that of LDHI by over $500/day although low-dosage hydrate inhibitors have initial relatively high CAPEX. In the long run, its OPEX is relatively low, making it cost-effective for hydrate inhibition in deepwater scenarios.

scholarly journals Cost-Effective Laparoscopic Cholecystectomy

2009 ◽  
Vol 91 (8) ◽  
pp. 670-672 ◽  
Author(s):  
M Slater ◽  
MI Booth ◽  
TCB Dehn
Keyword(s):  
Laparoscopic Cholecystectomy ◽  
Cost Effective ◽  
Ease Of Use ◽  
Hospital Data ◽  
Grand Total ◽  
Additional Costs ◽  
Disposable Instruments ◽  
The Cost ◽  
Disposable Equipment ◽  
Upper Gastrointestinal

INTRODUCTION There is wide variation in costs, both theatre and ward, for the same operation performed in different hospitals. The aim of this study was to compare the true costs for a large number of consecutive laparoscopic cholecystectomy (LC) cases using re-usable equipment with those from an adjacent trust in which the policy was to use disposable LC equipment. PATIENTS AND METHODS Data were collected prospectively between January 2001 and December 2007 inclusive for all consecutive patients undergoing LC by two upper gastrointestinal (UGI) consultants at the Royal Berkshire Hospital. Data were collected for all the instruments used, in particular any additional disposable instruments used at surgeons' preference. Sterilisation costs were calculated for all re-usable instruments. Costs were also obtained from an adjacent NHS trust which adopted a policy of using disposable ports and clip applicators. Disposable equipment such as drapes, insufflation tubing, and camera sheath were not considered as additional costs, since they are common to both trusts and not available in a re-usable form. RESULTS Over 7 years, a total of 1803 LCs were performed consecutively by two UGI consultants at the Royal Berkshire Hospital. The grand total for 1803 LC cases for the re-usable group, including initial purchasing, was £89,844.41 (an average of £49.83 per LC case). The grand total for the disposable group, including sterilisation costs, was £574,706.25 (an average of £318.75 per LC case). Thus the saving for the trust using re-usable trocars, ports and clip applicators was £268.92 per case, £69,265.98 per annum and £484,861.84 over 7 years. CONCLUSIONS This study has demonstrated that considerable savings occur with a policy of minimal use of disposable equipment for LC. Using a disposable set, the instrument costs per procedure is 6.4 times greater than the cost of using re-usable LC sets. It behoves surgeons to be cost-effective and to reduce unnecessary expenditure and wastage. There is no evidence to support use of once-only laparoscopic instruments on grounds of patient safety, ease of use or transmission of infection. If the savings identified in this study of two surgeons' work (savings of £484,861.84 in a 7-year period) was extended not only across the hospital but across the NHS, large savings could be made for laparoscopic cholecystectomy. Even greater savings would accrue if the results were extrapolated to cover all laparoscopic surgery of whatever discipline.

scholarly journals Economic evaluation of disease elimination: An extension to the net-benefit framework and application to human African trypanosomiasis

2021 ◽  
Vol 118 (50) ◽  
pp. e2026797118
Author(s):  
Marina Antillon ◽  
Ching-I Huang ◽  
Kat S. Rock ◽  
Fabrizio Tediosi
Keyword(s):  
Human African Trypanosomiasis ◽  
Central Africa ◽  
Cost Savings ◽  
Cost Effective ◽  
African Trypanosomiasis ◽  
Net Benefit ◽  
Long Run ◽  
Health Community ◽  
Effective Use ◽  
The Cost

The global health community has earmarked a number of diseases for elimination or eradication, and these goals have often been praised on the premise of long-run cost savings. However, decision makers must contend with a multitude of demands on health budgets in the short or medium term, and costs per case often rise as the burden of a disease falls, rendering such efforts beyond the cost-effective use of scarce resources. In addition, these decisions must be made in the presence of substantial uncertainty regarding the feasibility and costs of elimination or eradication efforts. Therefore, analytical frameworks are necessary to consider the additional effort for reaching global goals, like elimination or eradication, that are beyond the cost-effective use of country resources. We propose a modification to the net-benefit framework to consider the implications of switching from an optimal strategy, in terms of cost-per-burden averted, to a strategy with a higher likelihood of meeting the global target of elimination or eradication. We illustrate the properties of our framework by considering the economic case of efforts to eliminate the transmission of gambiense human African trypanosomiasis (gHAT), a vector-borne, parasitic disease in West and Central Africa, by 2030.

scholarly journals Scaling diagnostics in times of COVID-19: Colorimetric Loop-mediated Isothermal Amplification (LAMP) assisted by a 3D-printed incubator for cost-effective and scalable detection of SARS-CoV-2

2020 ◽  
Author(s):  
Everardo González-González ◽  
Itzel Montserrat Lara-Mayorga ◽  
Iram Pablo Rodríguez-Sánchez ◽  
Felipe Yee-de León ◽  
Andrés García-Rubio ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Point Of Care ◽  
Three Dimensional ◽  
Cost Effective ◽  
Reaction Scheme ◽  
Ease Of Use ◽  
Isothermal Amplification ◽  
N Protein ◽  
Loop Mediated Isothermal Amplification ◽  
3D Printed

AbstractBy the third week of June 2020, more than 8,500,000 positive cases of COVID-19 and more than 450,000 deaths had been officially reported worldwide. The COVID-19 pandemic arrived in Latin America, India, and Africa—territories in which the mounted infrastructure for diagnosis is greatly underdeveloped. Here, we demonstrate the combined use of a three-dimensional (3D)-printed incubation chamber for commercial Eppendorf PCR tubes, and a colorimetric embodiment of a loop-mediated isothermal amplification (LAMP) reaction scheme for the detection of SARS-CoV-2 nucleic acids. We used this strategy to detect and amplify SARS-CoV-2 genetic sequences using a set of in-house designed initiators that target regions encoding the N protein. We were able to detect and amplify SARS-CoV-2 nucleic acids in the range of 62 to 2 × 105 DNA copies by this straightforward method. Using synthetic SARS-CoV-2 samples and a limited number of RNA extracts from patients, we also demonstrate that colorimetric LAMP is a quantitative method comparable in diagnostic performance to RT-qPCR. We envision that LAMP may greatly enhance the capabilities for COVID-19 testing in situations where RT-qPCR is not feasible or is unavailable. Moreover, the portability, ease of use, and reproducibility of this strategy make it a reliable alternative for deployment of point-of-care SARS-CoV-2 detection efforts during the pandemics.

scholarly journals Tanulók- és sportolók motoros-képességeinek fejlesztése és felmérése labdás játékok esetén

2021 ◽  
Author(s):  
Zsófia Keller ◽  
Róbert Szűcs
Keyword(s):  
Web Application ◽  
Cost Effective ◽  
Point Of View ◽  
Ease Of Use ◽  
Raspberry Pi ◽  
Physical Educators ◽  
Effective Implementation ◽  
The Right ◽  
The Cost ◽  
Object Frame

Kutatásunk célja egy olyan komplex hardver- szoftverrendszer létrehozása volt, amelynek segítségével megállapítható a technika elsajátításának hatékonysága labdás játékok esetén például egy kézilabdázó dobási pontosságának és dobóerejének mérése révén. A szemből és oldalnézetből készített felvételek alapján, a mozgó objektum képkockánkénti elmozdulásából számítható a labda sebessége és pozíciója a képelemzés eszközeinek segítségével. Különös figyelmet fordítottunk az eszköz költséghatékony megvalósítására, valamint fontosnak tartottuk az egyszerű kezelhetőséget a telepítés és a mérés folyamán egyaránt. Mindezt a megfelelő hardver és szoftver technológiák kiválasztásán túl az is biztosítja, hogy nem igényel külön hardver eszközt a kezelése, mivel az edzők vagy a testnevelők saját mobileszközükről férhetnek hozzá a rendszer szolgáltatásaihoz. Az egyszerű kezelhetőséget a telepítés és a mérés folyamán egyaránt szem előtt tartottuk. Ez a technológia mind módszertani szempontból, mind motivációs eszközként is figyelmet érdemel, akár edzéseken, akár az iskolai testnevelés órákon. A fenti szempontok figyelembevételével a Raspberry Pi 3B+, a hozzá csatlakozó Raspberry Pi Camera V2-es modelljére (Raspberry Pi) és az ESP32 Cam-ra (ESP32), mint hardver komponensekre esett a választásunk. A kezelőfelületet pedig egy webes alkalmazás biztosítja, amelyen keresztül megfelelő hitelesítési folyamatot követően elérhetők a rendszer szolgáltatásai. ----- Developement and measurement of ballplayers’ motoric skills ----- Our research aimed to create a complex hardware-software system that can be used to determine the effectiveness of the technique in ball games, for example by measuring the throwing accuracy and throwing power of a handball player. Based on the shots taken from the front and side views, the speed and position of the ball can be calculated from the displacement of the moving object frame by frame using image analysis tools. We paid special attention to the cost-effective implementation of the device, and we considered it important to be easy to use during both installation and measurement. In addition to selecting the right hardware and software technologies, this is ensured by the fact that it does not require a separate hardware device to manage, as coaches or physical educators can access the system's services from their mobile devices. Ease of use was kept in mind during both installation and measurement. This technology deserves attention both from a methodological point of view and as a motivational tool, whether in training or school physical education classes. Considering the above considerations, we chose the Raspberry Pi 3B+ with a Raspberry Pi Camera V2 model and the ESP32 Cam as hardware components. The user interface is provided by a web application through which the system services can be accessed after a proper authentication process.

The IBM-PC in electron microscopy

1996 ◽  
Vol 54 ◽  
pp. 612-613
Author(s):  
James F. Mancuso
Keyword(s):  
Head Start ◽  
Image Acquisition ◽  
Cost Effective ◽  
Financial Applications ◽  
Number Of Factors ◽  
Instrument Control ◽  
The Cost ◽  
Training And Support ◽  
Control Image ◽  
Ibm Pc

IBM PC compatible computers are widely used in microscopy for applications ranging from control to image acquisition and analysis. The choice of IBM-PC based systems over competing computer platforms can be based on technical merit alone or on a number of factors relating to economics, availability of peripherals, management dictum, or simple personal preference.IBM-PC got a strong “head start” by first dominating clerical, document processing and financial applications. The use of these computers spilled into the laboratory where the DOS based IBM-PC replaced mini-computers. Compared to minicomputer, the PC provided a more for cost-effective platform for applications in numerical analysis, engineering and design, instrument control, image acquisition and image processing. In addition, the sitewide use of a common PC platform could reduce the cost of training and support services relative to cases where many different computer platforms were used. This could be especially true for the microscopists who must use computers in both the laboratory and the office.

Sonographically guided, transcatheter foam sclerotherapy of the great saphenous vein

Phlebologie ◽  
2007 ◽  
Vol 36 (06) ◽  
pp. 309-312 ◽  
Author(s):  
T. Schulz ◽  
M. Jünger ◽  
M. Hahn
Keyword(s):  
Saphenous Vein ◽  
Great Saphenous Vein ◽  
Clinical Stage ◽  
Clinical Signs ◽  
Cost Effective ◽  
Duplex Sonography ◽  
Foam Sclerotherapy ◽  
Average Loss ◽  
One Year ◽  
The Cost

Summary Objective: The goal of the study was to assess the effectiveness and patient tolerability of single-session, sonographically guided, transcatheter foam sclerotherapy and to evaluate its economic impact. Patients, methods: We treated 20 patients with a total of 22 varicoses of the great saphenous vein (GSV) in Hach stage III-IV, clinical stage C2-C5 and a mean GSV diameter of 9 mm (range: 7 to 13 mm). We used 10 ml 3% Aethoxysklerol®. Additional varicoses of the auxiliary veins of the GSV were sclerosed immediately afterwards. Results: The occlusion rate in the treated GSVs was 100% one week after therapy as demonstrated with duplex sonography. The cost of the procedure was 207.91 E including follow-up visit, with an average loss of working time of 0.6 days. After one year one patient showed clinical signs of recurrent varicosis in the GSV; duplex sonography showed reflux in the region of the saphenofemoral junction in a total of seven patients (32% of the treated GSVs). Conclusion: Transcatheter foam sclerotherapy of the GSV is a cost-effective, safe method of treating varicoses of GSV and broadens the spectrum of therapeutic options. Relapses can be re-treated inexpensively with sclerotherapy.

A Digital Economy Model for Talent Prediction Data Analytic

2019 ◽  
Vol 2 (4) ◽  
pp. 260-266
Author(s):  
Haru Purnomo Ipung ◽  
Amin Soetomo
Keyword(s):  
Business Model ◽  
Industrial Revolution ◽  
Technology Innovation ◽  
Cost Effective ◽  
Sharing Economy ◽  
Token Economy ◽  
Economy Model ◽  
New Business ◽  
The Cost ◽  
Data Analytic

This research proposed a model to assist the design of the associated data architecture and data analytic to support talent forecast in the current accelerating changes in economy, industry and business change due to the accelerating pace of technological change. The emerging and re-emerging economy model were available, such as Industrial revolution 4.0, platform economy, sharing economy and token economy. Those were driven by new business model and technology innovation. An increase capability of technology to automate more jobs will cause a shift in talent pool and workforce. New business model emerge as the availabilityand the cost effective emerging technology, and as a result of emerging or re-emerging economic models. Both, new business model and technology innovation, create new jobs and works that have not been existed decades ago. The future workers will be faced by jobs that may not exist today. A dynamics model of inter-correlation of economy, industry, business model and talent forecast were proposed. A collection of literature review were conducted to initially validate the model.

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