Transgenic 14-3-3 isoforms in plants: the metabolite profiling of repressed 14-3-3 protein synthesis in transgenic potato plants

14-3-3 proteins are abundant eukaryotic proteins that interact with many other proteins, thereby modulating their function and thus cell metabolism. The data from mRNA analysis confirm the developmental regulation of 14-3-3 isoform expression in potato plants. In order to test whether or not 14-3-3 protein expression affects plant phenotype and metabolism, transgenic potato plants either overexpressing Cucurbita pepo 14-3-3 or underexpressing endogenous 14-3-3 isoforms were analysed. An increase in tuber number and a decrease in tuber size in the over-expressed transformant was observed; the transgenic plants contain more chlorophyll than the control and they lose it more slowly than the control when transferred to the dark. The 14-3-3-repressed transgenic plants showed a decrease in tuber number and an increase in tuber size; an increase in the fresh weight of the transgenic tubers was also detected. The increased catecholamine level was accompanied by an increased ratio of soluble sugars to starch in overexpressed transformant. The opposite effect was detected in 14-3-3-repressed transgenic plants. All the repressed plants showed significant increases in nitrate reductase (NR) activity, suggesting that the regulation of NR occurs in vivo, and is not isoform-dependent. The increase in NR activity resulted in a significant decrease in nitrate level. The level of sucrose phosphate synthase activity was also significantly increased in all 14-3-3-underexpressed transgenes, and remarkably the increase in enzyme activity was accompanied by respective changes in sucrose levels in the tubers. The most intriguing finding was the significant (2–3-fold) increase in ethylene content in all the 14-3-3-repressed transgenic lines, which probably resulted from a methionine level increase. The substantial increase of ethylene level in the repressed forms might explain the significant shortening of the vegetation period of the analysed transgenic plants.

Download Full-text

Thermodynamic and structural properties of tuber starches from transgenic potato plants grown in vitro and in vivo

Carbohydrate Polymers ◽

10.1016/j.carbpol.2015.01.084 ◽

2015 ◽

Vol 125 ◽

pp. 214-223 ◽

Cited By ~ 6

Author(s):

Luybov A. Wasserman ◽

Andrey I. Sergeev ◽

Viktor G. Vasil’ev ◽

Irina G. Plashchina ◽

Nina P. Aksenova ◽

...

Keyword(s):

Structural Properties ◽

Transgenic Potato ◽

Potato Plants ◽

Transgenic Potato Plants

Download Full-text

Copper and nickel resistance at ipt-transgenic potato plants

Ecological genetics ◽

10.17816/ecogen2325-31 ◽

2004 ◽

Vol 2 (3) ◽

pp. 25-31

Author(s):

Elena A Andreeva ◽

Ludmila A Lutova

Keyword(s):

Transgenic Plants ◽

Copper Sulfate ◽

Transgenic Potato ◽

Nickel Chloride ◽

Nickel Resistance ◽

Potato Plants ◽

Agrobacterial Transformation ◽

Enhanced Resistance ◽

Transgenic Potato Plants

Using agrobacterial transformation collection of /pr-transgenic plants based on cv. Adretta was obtained. Analysis of transgenic plants susceptibility to the exogenous phytohormones auxin (IAA) and cytokinin (kinetin) for 18 from 22 analyzed forms reveals altered reaction to hormones. Among 18 plants with altered reaction to phytohormones for 16 forms enhanced resistance to copper sulfate and/or nickel chloride was observed

Download Full-text

Transgenic Potato Plants Expressing Soybean ß-1,3-Endoglucanase Gene Exhibit an Increased Resistance to Phytophthora infestans

Zeitschrift für Naturforschung C ◽

10.1515/znc-1998-11-1212 ◽

1998 ◽

Vol 53 (11-12) ◽

pp. 1012-1016 ◽

Cited By ~ 21

Author(s):

Maria Borkowska ◽

Magdalena Krzymowska ◽

Andrzej Talarczyk ◽

Malik F. M. Awan ◽

Ludmila Yakovleva ◽

...

Keyword(s):

Transgenic Plants ◽

Phytophthora Infestans ◽

Cell Walls ◽

Fungal Pathogens ◽

Pcr Amplification ◽

Transgenic Potato ◽

Fungal Cell ◽

Potato Genome ◽

Potato Plants ◽

Transgenic Potato Plants

Abstract Soybean β-1,3-endoglucanase represents a model system for studies on early plant responses to infection by fungal pathogens, and it has been implicated in the release of elicitors from fungal cell walls. In the present study, potato plants were transformed with the soybean β-1,3-endoglucanase cDNA via Agrobacterium delivery system. The transfer of the gene into potato genome was confirmed by (i) PCR amplification, (ii) Northern blot analyses, and (Hi) an increase in the activity of β-1,3-endoglucanase in transgenic plants. The transformation resulted in an increased resistance of selected transgenic plants to infection by Phytophthora infestans, an important pathogen.

Download Full-text

The Overexpression of The VvWRKY2 Transcription Factor in Potato Improved the Agricultural Performance and Tubers’ Physio-Chemical and Industrial Properties Even Under Non‐Stress Conditions

10.21203/rs.3.rs-361740/v1 ◽

2021 ◽

Author(s):

Nour Chiab ◽

mariam kammoun ◽

mariam charfeddine ◽

rim mzid ◽

radhia gargouri-bouzid

Keyword(s):

Transcription Factor ◽

Transcription Factors ◽

Transgenic Plants ◽

Leaf Shape ◽

Soluble Sugars ◽

Chemical Properties ◽

Transgenic Potato ◽

Tuber Quality ◽

Potato Plants ◽

Positive Effect

Abstract The tryptophan-arginine-lysine-tyrosine (WRKY) transcription factors play important roles in plant growth and response to biotic and abiotic stresses. Several studies described WRKY factors overexpression in different plant species. In a number of cases, modification in morphology and seed size were described in transgenic plants overexpressing transcription factors from this family. In this work, we aimed to study the effect of a grapevine VvWRKY2 transcription factor on transgenic potato plants growth, yields and chemical properties of tubers produced out of any stress condition in the greenhouse and in the field. The results were compared with the wild type commercial variety "Belle de Fontenay" (WT). Data showed that the overexpression of the VvWRKY2 transcription factor increased significantly the leaf area and stem diameter of transgenic plants in comparison to WT. A modification of leaf shape was also noticed in VvWRKY2 overexpressing potato plants. The tuber yield, caliber, dry matter, starch and mineral content of transgenic tubers was higher than WT, while soluble sugars level decreased. All these results suggest that the VvWRKY2 overexpression in potato plants seems to have positive effect on plant vigor, yield and tuber quality under normal conditions in addition to its positive effect on plant response to stresses.

Download Full-text

RESISTANCE OF TRANSGENIC POTATOES TO ATTACK BY EPITRIX CUCUMERIS (COLEOPTERA: CHRYSOMELIDAE)

The Canadian Entomologist ◽

10.4039/ent131423-4 ◽

1999 ◽

Vol 131 (4) ◽

pp. 423-431 ◽

Cited By ~ 5

Author(s):

Jeff G. Stewart ◽

Jennifer Feldman ◽

Debby A. LeBlanc

Keyword(s):

Transgenic Plants ◽

Prince Edward Island ◽

Flea Beetle ◽

Transgenic Potato ◽

Growth Stages ◽

Potato Plants ◽

Transgenic Potato Plants ◽

Transgenic Potatoes ◽

Choice Feeding ◽

Potato Flea Beetle

AbstractPotato plants (Solanum tuberosum, cv. Russet Burbank) genetically altered to produce the CryIIIA protein from Bacillus thuringiensis var tenebrionis were tested for susceptibility to attack by the potato flea beetle, Epitrix cucumeris (Harris), on Prince Edward Island, Canada, from 1993 to 1996. Average weekly damage to the fourth terminal leaf of transgenic potato plants was 31% lower in 1993 and 20% lower in 1994 compared with nontransgenic plants. Transgenic plants had 29% fewer potato flea beetle adults when compared with nontransgenic plants in 1994, although no difference was detected between the two plant types in 1993. Recovery of potato flea beetle pupae in the soil was noticeably lower from transgenic plants expressing the CryIIIA protein in all parts of the plant (Construct No. RBBT02-10Y1) than from either the transgenic plants that expressed the CryIIIA protein primarily in the green foliage (Construct No. RBBT04-01) or the nontransgenic plants. Adult potato flea beetles, when given a choice between transgenic and nontransgenic leaf material, fed preferentially on nontransgenic material. In contrast, when adults were not given a choice, feeding damage was similar between treatments. These results suggest that the reason for reduced feeding on transgenic potato plants by the potato flea beetle is, at least in part, due to some toxicity of the plants to immature growth stages and a preference for adults to feed on nontransgenic material when it is available.

Download Full-text

Obtaining of transgenic potato plants (Solanum tuberosum L.) that contain antisense sequence of prolindehydrogenase gene

Faktori eksperimental'noi evolucii organizmiv ◽

10.7124/feeo.v22.965 ◽

2018 ◽

Vol 22 ◽

pp. 299-304

Author(s):

O. O. Ovcharenko ◽

V. A. Rudas ◽

N. L. Shcherbak ◽

M. V. Kuchuk

Keyword(s):

Solanum Tuberosum ◽

Transgenic Plants ◽

Genetic Transformation ◽

Solanum Tuberosum L ◽

Practical Interest ◽

Transgenic Potato ◽

Proline Dehydrogenase ◽

Potato Plants ◽

Antisense Sequence ◽

Transgenic Potato Plants

Aim. The aim of our work was to obtain transgenic potato plants of Ukrainian varieties with the expression of a double-stranded RNA suppressor of proline dehydrogenase gene. We propose the decrease of proline degradation level and increase of overall proline concentration in obtained transgenic plants. Methods. The Agrobasterium tumefaciens-mediated method of genetic transformation to obtain transgenic plants of potato was used. Internodes of aseptic potato plants were transformed with a binary vector pBi2E containing an inverted repeats of two copies of proline dehydrogenase gene’s first exon and the gene of neomycin phosphotransferase II (nptII). Results. As a result of experiments kanamycin resistant transgenic potato lines of Deseiree, Belarusian 12 and Slavianka varieties were obtained. The transgenic nature of the obtained plants was confirmed by PCR with primers specific to the first exon of proline dehydrogenase and to nptII genes. Conclusions. The optimized conditions of genetic transformation and used agrobacterial strain allow to obtain the transgenic plants of a model potato variety Désirée, as well as varieties Belorussian 12 and Slovyanka which are of practical interest for cultivation in Ukraine. Keywords: transgenic plants, potatoes (Solanum tuberosum L.), stress resistance, proline.

Download Full-text

POTENTIAL OF PROTEINASE INHIBITORS FOR THE PRODUCTION OF TRANSGENIC PLANTS TOLERANT TO INSECT PESTS. I. FUNCTIONAL ASPECTS

HortScience ◽

10.21273/hortsci.29.4.249e ◽

1994 ◽

Vol 29 (4) ◽

pp. 249e-249

Author(s):

Dominique Michaud ◽

Serge Overney ◽

Binh Nguyen-Quoc ◽

Serge Yelle

Keyword(s):

Transgenic Plants ◽

Insect Pests ◽

Proteinase Inhibitors ◽

Transgenic Potato ◽

Control Approach ◽

Potato Plants ◽

Potato Beetle ◽

Functional Aspects ◽

Digestive Proteinases ◽

Transgenic Potato Plants

In the past few years, transformation of plant genomes with proteinase inhibitor (PI) genes has been proposed as an effective way to produce insect-tolerant plants. For such a control approach, however, biochemical studies are necessary to assess the effect of PIs on not only insect digestive proteinases (target enzymes) but also plant endogenous proteinases (nontarget enzymes). As an example, transformation of potato (Solanum tuberosum L.) with oryzacystatin (OC) genes, two cysteine PIs, was considered for controlling Colorado potato beetle (CPB; Leptinotarsa decemlineata Say). The use of electrophoretic approaches and standard assays showed that CPB uses at least 14 cysteine proteinases for protein digestion throughout its development. Proteinases of the same class were also detected in sprouting potato tuber extracts, suggesting a potential interference of cPIs in transgenic plants. While OCs inhibit a significant fraction of CPB digestive proteinases, no inactivation of potato proteinases was detected. This apparent absence of direct interference suggests the real potential of OCs for producing CPB-tolerant transgenic potato plants.

Download Full-text