scholarly journals Gas chromatography coupled to mass spectrometry (GC-MS) characterization and evaluation of antibacterial bioactivities of the essential oils from Piper arboreum Aubl., Piper aduncum L. e Piper gaudichaudianum Kunth

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Ana Cristina A. da Silva ◽  
Edinardo F. F. Matias ◽  
Janaína E. Rocha ◽  
Ana Carolina Justino de Araújo ◽  
Thiago S. de Freitas ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Essential Oils ◽  
Inhibitory Effect ◽  
Antibiotic Activity ◽  
Chemical Components ◽  
Synergistic Activity ◽  
Significant Activity ◽  
Piper Aduncum ◽  
Piper Gaudichaudianum

AbstractThe objective of this study was to determine the chemical profile and to evaluate the antibacterial activity of the essential oils of Piper species and modulation of the antibiotic activity, using the microdilution method to determine the minimum inhibitory concentration. The chemical components were characterized by gas chromatography coupled to mass spectrometry, which revealed β-copaen-4-α-ol (31.38%), spathulenol (25.92%), and germacrene B (21.53%) as major constituents of the essential oils of Piper arboreum, Piper aduncum, and Piper gaudichaudianum, respectively. The essential oils analyzed in this study did not present a clinically relevant activity against standard and multiresistant Escherichia coli. However, in the case of multiresistant Staphylococcus aureus, there was a significant activity, corroborating with reports in the literature, where Gram-positive bacteria are more susceptible to antimicrobial activity. The essential oils modulated the effect of the antibiotics norfloxacin and gentamicin, having on the latter greater modulating effect; however, for erythromycin, no statistically significant effect was observed. In conclusion, the results obtained in this study demonstrated that the essential oils of the analyzed Piper species present an inhibitory effect against S. aureus and modulate antibiotic activity, most of which presents synergistic activity.

scholarly journals Fumigation and repellency of essential oils against Callosobruchus maculatus (Coleoptera: Chrysomelidae: Bruchinae) in cowpea

2017 ◽  
Vol 52 (1) ◽  
pp. 10-17 ◽  
Author(s):  
José Vargas de Oliveira ◽  
Solange Maria de França ◽  
Douglas Rafael e Silva Barbosa ◽  
Kamilla de Andrade Dutra ◽  
Alice Maria Nascimento de Araujo ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Essential Oils ◽  
Callosobruchus Maculatus ◽  
Ricinus Communis ◽  
Syzygium Aromaticum ◽  
Cymbopogon Citratus ◽  
Cinnamomum Zeylanicum ◽  
Main Component ◽  
Piper Aduncum

Abstract: The objective of this work was to assess the fumigant and repellent effects of essential oils on adults of Callosobruchus maculatus and to identify the chemical composition of two of the tested essential oils. For the fumigation test, the oils of Schinus terebinthifolius, Piper aduncum, Syzygium aromaticum, Piper hispidinervum, Cymbopogon citratus, Cinnamomum zeylanicum, and the eugenol compound were tested at different concentrations on C. maculatus adults. For the repellency test, the oils of S. terebinthifolius, P. aduncum, P. hispidinervum, S. aromaticum, Jatropha curcas, and Ricinus communis were evaluated. In the fumigation test, it was observed that P. aduncum and eugenol showed the highest and lowest LC50s, of 169.50 and 0.28 μL L-1 air, respectively. In the repellency test, the oils of S. aromaticum and P. hispidinervum were repellent to C. maculatus. Gas chromatography coupled to mass spectrometry (GC-MS) analysis of these two oils identified 42 compounds, of which safrole was the main component of P. hispidinervum and eugenol of S. aromaticum. The essential oils of S. aromaticum, C. zeylanicum, and the eugenol compound are the most promising to control C. maculatus, via fumigation.

scholarly journals Chemical Composition of the Essential Oils From Twigs, Leaves, and Cones of Thuja plicata and Its Cultivar Varieties “Fastigiata”, “Kornik,” and “Zebrina”

2019 ◽  
Vol 14 (7) ◽  
pp. 1934578X1986290
Author(s):  
Anna Lis ◽  
Agata Swaczyna ◽  
Agnieszka Krajewska ◽  
Karolina Mellor
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Chemical Composition ◽  
Essential Oils ◽  
Thuja Plicata ◽  
Different Parts

The essential oils from different parts of Thuja plicata and its cultivar varieties “Fastigiata”, “Kornik,” and “Zebrina” were analyzed by gas chromatography (GC) and GC/mass spectrometry. More than 80 compounds were identified. The oils from leaves, twigs with leaves, and twigs without leaves contained mainly α-thujone (52.1%-59.2%), fenchone (10.0%-11.3%), and beyerene (3.7%-9.5%), whereas in the cone oil there were α-thujone (35.6%), sabinene (24.0%), and α-pinene (8.3%). The main constituents of the oils from twigs with leaves of “Fastigiata,” “Zebrina,” and “Kornik” cultivars were α-thujone (76.2%, 72.5%, and 67.4%, respectively) and β-thujone (7.6%, 6.2%, and 4.9%, respectively). The oils from cultivars contained more thujones and less fenchone and diterpenes in comparison with T. plicata oil.

scholarly journals Chemical Composition of the Essential Oils from the Flower, Leaf and Stem of Lonicera japonica

2012 ◽  
Vol 7 (5) ◽  
pp. 1934578X1200700
Author(s):  
Nenad Vukovic ◽  
Miroslava Kacaniova ◽  
Lukas Hleba ◽  
Slobodan Sukdolak
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Lonicera Japonica ◽  
Hexadecanoic Acid ◽  
Plant Parts ◽  
Aerial Parts ◽  
Monoterpene Hydrocarbons

The essential oils from different aerial parts of Lonicera japonica have been extracted by hydro-distillation and analyzed by gas chromatography and gas chromatography coupled with mass spectrometry. Quantitative and qualitative differences were found between the analyzed plant parts. A total of eighty-nine compounds were identified. The main constituents were ( Z, Z)-farnesole (16.2%) and linalool (11.0%) for the flowers fraction, hexadecanoic acid (16.0%) and linalool (8.7%) for the leaves fraction, and hexadecanoic acid (31.4%) for the stems. Monoterpene hydrocarbons were absent from all the oils, and oxygenated sesquiterpenes were not identified in the essential oil of the stem.

scholarly journals Suppression of Linalool Acetate Production in Lavandula x intermedia

2009 ◽  
Vol 4 (11) ◽  
pp. 1934578X0900401 ◽  
Author(s):  
Amy Desautels ◽  
Kamal Biswas ◽  
Alexander Lane ◽  
Astrid Boeckelmann ◽  
Soheil S. Mahmoud
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Essential Oil ◽  
Essential Oils ◽  
Gas Chromatography Mass Spectrometry ◽  
Major Constituent ◽  
Improved Method ◽  
Acetate Production ◽  
Essential Oil Production ◽  
Sensitive Method

Linalool acetate, one of the major constituent of several essential oils, is heat-labile and decomposes upon exposure to the high injector temperature during gas chromatography. Here we report the development of an improved method for detection of this compound by gas chromatography mass spectrometry (GCMS) using cold on-column injection of the sample. By using this sensitive method, it has been demonstrated that a lavandin (L.x intermedia) mutant accumulates trace quantities of linalool acetate and camphor and higher amounts of cineole and borneol compared to its parent. This plant, which very likely carries a point mutation in one or more of the genes involved in essential oil production, provides a unique tool for investigating regulation of essential oil biogenesis in plants.

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