Interaction of Organophosphorus Insecticide Methylparathion with Calf Thymus DNA and a Synthetic DNA Duplex

1995 ◽  
Vol 50 (11-12) ◽  
pp. 820-823 ◽  
Author(s):  
Janusz Blasiak ◽  
Vladimír Kleinwächter ◽  
Zofia Walter ◽  
Renata Žaludová
Keyword(s):  
Cd Spectroscopy ◽  
Organophosphorus Insecticide ◽  
Calf Thymus Dna ◽  
Molar Ratio ◽  
Cd Spectra ◽  
Transition Range ◽  
Synthetic Dna ◽  
Negative Band ◽  
Calf Thymus ◽  
Ct Dna

Abstract The interaction of an organophosphorus insecticide methylparathion (O.O-dimethyl 0-4-nitrophenyl phosphorothioate) with double-stranded DNA was characterized by UV and circular dichroism (CD) spectroscopy. Two kinds of DNA were employed: calf thymus DNA (CT DNA) and a synthetic two-stranded oligomer of sequence 5′-d(TTGGATCCGAATT-CAAGCTT)-3′ Melting curves and CD spectra were taken for the DNAs in the presence of the insecticide at methylparathion/DNA base pair molar ratio of 0.5. The insecticide evoked a decrease of the melting temperature and a broadening of the transition range for CT DNA. Similar effects were observed for the synthetic oligomer but they were less pronounced than in the case of CT DNA. Methylparathion evoked a slight shift and an increase in the amplitude of the negative band in the CD spectra of both DNAs. Obtained results indicate that methylparathion may perturb the thermal stability and conformation of DNA, which is an evidence that the insecticide has an ability to interact directly with DNA.

Cu(ii)-induced twisting of the biphenyl core: exploring the effect of structure and coordination environment of biphenyl-based chiral copper(ii) complexes on interaction with calf-thymus DNA

2020 ◽  
Vol 44 (46) ◽  
pp. 20275-20284
Author(s):  
Soumen Ghosh ◽  
Mehebub Ali Khan ◽  
Arghyadeep Bhattacharyya ◽  
Md. Akhtarul Alam ◽  
Ennio Zangrando ◽  
...  
Keyword(s):  
Optically Active ◽  
Calf Thymus Dna ◽  
Coordination Environment ◽  
Calf Thymus ◽  
Ct Dna ◽  
Effect Of Structure

Biphenyl core-based clip-like receptors get twisted after complexation with Cu2+. The extent of interaction of the optically active complexes with ct-DNA varies depending on the structure and coordination environment.

Interaction of a synthesized pyrene based fluorescent probe with CT-DNA: spectroscopic, thermodynamic and molecular modeling studies

RSC Advances ◽  
2016 ◽  
Vol 6 (96) ◽  
pp. 93335-93342 ◽  
Author(s):  
Soumen Ghosh ◽  
Abdulla Al Masum ◽  
Aniruddha Ganguly ◽  
Md. Akhtarul Alam ◽  
Md. Maidul Islam ◽  
...  
Keyword(s):  
Molecular Modeling ◽  
Fluorescent Probe ◽  
Water Soluble ◽  
Calf Thymus Dna ◽  
Modeling Studies ◽  
Calf Thymus ◽  
Molecular Modeling Studies ◽  
Ct Dna

The present study demonstrates the synthesis of a new pyrene based water soluble fluorescent probe and its interaction with Calf-thymus DNA.

scholarly journals Affinity studies of hypocrellin B and mono-cysteine substituted hypocrellin B with CT-DNA using spectroscopic methods

2005 ◽  
Vol 19 (5,6) ◽  
pp. 259-266 ◽  
Author(s):  
Shaohua Wei ◽  
Jiahong Zhou ◽  
Yuying Feng ◽  
Deyin Huang ◽  
Xuesong Wang ◽  
...  
Keyword(s):  
Deoxyribonucleic Acid ◽  
Double Helix ◽  
Cd Spectroscopy ◽  
Base Stacking ◽  
Calf Thymus ◽  
Visible Spectra ◽  
Hypocrellin B ◽  
Uv Visible ◽  
Dna Double Helix ◽  
Ct Dna

The interaction of anticancer drug hypocrellin B (HB) and mono-cysteine substituted hypocrellin B (MCHB) with calf thymus deoxyribonucleic acid (CT-DNA) has been investigated using spectral methods. The results of UV–visible spectra showed that the HB and MCHB can intercalate into the base-stacking domain of the CT-DNA double helix. Further studies based on fluorescence spectroscopy and circular dichroism (CD) spectroscopy also supported the intercalation mechanism.

scholarly journals CD investigations on conformation of H-X-(Pro)n-Y-OH peptides (X = Trp, Tyr; Y = Tyr, Met); models for intramolecular long range electron transfer.

1995 ◽  
Vol 42 (2) ◽  
pp. 259-268 ◽  
Author(s):  
K L Wierzchowski ◽  
K Majcher ◽  
J Poznański
Keyword(s):  
Electron Transfer ◽  
Long Range ◽  
Peptide Bond ◽  
Cd Spectroscopy ◽  
Helical Conformation ◽  
Large Temperature ◽  
Cd Spectra ◽  
Negative Band ◽  
Left Handed ◽  
In Trans

Conformations of three series of peptides: H-Trp-(Pro)n-Tyr-OH (n = 1-5), H-Trp-(Pro)n-Met-OH (n = 1-3) and H-Tyr-(Pro)n-Met-OH (n = 1-3), used as models in studies on long range electron transfer through protein matrix, were investigated by CD spectroscopy in aqueous solution at pH 5.2 in the temperature range of 10 degrees C-90 degrees C. CD spectra of their component N- and C-terminal dipeptide and oligoproline fragments were also measured under similar conditions. In interpretation of the spectra the cistrans equilibrium about X-Pro bonds was taken into account and CD spectra of Trp-Pro and Tyr-Pro chromophores in trans and cis configuration of the peptide bond were evaluated. The spectra of n = 3-5 peptides from the first series and those with n = 2-3 from the other two series exhibit a strong negative band in the 202-207 nm region, the strength of which is proportional to the number of Pro residues in the (Pro)n bridge, and characterized by a large temperature decrement. In view of close similarity between characteristics of this band and the 206 nm band of aqueous oligoproline peptides (n > or = 3), known to attain a left handed helical conformation similar to that of 3(1) helix of the all-trans poly-L-proline II, this band was attributed to a conformation of the latter type. H-Trp-(Pro)2-Tyr-OH does not form this conformation due to sterical interaction between the two bulky aromatic side chains. Conclusions drawn from analysis of the CD spectra are supported by 1H and 13CNMR data reported elsewhere.

scholarly journals ct-DNA Binding and Antibacterial Activity of Octahedral Titanium (IV) Heteroleptic (Benzoylacetone and Hydroxamic Acids) Complexes

2016 ◽  
Vol 2016 ◽  
pp. 1-11
Author(s):  
Raj Kaushal ◽  
Sheetal Thakur ◽  
Kiran Nehra
Keyword(s):  
Antibacterial Properties ◽  
Antibacterial Activities ◽  
Spectroscopic Technique ◽  
Calf Thymus Dna ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Intercalative Mode ◽  
Heteroleptic Complexes ◽  
Calf Thymus ◽  
Ct Dna

Five structurally related titanium (IV) heteroleptic complexes, [TiCl2(bzac)(L1–4)] and [TiCl3(bzac)(HL5)]; bzac = benzoylacetonate; L1–5 = benzohydroximate (L1), salicylhydroximate (L2), acetohydroximate (L3), hydroxyurea (L4), and N-benzoyl-N-phenyl hydroxylamine (L5), were used for the assessment of their antibacterial activities against ten pathogenic bacterial strains. The titanium (IV) complexes (1–5) demonstrated significant level of antibacterial properties as measured using agar well diffusion method. UV-Vis absorption spectroscopic technique was applied, to get a better insight into the nature of binding between titanium (IV) complexes with calf thymus DNA (ct-DNA). On the basis of the results of UV-Vis absorption spectroscopy, the interaction between ct-DNA and the titanium (IV) complexes is likely to occur through the same mode. Results indicated that titanium (IV) complex can bind to calf thymus DNA (ct-DNA) via an intercalative mode. The intrinsic binding constant (Kb) was calculated by absorption spectra by using Benesi-Hildebrand equation. Further, Gibbs free energy was also calculated for all the complexes.

scholarly journals Perimidine based selective colorimetric and fluorescent turn-off chemosensor of aqueous Cu2+: studies on its antioxidant property along with its interaction with calf thymus-DNA

RSC Advances ◽  
2017 ◽  
Vol 7 (64) ◽  
pp. 40563-40570 ◽  
Author(s):  
Debashis Roy ◽  
Arijit Chakraborty ◽  
Rina Ghosh
Keyword(s):  
Ascorbic Acid ◽  
Antioxidant Property ◽  
Calf Thymus Dna ◽  
Calf Thymus ◽  
Ct Dna

A perimidine derivative 1 selectively senses nanomolar aqueous Cu2+ spectrophotometrically, exhibits better antioxidant capability than l-ascorbic acid, and interacts with CT-DNA.

Thermodynamic investigation of manganese(III) 5-(1-(4-carboxybutyl)pyridinium-4-yl) 10,15,20-tris-(1-methylpyridinium-4-yl)porphyrin with calf thymus DNA

2009 ◽  
Vol 13 (08n09) ◽  
pp. 964-972 ◽  
Author(s):  
Hamid Dezhampanah ◽  
Abdol-Khalegh Bordbar ◽  
Shahram Tangestaninejad
Keyword(s):  
Fluorescence Emission ◽  
Resonance Light Scattering ◽  
Water Soluble ◽  
Calf Thymus Dna ◽  
External Mode ◽  
Calf Thymus ◽  
Enthalpy And Entropy Changes ◽  
Enthalpy And Entropy ◽  
Hoff Equation ◽  
Ct Dna

Binding properties of two water-soluble porphyrins, manganese(III) 5-(1-(4-carboxybutyl)pyridinium-4-yl) 10,15,20-tris(1-methylpyridinium-4-yl)porphyrin ( Mn(III)5-CBPyP ) and manganese(III) 5,10,15,20-tetrakis(1-methylpyridinium-4-yl)porphyrin ( Mn(III)TMPyP ), in the presence of various concentration of calf thymus DNA (ct-DNA), has been studied in 7.5 mM phosphate buffer, pH = 7.2 and at various temperatures by UV-vis absorption, resonance light scattering (RLS) and fluorescence spectroscopy and viscosity measurement. Optical absorption and RLS measurements have demonstrated three different species of both porphyrins form in DNA solution. The thermodynamic parameters were calculated by van't Hoff equation at various temperatures. The values of -4.89 kJ.mol-1 and +65.98 J.mol-1.K-1 for Mn(III)5-CBPyP and -14.92 kJ.mol-1 and +15.46 J mol-1.K-1 for TMPyP were estimated for enthalpy and entropy changes of interaction, respectively. The data indicate that the process is exothermic and enthalpy- and entropy-driven, suggesting that electrostatic forces play a considerable role in the interaction process. The binding of both porphyins to DNA quenches fluorescence emission of ethidium bromide (EB) and the quenching process obeys linear Stern-Volmer relationship, indicating the quenching of electron transfer of EB from its binding sites by these porphyrins. The results of using these techniques indicate the external mode of binding for both porphyrins and a higher binding affinity of Mn(III)5-CBPyP with respect to Mn(III)TMPyP .

Synthesis of tetra(trimethylammonio)phthalocyanato zinc tetraiodide, [ZnPc(NMe3)4]I4, and a spectrometric investigation of its interaction with calf thymus DNA

2009 ◽  
Vol 13 (12) ◽  
pp. 1255-1261 ◽  
Author(s):  
Wubiao Duan ◽  
Zhenxin Wang ◽  
Michael J. Cook
Keyword(s):  
Phosphate Buffer Solution ◽  
Binding Constants ◽  
Calf Thymus Dna ◽  
Buffer Solution ◽  
Solution Ph ◽  
Step Procedure ◽  
Calf Thymus ◽  
Electrostatic Binding ◽  
High Concentrations ◽  
Ct Dna

The phthalocyanine salt [ ZnPc(NMe3)4]I4 was synthesized from 4-nitrophthalonitrile using a three-step procedure. The interaction of [ ZnPc(NMe3)4]4+ with calf thymus DNA (CT DNA) has been investigated by UV-vis and fluorescence spectrometric methods. [ ZnPc(NMe3)4]4+ exists in a non-monomeric form, proposed to be a dimer, in phosphate buffer solution (pH 6.82). Spectral changes show that in the presence of high concentrations of CT DNA added to the solution, [ ZnPc(NMe3)4]4+ is bound in a monomeric state with evidence suggesting it is located in a DNA groove. At lower concentrations of DNA there is evidence of stacking of non-monomeric [ ZnPc(NMe3)4]4+ onto the DNA. Two intrinsic binding constants for the interaction of [ ZnPc(NMe3)4]4+ with CT DNA, 1.33 × 105 and 2.56 × 104 M-1 have been obtained. Electrostatic binding is shown to play an important role in the interaction of [ ZnPc(NMe3)4]4+ with nucleic acids.

The Use Of Molecular Docking And Spectroscopic Methods For Investigation Of The Interaction Between Regorafenib With Human Serum Albumin (HSA) And Calf Thymus DNA (Ct-DNA) In The Presence Of Different Site Markers

2020 ◽  
Vol 27 ◽  
Author(s):  
Hamid Tanzadehpanah ◽  
Hanie Mahaki ◽  
Mohammadreza Moradi ◽  
Saeid Afshar ◽  
Neda Hosseinpour Moghadam ◽  
...  
Keyword(s):  
Molecular Docking ◽  
Human Serum Albumin ◽  
Zeta Potential ◽  
Serum Albumin ◽  
Human Serum ◽  
Calf Thymus Dna ◽  
Static Mode ◽  
Calf Thymus ◽  
Site Markers ◽  
Ct Dna

Background: Interactions of drugs with DNA and proteins may modify their biological activities and conformations, which effect transport and biological metabolism of drugs. Objective: In this study the interaction of anticancer drug regorafenib (REG) with calf thymus-DNA (ct-DNA) and human serum albumin (HSA) has been investigated. Methods: Hence, for the first time, it was discovered interaction between REG with DNA and HSA using multispectroscopic, zeta potential measurements and molecular docking method. Results and Discussion: DNA displacement studies showed that REG does not have any effect on acridine orange and methylene blue bound DNA, though it was substantiated by displacement studies with Hoechst (as groove binder). Furthermore, the different concentrations of REG induce slight changes in the viscosity of ct-DNA. Zeta potential parameters indicated that hydrophobic interaction plays a major role in the DNA-REG complex. Results obtained from molecular docking demonstrate that the REG prefers to bind on the minor groove of DNAs than that of the major groove. Binding properties of HSA reveal that intrinsic fluorescence of HSA could be quenched by REG in a static mode. The competitive experiments in the presence of warfarin and ibuprofen (as site markers) suggested that the binding site of REG to HSA was most probably located in the subdomain IIA. Measurements of the zeta potential indicated that REG bound to HSA mainly by both electrostatic and hydrophobic interactions. It was found on docking procedures that REG could fit well into HSA subdomain IIA, which confirmed the experimental results. Conclusion: In conclusion, REG can be delivered by HSA in a circulatory system and affect DNA as potential target.

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