Free Radical Scavengers and Antioxidants from Tagetes mendocina

2004 ◽  
Vol 59 (5-6) ◽  
pp. 345-353 ◽  
Author(s):  
Guillermo Schmeda-Hirschmann ◽  
Alejandro Tapia ◽  
Cristina Theoduloz ◽  
Jaime Rodríguez ◽  
Susana López ◽  
...  
Keyword(s):  
Free Radical ◽  
Superoxide Anion ◽  
Radical Scavenging ◽  
Lung Fibroblasts ◽  
Free Radical Scavengers ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
Radical Scavenger ◽  
Radical Scavengers ◽  
Crude Drug

Tagetes mendocina (Asteraceae) is a medicinal plant widely used in the Andean provinces of Argentina. Preliminary assays showed free radical scavenging activity in the methanol extract of the aerial parts, measured by the decoloration of a methanolic solution of the 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) and scavenging of the superoxide anion. Assayguided isolation led to 4′-hydroxyacetophenone (1), protocatechuic acid (2), syringic acid (3), patuletin (4), quercetagetin 7-O-β-d-glucoside (5), patuletin 7-O-β-d-glucoside (6) and axillarin 7-O-β-d-glucoside (7) as the free radical scavengers and antioxidant compounds from Tagetes mendocina. On the basis of dry starting material, the total phenolic content of the crude drug was 3.00% with 0.372% of flavonoids. The content of compounds 1-7 in the crude drug was 0.008, 0.015, 0.010, 0.029, 0.238, 0.058 and 0.017%, respectively. Quercetagetin 7-O-β-d-glucoside proved to be the main free radical scavenger of the extracts measured by the DPPH decoloration test as well as for quenching the superoxide anion and inhibition of lipoperoxidation in erythrocytes. In the lipid peroxidation assay the percentual inhibition was related with the number of methoxy groups in the molecule, ranging from 86% for the quercetagetin glucoside to 67% for the monomethoxylated and 31% for the dimethoxylated derivative. The compounds showed low cytotoxicity towards human lung fibroblasts with IC50 > 1mᴍ for compounds 1-3 and 0.24 to 0.52 mm for the flavonoids 4-7.

scholarly journals PHYTOCHEMICAL SCREENING AND IN VITRO ANTIOXIDANT POTENTIAL OF TRIBULUS TERRESTRIS FRUIT AND MESUA FERREA FLOWER EXTRACTS: A COMPARATIVE STUDY

2018 ◽  
Vol 10 (3) ◽  
pp. 70
Author(s):  
Dakshayini P. N. ◽  
Mahaboob Basha P.
Keyword(s):  
Free Radical ◽  
Radical Scavenging ◽  
Pharmaceutical Products ◽  
Free Radical Scavenging ◽  
Aluminium Chloride ◽  
Free Radical Scavengers ◽  
Total Phenolic ◽  
P Value ◽  
Tribulus Terrestris ◽  
Ethanolic Extracts

Objective: This study aimed to investigate the antioxidants and free radicals scavenging properties of ethanolic extracts from Tribulus terrestris fruit (TTF) and Mesua ferrea flower (MFF).Methods: The antioxidant and free radical scavenging activities were measured by using standard protocols against phytoextracts used at 50, 100, 150, 200 and 250 µg/ml concentration. Total phenolic and flavonoid content were estimated using Folin-Ciocalteu and aluminium-chloride reagent assay methods.Results: Among the extracts, MFF showed the highest total antioxidant ability followed by TTF. With regard to various scavenging activities, MFF had the highest radical scavenging activity than TTF extract closely resembled the standards. Also, the reducing capacity on ferrous ion was high in MFF than TTF. The quantified phenolic contents of TTF and MFF ethanolic extracts were 27.0±0.36 and 57.0±0.32 mg gallic acid equivalent per gram extract respectively. A positive correlation (p value<0.001) was observed between phenolic content and free radical scavenging efficiencies.Conclusion: The results confirm that both MFF and TTF are important sources of natural antioxidants and serves as an effective free radical scavengers and/or inhibitors. Hence, both extracts are suitable plant-based pharmaceutical products can be used to handle 'free radical-mediated diseases.

scholarly journals Biological Activity of the Methanol and Water Extracts of the Fruits of Anthocyanin-Rich Plants Grown in South-West Poland

2015 ◽  
Vol 10 (3) ◽  
pp. 1934578X1501000 ◽  
Author(s):  
Paulina Strugala ◽  
Anna Dudra ◽  
Alicja Z. Kucharska ◽  
Anna Sokół-Łętowska ◽  
Dorota Wojnicz ◽  
...  
Keyword(s):  
Free Radical ◽  
Biological Effects ◽  
Radical Scavenging ◽  
Antibacterial Properties ◽  
Bacterial Biofilm ◽  
Free Radical Scavengers ◽  
Radical Scavengers ◽  
Water Extracts ◽  
South West ◽  
Anti Inflammatory

In this study we focused on the chemical composition, antioxidant, anti-inflammatory and antibacterial properties of methanol and water extracts of fruits of six species grown in south-west Poland: blackberry, blackcurrant, chokeberry, cherry, gooseberry and raspberry. No general correlation could be drawn between extract chemical properties (i.e. antioxidant and free radical scavenging) and observed biological effects. However, blackcurrant and blackberry extracts (both methanol and water) appeared to be good antioxidants and free radical scavengers, as well as effective inhibitors of E. coli adhesion to the uroepithelial cells. On the other hand, the poorest antioxidants and free radical scavengers, cherry and gooseberry extracts, also poorly affected bacterial growth, swimming ability and adhesion to epithelial cells. Surprisingly, gooseberry extracts, which showed generally weak effects, appeared to be the most effective inhibitor of bacterial biofilm formation. Additionally, most of the studied extracts showed reasonably strong anti-inflammatory properties.

scholarly journals AOP1, a New Live Cell Assay for the Direct and Quantitative Measure of Intracellular Antioxidant Effects

Antioxidants ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 471 ◽  
Author(s):  
Camille Gironde ◽  
Mylène Rigal ◽  
Cécile Dufour ◽  
Christophe Furger
Keyword(s):  
Free Radical ◽  
Radical Scavenging ◽  
Epigallocatechin Gallate ◽  
Quantitative Measure ◽  
Cell System ◽  
Butylated Hydroxytoluene ◽  
Free Radical Scavengers ◽  
Live Cells ◽  
Antioxidant Compounds ◽  
Anti Oxidant

Taking advantage of Light Up Cell System (LUCS) technology, which allows for fine monitoring of reactive oxygen species (ROS) production inside live cells, a new assay called Anti Oxidant Power 1 (AOP1) was developed to specifically measure ROS and/or free-radical scavenging effects inside living cells. This method is quantitative and EC50s obtained from AOP1 dose-response experiments were determined in order to classify the intracellular antioxidant efficacy of 15 well known antioxidant compounds with different hydrophilic properties. Six of them (epigallocatechin gallate, quercetin, butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), ethoxyquin, resveratrol) gave EC50s in the range of 7–64 μM, four (Trolox, catechin, epicatechin, EUK134) in the range of 0.14 to 1 mM, and 5 (sulforaphane, astaxanthin, α- and γ-tocopherols, vitamin E acetate) showed only partial or no effect. Interestingly, effects with measurable EC50s were observed for compounds with hydrophilic properties (LogP ≤ 5.3), while all antioxidants known to act at the plasma membrane level (LogP ≥ 10.3) had partial or no effect. Sulforaphane, a hydrophilic but strict Keap1/Nrf2 pathway enhancer, did not show any effect either. Importantly, AOP1 assay captures both antioxidant and prooxidant effects. Taken together, these results led us to the conclusion that AOP1 assay measures antioxidant effect of compounds that selectively enter the cell, and act as free radical scavengers in the cytosol and/or nucleus level.

scholarly journals Extraction, Antioxidant Capacity, 5-Lipoxygenase Inhibition, and Phytochemical Composition of Propolis from Eastern Canada

Molecules ◽  
2020 ◽  
Vol 25 (10) ◽  
pp. 2397
Author(s):  
Mariama Sambou ◽  
Jacques Jean-François ◽  
Fanta J. Ndongou Moutombi ◽  
Jérémie A. Doiron ◽  
Mathieu P.A. Hébert ◽  
...  
Keyword(s):  
Free Radical ◽  
Radical Scavenging ◽  
Solvent Polarity ◽  
Reversed Phase ◽  
Hek293 Cells ◽  
Free Radical Scavengers ◽  
Total Phenolic ◽  
Chromatographic Retention ◽  
Eastern Canada ◽  
Polar Solvents

Soxhlet (SE), microwave-assisted (MAE) and ultrasound-assisted (UAE) extraction were compared using ten extraction solvents for their efficiency to extract phenolic and flavonoid antioxidants from Eastern Canada propolis. Extracts were compared for total phenolic (TPC) and total flavonoid (TFC) content, and radical scavenging activities. Anti-inflammatory activity through inhibition of 5-lipoxygenase (5-LO) products biosynthesis in HEK293 cells was also evaluated. The results showed that SE extracts using polar solvents had the highest TPC and TFC. Extracts obtained with ethanol, methanol and acetone were effective free radical scavengers, and showed 5-LO inhibition similar to zileuton. UAE was an effective extraction method since the extracts obtained were comparable to those using SE and the MAE while being done at room temperature. With UAE, extracts of less polar solvents showed similar free radical scavenging and 5-LO inhibition to extracts of much more polar solvents such as methanol or ethanol. Reversed-phase liquid chromatography tandem mass spectrometry confirmed the presence of 21 natural compounds in the propolis extracts based on the comparison of intact mass, chromatographic retention time and fragmentation patterns derived from commercial analytical standards. The current study is the first of its kind to concurrently investigate solvent polarity as well as extraction techniques of propolis.

scholarly journals Bergenin Content and Free Radical Scavenging Activity of Bergenia Extracts

2015 ◽  
Vol 10 (7) ◽  
pp. 1934578X1501000
Author(s):  
Helena Hendrychová ◽  
Jan Martin ◽  
Lenka Tůmová ◽  
Nina Kočevar-Glavač
Keyword(s):  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Hplc Method ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Free Radical Scavengers ◽  
Radical Scavenger ◽  
Scavenging Activity ◽  
Different Seasons

Our research was focused on the evaluation of bergenin content and free radical scavenging activity of extracts prepared from three different species of Bergenia - B. crassifolia (L.) Fritsch., B. ciliata (Haw.) Sternb. and B. x ornata Stein. collected during different seasons. Using an HPLC method, the highest total amount of bergenin was revealed in the leaves of B. x ornata and B. crassifolia (4.9 - 5.1 mg.g−1). Free radical scavenging power was determined by two methods - FRAP and NADH. The best free radical scavengers were B. crassifolia (FRAP: 6.7 - 15.9 mg GAE.100g−1; NADH: 20.3 - 50.9%) and B. ornata (FRAP: 13.7 - 15.2 mg GAE.100g−1; NADH: 29.3 - 31.1%). The lowest content of bergenin and the weakest radical scavenger was B. ciliata (bergenin: 3.1 mg.g−1; FRAP: 5.5 - 11.0 mg GAE.100g−1; NADH: 23.2 - 25.6%). The presence of a large percentage of bergenin is responsible for the radical scavenging activity, as shown by the results from the FRAP and NADH assays. Significant, positive correlation was found between bergenin content and radical scavenging activity in both methods.

Target Size Analysis by Radiation Inactivation: The Use of Free Radical Scavengers

2005 ◽  
Vol 230 (7) ◽  
pp. 455-463 ◽  
Author(s):  
Gene C. Ness ◽  
Laura C. Pendleton ◽  
Michael J. McCreery
Keyword(s):  
Enzyme Activity ◽  
Free Radical ◽  
Functional Unit ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Target Size ◽  
Free Radical Scavengers ◽  
Radical Scavengers ◽  
Radiation Inactivation ◽  
Glucose 6 Phosphate Dehydrogenase

Several model systems were employed to assess indirect effects that occur in the process of using radiation inactivation analysis to determine protein target sizes. In the absence of free radical scavengers, such as mannitol and benzoic acid, protein functional unit sizes can be drastically overestimated. In the case of glutamate dehydrogenase, inclusion of free radical scavengers reduced the apparent target size from that of a hexamer to that of a trimer based on enzyme activity determinations. For glucose-6-phosphate dehydrogenase, the apparent target size was reduced from a dimer to a monomer. The target sizes for both glutamate dehydrogenase and glucose-6-phosphate dehydrogenase in the presence of free radical scavengers corresponded to subunit sizes when determinations of protein by sodium dodecyl sulfate–polyacrylamide gel electrophoresis or immunoblotting were done rather than enzyme activity. The free radical scavengers appear to compete with proteins for damage by secondary radiation products, since irradiation of these compounds can result in production of inhibitory species. Addition of benzoic acid/mannitol to samples undergoing irradiation was more effective in eliminating secondary damage than were 11 other potential free radical scavenging systems. Addition of a free radical scavenging system enables more accurate functional unit size determinations to be made using radiation inactivation analysis.

Discovery of New N-hydrazinecarbothioamide Indazole Hybrids: As Potential Radical (ABTS and DPPH) Scavengers

2020 ◽  
Vol 17 (9) ◽  
pp. 1177-1185
Author(s):  
Rafaila Rafique ◽  
Arshia ◽  
Kanwal ◽  
Khalid Mohammed Khan ◽  
Sridevi Chigurupati ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Free Radicals ◽  
Free Radical ◽  
Radical Scavenging ◽  
Free Radical Scavengers ◽  
Radical Scavengers ◽  
Synthetic Compounds ◽  
Free Radical Generation ◽  
Radical Generation

Background: Free radicals are the main cause of numerous diseases. Their overproduction needs to be controlled in order to combat several ailments. The current study deals with the discovery of new free radical scavengers. Methods: Substituted N-hydrazinecarbothioamide indazoles 1-18 were evaluated for DPPH and ABTS radical scavenging activities. Results: All synthetic compounds possess good radical DPPH and ABTS scavenging potential in the ranges of IC50 = 2.11 ± 0.17 - 5.3 ± 0.11 μM and IC50 = 2.31 ± 0.06 - 5.5 ± 0.07 μM, respectively, as compared to standard ascorbic acid having IC50 = 2.02 ± 0.11 μM for DPPH and IC50 = 2.1 ± 0.07 μM for ABTS. Conclusion: These compounds could serve as leads for antioxidant activity that have the ability to control free radical generation and ward off free radical-induced disorders.

scholarly journals Studies on free radical scavenging substances from microorganisms. II. Neocarazostatins A, B and C, novel free radical scavengers.

1991 ◽  
Vol 44 (8) ◽  
pp. 903-907 ◽  
Author(s):  
SHINICHIRO KATO ◽  
KAZUTOSHI SHINDO ◽  
YOKO KATAOKA ◽  
YUJI YAMAGISHI ◽  
JUNICHIRO MOCHIZUKI
Keyword(s):  
Free Radical ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavengers ◽  
Radical Scavengers

scholarly journals Aktivitas Antioksidan dari Tepung Pisang Goroho Yang Direndam Dengan Lemon Kalamansi

Jurnal MIPA ◽  
2013 ◽  
Vol 2 (2) ◽  
pp. 105 ◽  
Author(s):  
Etni Togolo ◽  
Edi Suryanto ◽  
Meiske S. Sangi
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Phenolic Compounds ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Free Radical Scavengers ◽  
Total Phenolic ◽  
Radical Scavengers ◽  
Banana Flour

Tujuan penelitian ini adalah untuk menentukan kandungan total fenolik dan aktivitas antioksidan dari pisang goroho yang direndam dengan lemon kalamansi. Pisang goroho segar mula-mula direndam dengan lemon kalamansi dengan kadar 25, 50, 75 dan 100%. Selanjutnya pisang goroho dikeringkan di dalam oven dengan suhu 50-60 oC kemudian digiling dan diayak dengan ayakan 65 mesh. Ekstraksi tepung pisang menggunakan metode maserasi selama 24 jam. Analisis kandungan total fenolik dan penentuan aktivitas penangkal radikal bebas menggunakan metode DPPH (1,1-diphenyl-2-picrylhidrazil). Hasil dari penelitian ini menunjukkan bahwa ekstrak tepung pisang dengan perendaman lemon kalamansi 75% memiliki kandungan total fenolik lebih tinggi dibandingkan dengan ekstrak tepung pisang yang direndam dengan lemon kalamansi 100, 50, 25 dan 0%. Kandungan total fenolik berturut-turut adalah 74,80; 58,18; 45,82; 34,90 dan 20,70 µg/mL. Ekstrak tepung pisang yang direndam dengan lemon kalamansi 100% memiliki aktivitas antioksidan yang tidak berbeda nyata dengan perendaman 50 dan 75% (p>0,05). Hasil penelitian diperoleh bahwa pisang goroho yang direndam dengan lemon kalamansi memiliki kandungan senyawa fenolik dan aktivitas antioksidan.This research was aimed to determine the total phenolic content and antioxidant activity of banana goroho marinated in lemon kalamansi. Fresh banana Goroho was first marinated in 25, 50, 75, and 100 % lemon kalamansi. The product was dried in an oven at 50-60 °C and then was milled and sieved to 65 mesh. Banana flour extraction was conducted through maceration for 24 hours. The total phenolic content was analyzed and the free radical scavengers activity was determined using DPPH (1,1-diphenyl-2-picrylhidrazil). The results of this study showed that extracts of banana flour obtained by soaking in 75% lemon kalamansi had total phenolic content higher than that in 100, 50, 25, and 0% lemon kalamansi. Total phenolic content were 74.80, 58.18, 45.82, 34.90, and 20.70 mg/mL respectively. Extract of banana flour obtained by soaking in 100% lemon kalamansi have antioxidant activity which is not significantly different from that in 50 and 75% (p> 0,05). The research obtained that banana goroho soaked in lemon kalamansi had phenolic compounds and antioxidant activity.

Sign in / Sign up

Export Citation Format

Share Document