Non-invasive detection of free hemoglobin in red blood cell concentrates for quality assurance

2015 ◽  
Vol 4 (2) ◽  
Author(s):  
Uwe J. Netz ◽  
Lesley Hirst ◽  
Moritz Friebel
Keyword(s):  
Quality Control ◽  
Quality Assurance ◽  
Blood Cell ◽  
Blood Cells ◽  
Measurement Method ◽  
Hemoglobin Concentration ◽  
Red Cell ◽  
Free Hemoglobin ◽  
Non Invasive ◽  
Invasive Measurement

AbstractThere is currently no non-invasive measurement method available on the market for the quality control of red cell concentrates (RCCs). As the level of free hemoglobin is an indicator for hemolysis resulting from destroyed or overaged red blood cells, it is an important parameter to assess the quality and usability of RCCs before transfusion. A new optical device has been developed and tested to enable the measurement of the free hemoglobin concentration non-invasively in RCCs.

Footstrike is the major cause of hemolysis during running

2003 ◽  
Vol 94 (1) ◽  
pp. 38-42 ◽  
Author(s):  
R. D. Telford ◽  
G. J. Sly ◽  
A. G. Hahn ◽  
R. B. Cunningham ◽  
C. Bryant ◽  
...  
Keyword(s):  
Oxygen Uptake ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Random Order ◽  
Peak Oxygen Uptake ◽  
Red Cell ◽  
Free Hemoglobin ◽  
Serum Haptoglobin ◽  
Total Hemoglobin ◽  
Exercise Induced

There is a wide body of literature reporting red cell hemolysis as occurring after various forms of exercise. Whereas the trauma associated with footstrike is thought to be the major cause of hemolysis after running, its significance compared with hemolysis that results from other circulatory stresses on the red blood cell has not been thoroughly addressed. To investigate the significance of footstrike, we measured the degree of hemolysis after 1 h of running. To control for the potential effects of oxidative and circulatory stresses on the red blood cell, the same subjects cycled for 1 h at equivalent oxygen uptake. Our subjects were 10 male triathletes, who each completed two separate 1-h sessions of running and cycling at 75% peak oxygen uptake, which were performed in random order 1 wk apart. Plasma free hemoglobin and serum haptoglobin concentrations were measured as indicators of hemolysis. We also measured methemoglobin as a percentage of total hemoglobin immediately postexercise as an indicator of red cell oxidative stress. Plasma free hemoglobin increased after both running ( P < 0.01) and cycling ( P < 0.01), but the increase was fourfold greater after running ( P < 0.01). This was reflected by a significant fall in haptoglobin 1 h after the running trials, whereas no significant changes occurred after cycling at any sample point. Methemoglobin increased twofold after both running and cycling ( P < 0.01), with no significant differences between modes of exercise. The present data indicate that, whereas general circulatory trauma to the red blood cells associated with 1 h of exercise at 75% maximal oxygen uptake may result in some exercise-induced hemolysis, footstrike is the major contributor to hemolysis during running.

Effects of Thyroid Function on Blood Cell Counts and Red Cell Indices – A Retrospective Study at a Tertiary Care Centre in Mandya, Karnataka

2021 ◽  
Vol 8 (27) ◽  
pp. 2434-2438
Author(s):  
Siddegowda M.S ◽  
Chaithra R ◽  
Shivakumar S ◽  
Maithri C.M
Keyword(s):  
Blood Cell ◽  
Blood Cells ◽  
Thyroid Dysfunction ◽  
Haematological Parameters ◽  
Red Cell ◽  
Corpuscular Haemoglobin ◽  
Significant Difference ◽  
Red Cell Indices ◽  
Mean Corpuscular Haemoglobin ◽  
Rbc Count

BACKGROUND Thyroid hormones play an important role in the regulation and production of red blood cells. Thyroid dysfunction induces different effects on blood cells such as anaemia, erythrocytosis, leucopenia, thrombocytopenia and alteration in red cell indices. In this study, we wanted to compare the changes in haematological parameters of thyroid dysfunction patients with those of euthyroid group. METHODS This was a retrospective study done on 310 individuals by collecting data from the medical records. Later the patients were categorized into hypothyroid (33) thyroid stimulating hormone (TSH > 5.5 μIU/mL), hyperthyroid (19) (TSH < 0.3 μIU/mL) and euthyroid (258) (TSH = 0.3 - 5.5 μIU/ml) groups. The haematological parameters of all these patients were obtained by 5-part automated cell count analyser. Finally, the obtained data was analyzed by statistical package for social sciences (SPSS) software. RESULTS The data obtained from the analysis revealed statistically significant (P < 0.05) difference between hypothyroidism, hyperthyroidism and euthyroidism in mean red blood cell (RBC) count, mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), red cell distribution width (RDW), white blood cell (WBC) count and platelet count but the difference was not significant for mean haemoglobin, mean corpuscular haemoglobin concentration (MCHC) (P > 0.05). The mean haemoglobin was lower in hypothyroid patients when compared to euthyroid and hyperthyroid patients. The RBC count (P < 0.007), MCH (P = 0.002) and RDW (P < 0.001) showed statistically significant difference between hypothyroidism and euthyroidism, MCV (P = 0.005) showed statistically significant difference between hyperthyroid and euthyroid groups. CONCLUSIONS In case of patients with abnormal haematological parameters, thyroid hormones evaluation is necessary. KEYWORDS Hypothyroidism, Hyperthyroidism, Haemoglobin, Blood Count, Red Cell Indices

A non-invasive measurement method of pipeline flow rate based on dual FBG sensors

2022 ◽  
pp. 1-1
Author(s):  
Cong Li ◽  
Cong Li ◽  
Ming-Yao Liu ◽  
Han Song ◽  
Xue-Li Yang ◽  
...  
Keyword(s):  
Flow Rate ◽  
Measurement Method ◽  
Non Invasive ◽  
Pipeline Flow ◽  
Fbg Sensors ◽  
Invasive Measurement

METHOD OF ERYTHROCYTE PROTECTION IN URGENT CARDIAC SURGERY

2021 ◽  
Vol 8 (2) ◽  
pp. 68-74
Author(s):  
Volodymyr І. Cherniy ◽  
Lada O. Sobanska
Keyword(s):  
Cardiac Surgery ◽  
Blood Cells ◽  
Blood Count ◽  
Negative Impact ◽  
Hemoglobin Concentration ◽  
Negative Influence ◽  
Control Group ◽  
Extracorporeal Circuit ◽  
Free Hemoglobin ◽  
Erythrocyte Membrane Permeability

Aim: The use of a simple, safe and convenient method of treatment extracorporeal circuit with «adaptation composition » (AdC) for the reduction of negative impact on the state of erythrocytes and tissue hypoxia. Material and methods: The research included 150 patients. They were divided into two groups. The first group included patients who underwent surgery with the treatment of an extracorporeal circuit by AdC, the second group was the control group. Сomplete blood count parameters, plasma free hemoglobin concentration, erythrocyte mechanical and osmotic resistance, erythrocyte membrane permeability and acid hemolysis were studied. Results: Use of AdC prevents adsorption the blood cells on the surface of the extracorporeal circuit and protects the erythrocytes during cardiopulmonary bypass in urgent cardiac surgery. Conclusions: The treatment of oxygenator with AdC reduces the negative influence СРВ on state of RBC. Membranes of erythrocytes were more resistant to traumatic factors in the group with AdC.

scholarly journals Hematology of Wild Caught Dubois’s Tree FrogPolypedates teraiensis, Dubois, 1986 (Anura: Rhacophoridae)

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Madhusmita Das ◽  
Pravati Kumari Mahapatra
Keyword(s):  
Blood Cell ◽  
Blood Serum ◽  
Blood Cells ◽  
Hematological Parameters ◽  
Hemoglobin Concentration ◽  
Mean Corpuscular Hemoglobin ◽  
Reference Ranges ◽  
Corpuscular Hemoglobin ◽  
Differential Leukocyte Count ◽  
Rbc Count

Blood was analyzed from eighty (forty males and forty females) adult individuals ofPolypedates teraiensisto establish reference ranges for its hematological and serum biochemical parameters. The peripheral blood cells were differentiated as erythrocytes, lymphocytes, eosinophils, neutrophils, monocytes, basophils, and thrombocytes, with similar morphology to other anurans. Morphology of blood cells did not vary according to sex. The hematological investigations included morphology and morphometry of erythrocytes, morphometry of leucocytes, packed cell volume (PCV), hemoglobin content (Hb), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), erythrocyte or red blood cell (RBC) count, leukocyte or white blood cell (WBC) count, differential leukocyte count, and neutrophil to lymphocyte (N/L) ratio. Besides, protein, cholesterol, glucose, urea, uric acid, and creatinine content of blood serum were assayed. Hematological parameters that differed significantly between sexes were RBC count, length and breadth of RBC, neutrophil %, N/L ratio, area occupied by basophils, and diameter of large lymphocyte and eosinophils. The level of glucose, urea, and creatinine in blood serum also significantly differed between sexes.

scholarly journals Prevalence of erythrocyte alloimmunization in polytransfused patients

2011 ◽  
Vol 9 (2) ◽  
pp. 173-178 ◽  
Author(s):  
Roberto de Oliveira Cruz ◽  
Mariza Aparecida Mota ◽  
Fabiana Mendes Conti ◽  
Ricardo Antônio d'Almeida Pereira ◽  
Jose Mauro Kutner ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Blood Cells ◽  
High Probability ◽  
Low Frequency ◽  
Albert Einstein ◽  
Red Cell ◽  
Hemolytic Disease ◽  
High Incidence ◽  
Transfusion Reactions

Objective: To determine the incidence and the rate of red blood cell alloimmunization in polytransfused patients. Methods: A polytransfused patient was defined as having received at least 6 units of red cell concentrates during a 3-month period. The records of all patients (n = 12,904) who had received red blood cell units were examined retrospectively by searching the computer database at Hospital Israelita Albert Einstein in São Paulo, Brazil, over a 6-year period, between 2003 and 2009. Results: During this time, 77,049 red cell concentrate transfusions were performed in 12,904 patients. There were 3,044 polytransfused patients, 227 of whom (7.5%) presented with irregular erythrocyte antibodies. The prevalence of alloantibody specificity was: Anti-E>anti-D>anti-K>anti-C>anti-Dia>anti-c>anti-Jka>anti-S in 227 polytransfused patients. We found combinations of alloantibodies in 79 patients (34.8%), and the most common specificities were against the Rh and/or Kell systems. These antibodies show clinical significance, as they can cause delayed hemolytic transfusion reactions and perinatal hemolytic disease. About 20% of the patients showed an IgG autoantibody isolated or combined with alloantibodies. Interestingly, a high incidence of antibodies against low frequency antigens was detected in this study, mainly anti-Dia. Conclusion: Polytransfused patients have a high probability of developing alloantibodies whether alone or combined with autoantibodies and antibodies against low frequency antigens. Transfusion of red blood cells with a phenotype-compatible with RH (C, E, c), K, Fya, and Jka antigens is recommended for polytransfused patients in order to prevent alloimmunization and hemolytic transfusion reactions.

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