ct-DNA Binding and Antibacterial Activity of Octahedral Titanium (IV) Heteroleptic (Benzoylacetone and Hydroxamic Acids) Complexes

Five structurally related titanium (IV) heteroleptic complexes, [TiCl2(bzac)(L1–4)] and [TiCl3(bzac)(HL5)]; bzac = benzoylacetonate; L1–5 = benzohydroximate (L1), salicylhydroximate (L2), acetohydroximate (L3), hydroxyurea (L4), and N-benzoyl-N-phenyl hydroxylamine (L5), were used for the assessment of their antibacterial activities against ten pathogenic bacterial strains. The titanium (IV) complexes (1–5) demonstrated significant level of antibacterial properties as measured using agar well diffusion method. UV-Vis absorption spectroscopic technique was applied, to get a better insight into the nature of binding between titanium (IV) complexes with calf thymus DNA (ct-DNA). On the basis of the results of UV-Vis absorption spectroscopy, the interaction between ct-DNA and the titanium (IV) complexes is likely to occur through the same mode. Results indicated that titanium (IV) complex can bind to calf thymus DNA (ct-DNA) via an intercalative mode. The intrinsic binding constant (Kb) was calculated by absorption spectra by using Benesi-Hildebrand equation. Further, Gibbs free energy was also calculated for all the complexes.

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Antibacterial properties of an Indian traditional medicinal plant, Rhynchosia scarabaeoides (L.) DC. (Fabaceae)

Alternative Medicine Studies ◽

10.4081/ams.2011.e17 ◽

2011 ◽

Vol 1 (1) ◽

pp. 17 ◽

Cited By ~ 1

Author(s):

Surekha Challa ◽

Kiran K. Rajam ◽

Vishnu V. V. Satyanarayana Kasapu ◽

Suresh Kumar Tanneeru ◽

Venkata Siva Satyanarayana Kantamreddi

Keyword(s):

Ethyl Acetate ◽

Bacterial Infections ◽

Antibacterial Properties ◽

Inhibition Zone ◽

Antibacterial Activities ◽

Traditional Healers ◽

Diffusion Method ◽

Bacterial Strains ◽

Plant Parts ◽

Ethanol Extracts

Rhynchosia scarabaeoides (L.) DC plant parts are extensively used by traditional healers in India to treat a variety of bacterial diseases, such as dysentery, diarrohea and skin disorders. This article reports the antibacterial activities of n-hexane, ethyl acetate and ethanol extracts belonging to the leaf, stem and root parts of R. scarabaeoides against five bacterial strains, Bacillus subtilis, Escherichia coli, Klebseilla pneumonia, Proteus vulgaris and Staphylococcus aureus, using an agar gel diffusion method. The range of inhibition zone (IZ) was found to be 15-24 mm and the minimum inhibitory activity (MIC) was found to be 1 mL/well. The IZ was found to be higher in ethyl acetate extracts while this was moderate in ethanol extracts, and no activity was seen with n-hexane extracts or root extracts. The MIC value of leaf ethyl acetate extract was found to be 1 mg against bacterial strains P. vulgaris and S. aureus, whereas 2 mg was found against B. subtilis, K. pneumoniae and E. coli. These results support the traditional usage of R. scarabaeoides plant parts in the treatment of bacterial infections. Interestingly, this plant was screened for antibacterial activity for the first time and was found to be active. Detailed chemical investigations are, therefore, warranted.

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Synthesis, Characterization and Bioactivity of Three Carboxylic Arylhydrazone Compounds

Journal of the chemical society of pakistan ◽

10.52568/000611 ◽

2020 ◽

Vol 42 (1) ◽

pp. 149-149

Author(s):

Fengying Chen Fengying Chen ◽

Wangting Wu Wangting Wu ◽

Zhenguo Jin Zhenguo Jin ◽

Shuiyang He Shuiyang He ◽

Chengfang Qiao and Fei Yuan Chengfang Qiao and Fei Yuan

Keyword(s):

Hydrogen Bonds ◽

Propionic Acid ◽

Elemental Analysis ◽

Fluorescence Spectra ◽

Antibacterial Properties ◽

Antibacterial Activities ◽

Groove Binding ◽

Calf Thymus ◽

Ct Dna ◽

Wheat Rust

Three novel carboxylic arylhydrazone compounds named 2- oxo propionic acid terephthalal acyl dihydrazone (1), 2-ketoglutaric acid terephthalal acyl dihydrazone (2) and 2-ketoglutaric acid salicyl- hydrazone (3) were prepared and characterized by elemental analysis, IR and 1H NMR. The antibacterial activities of 1 and 2 against wheat rust and coliform were investigated. The results showed that 1 had more excellent antibacterial properties than 2 against both wheat rust and coliform. In addition, the title compounds interaction with calf-thymus DNA (CT-DNA) were measured by fluorescence spectra method which indicating that they combined with CT-DNA by groove binding through hydrogen bonds.

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Synthesis, Spectroscopic, Antibacterial, Antioxidant, DNA Binding and Cleavage, Molecular Docking Studies of Ni(II) and Cu(II) Complexes of S and N Donor Schiff Base Ligands

Research Journal of Chemistry and Environment ◽

10.25303/2511rjce143152 ◽

2021 ◽

Vol 25 (11) ◽

pp. 143-152

Author(s):

S.P. Mydhili ◽

Ramana Reddy Ch Venkata ◽

B. Sireesha ◽

S. Sreekanth

Keyword(s):

Metal Complexes ◽

Analytical Techniques ◽

Docking Studies ◽

Hydrolytic Cleavage ◽

Bacterial Strains ◽

Schiff Base Ligands ◽

Intercalative Mode ◽

Calf Thymus ◽

Ct Dna ◽

Dna Binding And Cleavage

Binary metal complexes of the ligands (L), H3FPT and H3FP4MT with Ni(II) and Cu(II) metal ions (M) were synthesized and characterized by different spectral and analytical techniques. Among these complexes, Ni(II)- H3FPT complex was found to be polymeric. The ligands and their complexes inhibited the growth of gram positive and gram negative bacterial strains to a moderate extent. The antioxidant nature of ligands and complexes was also established. Intercalative mode of binding of the complexes with calf thymus (CT) DNA was proposed from electronic absorption titrations, fluorescence quenching studies and viscosity measurements. The complexes showed hydrolytic cleavage of plasmid pBR322. Docking studies of metal complexes with DNA revealed that the complexes of H3FP4MT are more active than H3FPT.

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Computational Studies of 4-Formylpyridinethiosemicarbazone and Structural and Biological Studies of Its Ni(II) and Cu(II) Complexes

Heteroatom Chemistry ◽

10.1155/2019/3507837 ◽

2019 ◽

Vol 2019 ◽

pp. 1-10

Author(s):

Mydhili P. Sripathi ◽

Sireesha Berely ◽

Chittireddy Venkata Ramana Reddy

Keyword(s):

Elemental Analysis ◽

Radical Scavenging ◽

Molar Conductance ◽

Computational Studies ◽

Hydrolytic Cleavage ◽

Bacterial Strains ◽

Intercalative Mode ◽

Biological Studies ◽

Calf Thymus ◽

The Stability

To understand the stability, chelation behaviour, and biological activity of 4-Formylpyridinethiosemicarbazone (H4FPT), it is important to recognize its interactive geometry. Hence, computational studies on geometrically optimized structures of thione and thiol forms of H4FPT were performed. Binary metal complexes of the ligand, H4FPT (L) with the Ni(II) and Cu(II) metal ions (M), were synthesized and characterized by various spectroanalytical techniques as elemental analysis, molar conductance, magnetic susceptibility measurements, LC-MS, TGA, IR, UV-Visible, ESR, and powder XRD. Elemental analysis, LC-MS, and TGA studies indicate 1:2 (ML2) composition for mononuclear Ni(II) complex and 1:1 (ML) composition for dinuclear Cu(II) complex. Electronic absorption titrations, fluorescence quenching studies, and viscosity measurements suggest intercalative mode of binding of the complexes with calf thymus DNA (CT-DNA). These complexes also promote hydrolytic cleavage of plasmid pBR322. The ligand (H4FPT) and its complexes showed moderate-to-good activity against Gram-positive and Gram-negative bacterial strains. The DPPH radical scavenging studies showed antioxidant nature of both complexes.

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Antibacterial Activity of Leukocyte- and Platelet-Rich Plasma: AnIn VitroStudy

BioMed Research International ◽

10.1155/2018/9471723 ◽

2018 ◽

Vol 2018 ◽

pp. 1-8 ◽

Cited By ~ 7

Author(s):

Agata Cieślik-Bielecka ◽

Tadeusz Bold ◽

Grzegorz Ziółkowski ◽

Marcin Pierchała ◽

Aleksandra Królikowska ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Enterococcus Faecalis ◽

Platelet Rich Plasma ◽

Antibacterial Properties ◽

Antimicrobial Effect ◽

Diffusion Method ◽

Bacterial Strains ◽

Bovine Thrombin ◽

Zones Of Inhibition

The aim of the study was to investigate the leukocyte- and platelet-rich plasma (L-PRP) antimicrobial activity. The studied sample comprised 20 healthy males. The L-PRP gel, liquid L-PRP, and thrombin samples were testedin vitrofor their antibacterial properties against selected bacterial strains using the Kirby-Bauer disc diffusion method. Two types of thrombin were used (autologous and bovine). Zones of inhibition produced by L-PRP ranged between 6 and 18 mm in diameter. L-PRP inhibited the growth ofStaphylococcus aureus(MRSA and MSSA strains) and was also active againstEnterococcus faecalisandPseudomonas aeruginosa. There was no activity againstEscherichia coliandKlebsiella pneumoniae. The statistically significant increase of L-PRP antimicrobial effect was noted with the use of major volume of thrombin as an activator. Additionally, in groups where a bovine thrombin mixture was added to L-PRP the zones of inhibition concerning MRSA,Enterococcus faecalis, andPseudomonas aeruginosawere larger than in the groups with autologous thrombin. Based on the conducted studies, it can be determined that L-PRP can evokein vitroantimicrobial effects and might be used to treat selected infections in the clinical field. The major volume of thrombin as an activator increases the strength of the L-PRP antimicrobial effect.

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Chemical composition and antibacterial activities of essential oils from fruits of Melicope pteleifolia (Champ. Ex Benth.) T.G. Hartley grown in Lam Dong Province, Vietnam

ACADEMIA JOURNAL OF BIOLOGY ◽

10.15625/2615-9023/v42n3.13916 ◽

2020 ◽

Vol 42 (3) ◽

Author(s):

Hoang Thi Binh ◽

Tran Thi Bao Tram ◽

Do Ngoc Dai ◽

Vuong Thuy Tien ◽

Le Minh Tam ◽

...

Keyword(s):

Mass Spectrometry ◽

Chemical Composition ◽

Essential Oil ◽

Streptococcus Pyogenes ◽

Antibacterial Properties ◽

Antibacterial Activities ◽

Gas Chromatography Mass Spectrometry ◽

Diffusion Method ◽

Aerial Parts ◽

Agar Well Diffusion Method

In the present study, chemical composition and antibacterial properties of essential oil obtained from the aerial parts of the Melicope pteleifolia (Champ. ex Benth.) T.G Hartley in Dalat were evaluated. Essential oil was isolated through hydro-distillation. Twenty-nine constituents comprising 100% of the essential oil were characterized by gas chromatography/mass spectrometry (GC-MS) techniques. The major compounds in the essential oil were (+)-Sabinene (34.73%), Cis-α-bergamotene (13.15%), Z-α-trans-bergamotol (5.28%), β-mycrene (4.98%), and 1,3,6-octatriene, 3,7-dimethyl-(4.71%). Antibacterial activities of Melicope pteleifolia essential oil were investigated against Gram-positive and-negative bacteria. Results showed significant activities against Streptococcus pyogenes and Escherichia coli using an agar well diffusion method. The application of this essential oil in preventing and eliminating bacteria could be useful in fields as medicine and cosmetics.

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Mechanical and antibacterial properties of ZnO/chitosan bio-composite films

Journal of Polymer Engineering ◽

10.1515/polyeng-2021-0143 ◽

2021 ◽

Vol 0 (0) ◽

Author(s):

Ayse Kalemtas ◽

Hasan B. Kocer ◽

Ahmet Aydin ◽

Pinar Terzioglu ◽

Gulsum Aydin

Keyword(s):

Particle Size ◽

Food Packaging ◽

Mechanical Test ◽

Composite Films ◽

Antibacterial Properties ◽

Chitosan Film ◽

Antibacterial Activities ◽

Diffusion Method ◽

Zno Powders ◽

Solution Casting Method

Abstract In the current study, ZnO/chitosan bio-composite films were produced via solution-casting method. Two different ZnO powders, micrometer (d50 ≅ 1.5 μm) and nanometer sized (d50 ≅ 100 nm), were used to investigate the effect of ZnO particle size and concentration (0, 2, and 8% w/w of chitosan) on the mechanical and antibacterial properties of the ZnO/chitosan bio-composite films. The incorporation of the ZnO powders into the chitosan film resulted in an increase in the tensile strength (TS) and a decrease in the elongation at break (EB) values. Mechanical test results revealed that TS and EB properties were considerably affected (p < 0.05) by the concentration and particle size of the ZnO reinforcement. Disc diffusion method demonstrated good antibacterial activities of bio-composite films containing high amount of ZnO (8% w/w of chitosan) against Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, and Bacillus subtilis. The growth-limiting effect of the films was more pronounced for S. aureus and K. pneumoniae. Due to enhanced TS and imparted antibacterial activity of the produced ZnO/chitosan bio-composite films, these materials are promising candidates for applications such as food packaging, wound dressing, and antibacterial coatings for various surfaces.

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Cu(ii)-induced twisting of the biphenyl core: exploring the effect of structure and coordination environment of biphenyl-based chiral copper(ii) complexes on interaction with calf-thymus DNA

New Journal of Chemistry ◽

10.1039/c9nj06184c ◽

2020 ◽

Vol 44 (46) ◽

pp. 20275-20284

Author(s):

Soumen Ghosh ◽

Mehebub Ali Khan ◽

Arghyadeep Bhattacharyya ◽

Md. Akhtarul Alam ◽

Ennio Zangrando ◽

...

Keyword(s):

Optically Active ◽

Calf Thymus Dna ◽

Coordination Environment ◽

Calf Thymus ◽

Ct Dna ◽

Effect Of Structure

Biphenyl core-based clip-like receptors get twisted after complexation with Cu2+. The extent of interaction of the optically active complexes with ct-DNA varies depending on the structure and coordination environment.

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Phytochemical Study and Antibacterial and Antibiotic Modulation Activity of Punica granatum (Pomegranate) Leaves

Scientifica ◽

10.1155/2020/8271203 ◽

2020 ◽

Vol 2020 ◽

pp. 1-7

Author(s):

Amine Trabelsi ◽

Mohamed Amine El Kaibi ◽

Aïmen Abbassi ◽

Amira Horchani ◽

Leila Chekir-Ghedira ◽

...

Keyword(s):

Antibacterial Properties ◽

Punica Granatum ◽

Diffusion Method ◽

Bacterial Strains ◽

Leaf Extracts ◽

Phytochemical Study ◽

E Coli ◽

Microdilution Method ◽

Resistant Strains

This study aimed to determine phytochemical contents, antibacterial properties, and antibiotic modulating potential of Punica granatum leaf extracts: hexane, chloroform, ethyl acetate, ethanol, and aqueous extracts as well as an extract enriched with total oligomer flavonoids (TOFs). The TOF extract contained the highest value of phenols and flavonoids. Rutin, luteolin, gallic acid, and ellagic acid were determined by HPLC analysis of this extract. The antibacterial activity was assayed by the disc diffusion method and microdilution method against Staphylococcus aureus and Escherichia coli standard ATCC strains and clinical isolates resistant strains. The TOF extract was the most active against all tested strains. The checkerboard method was used for the determination of synergy between two antibiotics (amoxicillin and cefotaxime) and P. granatum leaf extracts. The best synergistic interaction was found with TOF extract combined with amoxicillin for penicillin-resistant E. coli and penicillin-resistant S. aureus. These results can be assigned to tannins, flavonoids, and phenolic acids found in P. granatum leaf extracts. Pomegranate leaf extracts or active compounds isolated from these extracts could be used to fight the emergence and spread of resistant bacterial strains.

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Antibacterial Potential of Aloe weloensis (Aloeacea) Leaf Latex against Gram-Positive and Gram-Negative Bacteria Strains

International Journal of Microbiology ◽

10.1155/2019/5328238 ◽

2019 ◽

Vol 2019 ◽

pp. 1-4

Author(s):

Yohannes Kelifa Emiru ◽

Ebrahim Abdela Siraj ◽

Tekleab Teka Teklehaimanot ◽

Gedefaw Getnet Amare

Keyword(s):

Antibacterial Activity ◽

Bacterial Infections ◽

Inhibition Zone ◽

Antibacterial Activities ◽

Maximum Activity ◽

New Drugs ◽

Diffusion Method ◽

Bacterial Strains ◽

Gram Positive ◽

Gram Negative

Objective. To evaluate the antibacterial effects of the leaf latex of Aloe weloensis against infectious bacterial strains. Methods. The leaf latex of A. weloensis at different concentrations (400, 500, and 600 mg/ml) was evaluated for antibacterial activities using the disc diffusion method against some Gram-negative species such as Escherichia coli (ATCC 14700) and Pseudomonas aeruginosa (ATCC 35619) and Gram-positive such as Staphylococcus aureus (ATCC 50080) and Enterococcus fecalis (ATCC 4623). Results. The tested concentrations of the latex ranging between 400 and 600 mg·mL−1 showed significant antibacterial activity against bacterial strain. The highest dose (600 mg/ml) of A. weloensis leaf latex revealed the maximum activity (25.93 ± 0.066 inhibition zone) followed by the dose 500 mg/ml against S. aureus. The lowest antibacterial activity was observed by the concentration 400 mg/ml (5.03 ± 0.03) against E. coli. Conclusion. The results of the present investigation suggest that the leaf latex of A. weloensis can be used as potential leads to discover new drugs to control some bacterial infections.

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