scholarly journals Synthesis, characterization and anticancer activities of silver nanoparticles from the leaves of Datura stramonium L.

2021 ◽  
Vol 6 (1) ◽  
pp. 25-35
Author(s):  
Gourav Chandan ◽  
Soumya Pal ◽  
Sheetal Kashyap ◽  
Samarjeet Singh Siwal ◽  
Shakti K. Dhiman ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Cell Lines ◽  
Mammalian Cell ◽  
Present Report ◽  
Datura Stramonium ◽  
Anticancer Activities ◽  
Ag Nps ◽  
Mammalian Cell Lines ◽  
Sem And Tem ◽  
Wide Range

Abstract In recent years, a wide range of studies has pointed out the role of nanoparticles as reservoirs of therapeutics for several diseases, including cancer. Nowadays, cancer research is focused on the development of novel treatment approaches to fight this dreadful disorder. Based on the evidential research and applications of nanoparticles, it is expected that green synthesized nanoparticles may show a prominent role, especially in the biomedical field. The present work is centered on the preparation and characterization of silver nanoparticles (Ag-NPs) from the aqueous (AQ) extract and non-alkaloidal (NA) fraction of Datura stramonium leaves and to evaluate their anticancer potential against mammalian cell lines. The biogenic Ag-NPs are characterized by UV-vis spectra, FTIR DLS, UV-Vis, SEM, and TEM. SEM and TEM analysis reveals the spherical morphology of NPs. The Ag-NPs exhibit cytotoxicity against various mammalian cell lines (A549, HCT-116, PANC-1, SHSY5Y, and U87), which indicate that the AQ and NA based NPs are highly potent to cause cancer cell death. To the best of our knowledge, the present report, for the first time, describes the green synthesis of Ag-NPs from the NA fraction of the D. stramonium and provides pieces of evidence for its anticancer potential.

scholarly journals Structure-guided selection of puromycin N-acetyltransferase mutants with enhanced selection stringency for deriving mammalian cell lines expressing recombinant proteins

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Alessandro T. Caputo ◽  
Oliver M. Eder ◽  
Hana Bereznakova ◽  
Heleen Pothuis ◽  
Albert Ardevol ◽  
...  
Keyword(s):  
Cell Lines ◽  
Mammalian Cell ◽  
Recombinant Proteins ◽  
Cultured Cells ◽  
Hek293 Cells ◽  
Reaction Products ◽  
Enzyme Form ◽  
X Ray Crystallography ◽  
Mammalian Cell Lines ◽  
Apparent Loss

AbstractPuromycin and the Streptomyces alboniger-derived puromycin N-acetyltransferase (PAC) enzyme form a commonly used system for selecting stably transfected cultured cells. The crystal structure of PAC has been solved using X-ray crystallography, revealing it to be a member of the GCN5-related N-acetyltransferase (GNAT) family of acetyltransferases. Based on structures in complex with acetyl-CoA or the reaction products CoA and acetylated puromycin, four classes of mutations in and around the catalytic site were designed and tested for activity. Single-residue mutations were identified that displayed a range of enzymatic activities, from complete ablation to enhanced activity relative to wild-type (WT) PAC. Cell pools of stably transfected HEK293 cells derived using two PAC mutants with attenuated activity, Y30F and A142D, were found to secrete up to three-fold higher levels of a soluble, recombinant target protein than corresponding pools derived with the WT enzyme. A third mutant, Y171F, appeared to stabilise the intracellular turnover of PAC, resulting in an apparent loss of selection stringency. Our results indicate that the structure-guided manipulation of PAC function can be utilised to enhance selection stringency for the derivation of mammalian cell lines secreting elevated levels of recombinant proteins.

scholarly journals Reactivity and Chemical Sintering of Carey Lea Silver Nanoparticles

Nanomaterials ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 1525
Author(s):  
Sergey Vorobyev ◽  
Elena Vishnyakova ◽  
Maxim Likhatski ◽  
Alexander Romanchenko ◽  
Ivan Nemtsev ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Silver Nanoparticles ◽  
Flexible Electronics ◽  
Photoelectron Spectroscopy ◽  
Colloidal Solutions ◽  
Ag Nps ◽  
Wide Range ◽  
Carboxylic Groups ◽  
The Media ◽  
Sulfide Ions

Carey Lea silver hydrosol is a rare example of very concentrated colloidal solutions produced with citrate as only protective ligands, and prospective for a wide range of applications, whose properties have been insufficiently studied up to now. Herein, the reactivity of the immobilized silver nanoparticles toward oxidation, sulfidation, and sintering upon their interaction with hydrogen peroxide, sulfide ions, and chlorocomplexes of Au(III), Pd(II), and Pt(IV) was investigated using SEM and X-ray photoelectron spectroscopy (XPS). The reactions decreased the number of carboxylic groups of the citrate-derived capping and promoted coalescence of 7 nm Ag NPs into about 40 nm ones, excluding the interaction with hydrogen peroxide. The increased nanoparticles form loose submicrometer aggregates in the case of sulfide treatment, raspberry-like micrometer porous particles in the media containing Pd(II) chloride, and densely sintered particles in the reaction with inert H2PtCl6 complexes, probably via the formation of surface Ag-Pt alloys. The exposure of Ag NPs to HAuCl4 solution produced compact Ag films along with nanocrystals of Au metal and minor Ag and AgCl. The results are promising for chemical ambient temperature sintering and rendering silver-based nanomaterials, for example, for flexible electronics, catalysis, and other applications.

RAS p21 and other Gn proteins are detected in mammalian cell lines by [γ-35S]GTPγS binding

1989 ◽  
Vol 159 (3) ◽  
pp. 1269-1274 ◽  
Author(s):  
J.G. Comerford ◽  
J.R. Gibson ◽  
A.P. Dawson ◽  
I. Gibson
Keyword(s):  
Cell Lines ◽  
Mammalian Cell ◽  
Mammalian Cell Lines ◽  
Gtpγs Binding ◽  
Ras P21
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