Density-Dependent Growth Inhibiting Interactions between 3T3 and SV40-3T3 Cells in Mixed Cultures

1979 ◽  
Vol 34 (3-4) ◽  
pp. 272-278 ◽  
Author(s):  
Jürgen van der Bosch ◽  
Heinz Maier
Keyword(s):  
High Serum ◽  
Dependent Manner ◽  
3T3 Cells ◽  
Density Dependent ◽  
Inhibition Of Growth ◽  
High Serum Concentration ◽  
Dependent Inhibition ◽  
Cell Population Growth ◽  
Dependent Growth ◽  
Growth Inhibiting

Abstract 3T3 cells are shown to reduce SV40-3T3 cell population growth in a density-dependent manner in co-cultures of 3T3 and SV40-3T3 cells. The development of this inhibitory activity roughly parallels the development of density-dependent inhibition of growth in homogeneous 3T3 control cultures. The extent of reduction of SV40-3T3 growth can be manipulated by pretreatment of 3T3 cells with a high serum concentration. SV40-3T3 growth rates are reduced by factors between 10 and 20 under optimum inhibitory conditions as compared to SV40-3T3 growth in control cultures.

scholarly journals NAD levels in 3T3 cells during exponential growth and density-dependent inhibition of growth

FEBS Letters ◽  
1974 ◽  
Vol 47 (1) ◽  
pp. 23-25 ◽  
Author(s):  
Elaine L. Jacobson ◽  
Myron K. Jacobson ◽  
Carl Bernofsky
Keyword(s):  
Exponential Growth ◽  
3T3 Cells ◽  
Density Dependent ◽  
Inhibition Of Growth ◽  
Dependent Inhibition

Density-Dependent Growth Adaptation Kinetics in 3T 3 Cell Populations Following Sudden [Ca2+] and Temperature Changes. A Comparison with SV40-3T3 Cells

1979 ◽  
Vol 34 (3-4) ◽  
pp. 279-283 ◽  
Author(s):  
Jürgen van der Bosch ◽  
Ilse Sommer ◽  
Heinz Maier ◽  
Willy Rahmig
Keyword(s):  
Growth Rate ◽  
Cell Density ◽  
Cell Number ◽  
Cell Populations ◽  
3T3 Cells ◽  
Density Dependent ◽  
Cell Densities ◽  
Dependent Growth ◽  
Growth Adaptation ◽  
Stationary Cell

Abstract Lowered extracellular [Ca2+] causes low growth rates and low stationary cell densities in 3T3 cell cultures as compared to physiological [Ca2+]. Under otherwise constant conditions the extra­ cellular [Ca2+] determines a stable stationary cell density, which can be readied by increase of net cell number or decrease of net cell number, depending on cell density at the time of [Ca2+] adjustment. SV40-3T3 cells do not show this [Ca2+] dependency. At 39 °C 3T3 and SV40-3T3 cell populations show an increased growth rate at low cell densities as compared to cell populations at 35 °C. Approaching the stationary density the growth rate of both cell sorts is reduced faster at 39 °C than at 35 °C, leading to lower stationary cell densities at 39 °C than at 35 °C. A temperature change from 39 °C to 35 °C or in the opposite direction can affect the stationary cell density of 3T3 cell populations only if applied before reduction of growth rate by density-dependent growth-inhibiting principles has taken place.

Early events during substrate adhesion of normal and virus-transformed mouse fibroblasts

1976 ◽  
Vol 20 (3) ◽  
pp. 479-495
Author(s):  
T.B. Mapstone ◽  
L.A. Culp
Keyword(s):  
Cell Attachment ◽  
Metabolic Inhibitors ◽  
3T3 Cells ◽  
Plastic Substrates ◽  
Substrate Adhesion ◽  
Inhibition Of Growth ◽  
Dependent Inhibition ◽  
The Relationship ◽  
Attachment Level ◽  
Attachment Process

The relationship between attachment of Balb/c3T3 cells and their SV40 transformants to glass or plastic substrates and deposition of substrate-attached material (SAM-proteoglycans implicated in substrate adhesion) has been examined very early after inoculation of cells subcultured with ethylenebis (oxyethylenenitrilo) tetra-acetic acid (EGTA). The metabolic inhibitors cycloheximide and colchicine minimally affected the kinetics or short-term stability of attachment of cells or deposition of SAM. SAM deposition on to the substrate began immediately after inoculation of cells and was maximal prior to the highest cell attachment level (30-40 min after inoculation). At 4 degrees C, there was no attachment of cells to the substrate and no deposition of leucine- or glucosamine-radiolabelled SAM on to the substrate. 3T3 cells deposited SAM to a maximal level earlier during the attachment process than SV40-transformed cells. SVT2 cells deposited much smaller amounts of SAM (measured on a per-cell basis) to 3T3 SAM-coated substrates during attachment processes, whereas 3T3 cells and concanavalin A (con A) revertant variants of SVT2 cells, which have regained density-dependent inhibition of growth, deposited identical amounts of SAM (per-cell) on untreated or SAM-coated substrates. Serial attachment experiments with SVT2 cells indicated that all SVT2 cells reduced their deposition amounts on SAM-coated substrates, rather than there being an ability of a small proportion of cells to attach preferentially to SAM-coated substrates while being unable to deposit SAM themselves. The data are consistent with the presence of a sizeable pool of SAM-like proteoglycans being present on the surface of EGTA-removed cells whose deposition may be a requirement for, but may not necessarily be sufficient for, stable adhesion of cells to the substrate.

Release from Density-Dependent Inhibition of Growth in the Absence of Cell Locomotion

1974 ◽  
Vol 16 (1) ◽  
pp. 181-188
Author(s):  
MARGARET M. YARNELL ◽  
H. P. SCHNEBLI
Keyword(s):  
Insulin Treatment ◽  
Density Dependent ◽  
Mouse Fibroblasts ◽  
Cell Locomotion ◽  
Inhibition Of Growth ◽  
Dependent Inhibition ◽  
Cellular Locomotion

3T3 mouse fibroblasts are released from density-dependent inhibition of growth by treatment with insulin. The same insulin treatment stimulates cell locomotion several hours before any new mitoses become visible. Inhibition of cell locomotion by colcemid does not affect the overgrowth stimulation due to insulin. From this it is concluded that cellular locomotion is not a prerequisite for the release from density-dependent inhibition of growth.

Growth in vitro of tumour cell × fibroblast hybrids in which malignancy is suppressed

1977 ◽  
Vol 25 (1) ◽  
pp. 73-86
Author(s):  
D.S. Straus ◽  
J. Jonasson ◽  
H. Harris
Keyword(s):  
Tumour Cell ◽  
Cloning Efficiency ◽  
Density Dependent ◽  
Inhibition Of Growth ◽  
Dependent Inhibition ◽  
Soft Agarose

We have studied the growth in vitro of a lymphoma × fibroblast hybrid and several melanoma × fibroblast hybrids in which malignancy is suppressed. The parental cells, the hybrids, and malignant segregants derived from the hybrids were analysed for serum requirement, cloning efficiency in soft agarose, density-dependent inhibition of growth, and secretion of plasminogen-activating enzyme. One malignant segregant from the lymphoma × fibroblast cross was found by a number of criteria to have a more highly ‘transformed’ phenotype than the hybrid from which it was derived. However, in the case of the melanoma × fibroblast crosses, none of the parameters examined could be correlated in a direct way with malignancy.

scholarly journals Inhibition of DNA synthesis in SV3T3 cultures by isolated 3T3 plasma membranes.

1983 ◽  
Vol 97 (1) ◽  
pp. 276-279 ◽  
Author(s):  
S W Peterson ◽  
V Lerch
Keyword(s):  
Plasma Membrane ◽  
Dna Synthesis ◽  
Cell Cultures ◽  
Plasma Membranes ◽  
Inhibitory Effect ◽  
Density Dependent ◽  
Inhibition Of Growth ◽  
Dependent Inhibition ◽  
Membrane Components ◽  
Inhibition Of Dna Synthesis

3T3 plasma membranes were added to subconfluent cultures of SV3T3 cells in the presence of fusogens. If this protocol results in the introduction into the SV3T3 cell membrane of 3T3 plasma membrane components responsible for density-dependent inhibition of growth, then the SV3T3 cell cultures would be expected to show decreased rates of DNA synthesis as they approach confluence. Results of these experiments indicate that rates of DNA synthesis in SV3T3 cultures so treated were as much as 63% less than in untreated controls. This effect could not be attributed to the fusogens or to the 3T3 plasma membranes alone. This growth-inhibitory effect is specific for 3T3 membranes and is not observed when SV3T3 plasma membranes are fused with SV3T3 cell cultures. These data support the hypothesis that one aspect of the loss of density-dependent inhibition of growth in SV3T3 cells is a deletion or alteration in plasma membrane components and, further, that density-dependent inhibition of growth can be in part restored to SV3T3 cell cultures by fusing the cells with 3T3 plasma membranes.

scholarly journals Role of serum components in density-dependent inhibition of growth of cells in culture. Platelet-derived growth factor is the major serum determinant of saturation density

1980 ◽  
Vol 85 (2) ◽  
pp. 377-385 ◽  
Author(s):  
A Vogel ◽  
R Ross ◽  
E Raines
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Blood Serum ◽  
Cell Density ◽  
Whole Blood ◽  
Saturation Density ◽  
Platelet Derived Growth Factor ◽  
3T3 Cells ◽  
Density Dependent ◽  
Dependent Inhibition

The effects of platelet-derived growth factor and plasma components on saturation density in cultures of 3T3 cells were investigated. Both of these components of whole blood serum affect saturation density; however, when 3T3 cells become quiescent at high density in medium containing whole blood serum, only platelet-derived growth factor and fresh whole blood serum are capable of stimulating proliferation. Addition of fresh plasma- derived serum has little effect on cell growth. These results suggest that the platelet factor is the major determinant of saturation density in cultures of 3T3 cells maintained in medium supplemented with whole blood serum. Experiments were performed to investigate the mechanism by which platelet-derived growth factor regulates saturation density. We investigated the possibilities of inactivation of growth factors by proliferating cells, and the effects of cell density on the response of 3T3 cells to platelet-derived growth factor. The amount of platelet- derived growth factor required to initiated DNA synthesis increases with increasing cell density. Some inactivation of growth factors by growing cells was detected, but this depletion was only evident at high cell density. We propose that density-dependent inhibition in cultured 3T3 cells is the result both of an increased requirement for the platelet- derived growth factor as the cultures become more crowded and of inactivation of growth factor activity by growing cells.

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