ʟ-Ovothiol A: The Egg Release Pheromone of the Marine Polychaete Platynereis Dumerilii: Annelida: Polychaeta

1999 ◽  
Vol 54 (12) ◽  
pp. 1145-1174 ◽  
Author(s):  
Ingo Röhl ◽  
Bernd Schneider ◽  
Bettina Schmidt ◽  
Erich Zeeck
Keyword(s):  
High Performance Liquid Chromatography ◽  
High Performance ◽  
Sex Pheromones ◽  
Marine Invertebrates ◽  
Reversed Phase ◽  
Coelomic Fluid ◽  
Platynereis Dumerilii ◽  
Marine Polychaete ◽  
First Time ◽  
Sexually Mature

Sex pheromones, released with the coelomic fluid by male Platynereis dumerilii initiate egg release in swarming females. The egg release pheromone, isolated from the coelomic fluid of sexually mature males, was identified as ʟ-Ovothiol A, which was found in male marine invertebrates for the first time. Isolation was obtained by reversed-phase high-performance liquid chromatography in the biologically inactive disulfide form.

scholarly journals CUPRAC assay-guided profiling of antioxidant compounds in methanol extract of Lentinus squarrosulus Mont. mycelium

2018 ◽  
Vol 111 (1) ◽  
pp. 101
Author(s):  
Sumaiyah ABDULLAH ◽  
Noorlidah ABDULLAH ◽  
Vikineswary SABARATNAM ◽  
Ken Choy YAP
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Methanol Extract ◽  
Antioxidant Properties ◽  
Reversed Phase ◽  
Antioxidant Compounds ◽  
Ganoderic Acid ◽  
Food Industries ◽  
First Time

<p>A cupric reducing antioxidant capacity (CUPRAC)-guided purification approach was performed on a methanol extract of <em>Lentinus squarrosulus</em> (LsqMeOH) by using reversed phase-high performance liquid chromatography. Using reversed phase-high performance liquid chromatography, three fractions were separated arbitrarily named FR1, FR2 and FR3. Results showed that FR2 exhibited the highest antioxidant activity in CUPRAC assay (A<sub>450</sub>, 0.86) but not significantly different from LsqMeOH (A<sub>450</sub>, 0.84). FR1 and FR3 showed much lower absorbance, with values (A<sub>450</sub>, 0.21) and (A<sub>450</sub>, 0.36) respectively at 1 mg ml<sup>-1</sup>. The most active fraction (F3) was further subjected to LC-MS/MS to obtain its detailed chemical profile. Uridine, ganoderic acid derivative, and flavonoids were the first time being found in <em>L. squarrosulus</em> antioxidative fractions. The present results indicate that the fraction extracts of <em>L. squarrosulus</em> possess antioxidant properties and can be used as free radical inhibitors. Therefore, this research suggested the potentials of <em>L. squarrosulus</em> as a source of antioxidant extract to be used in food industries (functional food).</p>

Auto-oxidation of 5,6,7 ,8-tetrahydroneopterin

Pteridines ◽  
1998 ◽  
Vol 9 (1) ◽  
pp. 26-28
Author(s):  
Toshiyuki Arai ◽  
Hiroko Mori ◽  
Hisanari Ishii ◽  
Toshinori Suzuki ◽  
Shuji Kojima ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Free Radicals ◽  
High Performance ◽  
Oxygen Free Radicals ◽  
Phosphate Buffer Solution ◽  
Reversed Phase ◽  
Buffer Solution ◽  
First Time

Summary Auto-oxidation of 5,6,7,8-tetrahydroneopterin (NPH4 ) in phosphate buffer solution (PBS) was examined using reversed-phase high-performance liquid chromatography. In the chromatograms obtained every 30 min after NPH4 was dissolved in PBS, the peak of NPH4 gradually decreased and almost disappeared 90 min after the dissolution. In contrast, another peak of an unidentified substance (one of dihydroneopterins), appeared from the first time, gradually increased and became most dominant among various peaks 60 min after. The peak of 7,8-dihydroneopterin (7,8-NPH2) appeared 30 min after and gradually increased, but its height was less than that of the above-mentioned peak from first to last. These results indicate that NPH4 is readily oxidized by dissolved oxygen in PBS to produce 7,8-NPH2 and other dihydroneopterins before the reaction with oxygen free radicals and that the substances which actually serve as antioxidants in vivo are these dihydroneopterins.

Development and Validation of A Reversed Phase-High Performance Liquid Chromatography Method for the Quantitative Estimation of Ramipril Drug Content from Formulated Dosage Form

2016 ◽  
Vol 6 (3) ◽  
Author(s):  
Raju Chandra ◽  
Manisha Pant ◽  
Harchan Singh ◽  
Deepak Kumar ◽  
Ashwani Sanghi
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Active Ingredient ◽  
High Performance ◽  
Limit Of Detection ◽  
Quantitative Estimation ◽  
Reversed Phase ◽  
Uv Detector ◽  
Chromatography Method ◽  
Drug Content

A reliable and reproducible reversed-phase high performance liquid chromatography (RP-HPLC) was developed for the quantitative determination of Remipril drug content from marketed bulk tablets. The active ingredient of Remipril separation achieved with C18 column using the methanol water mobile phase in the ratio of 40:60 (v/v). The active ingredient of the drug content quantify with UV detector at 215 nm. The retention time of Remipril is 5.63 min. A good linearity relation (R2=0.999) was obtained between drug concentration and average peak areas. The limit of detection and limit of quantification of the instrument were calculated 0.03 and 0.09 µg/mL, respectively. The accuracy of the method validation was determined 102.72% by recoveries method.

Sign in / Sign up

Export Citation Format

Share Document