A New Flavonol Glycoside Derivative from Leaves of Moldenhawera nutans

2005 ◽  
Vol 60 (1-2) ◽  
pp. 45-49 ◽  
Author(s):  
Ademir E. do Vale ◽  
Jorge M. David ◽  
Hugo N. Brandão ◽  
Juceni P. David
Keyword(s):  
Ethyl Acetate Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Dpph Radical ◽  
H Nmr ◽  
Nmr Data ◽  
Phytochemical Studies ◽  
Dpph Radical Scavenging ◽  
Β Carotene ◽  
C Nmr

The ethyl acetate extract of leaves of Moldenhawera nutans Queiroz & Alkin (Leguminosae) furnished, besides methyl gallate and gallic acid, the flavonols named laricetrin, laricetrin 3-glucoside and laricetrin 3-galactoside as well as the new one named laricetrin 5-galloyl-3- β-ᴅ-xylopyranoside. It also was isolated from the hexane extract: β-sitosterol, lupenone, β- amyrinone, α-amyrinone, lupeol, β-amyrin, α-amyrin and α-tocopherol. The antioxidant activities of flavonoids were measured through DPPH radical scavenging and inhibition of auto-oxidation of β-carotene methods. The structures of the compounds were determined by analyses of spectral data. This is the first report dealing with phytochemical studies of leaves of M. nutans. In addition this current work describes the unequivocal attribution of 1H NMR and 13C NMR data of laricetrin.

Anticholinesterase and Antioxidant Activities of Spilanthes filicaulis Whole Plant Extracts for the Management of Alzheimer’s Disease

2019 ◽  
Vol 15 (2) ◽  
pp. 103-113
Author(s):  
Taiwo O. Elufioye ◽  
Cynthia C. Unachukwu ◽  
Adebola O. Oyedeji
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Dpph Radical ◽  
Flavonoid Content ◽  
Whole Plant ◽  
Butyryl Cholinesterase ◽  
Dpph Radical Scavenging

Background: Spilanthes filicaulis is a tropical herb implicated as a memory enhancer in ethnomedicine. Objective: The study investigated acetyl/butyryl cholinesterase inhibitory and antioxidant activities of different extracts of S. filicaulis whole plant and correlated them to its phytochemical constituents. Methods: The powdered whole plant was successively extracted with n-hexane, ethyl acetate and methanol. Acetyl cholinesterase (AChE) and Butyryl cholinesterase (BuChE) inhibitory activity were evaluated by Ellman colorimetry assay. Antioxidant activity was tested using 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, ferric reducing power and nitric oxide scavenging assays. Total phenolic, flavonoid and tannin were estimated using standard methods. Correlation was determined using Quest Graph™ Regression Calculator. Results: Various extracts exhibited concentration-dependent AChE and BuChE inhibitory activity with ethyl acetate extract being the highest with IC50 of 0.77 μg/mL and 0.92 μg/mL for AChE and BuChE respectively. The ethyl acetate extract also showed the highest reducing power when compared with the other extracts. The methanol extract had slightly higher phenolic and flavonoid content and showed the highest DPPH radical scavenging effect. DPPH scavenging, AChE and BuChE inhibition had high correlation with the total flavonoid content with R2 values of 1.00, 0.800 and 0.992 respectively while nitric oxide scavenging had high correlation with phenolics and tannins with R2 = 0.942 and 0.806 respectively. Conclusion: These results show that the extracts of the whole plant of S. filicaulis possess significant AChE/BuChE inhibitory and antioxidant properties, mostly due to its flavonoid content, suggesting the possible use of the plant in neurodegenerative diseases such as AD.

scholarly journals Different Extraction Solvents and Sample Preparation Methods Affect the Antioxidant Activities and Total Phenol Contents of Theobroma Cacao Powder (P01-016-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Hyoeun Yoo ◽  
Hyun-Sook Kim
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Methanol Solution ◽  
Total Phenol ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Freeze Dried ◽  
Dpph Radical Scavenging

Abstract Objectives Cacao(Theobroma cacao) has been widely consumed since more than 4000 years ago which was regarded as sacred food. Cacao has been considered as a strong antioxidant due to its abundant phenolic and especially flavonoid contents. In this experiment, different extraction solvents and methods were used to analyze whether these factors affect the antioxidant activities and phytochemical compounds of cacao powder extracts. Methods Four different cacao extract samples were examined to identify their anti-oxidative activities; raw cacao powder extracted in 70% methanol solution(RM), raw cacao powder extracted in 80% ethanol solution(RE), freeze dried cacao powder extracted in 70% methanol solution(FM), freeze dried cacao powder extracted in 80% ethanol solution(FE). Total phenolic compound content was calculated based on gallic acid standard curve and total flavonoid content was calculated based on the standard curve of quercetin. ABTS scavenging activity was experimented with ABTS reagent diluted by PBS(pH7.4) to make the O.D. value 0.7 ± 0.02 at 734 nm before using 2.5 mM ABTS. The DPPH radical scavenging activity was assessed by 1 mM DPPH methanol solution. Results The contents of total phenol compounds were 45.4 mg GAE/g(RM), 37.3 mg GAE/g(RE), 50.6 mg GAE/g(FM), and 52.7 mg GAE/g(FE). The contents of total flavonoid compounds were 34.3 mg QE/g(RM), 29.7 mg QE/g(RE), 85.1 mg QE/g(FM), and 89.7 mg QE/g(FE). FM group showed the highest ABTS radical scavenging activity which is a marker of antioxidant activity but the other sample groups represented almost as high ABTS radical scavenging activity as FM group. RM group showed the highest DPPH radical scavenging activity which is also a marker of antioxidant activity. Conclusions The results show that different extract solvents and sample preparation methods affect DPPH radical scavenging activity, total phenol and flavonoid contents of cacao powder extracts. Therefore, appropriate methods should be applied according to the purpose of the study. Funding Sources Department of Food and Nutrition, Sookmyung Women's University, Republic of Korea.

scholarly journals THE CHARACTERISTICS AND ANTIOXIDANT ACTIVITIES OF CHABA MAPLE (HIBISCUS ACETOSELLA) HOMEMADE JAM

2019 ◽  
pp. 52-55
Author(s):  
SURADWADEE THUNGMUNGMEE ◽  
NAKUNTWALAI WISIDSRI ◽  
WARACHATE KHOBJAI ◽  
THISAKORN DUMRONGPHUTTIDECHA ◽  
KHEMJIRA JAMKOM ◽  
...  
Keyword(s):  
High Performance ◽  
Antioxidant Activities ◽  
Sugar Content ◽  
Radical Scavenging ◽  
Chemical Properties ◽  
Chemical Characteristics ◽  
Dpph Radical ◽  
Antioxidant Power ◽  
Radical Scavenging Ability ◽  
Dpph Radical Scavenging

Objective: This study aimed to characterize physicochemical and chemical characteristics of Chaba maple (Hibiscus acetosella) homemade jam (CHJ)and determine its autoxidation ability.Methods: The physicochemical and chemical characteristics of CHJ were investigated. The color, viscosity, and pH were observed as physicochemicaldata while chemical properties were obtained from sugar content and total polyphenol content (TPC), determined using high-performance liquidchromatography refractometer and Folin–Ciocalteu assay, respectively. The antioxidant activities of CHJ were identified using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, ferric reducing antioxidant power (FRAP), and nitric oxide (NO) radical scavenging ability methods.Results: The color and viscosity of CHJ were purple-red and 34,483.33±152.75 cP, respectively. The pH was at 3.78. The total sugar was not detectedin CHJ. The TPC of CHJ showed the highest (47.18±1.80 mg gallic acid equivalent [GAE]/g of jam) followed by Streamline (SL) (23.66±0.32 mgGAE/g of jam), Doikham (DK) (21.99±0.50 mg GAE/g of jam), and Best food (BF) (9.75±0.38 mg GAE/g of jam), respectively. Antioxidant activitiesof CHJ with %1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging of 100.00±1.39% and FRAP value of 1690.70±8.26 uM. Both of activitiesexhibited the highest activity and significantly different when compared with other jams. The %NO scavenging activity of CHJ and SL was 72.43±1.93%and 73.82±1.66%, respectively, which higher than DK and BF.Conclusion: This study shows good in both physicochemical and chemical characteristics of CHJ. The CHJ presents the highest TPC as well asantioxidant activities. Thus, a homemade jam of Chaba maple may be considered as a good source of antioxidants and functional foods.

scholarly journals Radical Scavenging and Antioxidant Activities of Essential Oil Components – An Experimental and Computational Investigation

2015 ◽  
Vol 10 (1) ◽  
pp. 1934578X1501000 ◽  
Author(s):  
Farukh S. Sharopov ◽  
Michael Wink ◽  
William N. Setzer
Keyword(s):  
Essential Oil ◽  
Electronic Properties ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Dpph Radical ◽  
Frap Assay ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power ◽  
Oil Components ◽  
Dpph Radical Scavenging

The antioxidant activities of eighteen different essential oil components have been determined using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging assay, the 2,2 ’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical cation assay, and the ferric reducing antioxidant power (FRAP) assay. The phenolic compounds, carvacrol, thymol, and eugenol, showed the best antioxidant activities, while camphor, menthol, and menthone were the least active. The structural and electronic properties of the essential oil components were assessed using density functional theory (DFT) at the B3LYP/6-311++G** level. Correlations between calculated electronic properties and antioxidant activities were generally poor, but bond-dissociation energies (BDEs) seem to correlate with DPPH radical-scavenging activities, and the ferric reducing antioxidant power (FRAP) assay correlated with vertical ionization potentials calculated at the Hartree-Fock/6-311++G** level.

scholarly journals ANTIOXIDANT ACTIVITIES OF FRACTIONS FROM ETHYL ACETATE EXTRACTS OF GARCINIA FRUTICOSA LAUTERB. LEAVES

2018 ◽  
Vol 10 (1) ◽  
pp. 44 ◽  
Author(s):  
Riza Shabrina ◽  
Berna Elya ◽  
Arikadia Noviani
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Group Identification ◽  
Radical Scavenging ◽  
Dpph Radical ◽  
Frap Assay ◽  
Antioxidant Power ◽  
Dpph Radical Scavenging

Objective: This study aimed to fractionate the antioxidant activity of the ethyl acetate leaf extract and to characterize the most active fractionsaccording to compound groups.Methods: The ethyl acetate extract was fractionated with column chromatography using a gradient elution system. Fractions were first screenedqualitatively for antioxidant activity before active fractions were quantified with respect to in vitro antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and the ferric-reducing antioxidant power (FRAP) assay. The compound groups were identifiedfollowing separation by thin-layer chromatography.Results: Fraction 11 exhibited the greatest DPPH radical-scavenging activity, with an IC50 value of 6.58 μg/mL, while the fraction with the greatestantioxidant activity according to the FRAP assay was fraction 10, with a ferric ion equivalent antioxidant activity value of 1015.34 μmol/g.Conclusion: Compound group identification revealed that Fractions 10 and 11 contained flavonoids, with two common to both fractions, whilefraction 10 also contained one specific flavonoid.

STUDY OF ANTIBACTERIAL AND ANTIOXIDANT ACTIVITIES OF SAXICOLOUS LICHEN $ \textit{XANTHOPARMELIA STENOPHYLLA} $

2020 ◽  
Vol 54 (2 (252)) ◽  
pp. 132-137
Author(s):  
A.G. Simonyan ◽  
R.R. Sargsyan ◽  
H.H. Panosyan ◽  
A.H. Trchounian
Keyword(s):  
Methanol Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Gram Positive Bacteria ◽  
Acetone Extracts ◽  
Dpph Radical Scavenging

Antibacterial and antioxidant activities of different extracts of saxicolous lichen Xanthoparmelia stenophylla sampled from Norashen, Gegharkunik Province of Armenia were studied. Methanol, ethanol and acetone extracts of lichen thalli were demonstrated to have activity against only tested gram-positive bacteria. Methanol extract of the lichen showed the highest amount of DPPH radical scavenging activity (~68%). Our studies did not reveal any significant antibacterial and antioxidant activities of aqueous extract.

Chemical Composition of Essential Oil and Antioxidant Activities of Curcuma petiolata Roxb. Rhizomes

2012 ◽  
Vol 506 ◽  
pp. 393-396 ◽  
Author(s):  
A. Thakam ◽  
N. Saewan
Keyword(s):  
Ascorbic Acid ◽  
Essential Oil ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Etoac Extract ◽  
Dpph Radical Scavenging

Pulverized rhizome of Curcuma peliolata on hydrodistillation, afforded light yellow essential oil in the yield of 0.13% v/w. The chemical constituent of the essential oil was determined by GC-MS. Nine compounds representing 95.34 % of the total oil were identified. The major compounds of the oil were 2-methyl-5-pentanol, 1H-pyrrol-1-amine,2-(4-methoxyphenyl)-n,n,5-trimethyl, and curcumol. The distillated aqueous was extracted with dicloromethane (DCM), ethyl acetate (EtOAc), and n-butanol. The DPPH radical scavenging and reducing power of the extracts were investigated. Curcumin and ascorbic acid were used as standard references. The DPPH radical scavenging activity was decrease in order: curcumin ascorbic acid EtOAc extract > DCM extract > n-butanol extract > aqueous extract. The EtOAc extract showed significantly highest inhibition of the radical scavenging activity with IC50 0.092 ± 0.001 mg/mL (curcumin and ascorbic acid (IC50 0.012 and 0.013 mg/mL, respectively (P<0.05)). While, the DCM extract showed highest reducing power with IC50 = 0.271 mg/mL which lower than that of standard curcumin (IC50 = 0.013 mg/mL) and ascorbic acid (IC50 = 0.002 mg/mL).Introduction

scholarly journals Comparison of Depolymerization of Chitosan into Chitooligosaccharides from the Shrimp Shell Waste of Parapeneopsis Stylifera by Non-Specific Enzymes

2021 ◽  
Author(s):  
V. Renuka ◽  
Chandragiri Nagaraja Rao Ravishankar ◽  
Elavarasan Krishnamoorthy ◽  
Abubacker Aliyamveetil Zynudheen
Keyword(s):  
Thermal Behavior ◽  
Antioxidant Activities ◽  
Proteolytic Enzymes ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Structural Difference ◽  
Dpph Radical ◽  
Dpph Radical Scavenging

Abstract Two different non-specific proteolytic enzymes (papain and pepsin) and two carbohydrase enzymes (α-amylase and β-amylase) were used for the depolymerization of chitosan to produce chitooligosaccharides (COS). The COS produced using papain, pepsin, α-amylase and β-amylase were characterized for physico-chemical, structural, thermal and antioxidant activities. Chitooligosaccharides produced using pepsin enzyme had higher solubility compared to other three COS. Structural variation of chitosan and their oligosaccharides were studied by FTIR analysis and it revealed the existence of various structural difference among the oligosaccharides and chitosan. Thermal behavior of chitosan and oligosaccharides were examined using DSC analysis, and it exhibits difference in the glass transition temperature among the four oligosaccharides and native chitosan. Anti-oxidant analysis such as DPPH radical scavenging activity and reducing power revealed the antioxidant ability of chitooligosaccharides. Among the four COS, pepsin-COS had the highest DPPH radical scavenging and reducing power activity. According to the patterns of molecular weight reduction, antioxidant properties, thermal behavior properties, four enzymes α- amylase, β-amylase, pepsin and papain found suitable for hydrolyzing chitosan into chitooligosaccharide. As it is superior to chitosan, chitooligosaccharide can be utilized in food industry as bio-preservatives to enhance the quality, safety and shelf life of the products.

scholarly journals Antioxidant activity of some natural essential oils in Vietnam: Comparison between QSAR simulation and experimental study

2020 ◽  
Vol 129 (1D) ◽  
Author(s):  
Tran Thi Ai My ◽  
Le Trung Hieu ◽  
Nguyen Thi Thanh Hai ◽  
Ton Nu My Phuong ◽  
Huynh Thi Phuong Loan ◽  
...  
Keyword(s):  
Essential Oils ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Qsar Model ◽  
Piper Betle ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Ageratum Conyzoides ◽  
Dpph Radical Scavenging

Antioxidant activities of essential oils from leaves of Piper betle L. (T) and Cleistocalyx operculatus L. (V), and aerial parts of Ageratum conyzoides L. (H) natively grown in Thua Thien Hue province of Vietnam were investigated.Quantitative structure–activity relationship (QSAR) model was constructed from the 27 compounds including 4-hydroxy-chromene-2H-one and its derivatives. DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity was applied to estimate the antioxidant property of these essential oils through IC50 (mg/mL) values. QSAR model is used to predict the radical scavenging activity IC50 mg/mL of T, V, and H. The results indicate that there is a good agreement between the experimental data and the predicted values using the QSAR model. The three essential oils display the DPPH radical scavenging activities with the IC50 values being in the order of T > H > V of 3.71 mg/mL,596.44 mg/mL and 637.03 mg/mL, respectively. The essential oil of T exhibits the strong DPPH radical scavenging activity that is close to the reference compound ascorbic acid (IC50 value of 3.03 mg /mL).

scholarly journals Effects of Solvents on Total Phenolic Content and Antioxidant Activity of Ginger Extracts

2020 ◽  
Author(s):  
Dessie Ezez ◽  
Molla Tefera
Keyword(s):  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Acetone Extract ◽  
Total Phenolic ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Dpph Radical Scavenging

Abstract Ginger (Zingiber officinale) is a popular spice which used for the treatment of different gastrointestinal and inflammatory discomfort. In the present study, the total phenolic content (TPC) and antioxidant activity of ginger extract using four solvents (ethanol, methanol, acetone and ethyl acetate) were determined. Among the four solvents, methanol extract showed that the maximum phenolic (1183.813 mg GAE/100 g at Ayikel and 1022.409 mg GAE/100 g at Mandura) and the least were found in acetone extract (748.865 mg GAE/100 g at Ayikel and 690.152 mg GAE/100 g at Mandura). In addition, the highest DPPH radical scavenging activity (84.868% at Ayikel and 82.883% at Mandura) was observed in methanol. However, acetone showed the least DPPH radical scavenging activity (73.864% at Ayikel and 70.597% at Mandura). Antioxidant activities of ginger extracts were also expressed as IC50 values and acetone extract has maximum IC50 value (0.654 and 0.812 mg/mL) followed by ethyl acetate and ethanol, while the lowest for methanol extracts (0.481 and 0.525 mg/mL). The result of this study showed that extraction solvents significantly affected the total phenolic content and antioxidant activities of ginger. Thus, ginger can be regarded as promising candidates for natural sources of antioxidants with high value of phenolic contents.

Sign in / Sign up

Export Citation Format

Share Document