Transgenic Potato Plants Expressing Soybean ß-1,3-Endoglucanase Gene Exhibit an Increased Resistance to Phytophthora infestans

1998 ◽  
Vol 53 (11-12) ◽  
pp. 1012-1016 ◽  
Author(s):  
Maria Borkowska ◽  
Magdalena Krzymowska ◽  
Andrzej Talarczyk ◽  
Malik F. M. Awan ◽  
Ludmila Yakovleva ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Phytophthora Infestans ◽  
Cell Walls ◽  
Fungal Pathogens ◽  
Pcr Amplification ◽  
Transgenic Potato ◽  
Fungal Cell ◽  
Potato Genome ◽  
Potato Plants ◽  
Transgenic Potato Plants

Abstract Soybean β-1,3-endoglucanase represents a model system for studies on early plant re­sponses to infection by fungal pathogens, and it has been implicated in the release of elicitors from fungal cell walls. In the present study, potato plants were transformed with the soybean β-1,3-endoglucanase cDNA via Agrobacterium delivery system. The transfer of the gene into potato genome was confirmed by (i) PCR amplification, (ii) Northern blot analyses, and (Hi) an increase in the activity of β-1,3-endoglucanase in transgenic plants. The transformation resulted in an increased resistance of selected transgenic plants to infection by Phytophthora infestans, an important pathogen.

scholarly journals Copper and nickel resistance at ipt-transgenic potato plants

2004 ◽  
Vol 2 (3) ◽  
pp. 25-31
Author(s):  
Elena A Andreeva ◽  
Ludmila A Lutova
Keyword(s):  
Transgenic Plants ◽  
Copper Sulfate ◽  
Transgenic Potato ◽  
Nickel Chloride ◽  
Nickel Resistance ◽  
Potato Plants ◽  
Agrobacterial Transformation ◽  
Enhanced Resistance ◽  
Transgenic Potato Plants

Using agrobacterial transformation collection of /pr-transgenic plants based on cv. Adretta was obtained. Analysis of transgenic plants susceptibility to the exogenous phytohormones auxin (IAA) and cytokinin (kinetin) for 18 from 22 analyzed forms reveals altered reaction to hormones. Among 18 plants with altered reaction to phytohormones for 16 forms enhanced resistance to copper sulfate and/or nickel chloride was observed

RESISTANCE OF TRANSGENIC POTATOES TO ATTACK BY EPITRIX CUCUMERIS (COLEOPTERA: CHRYSOMELIDAE)

1999 ◽  
Vol 131 (4) ◽  
pp. 423-431 ◽  
Author(s):  
Jeff G. Stewart ◽  
Jennifer Feldman ◽  
Debby A. LeBlanc
Keyword(s):  
Transgenic Plants ◽  
Prince Edward Island ◽  
Flea Beetle ◽  
Transgenic Potato ◽  
Growth Stages ◽  
Potato Plants ◽  
Transgenic Potato Plants ◽  
Transgenic Potatoes ◽  
Choice Feeding ◽  
Potato Flea Beetle

AbstractPotato plants (Solanum tuberosum, cv. Russet Burbank) genetically altered to produce the CryIIIA protein from Bacillus thuringiensis var tenebrionis were tested for susceptibility to attack by the potato flea beetle, Epitrix cucumeris (Harris), on Prince Edward Island, Canada, from 1993 to 1996. Average weekly damage to the fourth terminal leaf of transgenic potato plants was 31% lower in 1993 and 20% lower in 1994 compared with nontransgenic plants. Transgenic plants had 29% fewer potato flea beetle adults when compared with nontransgenic plants in 1994, although no difference was detected between the two plant types in 1993. Recovery of potato flea beetle pupae in the soil was noticeably lower from transgenic plants expressing the CryIIIA protein in all parts of the plant (Construct No. RBBT02-10Y1) than from either the transgenic plants that expressed the CryIIIA protein primarily in the green foliage (Construct No. RBBT04-01) or the nontransgenic plants. Adult potato flea beetles, when given a choice between transgenic and nontransgenic leaf material, fed preferentially on nontransgenic material. In contrast, when adults were not given a choice, feeding damage was similar between treatments. These results suggest that the reason for reduced feeding on transgenic potato plants by the potato flea beetle is, at least in part, due to some toxicity of the plants to immature growth stages and a preference for adults to feed on nontransgenic material when it is available.

Transgenic 14-3-3 isoforms in plants: the metabolite profiling of repressed 14-3-3 protein synthesis in transgenic potato plants

2002 ◽  
Vol 30 (4) ◽  
pp. 405-410 ◽  
Author(s):  
J. Szopa
Keyword(s):  
Transgenic Plants ◽  
Soluble Sugars ◽  
Transgenic Potato ◽  
Tuber Size ◽  
Tuber Number ◽  
Catecholamine Level ◽  
Potato Plants ◽  
Transgenic Potato Plants ◽  
Nr Activity

14-3-3 proteins are abundant eukaryotic proteins that interact with many other proteins, thereby modulating their function and thus cell metabolism. The data from mRNA analysis confirm the developmental regulation of 14-3-3 isoform expression in potato plants. In order to test whether or not 14-3-3 protein expression affects plant phenotype and metabolism, transgenic potato plants either overexpressing Cucurbita pepo 14-3-3 or underexpressing endogenous 14-3-3 isoforms were analysed. An increase in tuber number and a decrease in tuber size in the over-expressed transformant was observed; the transgenic plants contain more chlorophyll than the control and they lose it more slowly than the control when transferred to the dark. The 14-3-3-repressed transgenic plants showed a decrease in tuber number and an increase in tuber size; an increase in the fresh weight of the transgenic tubers was also detected. The increased catecholamine level was accompanied by an increased ratio of soluble sugars to starch in overexpressed transformant. The opposite effect was detected in 14-3-3-repressed transgenic plants. All the repressed plants showed significant increases in nitrate reductase (NR) activity, suggesting that the regulation of NR occurs in vivo, and is not isoform-dependent. The increase in NR activity resulted in a significant decrease in nitrate level. The level of sucrose phosphate synthase activity was also significantly increased in all 14-3-3-underexpressed transgenes, and remarkably the increase in enzyme activity was accompanied by respective changes in sucrose levels in the tubers. The most intriguing finding was the significant (2–3-fold) increase in ethylene content in all the 14-3-3-repressed transgenic lines, which probably resulted from a methionine level increase. The substantial increase of ethylene level in the repressed forms might explain the significant shortening of the vegetation period of the analysed transgenic plants.

scholarly journals Obtaining of transgenic potato plants (Solanum tuberosum L.) that contain antisense sequence of prolindehydrogenase gene

2018 ◽  
Vol 22 ◽  
pp. 299-304
Author(s):  
O. O. Ovcharenko ◽  
V. A. Rudas ◽  
N. L. Shcherbak ◽  
M. V. Kuchuk
Keyword(s):  
Solanum Tuberosum ◽  
Transgenic Plants ◽  
Genetic Transformation ◽  
Solanum Tuberosum L ◽  
Practical Interest ◽  
Transgenic Potato ◽  
Proline Dehydrogenase ◽  
Potato Plants ◽  
Antisense Sequence ◽  
Transgenic Potato Plants

Aim. The aim of our work was to obtain transgenic potato plants of Ukrainian varieties with the expression of a double-stranded RNA suppressor of proline dehydrogenase gene. We propose the decrease of proline degradation level and increase of overall proline concentration in obtained transgenic plants. Methods. The Agrobasterium tumefaciens-mediated method of genetic transformation to obtain transgenic plants of potato was used. Internodes of aseptic potato plants were transformed with a binary vector pBi2E containing an inverted repeats of two copies of proline dehydrogenase gene’s first exon and the gene of neomycin phosphotransferase II (nptII). Results. As a result of experiments kanamycin resistant transgenic potato lines of Deseiree, Belarusian 12 and Slavianka varieties were obtained. The transgenic nature of the obtained plants was confirmed by PCR with primers specific to the first exon of proline dehydrogenase and to nptII genes. Conclusions. The optimized conditions of genetic transformation and used agrobacterial strain allow to obtain the transgenic plants of a model potato variety Désirée, as well as varieties Belorussian 12 and Slovyanka which are of practical interest for cultivation in Ukraine. Keywords: transgenic plants, potatoes (Solanum tuberosum L.), stress resistance, proline.

scholarly journals POTENTIAL OF PROTEINASE INHIBITORS FOR THE PRODUCTION OF TRANSGENIC PLANTS TOLERANT TO INSECT PESTS. I. FUNCTIONAL ASPECTS

HortScience ◽  
1994 ◽  
Vol 29 (4) ◽  
pp. 249e-249
Author(s):  
Dominique Michaud ◽  
Serge Overney ◽  
Binh Nguyen-Quoc ◽  
Serge Yelle
Keyword(s):  
Transgenic Plants ◽  
Insect Pests ◽  
Proteinase Inhibitors ◽  
Transgenic Potato ◽  
Control Approach ◽  
Potato Plants ◽  
Potato Beetle ◽  
Functional Aspects ◽  
Digestive Proteinases ◽  
Transgenic Potato Plants

In the past few years, transformation of plant genomes with proteinase inhibitor (PI) genes has been proposed as an effective way to produce insect-tolerant plants. For such a control approach, however, biochemical studies are necessary to assess the effect of PIs on not only insect digestive proteinases (target enzymes) but also plant endogenous proteinases (nontarget enzymes). As an example, transformation of potato (Solanum tuberosum L.) with oryzacystatin (OC) genes, two cysteine PIs, was considered for controlling Colorado potato beetle (CPB; Leptinotarsa decemlineata Say). The use of electrophoretic approaches and standard assays showed that CPB uses at least 14 cysteine proteinases for protein digestion throughout its development. Proteinases of the same class were also detected in sprouting potato tuber extracts, suggesting a potential interference of cPIs in transgenic plants. While OCs inhibit a significant fraction of CPB digestive proteinases, no inactivation of potato proteinases was detected. This apparent absence of direct interference suggests the real potential of OCs for producing CPB-tolerant transgenic potato plants.

scholarly journals Activation of the peroxidase gene in potato plants expressing the gene of antimicrobial peptide MsrA1

2020 ◽  
Vol 64 (3) ◽  
pp. 325-331
Author(s):  
A. Yu. Misyukevich ◽  
T. A. Gapeeva ◽  
T. G. Tretyakova ◽  
T. V. Semanyuk ◽  
I. D. Volotovski
Keyword(s):  
Antimicrobial Peptide ◽  
Fungal Pathogens ◽  
Indirect Evidence ◽  
Alternaria Solani ◽  
Transgenic Potato ◽  
Peroxidase Gene ◽  
Potato Plants ◽  
Silk Moth ◽  
Expression Studies ◽  
Transgenic Potato Plants

Antimicrobial peptide MsrA1 is a synthetic hybrid molecule based on cecropin A from giant silk moth larvae and on melittin from melliferous bee venom. Transgenic potato plants of the Belarusian variety Odyssey with the constitutive expressiоn of msrA1 gene are shown to exhibit increased resistance to fungal pathogens Phytophthora infestans and Alternaria solani. Peroxidase genes expression studies using cDNA-PCR and DNA sequencing revealed the activation of the POX peroxidase gene in transgenic plants in the absence of pathogens. This may be indirect evidence of the increased formation of reactive oxygen species, which may explain special resistance to fungal pathogens. The data obtained also confirm a possible role of intracellular antimicrobial peptide in making the plants more resistant to oxidative stress by the way of activation of the host plant defense system.

Overexpression of Solanum tuberosum respiratory burst oxidase homolog A (StRbohA) promotes potato tolerance to Phytophthora infestans.

2021 ◽  
Author(s):  
Atta Soliman ◽  
Lorne R. Adam ◽  
Pawanpuneet K. Rehal ◽  
Fouad Daayf
Keyword(s):  
Solanum Tuberosum ◽  
Transgenic Plants ◽  
Phytophthora Infestans ◽  
Respiratory Burst ◽  
Defense Mechanisms ◽  
Transgenic Potato ◽  
Plant Tolerance ◽  
Potato Plants ◽  
Pathogenesis Related ◽  
Respiratory Burst Oxidase

Reactive oxygen species (ROS) represent one of the first lines of plants’ biochemical defense against pathogens. Plants’ respiratory burst oxidase homologs (RBOHs) produce ROS as by-products in several cellular compartments. In potato tubers, Solanum tuberosum respiratory burst oxidase homolog (StRBOHs) are involved in suberization and healing of wounded tissues. StRbohA has been tested in the model plant Arabidopsis thaliana, which led to enhanced plant defense against the soil-borne pathogen Verticillium dahliae. Here, we showed that overexpressing StRbohA in potato plants enhancesd plant tolerance to the oomycete Phytophthora infestans, the causal agent of late blight disease. Transgenic potato plants expressing StRbohA showed reduced disease symptoms (necrosis) compared to the wild type check. The In parallel, the expression of pathogenesis-related genes (PRs), RBOHs, antioxidation-related genes CPRX1, PRX2, APRX1, CAT1, and CAT2, and genes involved in the biosynthesis pathways of jasmonic and salicylic acids (ICS, PAL1, PAL2, LOX1, LOX2, and LOX3) exhibited significant increases in the transgenic plants in response to infection. Following higher expression of RBOHs, ROS accumulated more in inoculation sites of the transgenic plants. ROS act as signals that activate gene expression in the SA biosynthesis pathway, leading to the accumulation of SA and triggering SA-based defense mechanisms. SA-responsive pathogenesis-related genes (PRs) showed higher expression in the transgenic plants, which resulted in the restriction of pathogen growth in plant tissues. These results represent a demonstration of the effective role of StRbohA in enhancing potato defense against P. infestans.

Expression of the MSI-99m Gene in Transgenic Potato Plants Confers Resistance to Phytophthora infestans and Ralstonia solanacearum

2012 ◽  
Vol 31 (2) ◽  
pp. 418-424 ◽  
Author(s):  
Yong-bo Hong ◽  
Shui-ping Liu ◽  
Yan-ping Zhu ◽  
Chao Xie ◽  
Deng-wei Jue ◽  
...  
Keyword(s):  
Phytophthora Infestans ◽  
Ralstonia Solanacearum ◽  
Transgenic Potato ◽  
Potato Plants ◽  
Transgenic Potato Plants

Germin-like protein 2 gene promoter from rice is responsive to fungal pathogens in transgenic potato plants

2015 ◽  
Vol 16 (1) ◽  
pp. 19-27 ◽  
Author(s):  
Faiza Munir ◽  
Satomi Hayashi ◽  
Jacqueline Batley ◽  
Syed Muhammad Saqlan Naqvi ◽  
Tariq Mahmood
Keyword(s):  
Fungal Pathogens ◽  
Gene Promoter ◽  
Transgenic Potato ◽  
Potato Plants ◽  
Transgenic Potato Plants
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