Species-Specificity of an Elicitor-Active Oligosaccharide, LN-3, to Leguminous Plants

1996 ◽  
Vol 51 (5-6) ◽  
pp. 371-378 ◽  
Author(s):  
Akihiro Tai ◽  
Kazuyoshi Kawazu ◽  
Akio Kobayashi

Abstract LN-3, a linear pyridylaminated hepta-β-glucoside previously found to show elicitor activity in alfalfa cotyledons, was examined for phytoalexin-inducing activity in pea epicotyl and bean cotyledon assays. LN-3 did not show (+)-pisatin-inducing activity in pea epicotyls. In the bean cotyledon assay, however, the (± )-kievitone content gradually increased with increasing LN- 3 concentration, and reached a maxim um (ca. 17 |ig/g fresh wt) at 100 μ/ml. Half-maximal elicitor activity was seen at ca. 16 μm . After three legumes, alfalfa, pea and bean, were treated with LN-3, the recovery of the rem aining LN-3 or its fragments was examined. Almost 100% of LN-3 or its fragments was recovered from the pea test solution; in contrast, recoveries from alfalfa and bean were only 63.8 and 38.1% , respectively. HPLC and LC-MS analyses of the recovered samples indicated that LN-3 was hydrolyzed to give mono- and/or diglucoside( s) in the alfalfa and the bean solutions, while in the pea experiment a small portion of LN-3 was hydrolyzed to give sugar fragments with different degrees of polymerization < 7.

1968 ◽  
Vol 20 (03/04) ◽  
pp. 457-464 ◽  
Author(s):  
L Gonyea ◽  
R Herdman ◽  
R. A Bridges

SummaryAn anticoagulant occurring in 4 of 6 patients with SLE has been demonstrated by a sensitive assay utilizing an ammonium sulfate fraction of serum. The anticoagulant functions as an inhibitor of the activation of prothrombin. No species specificity was demonstrable. The inhibitor behaves clinically and chromatographically as an immunoglobulin, although an attempt to demonstrate directly the antibody nature of the inhibitor was not successful.A severe, apparently independent, decrease in the level of prothrombin was observed in the patient with hemorrhagic symptoms. In contrast to the anticoagulant activity, the low prothrombin has persisted during treatment.


2019 ◽  
Author(s):  
Khurshida Salavatova

The article covers agrotechnology of cultivation of herbicides in theKhorezm region and raising the fertility of the leguminous plant,which is a leguminous plant and the solution of the problem ofprotein deficiency consumed by the population.


2020 ◽  
pp. 13-23
Author(s):  
Natalya Kornova ◽  
A. Krylov

The article presents results of a study on the effectiveness of using lowfrequency ultrasound and photochromotherapy in the complex treatment of patients with chronic bacterial and viral rhinosinusitis. The study involved 69 people aged 18 to 55 years: 39 (56.83 %) women and 30 (45.11 %) men with chronic rhinosinusitis during the period of clinical exacerbation and concomitant chronic tonsillitis without exacerbation. All patients included in the study underwent X-ray examination or computed tomography and magnetic resonance imaging of the paranasal sinuses. 100 % of the patients were examined for the presence of antibodies to Human gammaherpesvirus 4 (ELISA), had study of blood serum for IgM and IgG and test to determine the avidity of Ig G antibodies to γ-herpesviruses. The obtained data indicate the effectiveness of including low-frequency ultrasound and photochromotherapy in the complex treatment of patients with chronic rhinosinusitis and concomitant chronic tonsillitis of bacterial and viral nature without exacerbation.


2010 ◽  
Vol 18 (1) ◽  
pp. 1 ◽  
Author(s):  
Chen Yan ◽  
Li Hongqing ◽  
Liu Min ◽  
Chen Xiaoyong
Keyword(s):  

Pathogens ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 465
Author(s):  
Gregorio P. Milani ◽  
Mario G. Bianchetti ◽  
Giuseppe Togni ◽  
Andreas W. Schoenenberger ◽  
Franco Muggli

It is assumed that healthcare workers are at the highest risk to be infected by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). However, few data from healthcare workers who do not primarily take care of patients with SARS-CoV-2 infection support this assumption. We investigated the prevalence of immunoglobulin G (Ig G) against SARS-CoV-2 among healthcare workers who do not primarily take care of patients with SARS-CoV-2 infection and the general population in a well-defined geographical area. The first part of the study was conducted in May 2020 in Val Mesolcina (Southern Switzerland), a valley with ~8000 inhabitants. All healthcare workers were invited. All participants (n = 488) of the Swiss Longitudinal Cohort Study (SWICOS), a cohort representative of the general population, were also invited. Circulating Ig G against spike protein subunit 1 of SARS-CoV-2 were tested in each subject. Subjects with positive Ig G were tested again after 6 months. The condition of being a healthcare worker, rather than a part of the general population, was tested as a predictor of seroprevalence positivity by both simple and multiple (adjusted for age and sex) logistic regression. Eleven (2.6%) of the 423 SWICOS participants and 46 (16%) out of 289 healthcare workers were positive for antibodies against SARS-CoV-2. The seroprevalence OR was 7.01 (95% CI: 3.53–15.47) for healthcare workers as compared to SWICOS participants. After adjusting for age and gender, the seroprevalence OR was 5.13 (95% CI: 2.54–10.40). About three quarters of the subjects in the SWICOS (73%) and in healthcare (79%) group with a previous positive serology still presented positive Ig G against the SARS-CoV-2 after 6 months. The present seroprevalence data point out that the SARS-CoV-2 infection is seven times higher among healthcare workers than in the general population of Val Mesolcina. Efforts to effectively protect all the healthcare personnel are needed.


Antibiotics ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 930
Author(s):  
Alberto Corona ◽  
Giuseppe Richini ◽  
Sara Simoncini ◽  
Marta Zangrandi ◽  
Monica Biasini ◽  
...  

SARS-CoV-2 in patients who need intensive care unit (ICU) is associated with a mortality rate ranging from 10 to 40–45%, with an increase in morbidity and mortality in presence of sepsis. We hypothesized that IgM and IgA enriched immunoglobulin G may support the sepsis-related phase improving patient outcome. We conducted a retrospective case–control study on 47 consecutive patients admitted to our ICU. At the time of admission, patients received anticoagulants (heparin sodium) together with the standard supportive treatment. We decided to add IgM and IgA enriched immunoglobulin G to the standard therapy. Patients receiving IgM and IgA enriched immunoglobulin G were compared with patients with similar baseline characteristics and treatment, receiving only standard therapy. The mortality resulted significantly higher in patients treated with standard therapy only (56.5 vs. 37.5%, p < 0.01) and, at day 7, the probability of dying was 3 times higher in this group. Variable life adjustment display (VLAD) was 2.4 and -2.2 (in terms of lives saved in relation with those expected and derived from Simplified Acute Physiology Score II) in the treated and not treated group, respectively. The treatment based on IgM and IgA enriched immunoglobulin G infusion seems to give an advantage on survival in SARS-CoV-2 severe infection.


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