Synthesis, characterization, antimicrobial, pesticidal and DNA cleavage activity of germanium(IV) derivatives of 3-(2-methyl-2,3-dihydro-benzthiazo-2-yl)-chromen-2-one and N′-[1-2-oxo-2H-chrome-3yl-ethylidene]-hydrazinecarbodithionic acid benzyl ester ligands

Abstract The new ligands 3-(2-methyl-2,3-dihydro-benzthiazo-2-yl)-chromen-2one (AcBzH) and N′-[1-2-oxo-2H-chrome-3yl-ethylidene]-hydrazinecarbodithionic acid benzyl ester (AcBDTZH) were prepared by the reaction of 3-acetyl-2H-chromen-2-one with 2-aminothiophenol and S-benzyl dithiocarbazate, respectively. The germanium(IV) complexes have been prepared by reacting Ph3GeCl and Me3GeCl in 1:1 molar ratio with these monofunctional bidentate AcBzH and AcBDTZH ligands by using microwave as well as conventional heating methods for comparison purposes. All the synthesized compounds were characterized by elemental analyses, melting point, IR, 1H-NMR 13C-NMR, mass and X-ray powder diffraction techniques. These studies showed that the ligands coordinated to the germanium atom in a monobasic bidentate manner and trigonal bipyramidal environment around the germanium atom have been established for the complexes. To evaluate the effect of metal ion upon chelation, both ligands and their complexes have been screened for their antimicrobial activity against the various pathogenic bacterial and fungal strains. The metal complexes have shown antimicrobial activity as compared to the free ligands. The pesticidal activity and DNA cleavage activity of both ligands and their metal complexes have been tested and discussed.

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Synthesis, Characterization, Antimicrobial, DNA Cleavage and Fluorescent Activity of Metal ion(II) Coordinate with 2H-Chromene Azo novel ligand

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v11i2.2116 ◽

2020 ◽

Vol 11 (2) ◽

pp. 1953-1960

Author(s):

Huda Muayad Nafea ◽

Ali Muayad Nafea Al- Kawaz

Keyword(s):

Dna Cleavage ◽

Metal Ion ◽

Condensation Reaction ◽

Free Ligand ◽

Cleavage Activity ◽

Dna Cleavage Activity ◽

Uv Visible ◽

Group 2 ◽

Conductance Measuring ◽

Cobalt And Nickel

A new ligand 2H-chromene containing azo group 2-(4-nitrophenyl)-N-(4-(phenyldiazenyl)-2H-chromen-4-amine (AH), were synthesized from the condensation reaction (1:2)of 2'-hydroxychalcone and p-aminoazobenzene. Co(II), Cu(II) and Ni(II) complexes of the new ligand have been synthesized and characterized using C.H.N. analysis,1HNMR spectra, FT.IR, UV/Visible, magnetic susceptible, conductance measuring, and fluorescence spectral spectroscopy; 13CNMR spectroscopy of the ligand was also studied. Spectroscopic results revealed the 2H-Chromene Azo (AH) ligand behaves as monodentate chelating via the nitrogen atom of amine groupat position 4 having 1:1 [M:L] ratio; suggested that the cobalt and nickel complexes have the tetrahedralstructure and a distorted tetrahedralgeometry for the copper complex, indicating their non-electrolyte nature. The new ligand shows a fluorescence emissioncomparingwith this fluorescence quenching was noticed in its metal complexes. The antibacterial potency of the free ligand and its chelates with metal ion(II) were screened against E.coli, K.pneumoniae, Staph.aureus andB.Subtilis;The DNA cleavage activity of the free ligand and its 2H-chromene azo metal (II) complexes was performedby the gel electrophoresis process, which giventhat thesecompounds are effectiveupon DNA cleavage .

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SYNTHESIS, CHARACTERISATION, THERMAL ANALYSIS AND DNA CLEAVAGE ACTIVITY OF A NOVELZINC (II) COMPLEX OF PYRAZOLE SCHIFF BASES

Kongunadu Research Journal ◽

10.26524/krj265 ◽

2018 ◽

Vol 5 (2) ◽

pp. 19-23

Author(s):

Jayanthi Eswaran

Keyword(s):

Schiff Base ◽

Schiff Base Ligand ◽

Dna Cleavage ◽

Metal Ion ◽

Acid Hydrazide ◽

Single Step ◽

Cleavage Activity ◽

Dna Cleavage Activity ◽

Shiff Base ◽

Ft Ir

A hydrazone Schiff base Zn(II) metal complex is synthesised from the Shiff base ligand Thiophene-2-carboxylic acid hydrazide and 1, 3-diphenyl-1H-pyrazole-4-carboxaldehyde reacted together in 1:1 mole ratio to obtain Schiff base ligand (HL) which was subsequently, allowed to react with Zn(CH3COO)2.2H2O. The Schiff base ligand and its Zn (II) complex prepared were characterized on the basis of elemental nalysis,thermogravimetry, UV-Visible spectroscopy, FT-IR spectroscopy and NMR spectroscopy. IR spectrum of the zinc complex shows that the ligand (HL) is coordinated to the metal ion in monoanionicbidentate fashion with the 1:2 metal to ligand stoichiometry. The thermal behaviour of thecomplex shows a single step decomposition pattern leaving the respective ZnO residue. The DNA cleavage activity of the complex ismonitored using agarose gel lectrophoresis method which indicates the potential of the complex to cleave supercoiled DNA.

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Synthesis, Characterization of Some Transition-Metal Complexes of a New Heptadentate N5S2 Schiff-Base Ligand and the Effects of These Metal Complexes on U2OS Cells Cytotoxicity and DNA Cleavage Activity

Phosphorus Sulfur and Silicon and the Related Elements ◽

10.1080/10426500801907589 ◽

2008 ◽

Vol 183 (9) ◽

pp. 2237-2247 ◽

Cited By ~ 3

Author(s):

G. Uluçcam ◽

N. Beynek ◽

Z. Seller ◽

G. Akalın ◽

G. Turan ◽

...

Keyword(s):

Schiff Base ◽

Transition Metal ◽

Metal Complexes ◽

Transition Metal Complexes ◽

Schiff Base Ligand ◽

Dna Cleavage ◽

Cleavage Activity ◽

Dna Cleavage Activity ◽

U2os Cells

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Indirect Readout of DNA Controls Filamentation and Activation of a Sequence-Specific Endonuclease

10.1101/585943 ◽

2019 ◽

Author(s):

Smarajit Polley ◽

Dmitry Lyumkis ◽

N. C. Horton

Keyword(s):

Conformational Changes ◽

Dna Cleavage ◽

Metal Ion ◽

Structural Changes ◽

Enzyme Mechanism ◽

Sequence Specificity ◽

Dna Sequence Specificity ◽

Cleavage Activity ◽

Dna Cleavage Activity ◽

Indirect Readout

ABSTRACTFilament or run-on oligomer formation by enzymes is increasingly recognized as an important phenomenon with potentially unique regulatory properties and biological roles. SgrAI is an allosterically regulated type II restriction endonuclease that forms run-on oligomeric (ROO) filaments with enhanced DNA cleavage activity and altered sequence specificity. Here, we present the 3.5 Å cryo-electron microscopy structure of the ROO filament of SgrAI bound to a mimic of cleaved primary site DNA and Mg2+. Large conformational changes stabilize a second metal ion cofactor binding site within the catalytic pocket and facilitate assembling a higher-order enzyme form that is competent for rapid DNA cleavage. The structural changes illuminate the mechanistic origin of hyper-accelerated DNA cleavage activity within the filamentous SgrAI form. An analysis of the protein-DNA interface and the stacking of individual nucleotides reveals how indirect DNA readout within filamentous SgrAI enables recognition of substantially more nucleotide sequences than its low-activity form, thereby expanding DNA sequence specificity. Together, substrate DNA binding, indirect readout, and filamentation simultaneously enhance SgrAI’s catalytic activity and modulate substrate preference. This unusual enzyme mechanism may have evolved to perform the specialized functions of bacterial innate immunity in rapid defense against invading phage DNA without causing damage to the host DNA.

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Syntheses, Characterization, Antimicrobial and DNA Cleavage Activity of Cr(III) Complexes with Coumarin based Schiff Base Ligands

Asian Journal of Chemistry ◽

10.14233/ajchem.2021.23431 ◽

2021 ◽

Vol 33 (12) ◽

pp. 2983-2988

Author(s):

Ramhari Meena ◽

Anita Kumari ◽

Naveen Sharma ◽

Nighat Fahmi

Keyword(s):

Schiff Base ◽

Dna Cleavage ◽

Metal Ion ◽

Biological Activities ◽

Spectroscopic Techniques ◽

Molecular Weight Determination ◽

Central Metal ◽

Schiff Base Ligands ◽

Cleavage Activity ◽

Dna Cleavage Activity

A series of biologically important complexes of chromium(III) have been synthesized by the reaction of 3-formyl-4-chlorocoumarin hydrazinecarbothioamide (L1H) and 3-formyl-4-chlorocoumarin hydrazinecarboxamide (L2H) with CrCl3·6H2O in 1:1 and 1:2 molar ratio. All the complexes have been characterized by elemental analysis, molecular weight determination, melting point, conductivity measurements, electronic, IR, 1H NMR, 13C NMR and EPR spectroscopic techniques and X-ray diffraction. In vitro biological screening effects of the compounds were tested against the pathogenic bacterial and fungal species. Further, free ligands and their metal complexes have been screened for their DNA cleavage activity. A comparative study of the biological activities of the Schiff base ligands and their Cr(III) complexes indicates that the complexes exhibit higher antimicrobial and DNA cleavage activity than the free ligands. Physico-chemical studies and spectral data suggested a hexa-coordinated environment around the central metal ion.

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Synthesis, Structural Characterization, and Biological Activity Studies of Ni(II) and Zn(II) Complexes

Bioinorganic Chemistry and Applications ◽

10.1155/2014/568741 ◽

2014 ◽

Vol 2014 ◽

pp. 1-13 ◽

Cited By ~ 3

Author(s):

Palakuri Kavitha ◽

K. Laxma Reddy

Keyword(s):

Metal Complexes ◽

Dna Cleavage ◽

Spectroscopic Data ◽

Nuclease Activity ◽

Molar Conductance ◽

Agarose Gel Electrophoresis ◽

Tridentate Ligands ◽

Cleavage Activity ◽

Dna Cleavage Activity ◽

Bacteria And Fungi

Ni(II) and Zn(II) complexes were synthesized from tridentate 3-formyl chromone Schiff bases such as 3-((2-hydroxyphenylimino)methyl)-4H-chromen-4-one (HL1), 2-((4-oxo-4H-chromen-3-yl)methylneamino)benzoic acid (HL2), 3-((3-hydroxypyridin-2-ylimino)methyl)-4H-chromen-4-one (HL3), and 3-((2-mercaptophenylimino)methyl)-4H-chromen-4-one (HL4). All the complexes were characterized in the light of elemental analysis, molar conductance, FTIR, UV-VIS, magnetic, thermal, powder XRD, and SEM studies. The conductance and spectroscopic data suggested that, the ligands act as neutral and monobasic tridentate ligands and form octahedral complexes with general formula [M(L1–4)2]·nH2O (M = Ni(II) and Zn(II)). Metal complexes exhibited pronounced activity against tested bacteria and fungi strains compared to the ligands. In addition metal complexes displayed good antioxidant and moderate nematicidal activities. The cytotoxicity of ligands and their metal complexes have been evaluated by MTT assay. The DNA cleavage activity of the metal complexes was performed using agarose gel electrophoresis in the presence and absence of oxidant H2O2. All metal complexes showed significant nuclease activity in the presence of H2O2.

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DNA-cleavage activity of the iron(II) complex with optically active ligands, meta- and para-xylyl-linked N’,N’-dipyridylmethyl-cyclohexane-1,2-diamine

Bioorganic & Medicinal Chemistry Letters ◽

10.1016/j.bmcl.2021.127834 ◽

2021 ◽

Vol 36 ◽

pp. 127834

Author(s):

Koichi Kato ◽

Yoshimi Ichimaru ◽

Yoshinori Okuno ◽

Yoshihiro Yamaguchi ◽

Wanchun Jin ◽

...

Keyword(s):

Dna Cleavage ◽

Optically Active ◽

Cleavage Activity ◽

Dna Cleavage Activity

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A YoeB toxin cleaves both RNA and DNA

Scientific Reports ◽

10.1038/s41598-021-82950-6 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Julia McGillick ◽

Jessica R. Ames ◽

Tamiko Murphy ◽

Christina R. Bourne

Keyword(s):

Dna Cleavage ◽

Divalent Cations ◽

Nuclease Activity ◽

Dose Dependence ◽

Cleavage Activity ◽

Double Stranded Dna ◽

Dna Cleavage Activity ◽

Evolutionarily Conserved ◽

Toxin Protein ◽

Rna And Dna

AbstractType II toxin-antitoxin systems contain a toxin protein, which mediates diverse interactions within the bacterial cell when it is not bound by its cognate antitoxin protein. These toxins provide a rich source of evolutionarily-conserved tertiary folds that mediate diverse catalytic reactions. These properties make toxins of interest in biotechnology applications, and studies of the catalytic mechanisms continue to provide surprises. In the current work, our studies on a YoeB family toxin from Agrobacterium tumefaciens have revealed a conserved ribosome-independent non-specific nuclease activity. We have quantified the RNA and DNA cleavage activity, revealing they have essentially equivalent dose-dependence while differing in requirements for divalent cations and pH sensitivity. The DNA cleavage activity is as a nickase for any topology of double-stranded DNA, as well as cleaving single-stranded DNA. AtYoeB is able to bind to double-stranded DNA with mid-micromolar affinity. Comparison of the ribosome-dependent and -independent reactions demonstrates an approximate tenfold efficiency imparted by the ribosome. This demonstrates YoeB toxins can act as non-specific nucleases, cleaving both RNA and DNA, in the absence of being bound within the ribosome.

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