Aloe Plants Accumulate Anthrone-Type Anthranoids in Inflorescence and Leaves, and Tetrahydroanthracenes in Roots

1994 ◽  
Vol 49 (5-6) ◽  
pp. 286-292 ◽  
Author(s):  
Anette Sigler ◽  
Hans W. Rauwald
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Plant Material ◽  
High Performance ◽  
The Other ◽  
Secondary Products ◽  
Inflorescence Axis ◽  
The One ◽  
The Individual ◽  
First Time

Abstract The accumulation and distribution of characteristic secondary products in the different organs of an Aloe plant (A. succotrina Lam.) were studied by high performance liquid chromatography for the first time. In the leaves of the Aloe plant, only anthrone-C-glycosyls of the 7-hydroxyaloin type and, for the first time in plant material, the free anthraquinone 7-hydroxyaloeemodin were found. In contrast to previous reports on the distribution of secondary products in Aloe plants, anthrone-C-glycosyls were also detected in flowers, bracts and the inflorescence axis of the species examined. Aloesaponol I, a tetrahydroanthracene aglycone, was only present in the underground organs and in the stem. The 2-alkylchromone-C-glucosyl aloeresin B showed no specific occurrence as it was found in every type of organ. Based on these results and the findings of recent studies on Aloe roots and flowers, a distri­bution scheme of polyketide types in the Aloe plant was established. It suggests a separate and independent anthranoid metabolism for underground Aloe organs and stem on the one hand, and for leaves and inflorescence organs on the other hand. In the latter structures anthranoid metabolism seems to be additionally compartmentalized as the anthranoid pro­ files of inflorescence organs and leaves differ in two points relevant to anthranoid biosynthe­ sis: firstly, the occurrence of anthrone aglycones and secondly, the individual content of corre­sponding anthrone-C-glucosyl diastereomers.

scholarly journals Identificatin and quantiative analysis of amyrins in Humulus lupulus L.

2014 ◽  
Vol 27 (3) ◽  
pp. 142-144 ◽  
Author(s):  
Kamila Rokicka ◽  
Magdalena Wojciak-Kosior
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Plant Material ◽  
Mobile Phase ◽  
High Performance ◽  
Humulus Lupulus ◽  
The Other ◽  
Humulus Lupulus L ◽  
Validation Parameters ◽  
Quantiative Analysis

ABSTRACT In the paper, the identification and quantification of α- and β-amyrin in five varieties of Humulus lupulus is described. The plant samples were extracted with ethyl acetate and analyzed using of high performance liquid chromatography (HPLC) with PAD detection. The separation was achieved on RP 18 column, at 2 mL/min flow rate and at temperature of 35°C. Acetonitrile was used as a mobile phase. The established calibration curves and the other validation parameters: linearity (correlation coefficient r > 0.9988) and precision (RSD values ranged from 0.14 to 1.81%) were found to be satisfactory for the proposed method. The content of α- and β-amyrin strongly depended on varieties of H. lupulus and amounted from 72.7 to 232.5 μg/g and from 77.9 to 176.9 μg/g of dry plant material, respectively.

scholarly journals Flavonoids From the Flowers and Leaves of the Himalayan Megacodon stylophorus (Gentianaceae)

2021 ◽  
Vol 16 (2) ◽  
pp. 1934578X2199226
Author(s):  
Tsukasa Iwashina ◽  
Rinchen Yangzom ◽  
Hari Prasad Devkota ◽  
Takayuki Mizuno
Keyword(s):  
Mass Spectrometry ◽  
Nuclear Magnetic Resonance ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
The Other ◽  
Liquid Chromatography Mass Spectrometry ◽  
The Family ◽  
First Time ◽  
Layer Chromatography

Five flavonol O-glycosides and 4 C-glycosylflavones were isolated from the flowers of the Himalayan Megacodon stylophorus (Gentianaceae). They were characterized as quercetin 3- O-glucoside (1), quercetin 3- O-rutinoside (2), kaempferol 3- O-glucoside (3), isorhamnetin 3- O-glucoside (4) and kaempferol 3,7-di- O-glucoside (5) (flavonols), and isovitexin (6), isoorientin (7), isovitexin X″- O-arabinoside (8) and isovitexin 4′- O-glucoside (9) ( C-glycosylflavones) by ultraviolet, liquid chromatography-mass spectrometry, acid hydrolysis, nuclear magnetic resonance, and/or high-performance liquid chromatography and thin-layer chromatography comparisons with authentic samples. On the other hand, 5 C-glycosylflavones were isolated from the leaves and identified as 6, 7, 9, vitexin (10), and orientin (11). Although many C-glycosylflavones and xanthones have been reported from Gentianaceae species, flavonols are minor occurrence in the family. Flavonoids were reported from the Megacodon species for the first time.

Compositional Analysis of Organosolv Poplar Lignin by Using High-Performance Liquid Chromatography/High-resolution Multi-stage Tandem Mass Spectrometry

2021 ◽  
Author(s):  
Jifa Zhang ◽  
Yuan Jiang ◽  
Leah F Easterling ◽  
Anton Anster ◽  
Wanru Li ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Compositional Analysis ◽  
Complex Mixtures ◽  
Tandem Mass ◽  
Multi Stage ◽  
The Individual ◽  
Environmentally Friendly Process

Organosolv treatment is an efficient and environmentally friendly process to degrade lignin into small compounds. The capability of characterizing the individual compounds in the complex mixtures formed upon organosolv treatment...

scholarly journals EFFECT OF USING L-ASPARAGINASE IN REDUCING ACRYLAMIDE PERCENTAGE IN BISICUT

2016 ◽  
Vol 47 (4) ◽  
Author(s):  
Jebur & et al.
Keyword(s):  
High Performance Liquid Chromatography ◽  
Enzyme Activity ◽  
Liquid Chromatography ◽  
High Performance ◽  
Specific Activity ◽  
Control Sample ◽  
The Other ◽  
Enzyme Efficiency ◽  
Negative Effect ◽  
The Stability

This study was aimed to know the efficiency of partially purified L- asparaginase produced from local isolate from Erwinia spp. to reduce the percentage of acrylamide formed in Biscuit. Four types of biscuit from wheat flour were prepared (T1, T2, T3, T4),and T1 as control. High performance liquid chromatography technique was used to estimate acrylamide ratio in biscuit , Effect of enzyme addition  on flour chemical and rheological properties was studied, also dough behavior ,gluten percentage, water absorption and amylase enzyme activity was estimated. The results revealed  that  the  addition of  experimental asparaginase ( specific activity 20.5 unite mg-1 ) with 1% of flour weight lead to reduce in acrylamide formation in Biscuit  to 89 %  compared  to  control sample ( in absence of enzyme ) . Moreover, the addition of Asparagine to flour at 0.1 % of its weight, where L- asparaginase was available caused a negative effect on enzyme efficiency in reducing the acrylamide in biscuit. So the level of acrylamide was reduced to 57.7 %. In the other hand , the percentage of acryl amide in biscuit was increased to   233 % when the asparagine was added to mixture in absence of L- asparaginase .Addition of  the enzyme to flour have no effect on the percentage value of gluten but improved the  stability of dough .The  enzyme  addition also led to increase amylases activities.  Addition of experimental enzyme had no effect on quality and sensory evaluation of biscuit.

ANTHOCYANIN, AGLYCONE AND AGLYCONE-SUGAR CONTENT OF FRUITS OF TEMPERATE NORTH AMERICAN SPECIES OF Gaylussacia (ERICACEAE)

1988 ◽  
Vol 68 (1) ◽  
pp. 247-253 ◽  
Author(s):  
J. R. BALLINGTON ◽  
W. E. BALLINGER ◽  
E. P. MANESS
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
North American ◽  
High Performance ◽  
Hplc Analysis ◽  
Sugar Content ◽  
North American Species ◽  
The Other ◽  
Phylogenetic Implications

HPLC analysis of the true huckleberry species Gaylussacia baccata, G. dumosa, G. frondosa, G. mosieri, and G. ursina identified the 3-monoarabinosides, 3-monogalactosides, and 3-monoglucosides of cyanidin, delphinidin, malvidin, peonidin, and petunidin. Gaylussacia brachycera contained all anthocyanins, except peonidin-3-arabinoside. Gaylussacia brachycera differed from other species in percent delphinidin-3-arabinoside. It was higher than the other species in percent of the aglycone delphinidin and lower in cyanidin, and also higher in percent of the sugar arabinose. There were no detectable differences among the other species for anthocyanins, aglycones, or aglycone-sugars. The phylogenetic implications of the similarities among species of Gaylussacia and Vaccinium in anthocyanins, aglycones, and aglycone-sugars of the fruit were discussed.Key words: High-performance liquid chromatography, huckleberries, blueberries, chemotaxonomy, taxonomy, biosystematics

Isolation and identification of tuliposides D and F from tulip cultivars

2020 ◽  
Vol 75 (1-2) ◽  
pp. 7-12 ◽  
Author(s):  
Taiji Nomura ◽  
Shinjiro Ogita ◽  
Yasuo Kato
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Secondary Metabolites ◽  
High Performance ◽  
The Other ◽  
Isolation And Identification ◽  
Tulipa Gesneriana ◽  
Tissue Extracts ◽  
Spectroscopic Analyses ◽  
Hplc Peak

Abstract6-Tuliposides A (6-PosA) and B (6-PosB) are major defensive secondary metabolites in tulip cultivars (Tulipa gesneriana), having an acyl group at the C-6 position of d-glucose. Although some wild tulip species produce 1,6-diacyl-glucose type of Pos (PosD and PosF), as well as 6-PosA/B, they have not yet been isolated from tulip cultivars. Here, aiming at verifying the presence of PosD and PosF in tulip cultivars, tissue extracts of 25 cultivars were analyzed by high-performance liquid chromatography (HPLC). Although no HPLC peaks for PosD nor PosF were detected in most cultivars, we found two cultivars giving a minute HPLC peak for PosD and the other two cultivars giving that for PosF. PosD and PosF were then purified from petals of cultivar ‘Orca’ and from pistils of cultivar ‘Murasakizuisho’, respectively, and their identities were verified by spectroscopic analyses. This is the first report that substantiates the presence of 1,6-diacyl-glucose type of Pos in tulip cultivars.

High-performance liquid chromatography associated with resonance Rayleigh scattering spectral study of the interactions of palladium(ii)–antimigraine chelates with Erythrosin B and their analytical applications

2020 ◽  
Vol 44 (7) ◽  
pp. 3078-3086
Author(s):  
Shiyu Li ◽  
Zhongying Zhang ◽  
Jingdong Peng ◽  
Xiang Wang ◽  
Dengying Long ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Rayleigh Scattering ◽  
Resonance Rayleigh Scattering ◽  
Selective Detection ◽  
Detection Technology ◽  
Highly Sensitive ◽  
Scattering Spectra ◽  
First Time

For the first time, a highly sensitive and selective detection technology of high-performance liquid chromatography associated with resonance Rayleigh scattering spectra (HPLC-RRS) is applied to analyze migraine drugs including zolmitriptan (ZON) and rizatriptan (RIN).

scholarly journals LEXIS AND PHRASEOLOGY, VERBALIZING CONCEPTS OF WOMAN AND GIRL IN A.B. KAITUKOV’S WORKS

2020 ◽  
Author(s):  
Р.Г. ЦОПАНОВА
Keyword(s):  
Mental Content ◽  
Literary Text ◽  
The Other ◽  
Contextual Analysis ◽  
The People ◽  
The World ◽  
The One ◽  
The Individual ◽  
Linguistic Units ◽  
First Time

Целью данного исследования является определение ментального содержания лексики и фразеологии, вербализующей концепты женщина (сылгоймаг) и девушка (чызг) в произведениях осетинского писателя А.Б. Кайтукова. Научная новизна связана с тем, что впервые на языковом материале произведений А. Кайтукова выявлено ментальное содержание указанных концептов. Актуальность данного исследования в том, что, благодаря описанию языкового содержания концептов женщина (сылгоймаг) и девушка (чызг), читатель, с одной стороны, вводится в мир национальной лингвокультуры, содержащей информацию о менталитете народа, с другой стороны – дается характеристика индивидуальных особенностей языка писателя. В работе использованы следующие методы исследования: семантико-стилистический, методы концептуального и контекстуального анализа языковых единиц в художественном тексте. Поставлены следующие задачи: определить номинативную плотность концептов женщина и девушка; раскрыть ментальное содержание лексики и фразеологии, вербализующей названные концепты; указать когнитивные признаки исследуемых концептов; охарактеризовать лексику и фразеологию, объективирующие названные концепты как средство создания идиостиля писателя. В результате работы дана характеристика концептов женщина и девушка в произведениях А. Кайтукова в аспекте лингвокультуры и в рамках идиостиля писателя. The purpose of this study is to determine the mental contents of the vocabulary and phraseology that verbalize the concepts of woman (sylgoymag) and girl (chyzg) in the works of the Ossetian writer A. B. Kaitukov. The scientific novelty is connected with the fact that for the first time the mental content of these concepts will be revealed on the language material of A. Kaitukov's works. The relevance of this study is that due to the description of the linguistic content of the concepts woman (sylgoimag) and girl (chyzg), the reader, on the one hand, is introduced into the world of national linguoculture, containing information about the mentality of the people, on the other hand, a characteristic of the individual features of the writer’s language is given. The following research methods were used in the work: semantic and stylistic, methods of conceptual and contextual analysis of linguistic units in a literary text. The following tasks were set: to determine the nominative density of the concepts woman and girl; to reveal the mental content of lexis and phraseology, verbalizing the named concepts; indicate the cognitive features of the studied concepts; to characterize the vocabulary and phraseology that objectify the named concepts as a means of creating the idiostyle of the writer. As a result of the work, a description of the concepts of a woman and a girl in the works of A. Kaitukov is given in the aspect of linguoculture and within the framework of the writer's idiostyle.

Rapid determination and conversion study of 5-hydroxymethylfurfural and its derivatives in glucose injection

2018 ◽  
Vol 42 (21) ◽  
pp. 17725-17731 ◽  
Author(s):  
Cheng Wang ◽  
Yajing Hou ◽  
Yuanyuan Lin ◽  
Yitong Xie ◽  
Di Wei ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Rapid Determination ◽  
Normal Phase ◽  
Glucose Injection ◽  
First Time ◽  
Conversion Study

5-HMF and two identified additional derivatives (II, III) in glucose injection were rapidly separated in less than 7 min for the first time by our normal phase high performance liquid chromatography. The results were more precise than the method adopted by pharmacopeias.

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