Overexpression of Solanum tuberosum respiratory burst oxidase homolog A (StRbohA) promotes potato tolerance to Phytophthora infestans.

2021 ◽  
Author(s):  
Atta Soliman ◽  
Lorne R. Adam ◽  
Pawanpuneet K. Rehal ◽  
Fouad Daayf
Keyword(s):  
Solanum Tuberosum ◽  
Transgenic Plants ◽  
Phytophthora Infestans ◽  
Respiratory Burst ◽  
Defense Mechanisms ◽  
Transgenic Potato ◽  
Plant Tolerance ◽  
Potato Plants ◽  
Pathogenesis Related ◽  
Respiratory Burst Oxidase

Reactive oxygen species (ROS) represent one of the first lines of plants’ biochemical defense against pathogens. Plants’ respiratory burst oxidase homologs (RBOHs) produce ROS as by-products in several cellular compartments. In potato tubers, Solanum tuberosum respiratory burst oxidase homolog (StRBOHs) are involved in suberization and healing of wounded tissues. StRbohA has been tested in the model plant Arabidopsis thaliana, which led to enhanced plant defense against the soil-borne pathogen Verticillium dahliae. Here, we showed that overexpressing StRbohA in potato plants enhancesd plant tolerance to the oomycete Phytophthora infestans, the causal agent of late blight disease. Transgenic potato plants expressing StRbohA showed reduced disease symptoms (necrosis) compared to the wild type check. The In parallel, the expression of pathogenesis-related genes (PRs), RBOHs, antioxidation-related genes CPRX1, PRX2, APRX1, CAT1, and CAT2, and genes involved in the biosynthesis pathways of jasmonic and salicylic acids (ICS, PAL1, PAL2, LOX1, LOX2, and LOX3) exhibited significant increases in the transgenic plants in response to infection. Following higher expression of RBOHs, ROS accumulated more in inoculation sites of the transgenic plants. ROS act as signals that activate gene expression in the SA biosynthesis pathway, leading to the accumulation of SA and triggering SA-based defense mechanisms. SA-responsive pathogenesis-related genes (PRs) showed higher expression in the transgenic plants, which resulted in the restriction of pathogen growth in plant tissues. These results represent a demonstration of the effective role of StRbohA in enhancing potato defense against P. infestans.

Transgenic Potato Plants Expressing Soybean ß-1,3-Endoglucanase Gene Exhibit an Increased Resistance to Phytophthora infestans

1998 ◽  
Vol 53 (11-12) ◽  
pp. 1012-1016 ◽  
Author(s):  
Maria Borkowska ◽  
Magdalena Krzymowska ◽  
Andrzej Talarczyk ◽  
Malik F. M. Awan ◽  
Ludmila Yakovleva ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Phytophthora Infestans ◽  
Cell Walls ◽  
Fungal Pathogens ◽  
Pcr Amplification ◽  
Transgenic Potato ◽  
Fungal Cell ◽  
Potato Genome ◽  
Potato Plants ◽  
Transgenic Potato Plants

Abstract Soybean β-1,3-endoglucanase represents a model system for studies on early plant re­sponses to infection by fungal pathogens, and it has been implicated in the release of elicitors from fungal cell walls. In the present study, potato plants were transformed with the soybean β-1,3-endoglucanase cDNA via Agrobacterium delivery system. The transfer of the gene into potato genome was confirmed by (i) PCR amplification, (ii) Northern blot analyses, and (Hi) an increase in the activity of β-1,3-endoglucanase in transgenic plants. The transformation resulted in an increased resistance of selected transgenic plants to infection by Phytophthora infestans, an important pathogen.

scholarly journals Obtaining of transgenic potato plants (Solanum tuberosum L.) that contain antisense sequence of prolindehydrogenase gene

2018 ◽  
Vol 22 ◽  
pp. 299-304
Author(s):  
O. O. Ovcharenko ◽  
V. A. Rudas ◽  
N. L. Shcherbak ◽  
M. V. Kuchuk
Keyword(s):  
Solanum Tuberosum ◽  
Transgenic Plants ◽  
Genetic Transformation ◽  
Solanum Tuberosum L ◽  
Practical Interest ◽  
Transgenic Potato ◽  
Proline Dehydrogenase ◽  
Potato Plants ◽  
Antisense Sequence ◽  
Transgenic Potato Plants

Aim. The aim of our work was to obtain transgenic potato plants of Ukrainian varieties with the expression of a double-stranded RNA suppressor of proline dehydrogenase gene. We propose the decrease of proline degradation level and increase of overall proline concentration in obtained transgenic plants. Methods. The Agrobasterium tumefaciens-mediated method of genetic transformation to obtain transgenic plants of potato was used. Internodes of aseptic potato plants were transformed with a binary vector pBi2E containing an inverted repeats of two copies of proline dehydrogenase gene’s first exon and the gene of neomycin phosphotransferase II (nptII). Results. As a result of experiments kanamycin resistant transgenic potato lines of Deseiree, Belarusian 12 and Slavianka varieties were obtained. The transgenic nature of the obtained plants was confirmed by PCR with primers specific to the first exon of proline dehydrogenase and to nptII genes. Conclusions. The optimized conditions of genetic transformation and used agrobacterial strain allow to obtain the transgenic plants of a model potato variety Désirée, as well as varieties Belorussian 12 and Slovyanka which are of practical interest for cultivation in Ukraine. Keywords: transgenic plants, potatoes (Solanum tuberosum L.), stress resistance, proline.

scholarly journals Alleviation of Phytophthora infestans Mediated Necrotic Stress in the Transgenic Potato (Solanum tuberosum L.) with Enhanced Ascorbic acid Accumulation

Plants ◽  
2019 ◽  
Vol 8 (10) ◽  
pp. 365 ◽  
Author(s):  
Ill-Min Chung ◽  
Baskar Venkidasamy ◽  
Chandrama Prakash Upadhyaya ◽  
Gurusaravanan Packiaraj ◽  
Govindasamy Rajakumar ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Transgenic Plants ◽  
Phytophthora Infestans ◽  
Solanum Tuberosum L ◽  
Transgenic Potato ◽  
Pr Genes ◽  
Pathogen Infection ◽  
Untransformed Control ◽  
Expression Of Genes ◽  
Pathogenesis Related

Potato is the most widely cultivated non-cereal crop in the world, and like any other crop, it is susceptible to yield losses because of various factors, including pathogen attacks. Among the various diseases of potato, late blight caused by the oomycete Phytophthora infestans is considered as the most devastating disease worldwide. In this study, transgenic potato plants overexpressing the D-galacturonic acid reductase (GalUR) gene with an enhanced level of cellular L-ascorbate (L-AsA) were challenged with Phytophthora infestans to determine the level of stress tolerance induced in those plants. With the onset of pathogen infection, necrotic lesions progressively expanded and became necrotic in the control plants. The transgenic potato lines with enhanced ascorbic acid showed reduced necrotic lesions. Hydrogen peroxide (H2O2) and malondialdehyde (MDA) levels were relatively lower in transgenic plants compared to the untransformed control (UT) plants. The mRNA expressions of pathogenesis-related (PR) genes, such as pathogenesis related 1 (PR1) and phenylalanine ammonia-lyase (PAL) were slightly higher in GalUR overexpressing transgenic lines as compared to the untransformed control plants. Pathogen infection also altered the mRNA expression of genes associated with gibberellic acid (GA) and abscisic acid (ABA) biosynthesis. Furthermore, the increase in various antioxidant enzymes was also observed in the gene expression analysis with the transgenic plants. The complete loss of the pathogen growth and disease occurrence was not observed in our study; however, the findings indicated that an increase in the level of cellular L-ascorbate in the transgenic potato leads to enhanced cellular antioxidants, PR genes and plant defense hormones, such as GA and ABA resulting in the reduction of the disease symptoms caused by the Phytophthora infestans.

Localization of Ds-transposon containing T-DNA inserts in the diploid transgenic potato: linkage to the R1 resistance gene against Phytophthora infestans (Mont.) de Bary

Genome ◽  
1996 ◽  
Vol 39 (2) ◽  
pp. 249-257 ◽  
Author(s):  
A. El-Kharbotly ◽  
J. M. E. Jacobs ◽  
B. te Lintel Hekkert ◽  
W. J. Stiekema ◽  
A. Pereira ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Phytophthora Infestans ◽  
Resistance Gene ◽  
Dna Sequences ◽  
Transgenic Potato ◽  
Potato Genome ◽  
Rflp Map ◽  
Polymerase Chain ◽  
R1 Gene ◽  
Map Location

The Dissociation transposable element (Ds) of maize containing NPTII was introduced into the diploid potato (Solanum tuberosum) clone J91-6400-A16 through Agrobacterium tumefaciens mediated transformation. Genomic DNA sequences flanking the T-DNAs from 312 transformants were obtained with inverse polymerase chain reaction or plasmid rescue techniques and used as probes for RFLP linkage analysis. The RFLP map location of 60 T-DNAs carrying Ds–NPTII was determined. The T-DNA distribution per chromosome and the relative distance between them appeared to be random. All 12 chromosomes have been covered with Ds-containing T-DNAs, potentially enabling tagging of any gene in the potato genome. The T-DNA insertions of two transformants, BET92-Ds-A16-259 and BET92-Ds-A16-416, were linked in repulsion to the position of the resistance gene R1 against Phytophthora infestans. After crossing BET92-Ds-A16-416 with a susceptible parent, 4 desired recombinants (Ds carrying T-DNA linked in coupling phase with the R1 gene) were discovered. These will be used for tagging the R1 gene. The efficiency of the pathway from the introduction to localization of T-DNAs is discussed. Key words : Solanum tuberosum, Phytophthora infestans, Ds element, transposon tagging, R genes, euchromatin.

scholarly journals Copper and nickel resistance at ipt-transgenic potato plants

2004 ◽  
Vol 2 (3) ◽  
pp. 25-31
Author(s):  
Elena A Andreeva ◽  
Ludmila A Lutova
Keyword(s):  
Transgenic Plants ◽  
Copper Sulfate ◽  
Transgenic Potato ◽  
Nickel Chloride ◽  
Nickel Resistance ◽  
Potato Plants ◽  
Agrobacterial Transformation ◽  
Enhanced Resistance ◽  
Transgenic Potato Plants

Using agrobacterial transformation collection of /pr-transgenic plants based on cv. Adretta was obtained. Analysis of transgenic plants susceptibility to the exogenous phytohormones auxin (IAA) and cytokinin (kinetin) for 18 from 22 analyzed forms reveals altered reaction to hormones. Among 18 plants with altered reaction to phytohormones for 16 forms enhanced resistance to copper sulfate and/or nickel chloride was observed

Toxicity of free riboflavin and methionine–riboflavin solutions to Phytophthora infestans and the reduction of potato late blight disease

1992 ◽  
Vol 38 (11) ◽  
pp. 1108-1111 ◽  
Author(s):  
C. M. Jordan ◽  
R. J. Wakeman ◽  
J. E. DeVay
Keyword(s):  
Solanum Tuberosum ◽  
Phytophthora Infestans ◽  
Synthetic Medium ◽  
Hyphal Growth ◽  
Fluorescent Light ◽  
Potato Plants ◽  
Contrast Exposure ◽  
Late Blight Disease ◽  
Blight Disease ◽  
Liquid Synthetic Medium

Treatments of sporangia and zoospores of Phytophthora infestans race 1.2.3.4 with methionine or riboflavin for durations of up to 8 h under fluorescent light did not affect its colonization of rye-seed agar. In contrast, exposure of sporangia and zoospores to methionine-riboflavin mixture for 2 h or more resulted in the failure of race 1.2.3.4 to grow when transferred to rye-seed agar medium. Hyphal growth of races 1.2.3.4 and 0, when incubated in liquid synthetic medium, was inhibited by free riboflavin. Potato plants, Solanum tuberosum cv. Kennebec, when pretreated with a methionine–riboflavin mixture and then spray inoculated with race 1.2.3.4 developed fewer diseased leaves per plant than plants pretreated with water, methionine, or riboflavin. Key words: Solanum tuberosum, hydroxyl radical, superoxide anion.

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