Light Scattering and Flow Dichroism Studies on DNA After the Photoreaction with Psoralen

1972 ◽  
Vol 27 (2) ◽  
pp. 196-200 ◽  
Author(s):  
S. Marciani ◽  
M. Terbojevic ◽  
F. Dall’Acqua
Keyword(s):  
Heat Treatment ◽  
Molecular Weight ◽  
Light Scattering ◽  
Double Helix ◽  
Heat Denaturation ◽  
Scattering Measurements ◽  
Helix Structure ◽  
Double Helix Structure

Light scattering measurements performed on DNA after irradiation in the presence of psoralen clearly show that inter strand cross linkings are present in the macromolecule. In fact after heat denaturation and successive cooling irradiated macromolecule shows a molecular weight practically unchanged while a DNA sample after the same treatment shows a molecular weight half of the intact native DNA. Also the general conformation of irradiated DNA undergoes practically to no modifications after the same heat treatment while native DNA shows itself to have been strongly modified. Moreover, on the basis of flow dichroism determinations, DNA cross-linked by psoralen after heat denaturation showed to be able to restore its ordered double helix structure, during the successive cooling.

Über die UV-Bestrahlung von Desoxyribonucleinsäure in Glykol

1965 ◽  
Vol 20 (2) ◽  
pp. 141-143 ◽  
Author(s):  
Hanswerner Dellweg ◽  
Adolf Wacker
Keyword(s):  
Double Helix ◽  
Heat Denaturation ◽  
Thymine Dimer ◽  
Hydration Layer ◽  
Helix Structure ◽  
Double Helix Structure ◽  
Dna Double Helix ◽  
Structure In Solution

Glycol causes a denaturation of the DNA double helix structure in solution. As could be shown earlier, heat denaturation of DNA leads to an increased dimerization of thymine following uvirradiation. In contrast to this, thymine dimer is not increased - but is even slightly decreased - when DNA is uv-irradiated in the presence of glycol. These results are discussed with regard to the distortion of the hydration layer and the hydrophobic stacking of bases, as influenced by glycol.

A STUDY OF A WATER-SOLUBLE COMPLEX OF PRODIGIOSIN PRODUCED BY A STRAIN OF SERRATIA MARCESCENS

1962 ◽  
Vol 40 (1) ◽  
pp. 1019-1024 ◽  
Author(s):  
Seiichi Yoshida
Keyword(s):  
Molecular Weight ◽  
Light Scattering ◽  
Serratia Marcescens ◽  
Average Molecular Weight ◽  
Electrophoretic Analysis ◽  
Water Soluble ◽  
Soluble Complex ◽  
Ph Values ◽  
Scattering Measurements ◽  
Water Soluble Complex

A water-soluble pigment excreted from Serratia marcescens has been purified by precipitation with ammonium sulphate, dialysis, and ultracentrifugation at different pH values. The purified pigment showed a single band in the ultracentrifuge and by electrophoretic analysis at several pH values. An average molecular weight of 5 × 106 was calculated from light-scattering measurements. This pigment is composed of carbohydrate and protein combined with prodigiosin, and several properties of the complex are described.

The Effect of Crystal Packing on Oligonucleotide Double Helix Structure

1987 ◽  
Vol 5 (3) ◽  
pp. 557-579 ◽  
Author(s):  
Richard E. Dickerson ◽  
David S. Goodsell ◽  
Mary L. Kopka ◽  
Philip E. Pjura
Keyword(s):  
Crystal Packing ◽  
Double Helix ◽  
Helix Structure ◽  
Double Helix Structure

A STUDY OF A WATER-SOLUBLE COMPLEX OF PRODIGIOSIN PRODUCED BY A STRAIN OF SERRATIA MARCESCENS

1962 ◽  
Vol 40 (8) ◽  
pp. 1019-1024 ◽  
Author(s):  
Seiichi Yoshida
Keyword(s):  
Molecular Weight ◽  
Light Scattering ◽  
Serratia Marcescens ◽  
Average Molecular Weight ◽  
Electrophoretic Analysis ◽  
Water Soluble ◽  
Soluble Complex ◽  
Ph Values ◽  
Scattering Measurements ◽  
Water Soluble Complex

A water-soluble pigment excreted from Serratia marcescens has been purified by precipitation with ammonium sulphate, dialysis, and ultracentrifugation at different pH values. The purified pigment showed a single band in the ultracentrifuge and by electrophoretic analysis at several pH values. An average molecular weight of 5 × 106 was calculated from light-scattering measurements. This pigment is composed of carbohydrate and protein combined with prodigiosin, and several properties of the complex are described.

DISSOCIATION AND SUBUNIT SIZE OF β-LIPOVITELLIN

1965 ◽  
Vol 43 (6) ◽  
pp. 661-670 ◽  
Author(s):  
W. H. Cook ◽  
R. A. Wallace
Keyword(s):  
Molecular Weight ◽  
Light Scattering ◽  
Dissociation Constants ◽  
Dry Weight ◽  
Ph Values ◽  
Scattering Measurements ◽  
Solvent Displacement ◽  
Weight Method ◽  
Kinetics Of ◽  
Good Agreement

Dissociation of β-lipovitellin was detectable at pH 5.8 and increased linearly with pH. Light-scattering measurements at different pH values were consistent with a molecular weight of 2.27 × 105 for the subunit and twice this value for the associated form, confirming that it is a monomer–dimer system. This value for the molecular weight of the dimer is somewhat higher than previously reported (4.0 × 105) partly because a solvent displacement correction was used in estimating concentration by a dry weight method. Dissociation constants evaluated from light-scattering measurements and by ultracentrifugal separation were 22 × 10−6 and 29 × 10−6 respectively, in good agreement with provisional values already reported. Preliminary studies on the kinetics of this reaction indicate that, when the pH is altered, dissociation reaches the new equilibrium in about 0.5 minute but that reassociation requires about 50 minutes.

Imaging of the DNA (deoxyribonucleic acid) Double Helix Structure by Noncontact Atomic Force Microscopy

1999 ◽  
Vol 38 (Part 2, No. 11A) ◽  
pp. L1211-L1212 ◽  
Author(s):  
Yasushi Maeda ◽  
Takuya Matsumoto ◽  
Hiroyuki Tanaka ◽  
Tomoji Kawai
Keyword(s):  
Atomic Force Microscopy ◽  
Deoxyribonucleic Acid ◽  
Double Helix ◽  
Force Microscopy ◽  
Atomic Force ◽  
Helix Structure ◽  
Double Helix Structure

scholarly journals C3′ Stereocentre of Furanose Determines the Helicity of Double-Stranded Nucleic Acids: Towards Etiology of Nucleic Acids

2020 ◽  
Author(s):  
Anuj Kumar ◽  
Amol Tagad ◽  
G. Naresh Patwari
Keyword(s):  
Molecular Dynamics ◽  
Nucleic Acids ◽  
Structural Transition ◽  
Double Helix ◽  
Helical Structure ◽  
Structural Features ◽  
Toggle Switch ◽  
Helix Structure ◽  
Double Helix Structure ◽  
Dynamics Simulations

ABSTRACTRibose containing double-stranded nucleic acids exhibit helical structure, whereas sugar modified (xeno) nucleic acids may exhibit different structural features. The structural landscape of four stereo variants of furanosal nucleic acids and their C2′ deoxy counterparts, explored with molecular dynamics simulations, suggest that the configuration at the C3′ position plays a pivotal role in determining the helicity. The C3′ stereocentre acts as toggle-switch for the helix to ladder structural transformation by changing the nature of intra-strand interactions resulting in the optimal helices for ribose containing double-stranded nucleic acids. Interestingly, lack of chirality at the C2′ position results in better quality helices than inversion of stereochemistry relative to ribose. The etiology of furanosal-RNA over other furanoses can be hypothesized based on the helical structure, which can effectively be exploited by the biological machinery.SIGNIFICANCEThe double helix structure of furanosal RNA is governed by the configuration at the C3′ position. Furanose sugars such as xylose and lyxose where in the configuration at the C3′ position is inverted relative to the ribose do not form double helix structure, instead result in ladder-like structure. The configuration at the C3′ position acts as a toggle switch for the helix to ladder structural transition. Among four furanose sugars viz., ribose, arabinose, xylose, and lyxose, the double-stranded nucleic acids incorporating ribose form helices with best aspect ratio between major and minor grooves.

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