scholarly journals Bioremediation of MP-polluted Waters Using Bacteria Bacillus licheniformis, Lysinibacillus massiliensis, and Mixed Culture of Bacillus sp. and Delftia acidovorans

2021 ◽  
Author(s):  
Dajana Kučić Grgić ◽  
Martina Miloloža ◽  
Ema Lovrinčić ◽  
Antonija Kovačević ◽  
Matija Cvetnić ◽  
...  
Keyword(s):  
Mixed Culture ◽  
Bacillus Licheniformis ◽  
Bacillus Sp ◽  
Delftia Acidovorans ◽  
Polluted Waters

scholarly journals Overexpression of Thermophilic α-amylase in Bacillus Licheniformis using a High Efficiency Chromosomal Integration and Amplification Strategy

2021 ◽  
Author(s):  
Peili Shen ◽  
Dandan Niu ◽  
Xuelian Liu ◽  
Kangming Tian ◽  
Permaul Kugenthiren ◽  
...  
Keyword(s):  
Bacillus Licheniformis ◽  
High Efficiency ◽  
High Yield ◽  
Chromosomal Integration ◽  
Bacillus Sp ◽  
Strain Development ◽  
Microbial Cell Factories ◽  
Cell Factories ◽  
New Strategy ◽  
Amplification Strategy

Abstract Highly efficient preparation of industrially important enzymes depends on development of the genetically stable and high-yield microbial cell factories, which is often a challengeable laboratory hard work. In aims to simplify strain development with high efficiency for enzyme overproduction, a new strategy based on chromosomal integration and amplification in Bacillus sp . was developed. A pair of plasmids, an integrated expression plasmid pUB'-Ex1 and a thermosensitive replicable plasmid pUB-MazF, were constructed. pUB'-Ex1 conditionally self-replicated in Bacillus sp . when the RepF in pUB-MazF expressed. pUB-MazF thermosensitively self-replicated in Bacillus sp . , which was easily cured from the host by inducing MazF expression with IPTG. Bacillus licheniformis BL-UBM that integrated with pUB-MazF was then transformed with pUB'-amyS derived from pUB'-Ex1 by in-frame cloning of amyS encoding a thermophilic α-amylase from Geobacillus stearothermophilus ATCC 31195. The transformant of B. licheniformis BL-UBM with pUB'-amyS was cultivated at 42 o C with the existence of 1 mmol/l IPTG and 500 μg/ml kanamycin and the recombinants with high α-amylase activities were selected. All tested recombinants were extremely high genetic stability. One of which, recombinant BLiS-002, carried five copies of amyS and produced the highest yield of α-amylase. It could yield 50,753 U/ml of α-amylase in a 50-l bioreactor. The strategy developed in this study is of application potential for convenient and quick strain development for industrially important enzyme overexpression.

scholarly journals Fermentation Characteristics of Soybean Yogurt by Mixed Culture of Bacillus sp. and Lactic Acid Bacteria

2013 ◽  
Vol 26 (2) ◽  
pp. 273-279 ◽  
Author(s):  
Ming Yang ◽  
Jung Soon Kwak ◽  
Seri Jang ◽  
Yuan Jia ◽  
Inshik Park
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Mixed Culture ◽  
Bacillus Sp

scholarly journals Screening and Characterisation of Keratin-Degrading Bacillus sp. from Feather Waste

2019 ◽  
pp. 1-12
Author(s):  
M. T. Dada ◽  
S. M. Wakil
Keyword(s):  
Bacillus Subtilis ◽  
Proteolytic Activity ◽  
Bacillus Licheniformis ◽  
Basal Medium ◽  
Bacillus Species ◽  
Bacillus Sp ◽  
Feather Degradation ◽  
Feather Waste ◽  
Degrading Bacteria ◽  
Significant Difference

Aim: This study focuses on the screening and characterisation of keratin-degrading Bacillus species from feather waste. Methods: Nine bacteria were isolated from feather waste obtained from a poultry layout at Egbeda local government secretariat, Ibadan, Nigeria. These bacteria were grown in basal medium with feather as primary source of carbon, nitrogen, sulfur and energy. Feather degrading bacteria were screened for both proteolytic activity and keratin degradation on skimmed milk agar and keratin azure medium respectively. They were also screened for their ability to degrade other keratin substrates such as hair and nail. Results: Three of the isolates with higher feather degradation levels also showed high proteolytic activity and release of azure dye. They were selected and identified phenotypically and genotypically using 16S rRNA sequencing as Bacillus licheniformis-K51, Bacillus subtilis-K50 and Bacillus sp.-K53. The bacteria were capable of degrading other keratin-containing substrates such as nail and hair. Bacillus subtilis-K50 and Bacillus licheniformis-K51 showed significant difference (P) in degradation among the three different keratin sources used yielding higher degradation with feather as keratin source with respective optical densities of 0.07 and 0.11 followed by hair and least in nails with optical densities of 0.05 and 0.07 respectively. Highest degradation of all the three keratin substrates was observed in Bacillus licheniformis-K51. Conclusion: The three isolated bacteria possess the ability to degrade keratin and utilize feather as keratin substrate. As a result, these can be considered as potential candidates for degradation and utilization of feather keratin.

scholarly journals Effect of plant growth promoting rhizobacteria (PGPR) and IBA treatments on rooting in cuttings of apple (Malus × domestica Borkh.) clonal rootstock Merton 793

2017 ◽  
Vol 9 (2) ◽  
pp. 1135-1138
Author(s):  
Pramod Verma ◽  
P. S. Chauhan ◽  
J. S. Chandel
Keyword(s):  
Plant Growth ◽  
Bacillus Licheniformis ◽  
Dry Weight ◽  
Plant Growth Promoting Rhizobacteria ◽  
Bacillus Sp ◽  
Main Shoot ◽  
Primary Roots ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Different Strains

The preliminary studies on the effect of different strains of plant growth promoting rhizobacteria (PGPR) alone and in combination with IBA at 1000 ppm on rooting in cuttings of apple clonal rootstock Merton 793 were carried out during 2012-13. The PGPR strains (RG (1)3 – Bacillus sp.), B6 – Bacillus licheniformis and R3 (3) – Sirretia sp. alone failed to induce rooting response in cuttings of apple clonal rootstock Merton 793. The results revealed that IBA 2500 ppm recorded the maximum rooting (65 %), number of primary roots (5.00), length (28.43 cm) and diameter (3.25 mm) of primary roots, fresh (3.67 g) and dry weight (2.59 g) of roots, length of main shoot (134.14cm), diameter of main shoot (8.18 mm), fresh (30.40 g) and dry weight (22.60 g) of shoots in cuttings of Merton 793. However, the PGPR strains RG (1)3 – Bacillus sp., B6 – Bacillus licheniformis and R3 (3) – Sirretia sp. in combination with IBA 1000 ppm showed improvement in rooting of cuttings to the extent of 10, 15 and 5 per cent rooting, respectively and growth of the rooted plants. IBA at 2500 ppm resulted better rooting and growth of rooted plants. Hence, this treatment is suggested for commercial propagation of apple clonal rootstock Merton 793 through cuttings.

scholarly journals Evaluación del crecimiento de cuatro especies del género Bacillus sp., primer paso para entender su efecto biocontrolador sobre Fusarium sp.

Nova ◽  
2016 ◽  
Vol 14 (26) ◽  
pp. 53 ◽  
Author(s):  
Estefania Castañeda Alvarez ◽  
Ligia Consuelo Sánchez
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Cereus ◽  
Bacillus Licheniformis ◽  
Bacillus Sp ◽  
Fusarium Sp

<p>Evaluar las condiciones de crecimiento de cuatro especies de <em>Bacillus </em>sp. nativas a escala de 10ml en Medio Mínimo de Sales (MMS) como primer paso para entender su acción biocontroladora contra <em>Fusarium </em>sp. <strong>Métodos. </strong>El procedimiento para evaluar el crecimiento de los aislamientos UCMC-TB1, UCMC-TB2, UCMC-TB3 y UCMC-TB4 se realizó utilizando espectrofotometría y recuento directo en placa y pruebas de antagonismo dual en placa para evaluar el efecto controlador contra <em>Fusarium </em>sp. <strong>Resultados. </strong>Se confirmó la identificación por pruebas bioquímicas de los cuatro aislamientos: <em>Bacillus licheniformis, Bacillus subtilis, Bacillus </em><em>pumilus y Bacillus cereus; </em>todas las cepas presentaron antagonismo <em>in vitro</em>. El <em>Bacillus subtilis </em>fue la especie que demostró mayor capacidad antagónica (79,73%PICR) y las características más destacadas de esta cepa fueron su velocidad de crecimiento. El género <em>Bacillus </em>es uno de los más reportados para usar en el control biológico de hongos como <em>Fusarium </em>sp. el cual ataca un gran número de cultivos de interés económico para el sector agrícola en Colombia.</p>

scholarly journals Características parcial del gen B-Galactosidasa de Bacillus sp. Msp7 aislado de las salinas de Pilluana, San Martín - Perú

2013 ◽  
Vol 16 (1) ◽  
pp. 18-23
Author(s):  
Amparo I. Zavaleta ◽  
Joseph Ávila ◽  
Elizabeth L. Chávez-Hidalgo ◽  
Victor Izaguirre
Keyword(s):  
Escherichia Coli ◽  
Bacillus Licheniformis ◽  
Bacillus Sp ◽  
Escherichia Coli Jm109 ◽  
San Martín

Las β-galactosidasas (EC.3.2.1.23) son glicosil hidrolasas que catalizan principalmente la hidrólisis de β-D-galactósidos, son producidas por diversos tipos de microorganismos y de interés en la síntesis de oligosacáridos. Una de las fuentes potenciales para la obtención de enzimas que cumplan estas características son los microorganismos aislados de ambientes extremos como las salinas. En este estudio, se clonó y caracterizó parcialmente el gen β-galactosidasa de Bacillus sp. MSP7, aislado de las salinas de Pilluana (San Martín), Perú, el cual presentó actividad enzimática de 65 U/mg de células secas, mayor que otras cuatro cepas de Bacillus sp. aisladas del mismo lugar. Con este fin, el gen en cuestión se amplificó mediante la reacción en cadena de la polimerasa usando cebadores específicos, obteniéndose un amplicón de aproximadamente 2000 pb; el producto se purificó y unió al vector pUC19, utilizando Escherichia coli JM109; los clones recombinantes fueron secuenciados parcialmente. Las secuencias nucleotídicas y aminoacídicas se analizaron mediante herramientas bioinformáticas, tales como Blast, Clustal y Mega. La secuencia nucleotídica de 957 pb correspondiente al gen β-galactosidasa de Bacillus sp MSP7, presentó 98% de similitud con el de Bacillus licheniformis ATCC 14580 (DSM 13) y a nivel aminoacídico, mostró 99% de similitud con las mismas cepas. La proteína pertenece a la familia GH 42.

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