Recovery of the Nucleic Acids of Tobacco Mosaic and Potato X Viruses from Polyacrylamide Gel and Evidence for a Single Infectious Component in Each of the Two Viruses

1973 ◽  
Vol 28 (9-10) ◽  
pp. 618-625 ◽  
Author(s):  
Satyabrata Sarkar
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Tobacco Mosaic Virus ◽  
Ribonucleic Acid ◽  
Gel Electrophoresis ◽  
Glass Tube ◽  
Polyacrylamide Gel ◽  
Biologically Active ◽  
Viral Rnas ◽  
Infectivity Assay

Abstract RNA, tobacco mosaic virus, potato X virus, gel-electrophoresis, infectivity A simple device is standardised for the elution of biologically active ribonucleic acid from polyacrylamide gel after electrophoresis. The pieces of gel containing the nucleic acid to be re­ covered are held in position by two short cylinders of foam rubber in a glass tube and the nucleic acid is concentrated over a sucrose-containing buffer layer by electrophoresis in a standard electro­ phoretic equipment. Using this method nucleic acids can be recovered undamaged almost quanti­ tatively, as shown for microgram quantities of two viral RNAs by infectivity assay. The results offer additional experimental support to the single-component character of tobacco mosaic and potato X viruses.

Properties of the single protein and two nucleic acids of tomato ring-spot virus

1977 ◽  
Vol 55 (8) ◽  
pp. 1028-1037 ◽  
Author(s):  
Wayne R. Allen ◽  
H. F. Dias
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Gel Electrophoresis ◽  
Average Molecular Weight ◽  
Virus Genome ◽  
Protein Subunit ◽  
Spot Virus ◽  
Single Protein ◽  
Middle Component ◽  
Ring Spot

Purified preparations of several isolates of tomato ring-spot virus were shown by rate-zonal centrifugation in sucrose and equilibrium centrifugation in CsCl to be composed of two individual nucleoprotein components. Acrylamide-gel electrophoresis showed that the lighter (middle) component contained a nucleic acid (RNA 2) that was distinct from the species (RNA 1) contained in the heavier (bottom) component. The bottom was more infectious than the middle component and infectivity was enhanced by mixing the components, indicating that the virus genome is divided between component types. Similar results were obtained from infectivity tests on the two nucleic acids. The nucleic acid contents of the middle and bottom components were about 40 and 41%, respectively. The average molecular weights of RNA 2 and RNA 1 from three virus isolates, as determined by acrylamide-gel electrophoresis, were 2.5 and 2.6 × 106, respectively. Molecular complexing between the RNA species during electrophoresis was prevented with the use of formamide. The single protein subunit from the same three isolates had an average molecular weight of about 58 000. Serological comparisons of five tomato ring-spot isolates associated with diseases of fruit trees and grapevines indicated that only the grape yellow vein strain was antigenically distinct. These and other properties indicate that this virus is similar to other members of the nepovirus group.

The influence of proteins on silver staining of nucleic acids following polyacrylamide gel electrophoresis

1995 ◽  
Vol 16 (1) ◽  
pp. 903-904 ◽  
Author(s):  
Friederike Hilbert ◽  
Burkhard Mayr ◽  
Fritz Lackner ◽  
Friedrich Bauer
Keyword(s):  
Nucleic Acids ◽  
Silver Staining ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel

Changes in the nucleic acid content of the uterus of sheep during involution following ovariectomy and following oestrogenic stimulation

1976 ◽  
Vol 27 (5) ◽  
pp. 669 ◽  
Author(s):  
DA Little ◽  
LJ Lambourne
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Ribonucleic Acid ◽  
Acid Content ◽  
Deoxyribonucleic Acid ◽  
Total Content ◽  
Mean Value ◽  
Nucleic Acid Content ◽  
Uterine Tissue ◽  
Rna Concentration

The concentrations of nucleic acids, deoxyribonucleic acid (DNA) and ribonucleic acid (RNA), in ovine uterine tissue were studied to determine whether the ratio RNA/DNA might be used in the bioassay of oestrogenic activity. As the uterus decreased in size with time after ovariectomy, the concentration of DNA increased markedly, but the total content of DNA in the uterus remained constant (mean value 342 ? 8 (SE) mg). The RNA concentration of the tissue decreased during involution, with the result that a highly significant reduction of 33% in the RNA/DNA ratio occurred within 2 weeks of ovariectomy. The ratio decreased further with time. Other ewes were treated for 3 days with diethylstilboestrol dipropionate (10 µg/day), commencing 2 weeks after ovariectomy; the stage of the oestrous cycle at which the ovaries were removed had no effect on the response to the oestrogen. Changes in the nucleic acid concentrations in the uteri of these ewes, and the results obtained during involution, indicate that the RNA/DNA ratio of uterine tissue in ovariectomized ewes has potential as a basis for the bioassay of materials of low oestrogenic potency.

Comparative analysis of the DNA staining efficiencies of different fluorescent dyes in preparative agarose gel electrophoresis

2005 ◽  
Vol 43 (8) ◽  
Author(s):  
Qing Huang ◽  
Wei-Ling Fu
Keyword(s):  
Comparative Analysis ◽  
Nucleic Acid ◽  
Nucleic Acids ◽  
Ethidium Bromide ◽  
Gel Electrophoresis ◽  
Fluorescent Dyes ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Dna Staining

AbstractEthidium bromide (EB) is a mutagen and toxin that is widely used in the laboratory for visualization of nucleic acids. Safer nucleic acid stains, such as SYBR

Can Gold Nanoparticles Aid Electrophoretic Detection of Sulphur in Biomolecules? Development of Gold-PAGE

2018 ◽  
Author(s):  
Emerald R. Tayor ◽  
Silvia Cavuoto ◽  
David M. Beal ◽  
Sophie Caujolle ◽  
Adrian Podoleanu ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Nucleic Acids ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Biological Significance ◽  
Polyacrylamide Gel

<p>The prevalence, distinctive reactivity, and biological significance of sulphur-based groups in proteins and nucleic acids means that analysis of sulphur is of prime importance in biochemistry, biotechnology, and medicine. We report steps in the development of a method to detect these moieties using gold nanoparticles as adjuncts in polyacrylamide gel electrophoresis (Gold-PAGE).<b></b></p>

Glycoproteins, enzymatic activities, and b proteins in intercellular fluid extracts from hypersensitive Nicotiana species infected with tobacco mosaic virus

1985 ◽  
Vol 63 (5) ◽  
pp. 928-931 ◽  
Author(s):  
Jean-Guy Parent ◽  
Richard Hogue ◽  
Alain Asselin
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Electrophoretic Pattern ◽  
Protein Band ◽  
Polyacrylamide Gel ◽  
Intercellular Fluid ◽  
Nicotiana Tabacum L ◽  
The One

Intercellular fluid b proteins from hypersensitive Nicotiana tabacum L. cv. Xanthi-nc and N. sylvestris Speg. and Comes infected with tobacco mosaic virus were compared by two-dimensional (2-D) polyacrylamide gel electrophoresis. Except for missing bands b2, b6a, b6b, and b7b, the overall 2-D electrophoretic pattern of N. sylvestris intercellular fluid proteins was similar to the one observed with 'Xanthi-nc' tobacco. Intercellular proteins were also studied by chromatography on con-canavalin A. Glycoproteins corresponding to b6a and b7a proteins of N. tabacum and the [Formula: see text] analog of N. sylvestris were identified. These proteins are probably peroxidase isozymes, as peroxidase activities with the same electrophoretic mobility were detected after polyacrylamide gel electrophoresis. No esterase activity was associated with any b protein band in gels. Esterase activities decreased upon virus infection, but accumulation of b proteins and peroxidase activities increased.

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