Serum amyloid A protein (SAA), haptoglobin (Hp) and selected hematological and biochemical parameters in wild mares before and after parturition

2017 ◽  
Vol 20 (2) ◽  
pp. 299-305 ◽  
Author(s):  
L. Krakowski ◽  
P. Bartoszek ◽  
I. Krakowska ◽  
P. Olcha ◽  
T. Piech ◽  
...  
Keyword(s):  
Acute Phase ◽  
Biochemical Parameters ◽  
Acute Phase Proteins ◽  
Perinatal Period ◽  
Serum Concentrations ◽  
Amyloid A ◽  
Group I ◽  
Significant Difference ◽  
Group Ii ◽  
Hematological And Biochemical Parameters

Abstract The aim of the study was to evaluate physiological changes in hematological and biochemical parameters in mares in perinatal period. Blood samples were collected from 24 pregnant Polish Konik breed mares which were divided into two groups. The first group (Group – I, n=12) comprised mares living in the wild, in the reserve. The second group (Group – II, n=12) consisted of mares kept in stables. The blood was collected 2 weeks prior to the parturition, then 24 hours after the delivery, and then at the 7th and 21st day after foaling. When comparing the two groups before the parturition, no significant differences in terms of WBC, RBC, and Hb were found, however, there was a significant difference in MCV, MCH, LYM, NEU and SEG NEU (p≤0.05). In Group II, 24 hours after the parturition and at the 21st day after foaling, a significant raise in WBC, NEU and SEG NEU (p≤0.05) was detected. No significant differences in serum concentrations of proteins such as TP, Alb or Glb were observed. As to acute phase proteins, significant rise in SAA and Hp (p≤0.05) was found in the two examined groups 24 hours after the parturition. Yet, this rise remained within physiological range. The study revealed a certain degree of fluctuations in hematological parameters, in serum concentrations of acute-phase proteins and total proteins in the mares in the perinatal period. However, these changes remained still within physiological ranges and thus they do not indicate potential susceptibility to disorders of perinatal period.

scholarly journals The diagnostic accuracy of acute phase proteins and proinflammatory cytokines in sheep with pneumonic pasteurellosis

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e2161 ◽  
Author(s):  
Wael M. El-Deeb ◽  
Ahmed M. Elmoslemany
Keyword(s):  
Diagnostic Accuracy ◽  
Acute Phase ◽  
Proinflammatory Cytokines ◽  
Diagnostic Performance ◽  
Pasteurella Multocida ◽  
Acute Phase Proteins ◽  
Eastern Region ◽  
Pneumonic Pasteurellosis ◽  
Amyloid A ◽  
Significant Difference

The goal of this study was to assess the diagnostic accuracy of acute phase proteins and proinflammatory cytokines in sheep with pneumonic pasteurellosis. Blood samples were collected from 56 sheep (36 naturally infected withPasteurella multocidaand 20 healthy controls) belonging to one farm in Eastern region, Saudi Arabia. Serum samples were evaluated for acute phase proteins (Haptoglobin (Hp), serum amyloid A (SAA) and fibrinogen (Fb)), and the proinflammatory cytokines (interleukins (IL-1α, IL-1β, and IL-6), tumor necrosis factor-alpha (TNF-α), and interferon-gamma (IFN-ϒ)). Additionally, nasopharyngeal swabs and bronchoalveolar lavages were collected from all animals for bacteriological examinations. Receiver operating characteristic curve was used to assess the diagnostic performance of each parameter. All parameters showed moderate to high degree of positive correlation with case-control status. There was no significant difference in the area under the curve (AUC) among acute phase proteins; however, both Hp and SAA showed better sensitivity and specificity than Fb. The proinflammatory cytokines (IL1-α, IL1-β, and IL6) showed similar and highly accurate diagnostic performance (AUC> 0.9), whereas IFN-ϒ was moderately accurate (AUC= 0.79). In conclusion, this study confirms the value of acute phase proteins and cytokines as diagnostic biomarkers of naturally occuring pneumonic pasteurellosis in sheep.

scholarly journals ACUTE PHASE PROTEIN INCREASE IN HIGH ALTITUDE MOUNTAINEERS

2017 ◽  
Vol 23 (6) ◽  
pp. 455-459
Author(s):  
Tolga Saka ◽  
Mehmet Karakus
Keyword(s):  
High Altitude ◽  
Acute Phase ◽  
Biochemical Parameters ◽  
Creatine Phosphokinase ◽  
Acute Phase Proteins ◽  
Acute Phase Protein ◽  
Serum Levels ◽  
Mountain Climbing ◽  
Sedentary Group ◽  
Hematological And Biochemical Parameters

ABSTRACT Introduction: Many middle-aged Turks go hiking in mountains to breathe some fresh air or to maintain fitness. Objective: This study investigated the effects of regular high altitude mountain climbing on the metabolic and hematological responses of mountaineers. Methods: Hematological and biochemical parameters were studied, as well as some hormonal values of 21 mountaineers and 16 healthy age-matched sedentary volunteers. Results: The neutrophil to lymphocyte ratio (NLR) was significantly lower (p<0.04) in mountaineers compared with the sedentary group. Total protein (p<0.001) and albumin (p<0.001) were lower, while the levels of ferritin (p<0.04), creatine (p<0.03) and creatine phosphokinase (p<0.01) were higher in mountaineers. Other hematological and biochemical parameters, i.e., erythrocytes, leukocytes, hemoglobin and hematocrit, did not change significantly. Conclusion: Our results show that regular exposure to high altitude increased the serum levels of some acute phase proteins with anti-inflammatory properties.

Effectiveness of a Gentamicin Dosing Protocol Based on Postconceptional Age: Comparison to Published Neonatal Guidelines

1992 ◽  
Vol 26 (4) ◽  
pp. 534-538 ◽  
Author(s):  
Michael D. Reed ◽  
Ana M. Lopez-Samblas ◽  
Carmen L. Torres ◽  
Helena Wang ◽  
William J. Feuer ◽  
...  
Keyword(s):  
Medical Center ◽  
Pharmacokinetic Parameters ◽  
Pharmacokinetic Data ◽  
Serum Concentrations ◽  
Group I ◽  
Trough Serum ◽  
Significant Difference ◽  
Postconceptional Age ◽  
Trough Concentrations ◽  
Group Ii

OBJECTIVE: To evaluate the effectiveness of a gentamicin dosing protocol based on postconceptional age in producing therapeutic serum concentrations and to compare the protocol with commonly used gentamicin dosing guidelines. DESIGN: During the initial three months of this study infants were dosed according to physician discretion (group I). In the subsequent three-month period patients were dosed according to a postconceptional age dosing schedule (group II). SETTING: Infants were enrolled after being admitted to the Newborn Intensive Care Unit at the University of Miami/Jackson Memorial Medical Center. PATIENTS: Infants less than 37 weeks gestational age with normal renal function, not receiving indomethacin, and requiring gentamicin treatment were enrolled. Fifty-nine infants were enrolled into group I (median weight 1300 g [range 720–3300]), postconceptional age 29 weeks [26–37]); and 68 infants were enrolled into group II (weight 970 g [530–3000], postconceptional age 29 weeks [24–36]). INTERVENTION: Patients in group II were dosed according to the following protocol: postconceptional age <30 weeks, 3.0 mg/kg q24h, and postconceptional age 30–37 weeks, 2.5 mg/kg q18h. Peak and trough serum gentamicin concentrations were obtained in all study patients. Pharmacokinetic parameters were calculated from measured serum concentrations. Using the calculated pharmacokinetic data, peak and trough serum concentrations were simulated for five published neonatal dosing guidelines and the proposed postconceptional age protocol. MAIN OUTCOME MEASURES: The number of therapeutic serum gentamicin concentrations resulting from the dosing guidelines studied were compared. RESULTS: Measured trough concentrations differed significantly between the two groups with 35 percent of patients in group I and 90 percent of patients in group II having trough values <2 mg/L (p<0.001). There was no significant difference in measured peak concentrations between groups. Simulated trough concentrations were significantly different when postconceptional age dosing was compared with commonly used protocols (p<0.0001) with the highest percentage of concentrations <2 mg/L (89 percent) resulting from the proposed postconceptional age guidelines. CONCLUSIONS: These data suggest that the proposed postconceptional age protocol is reproducible and reliable in achieving therapeutic gentamicin serum concentrations in neonates.

scholarly journals Effects of selected nonsteroidal anti-inflammatory drugs on acute-phase protein levels after dehorning in Holstein heifers

2019 ◽  
Vol 75 (05) ◽  
pp. 6265-2019
Author(s):  
HASAN ERDOGAN ◽  
IBRAHIM AKIN ◽  
KEREM URAL ◽  
PINAR ALKIM ULUTAS
Keyword(s):  
Acute Phase ◽  
Serum Amyloid A ◽  
Acute Phase Proteins ◽  
Serum Amyloid ◽  
Mean Values ◽  
Protein Levels ◽  
Amyloid A ◽  
Flunixin Meglumine ◽  
Significant Difference ◽  
Inflammatory Drugs

The purpose of this study was to evaluate the effects of ketoprofen (KTP), flunixin meglumine (FLM), and meloxicam (MLX) administration on acute-phase proteins after dehorning in Holstein heifers. A total of 21 Holstein heifers were enrolled into three groups of equal size (n=7) and administered ketoprofen, flunixin meglumine, or meloxicam, at doses of 2.2 mg/kg, 1.1 mg/kg, and 1 mg/kg body weight, respectively. Serum amyloid A, haptoglobin, and ceruloplasmin levels were determined before the administration of the three drugs (0 hrs) and at 6, 12, 24, 48, and 96 hours post-administration. The mean values (±SD) obtained revealed no significant alteration in APP levels at 0 hrs in any of the three groups. Time-dependent alterations, however, were significant in all groups. Group-time interactions were significant (P < 0.001) for ceruloplasmin concentrations, whereas results for serum amyloid A and haptoglobin levels were deemed non-significant. Inter-group interaction revealed no significant findings regarding serum amyloid A and ceruloplasmin levels, but haptoglobin levels showed a significant difference between the KTP and FLM groups at 48 hrs. It may therefore be reasonably suggested that KTP, FLM, and MLX could all be administered to effect slight changes in acute phase proteins.

scholarly journals Serum concentrations of some acute phase proteins in cats with anaemia

2018 ◽  
Vol 74 (1) ◽  
pp. 5998-2018
Author(s):  
GÜLTEN EMEK TUNA ◽  
CEREN DINLER ◽  
GAMZE SEVRI EKREN AŞICI ◽  
BÜLENT ULUTAŞ
Keyword(s):  
Haemolytic Anaemia ◽  
Acute Phase ◽  
Serum Amyloid A ◽  
Acute Phase Proteins ◽  
Serum Amyloid ◽  
Serum Concentrations ◽  
Healthy Group ◽  
Acid Glycoprotein ◽  
Amyloid A ◽  
Serum Haptoglobin

Serum concentrations of acute phase proteins can provide valuable diagnostic information in the detection and monitoring of disease. The available information on the acute phase response in cats with anaemia is limited. The aim of this study was to retrospectively evaluate serum concentrations of haptoglobin, serum amyloid A, α1 acid glycoprotein and their clinical importance in cats with anaemia. Thirty-four anaemic cats and ten healthy cats were enrolled this study. After individual diagnoses had been established, the cats were divided into three groups (healthy group, haemolytic group and non-haemolytic group). Serum acute phase protein concentrations were analysed using specific commercially available test kits in an ELISA reader device. Serum amyloid A and serum α1 acid glycoprotein concentrations were significantly higher in the anaemic groups compared with the healthy group. Haptoglobin concentrations were significantly higher in cats from the non-haemolytic anaemia group than they were in healthy animals and those from the haemolytic anaemia group. Although serum haptoglobin concentrations were lower than in the healthy group, there was no significant difference between the haemolytic anaemia group and the healthy group. The results of this study suggest that serum amyloid A and α1 acid glycoprotein could be useful in the diagnosis and determination of inflammation in cats with anaemia. Serum haptoglobin depletion may be used for diagnosis of haemolysis in cats with haemolytic anaemia. In addition, this study has contributed to the limited data available on acute phase protein concentrations in cats with anaemia..

scholarly journals Comparison of host endothelial, epithelial and inflammatory response in ICU patients with and without COVID-19: a prospective observational cohort study

Critical Care ◽  
2021 ◽  
Vol 25 (1) ◽  
Author(s):  
Pavan K. Bhatraju ◽  
Eric D. Morrell ◽  
Leila Zelnick ◽  
Neha A. Sathe ◽  
Xin-Ya Chai ◽  
...  
Keyword(s):  
Endothelial Dysfunction ◽  
Epithelial Cell ◽  
Acute Phase ◽  
Cell Injury ◽  
Acute Phase Proteins ◽  
Invasive Mechanical Ventilation ◽  
Icu Patients ◽  
Icu Admission ◽  
Amyloid A ◽  
Epithelial Cell Injury

Abstract Background Analyses of blood biomarkers involved in the host response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral infection can reveal distinct biological pathways and inform development and testing of therapeutics for COVID-19. Our objective was to evaluate host endothelial, epithelial and inflammatory biomarkers in COVID-19. Methods We prospectively enrolled 171 ICU patients, including 78 (46%) patients positive and 93 (54%) negative for SARS-CoV-2 infection from April to September, 2020. We compared 22 plasma biomarkers in blood collected within 24 h and 3 days after ICU admission. Results In critically ill COVID-19 and non-COVID-19 patients, the most common ICU admission diagnoses were respiratory failure or pneumonia, followed by sepsis and other diagnoses. Similar proportions of patients in both groups received invasive mechanical ventilation at the time of study enrollment. COVID-19 and non-COVID-19 patients had similar rates of acute respiratory distress syndrome, severe acute kidney injury, and in-hospital mortality. While concentrations of interleukin 6 and 8 were not different between groups, markers of epithelial cell injury (soluble receptor for advanced glycation end products, sRAGE) and acute phase proteins (serum amyloid A, SAA) were significantly higher in COVID-19 compared to non-COVID-19, adjusting for demographics and APACHE III scores. In contrast, angiopoietin 2:1 (Ang-2:1 ratio) and soluble tumor necrosis factor receptor 1 (sTNFR-1), markers of endothelial dysfunction and inflammation, were significantly lower in COVID-19 (p < 0.002). Ang-2:1 ratio and SAA were associated with mortality only in non-COVID-19 patients. Conclusions These studies demonstrate that, unlike other well-studied causes of critical illness, endothelial dysfunction may not be characteristic of severe COVID-19 early after ICU admission. Pathways resulting in elaboration of acute phase proteins and inducing epithelial cell injury may be promising targets for therapeutics in COVID-19.

scholarly journals Effects of Stocking and Transport Conditions on Physicochemical Properties of Meat and Acute-Phase Proteins in Cattle

Foods ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 252
Author(s):  
Ahmed A. Abubakar ◽  
Idrus Zulkifli ◽  
Yong M. Goh ◽  
Ubedullah Kaka ◽  
Azad B. Sabow ◽  
...  
Keyword(s):  
Physicochemical Properties ◽  
Acute Phase ◽  
Acute Phase Proteins ◽  
Stocking Density ◽  
Road Transport ◽  
Long Distance ◽  
Amyloid A ◽  
Tropical Conditions ◽  
Meat Samples ◽  
Water Holding

This study’s objective was to evaluate the effects of distance and stocking density on physicochemical properties and oxidative stability of meat and acute-phase proteins in Brahman crossbred cattle transported by road under hot and humid tropical conditions. Sixty Brahman crossbred heifers were subjected to road transport from a cattle feedlot farm located in Universiti Putra Malaysia (UPM), Serdang, to a commercial ruminant abattoir in Shah Alam, Selangor. Animals were assigned to long and short distances and high, medium, and low stocking densities. The results revealed that the intensity of response significantly increased in meat samples from animals subjected to long-distance transportation and higher stocking density. Alpha-1-acid glycoprotein and serum amyloid-A values increased considerably and were different from the baseline values recorded at preload. In conclusion, the current results revealed that the color, pH, shear force values, water holding capacity (WHC), glycogen level, and malondilaldehyde assay (MDA) concentrations in meat and acute-phase proteins (APP) were affected by both distances and stocking densities, as evidenced by the significant changes recorded from the parameters above.

scholarly journals Impact of hemodialysis on the wellbeing of chronic kidney diseases patients: a pre-post analysis

2020 ◽  
Vol 27 (1) ◽  
Author(s):  
Um-e-Kalsoom ◽  
Sabiha Khan ◽  
Israr Ahmad
Keyword(s):  
Quality Of Life ◽  
Social Support ◽  
Perceived Social Support ◽  
Kidney Diseases ◽  
Chronic Kidney Diseases ◽  
Group I ◽  
Significant Difference ◽  
Group Ii ◽  
The Impact

Abstract Background Hemodialysis may have serious psychological impact upon patients suffering from chronic kidney diseases. The aim of the present study is to investigate the impact of hemodialysis on the wellbeing of individuals with chronic kidney diseases (CKD). Result A sample consists of (N = 100) CKD patients referred from neurology ward of Leady Reading Hospital Peshawar. Data was collected from both male (50%) and female (50%) in 2017. Participants were divided into two groups on the basis of pre-set criteria. In group I, individuals with 4–5 stage of CKD referred first time for dialysis treatment were recruited. Group II comprised of CKD patients with 1–3 stage. Demographic data sheet, Pakistan Anxiety and Depression, WHO Quality of Life scale, and Perceived Social support scale (PSS) were used to test the hypotheses. Paired sample t test was use to see the difference between pre- and post-analysis of depression, anxiety, QOL, and PSS in group I (experimental group). Results suggests significant difference on depression (p > .001), anxiety (p > .001), and QOL (p > .001), while no significant difference was reported on perceived social support (p <.673). Findings also indicate no significant difference between group I and group II on QOL depression, anxiety, and PSS. Conclusion The findings concluded that patients under hemodialysis treatment suffered from depression, anxiety, and poor quality of life.

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