Immunohistochemical Assessment of Mast Cells and Small Blood Vessels in Dentigerous Cyst, Odontogenic Keratocyst, and Periapical Cyst

Introdução: Os ceratocistos odontogênicos (CCA) são considerados raros cistos de desenvolvimento, derivados dos remanescentes da lâmina dentária, com atividade intraóssea benigna, porém localmente invasivo e agressivo. O tratamento para o ceratocisto odongênico é variado, podendo-se encontrar modalidades tais como:enucleação, isolada ou associada a curetagem, com osteotomia periférica, aplicação da solução de Carnoy ou crioterapia, descompressão, marsupialização e ressecções. Objetivo: O presente trabalho tem como objetivo relatar um caso de ceratocisto odontogênico, onde foi escolhida abordagem conservadora por curetagem e osteotomia periférica. Relato de caso: Paciente de 68 anos, leucoderma, referiu ao exame clínico dor espontânea em região retromolar esquerda e parestesia em lábio inferior. A paciente foi submetida a biopsia por aspiração e excisional, após confirmação histopatológica foi proposto uma enucleação associada a osteotomia periférica sob anestesia geral. A paciente permanece em acompanhamento clínico e radiográfico, sem sinais de recidiva da lesão. Conclusão: Embora apresentem um comportamento agressivo, os ceratocistos odontogêncios podem ser tratados com segurança, de forma conservadora, por meio de enucleação seguida de osteotomia periférica com mínimo de morbidade. Descritores: Osteotomia; Curetagem; Cistos Odontogênicos. Referências Borghesi A, Nardi C, Giannitto C, Tironi A, Maroldi R, Di Bartolomeo F, Preda L. Odontogenic keratocyst: imaging features of a benign lesion with an aggressive behaviour. Insights Imaging. 2018 Oct;9(5):883-897. Park JH, Kwak EJ, You KS, Jung YS, Jung HD. Volume change pattern of decompression of mandibular odontogenic keratocyst. Maxillofac Plast Reconstr Surg. 2019 Jan 7;41(1):2. Karaca C, Dere KA, Er N, Aktas A, Tosun E, Koseoglu OT, Usubutun A. Recurrence rate of odontogenic keratocyst treated by enucleation and peripheral ostectomy: Retrospective case series with up to 12 years of follow-up. Med Oral Patol Oral Cir Bucal. 2018 Jul 1;23(4):e443-e448. Guerra LAP, Silva PS, Dos Santos RLO, Silva AMF, Albuquerque DP. Tratamento conservador de múltiplos tumores odontogênicos ceratocístico em paciente não sindrômico. Rev cir traumatol. buco-maxilo-fac. 2013; 13(2):43-50. Sundaragiri KS, Saxena S, Sankhla B, Bhargava A. Non syndromic synchronous multiple odontogenic keratocysts in a western Indian population: A series of four cases. J Clin Exp Dent. 2018;10(8):e831-6. Freitas AD, Veloso DA, Santos ALF, Freitas VA. Maxillary odontogenic keratocyst: a clinical case report. RGO Rev Gaúch Odontol. 2015; 63(4):484-88. Madhireddy MR, Prakash AJ, Mahanthi V, Chalapathi KV. Large Follicular Odontogenic Keratocyst affecting Maxillary Sinus mimicking Dentigerous Cyst in an 8-year-old Boy: A Case Report and Review. Int J Clin Pediatr Dent. 2018 Jul-Aug;11(4):349-351. Moura BS, Cavalcante MA, Hespanhol W. Tumor odontogênico ceratocistico. Rev Col Bras Cir., 2016;43(6):466-71. Valori FP, Costa E, Buscatti MY, Oliveira JX, Costa C. Tumor odontogênico queratocístico: características intrínsecas e elucidação da nova nomenclatura do queratocisto odontogênico. J Health Sci Inst. 2010;28(1):80-3. Slusarenko da Silva Y, Stoelinga PJW, Naclério-Homem MDG. The presentation of odontogenic keratocysts in the jaws with an emphasis on the tooth-bearing area: a systematic review and meta-analysis. Oral Maxillofac Surg. 2019;23(2):133-47.

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Expression of inducible nitric oxide synthase in the epithelial linings of odontogenic keratocyst, dentigerous cyst and radicular cyst: A pathological insight

Annals of Medical and Health Sciences Research ◽

10.4103/2141-9248.139330 ◽

2014 ◽

Vol 4 (4) ◽

pp. 583 ◽

Cited By ~ 3

Author(s):

P Swetha ◽

KSV Ramesh ◽

N Madhavan ◽

V Veeravarmal ◽

ASS Sameera

Keyword(s):

Nitric Oxide ◽

Nitric Oxide Synthase ◽

Inducible Nitric Oxide Synthase ◽

Dentigerous Cyst ◽

Odontogenic Keratocyst ◽

Radicular Cyst ◽

Oxide Synthase ◽

Inducible Nitric Oxide

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Analysis of the presence and location of mast cells in periapical cysts and periapical granulomas

RGO - Revista Gaúcha de Odontologia ◽

10.1590/1981-863720160003000023113 ◽

2016 ◽

Vol 64 (4) ◽

pp. 376-381 ◽

Cited By ~ 1

Author(s):

Emerson Filipe de Carvalho NOGUEIRA ◽

Elder Gyress Feitosa FARIAS ◽

Luciano Barreto SILVA ◽

Alexandrino Pereira dos SANTOS NETO ◽

Emanuel Sávio de Souza ANDRADE ◽

...

Keyword(s):

Mast Cells ◽

Granulation Tissue ◽

Blue Staining ◽

Double Blind Study ◽

Double Blind ◽

Cross Sectional ◽

Periapical Granuloma ◽

Staining Methods ◽

Periapical Cyst ◽

Quantitative Descriptive

ABSTRACT Objective: The aim of the present study was to locate mast cells in chronic periapical lesions (granulomas and cysts) by using histochemical techniques and toluidine blue staining. Methods: A quantitative, descriptive, cross-sectional and retrospective research was performed. The sample was obtained from histopathological reports in the archives of the laboratory of surgical pathology of the University of Pernambuco between November 2014 and May 2015. Results: Sixteen cases of granuloma and 21 cases of periapical cysts were selected. The stained slides were analyzed by two examiners at different times, in a double-blind study. Mast cells were found in 13 (61.9%) of the periapical cyst cases, located in the capsule of the lesion. In the periapical granuloma cases, mast cells were found in eight cases (50%), located in the granulation tissue. Conclusion: Mast cells were detected in both cysts and periapical granuloma, located in the capsule and granulation tissue, respectively. Mast cells were more prevalent in periapical cysts than in periapical granuloma.

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The Possible Role of Mast Cells in the Odontogenic Cyst’s Pathogenesis: A Comparative Study between Dentigerous Cyst and Keratocystic Odontogenic Tumor

Pathology Research International ◽

10.1155/2016/8754567 ◽

2016 ◽

Vol 2016 ◽

pp. 1-4 ◽

Cited By ~ 2

Author(s):

Sareh Farhadi ◽

Fatemeh Shahsavari ◽

MirMahdi Davardan

Keyword(s):

Mast Cell ◽

Mast Cells ◽

Dentigerous Cyst ◽

Test Method ◽

Odontogenic Tumor ◽

Blue Staining ◽

Keratocystic Odontogenic Tumor ◽

Wide Range ◽

Significant Difference ◽

Cell Densities

Background. Recently, mast cells were recognized in the pathogenesis of more aggressive pathologic lesions. This study was aimed to evaluate and compare the density of mast cells in Dentigerous cyst (DC) and Keratocystic odontogenic tumor (KCOT) regarding their different clinical behavior. Method. This study was conducted on 23 and 26 cases of DC and KCOT, respectively. Four-micron sections were prepared for Toluidine blue staining and mast cell densities in two desired cysts were studied. Final data was analyzed via t-test and Mann-Whitney U test method regarding the significant level lower than 0.05. Results. Mast cell densities were significantly higher in KCOTs for deep and superficial layers and both layers (P<0.05). The density of degranulated mast cells in the deeper layers and both layers was significantly higher in KCOTs (P<0.05). However, the density of degranulated mast cells in the superficial layer had no significant difference (P>0.05). Conclusion. It seems that mast cells may be involved in the pathogenesis of KCOT, but, regarding wide range of mast cell’s biologic activities, further investigations are recommended to confirm the issue and prepare the details.

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Correlations Between the Density of Tryptase Positive Mast Cells (DMCT) and that of New Blood Vessels (CD105+) in Patients with Gastric Cancer

Romanian Journal of Internal Medicine ◽

10.1515/rjim-2016-0016 ◽

2016 ◽

Vol 54 (2) ◽

pp. 113-120 ◽

Cited By ~ 4

Author(s):

Gianina Viorica Micu ◽

Florica Stăniceanu ◽

Liana Cătălina Sticlaru ◽

Cristiana Gabriela Popp ◽

Alexandra Eugenia Bastian ◽

...

Keyword(s):

Gastric Cancer ◽

Helicobacter Pylori ◽

Mast Cells ◽

Blood Vessels ◽

Malignant Tumors ◽

Helicobacter Pylori Infection ◽

Tumor Spread ◽

Mast Cell Density ◽

Gastric Neoplasia ◽

Distant Tumor

Abstract Mast cells proteases, tryptase and chymase are directly involved in the growth and progression of solid tumors due to their important role in tumor angiogenesis. We examined the density of tryptase positive mast cells and the mean density of new blood vessels in gastric malignant tumors of patients with and without Helicobacter pylori infection, using immunohistochemical staining for tryptase (for mast cells) and CD 105 (for new vessels). Tryptase and CD 105 expression was detected in gastrectomy specimens. In this study, mast cell density correlates with angiogenesis and the growth and progression of gastric cancer. It also shows that the participation of Helicobacter pylori infection in the growth and progress of gastric neoplasia is due to an increase of peritumoral angiogenesis, with subsequent local and distant tumor spread and perivascular growth, but without perineural and nodal involvement.

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Histomorphometric Comparative Study of Blood Vessels and Their Pattern in Follicular Cyst, Odontogenic Keratocyst, and Ameloblastoma

Journal of Craniofacial Surgery ◽

10.1097/scs.0b013e31828014cf ◽

2013 ◽

Vol 24 (2) ◽

pp. 439-445 ◽

Cited By ~ 2

Author(s):

Safora Seifi ◽

Farideh Feizi ◽

Thoraya Khafri ◽

Mehrdad Aram

Keyword(s):

Comparative Study ◽

Blood Vessels ◽

Odontogenic Keratocyst ◽

Follicular Cyst

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Mast cells degranulation affects angiogenesis in the rat uterine cervix during pregnancy

Reproduction ◽

10.1530/rep.1.00018 ◽

2004 ◽

Vol 127 (3) ◽

pp. 379-387 ◽

Cited By ~ 25

Author(s):

J Varayoud ◽

J G Ramos ◽

V L Bosquiazzo ◽

M Muñoz-de-Toro ◽

E H Luque

Keyword(s):

Mast Cell ◽

Mast Cells ◽

Blood Vessels ◽

Uterine Cervix ◽

Vascular System ◽

Mast Cell Degranulation ◽

Endothelial Cell Proliferation ◽

Disodium Cromoglycate ◽

Mast Cell Stabilizer ◽

Vascular Area

During pregnancy, it is essential that sufficient nutrients are supplied by the vascular system to support the dramatic modifications of the rat uterine cervix. Angiogenesis refers to the growth of new blood vessels from pre-existing microcirculation and mast cells have been associated with this process. This study examined the modifications of the vascular compartment and the distribution of mast cells on cervical tissue during pregnancy. Using disodium cromoglycate as a mast cell stabilizer, we determined the effects of the mast cell degranulation on cervical angiogenesis. Mast cell distribution and their degranulation status were evaluated by immunohistochemistry. Endothelial cell proliferation was measured by bromodeoxyuridine incorporation. Vascular areas (absolute and relative) and maturation indices were assessed by quantitative immunohistochemistry of von Willebrand factor and α-smooth muscle actin respectively. Mast cells were predominantly observed during the first half of pregnancy in the perivascular zones. The values of bromodeoxyuridine incorporation, absolute vascular area and vascular maturation index exhibited a significant increase throughout pregnancy. All animals that received mast cell stabilizer showed more than 40% of non-degranulated mast cells. Treated rats exhibited a decrease in endothelial proliferation and in relative vascular area; in addition, a large proportion of mature blood vessels was observed, suggesting a diminished level of new vessel formation. The effects of the mast cell stabilizer were sustained beyond the end of treatment. This is the first report that brings evidence that mast cell degranulation could be a necessary process to contribute to the normal angiogenesis of the rat cervix during pregnancy. Further investigations are needed to elucidate the possible implications of abnormal vascular development of the uterine cervix on the physiological process of ripening and parturition.

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