Flavanes from Dracaena cambodiana

Two new flavanes, (2S)-4′ ,7-dihydroxy-6,8-dimethylflavane (1), and (2S)-5,7-dihydroxy-4′-methoxy- 8-methylflavane (2), together with five known flavanes, (2S)-3′ ,7-dihydroxy-4′-methoxy-8- methylflavane (3), (2R)-4′,7-dihydroxy-8-methylflavane (4), (±)-3′,7-dihydroxy-4′-methoxyflavane (5), (±)-4′,7-dihydroxy-3′-methoxyflavane (6), and (2S)-4′,7-dihydroxyflavane (7), were isolated from the stem of Dracaena cambodiana. Their structures were determined by spectroscopic techniques (UV, IR, 1D and 2D NMR). Their antimicrobial activities were preliminarily examined by the filter paper disc agar diffusion method.

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A New 5-Acyl-2-methylpyrrole from the Endophytic Fungus S20 of Cephalotaxus hainanensis

Natural Product Communications ◽

10.1177/1934578x0900401110 ◽

2009 ◽

Vol 4 (11) ◽

pp. 1934578X0900401 ◽

Cited By ~ 1

Author(s):

Wen-jun Dai ◽

Hao-fu Dai ◽

Jiao Wu ◽

Jian Liu ◽

Wen-li Mei

Keyword(s):

Staphylococcus Aureus ◽

Filter Paper ◽

Endophytic Fungus ◽

2D Nmr ◽

Diffusion Method ◽

Inhibitory Effects ◽

Filter Paper Disc ◽

Spectroscopic Evidence ◽

Agar Diffusion Method ◽

Paper Disc

A new 5-acyl-2-methylpyrrole, isolated from the endophytic fungus S20 of Cephalotaxus hainanensis, was elucidated as 1-(5-methyl-1H-pyrrol-2-yl)-2-((2S*,3R*)-3-((E)-prop-1-enyl)oxiran-2-yl)ethanone (1) on the basis of spectroscopic evidence, including 1D- and 2D-NMR (HMQC, 1H-1H COSY, HMBC and ROESY) and MS analysis. Compound 1 showed inhibitory effects on Staphylococcus aureus and methicillin-resistant S. aureus by the filter paper disc agar diffusion method.

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ASCOCHLORIN, A NEW ANTIBIOTIC, FOUND BY PAPER-DISC AGAR-DIFFUSION METHOD. I

The Journal of Antibiotics ◽

10.7164/antibiotics.21.539 ◽

1968 ◽

Vol 21 (9) ◽

pp. 539-544 ◽

Cited By ~ 79

Author(s):

GAKUZO TAMURA ◽

SEIKICHI SUZUKI ◽

AKIRA TAKATSUKI ◽

KUNIO ANDO ◽

KEI ARIMA

Keyword(s):

Diffusion Method ◽

Agar Diffusion ◽

Agar Diffusion Method ◽

Paper Disc

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Chemical Composition and Antibacterial Activity of Essential Oil from the Basil Cultivated in Heilongjiang

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.183-185.915 ◽

2011 ◽

Vol 183-185 ◽

pp. 915-919 ◽

Cited By ~ 1

Author(s):

Zhan Guo Lu ◽

Lin Wang ◽

Wei Li

Keyword(s):

Escherichia Coli ◽

Chemical Composition ◽

Essential Oil ◽

Antibacterial Activities ◽

Ocimum Basilicum ◽

Diffusion Method ◽

Inhibitory Effects ◽

Filter Paper Disc ◽

Agar Diffusion Method ◽

Paper Disc

The essential oil was obtained from the white Basil (Ocimum basilicum L.) grown in Heilongjiang by hydrodistillation in a clevenger apparatus with 0.28% yield. Then the chemical composition of the essential oil was analyzed by GC-MS. A total of 25 constituents were detected, 24 compounds representing for 98.99% were identified, of which the most abundant compound was β-linalool (64.421%). The antibacterial activities of oil were tested against 3 different general of bacteria and were compared with that of the Penicillin sodium, using the filter paper disc agar diffusion method. The result showed that the oil has powerful inhibitory effects against Staphylicoccus albus, followed by Bacillus subtilis, Escherichia coli.

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Evaluation of Antimicrobial Activities of Crude Methanol Extract of Phoenix dactylifera Seeds on Clinical Isolates of Different Strains of E. coli

International Journal of Biochemistry Research & Review ◽

10.9734/ijbcrr/2019/v25i130066 ◽

2019 ◽

pp. 1-7

Author(s):

C. Eze Chinelo ◽

N. Ezeibe Ezinwanne ◽

A. Ozioko Chizoba ◽

C. Agbo Martina ◽

N. Ugwu Chineye ◽

...

Keyword(s):

Methanol Extract ◽

Phoenix Dactylifera ◽

Antimicrobial Activities ◽

Seed Extract ◽

Diffusion Method ◽

Phytochemical Analysis ◽

E Coli ◽

Agar Diffusion ◽

Agar Diffusion Method ◽

Crude Methanol Extract

Aim: This study was conducted to evaluate the antimicrobial activities of seed extract of Phoenix dactylifera. Study Design: Extraction of active ingredients of Phoenix dactylifera seeds using methanol and its effects on selected clinical isolates and isolated strains of E. coli. Place and Duration of Study: `Department of Phatrmaceutical Microbiology and Biotechnology, Faculty of Pharmaceutical, University of Nigeria, Nsukka between march 2017 and October 2018. Methodology: The seed of phoenix dactylifera were washed thoroughly to get rid of any adhering date flesh, air dried and ground. The ground powders were subjected to extraction by cold maceration using methanol. Preliminary sensitivity test was carried out against eight microorganisms, namely: Pseudomonas aeruginosa, E. coli, Enterococcus, Klebsiella, Staph. spp, Salmonella spp, Bacillus sp and Candida albicans using agar diffusion method. Antimicrobial susceptibility tests were carried out on isolated strains of E. coli from urine samples using agar diffusion method. The minimal inhibitory concentrations of crude methanol extract of seeds of Phoenix dactylifera were determined using agar diffusion method. The phytochemical analysis was conducted to determine the secondary metabolites. Results: The preliminary sensitivity test performed shows that the crude methanol extract of date seed were sensitive against all organisms tested. The active ingredients of the extract showed activities against all strains of E. coli tested though their zones of inhibition vary (18-21mm). The minimum inhibitory concentration (MIC) value ranges from 10-19.9 mg/ml. The phytochemical analysis of crude methanol seed extract of P. dacterifera indicated the presence of alkaloids, flavonoids, tannins, carbohydrates, proteins, reducing sugar, sterols and Terpenes, Anthraquinone glycosides. Conclusion: The present study provides the scientific information about Phoenix dactylifera seed activity.

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Antibacterial Activity of Thujaoccidentalis,ThujaorientalisandChamaecyparisobtusa

International Journal of Pharmaceutical Quality Assurance ◽

10.25258/ijpqa.v8i03.9567 ◽

2017 ◽

Vol 8 (03) ◽

Author(s):

Kyoung- Sun Seo ◽

Seong Woo Jin ◽

Seongkyu Choi ◽

Kyeong Won Yun

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Antibacterial Agent ◽

Methanol Extract ◽

The Other ◽

Diffusion Method ◽

E Coli ◽

Agar Diffusion ◽

Agar Diffusion Method ◽

Crude Methanol Extract

The antibacterial activity of three Cupressaceae plants (Thujaoccidentalis,ThujaorientalisandChamaecyparisobtusa) was tested against three bacteria using the agar diffusion method. The ether and ethylacetate fraction of crude methanol extract from the three plants showed potent antibacterial activity against the tested microorganisms. The result showed that Staphylococcus aureus revealed the most sensitivity among the tested bacteria. Thujaoccidentalisether fraction and Thujaorientalis hexane fraction exhibited the highest antibacterial activity against Staphylococcus aureus. E. coli was shown the highest MIC values compared to the other two tested bacteria, which indicates the lowest antibacterial activity against the bacterium. This study promises an interesting future for designing a potentially active antibacterial agent from the three Cupressaceae plants.

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UJI AKTIVITAS ANTIBAKTERI FORMULASI GEL UNTUK SARIAWAN DARI EKSTRAK DAUN SAGA ( Abrus precatorius Linn. ) TERHADAP BAKTERI Staphylococcus aureus

Jurnal Ilmiah Manuntung ◽

10.51352/jim.v2i2.72 ◽

2017 ◽

Vol 2 (2) ◽

pp. 239

Author(s):

Ratih Dyah Pertiwi ◽

Joni Kristanto ◽

Graha Ayu Praptiwi

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Chemical Constituents ◽

Optimal Concentration ◽

Diffusion Method ◽

Abrus Precatorius ◽

Agar Diffusion ◽

Medicinal Properties ◽

Agar Diffusion Method ◽

Gel Preparation

One of plants used by Indonesian people as a traditional medicine is saga plant (Abrus precatorius L.). This plant has medicinal properties as medication for thrush, cough and laryngitis. Chemical constituents contained in sage leaves which work as antibacterial are flavonoid and saponin. This study aims to determine the optimal concentration of saga leaves extract which can be formulated in a gel dosage form with qualified physical evaluation and has antibacterial activity against Staphylococcus aureus. Saga leaves extract is prepared by maceration method using ethanol 70% as solvent, and then the extract obtained is preliminarily tested to see its antibacterial activity against Staphylococcus aureus with agar diffusion method. Gel formulation for thrush is made with dispersion method in three formulas with variants of active substance concentration that is FI (1%), FII (3%) and FIII (5%). Gel preparation antibacterial activity test is conducted with agar diffusion method as a plate cylinder. Based on this research, it was found that the extract of saga leaves which is positively made is efficacious as antibacterial and can be formulated into a gel preparation for thrush with optimal concentration in F III (5%), this is indicated by widest diameter of the inhibition area against Staphylococus aureus bacteria

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Evaluation of the antimicrobial effect of calcium hydroxide combined to different vehicles

Revista Odonto Ciência ◽

10.15448/1980-6532.2016.2.21522 ◽

2016 ◽

Vol 31 (2) ◽

pp. 89

Author(s):

Tatiana Dantas Piana ◽

Maria de Fátima Malvar Gesteira ◽

Erica Dos Santos Carvalho ◽

Josilene Borges Torres Lima Matos ◽

Monica Franca ◽

...

Keyword(s):

Calcium Hydroxide ◽

Enterococcus Faecalis ◽

Antimicrobial Effect ◽

Antimicrobial Action ◽

Diffusion Method ◽

Agar Diffusion ◽

Inhibition Zones ◽

Agar Diffusion Method

Objective: To evaluate the potentiating effect of different substances in antimicrobial action of calcium hydroxide.Methods: The agar diffusion method, was used with well technique, to analyze seven substances associated to calcium hydroxide to make some pastes, they are: chlorexidine, saline, anesthetic, malvatricin, propolis, hypochlorite, paramonochlorophenol.Results: The pastes with malvatricin and paramonochlorophenol presented greater inhibition zones against Enterococcus faecalis.Conclusion: There was potentialization in antimicrobial effect of calcium hydroxide when associated to paramonochlorophenol and to malvatricin especially against Enterococcus faecalis. Low increase of the antimicrobial capacity was observed when using chlorexidine as vehicle to calcium hydroxine paste.

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