scholarly journals Evaluación del crecimiento de cuatro especies del género Bacillus sp., primer paso para entender su efecto biocontrolador sobre Fusarium sp.

Nova ◽  
2016 ◽  
Vol 14 (26) ◽  
pp. 53 ◽  
Author(s):  
Estefania Castañeda Alvarez ◽  
Ligia Consuelo Sánchez
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Cereus ◽  
Bacillus Licheniformis ◽  
Bacillus Sp ◽  
Fusarium Sp

<p>Evaluar las condiciones de crecimiento de cuatro especies de <em>Bacillus </em>sp. nativas a escala de 10ml en Medio Mínimo de Sales (MMS) como primer paso para entender su acción biocontroladora contra <em>Fusarium </em>sp. <strong>Métodos. </strong>El procedimiento para evaluar el crecimiento de los aislamientos UCMC-TB1, UCMC-TB2, UCMC-TB3 y UCMC-TB4 se realizó utilizando espectrofotometría y recuento directo en placa y pruebas de antagonismo dual en placa para evaluar el efecto controlador contra <em>Fusarium </em>sp. <strong>Resultados. </strong>Se confirmó la identificación por pruebas bioquímicas de los cuatro aislamientos: <em>Bacillus licheniformis, Bacillus subtilis, Bacillus </em><em>pumilus y Bacillus cereus; </em>todas las cepas presentaron antagonismo <em>in vitro</em>. El <em>Bacillus subtilis </em>fue la especie que demostró mayor capacidad antagónica (79,73%PICR) y las características más destacadas de esta cepa fueron su velocidad de crecimiento. El género <em>Bacillus </em>es uno de los más reportados para usar en el control biológico de hongos como <em>Fusarium </em>sp. el cual ataca un gran número de cultivos de interés económico para el sector agrícola en Colombia.</p>

scholarly journals Biocontrole da mancha-aquosa do melão pelo tratamento de sementes com bactérias epifíticas e endofíticas

2006 ◽  
Vol 24 (3) ◽  
pp. 373-377 ◽  
Author(s):  
Aldenir de Oliveira ◽  
Marcos H M dos Santos ◽  
Elineide B da Silveira ◽  
Andréa M A Gomes ◽  
Rosa de LR Mariano
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Cereus ◽  
Bacillus Sp

Bactérias epifíticas e endofíticas (96 isolados) e fungos endofíticos (69 isolados) foram obtidos de plantas de meloeiro sadios e testados no controle da mancha-aquosa, em condições de casa de vegetação, pelo tratamento de sementes pré-inoculadas com Acidovorax avenae subsp. citrulli ou pelo tratamento de sementes sadias visando a proteção da planta a posterior inoculação com o patógeno. As sementes de melão foram microbiolizadas por imersão nas suspensões (A570= 0,7), semeadas e avaliadas quanto ao período de incubação (PI), incidência (INC), severidade da doença (SEV) e redução da severidade da doença (RSD). Apenas a microbiolização de sementes artificialmente infectadas, utilizando os endofíticos ENM5 (não identificado), ENM9 (Bacillus cereus), ENM13 (Bacillus sp.), ENM16 (Bacillus cereus), ENM32 (Bacillus subtilis) e ENM43 (Bacillus sp.), revelou potencial para o controle da mancha-aquosa. Esses isolados, após o teste de compatibilidade in vitro, foram reavaliados isoladamente e em misturas dois a dois quanto ao PI, INC, SEV e RSD, além do índice de doença (IDO) e área abaixo da curva de progresso da doença (AACPD). Todos os tratamentos diferiram significativamente (P= 0,05) da testemunha, com RSD de até 93,6%, destacando-se os isolados ENM13 e ENM9 com PI de 7,5 e 7,25 dias, SEV de 0,22 e 0,22, IDO de 2,59 e 2,59, e AACPD de 0,22 e 0,39, respectivamente. Ensaios foram realizados in vitro para a determinação dos possíveis mecanismos de ação envolvidos no controle biológico. Os isolados ENM13 e ENM9 solubilizaram fosfato, ENM5 apresentou antibiose contra A. avenae subsp. citrulli, ENM43 produziu HCN enquanto ENM16 e ENM32 não apresentaram nenhum dos mecanismos testados.

scholarly journals BIOCONTROLE DE Macrophomina phaseolina E PROMOÇÃO DO CRESCIMENTO NA CULTURA DO GIRASSOL COM O USO DE Trichoderma sp. E RIZOBACTÉRIAS

2021 ◽  
Vol 5 (1) ◽  
Author(s):  
Nelrilene Pereira da Silva ◽  
Marta Cristina Corsi de Filippi ◽  
Fábio José Gonçalves ◽  
Alan Carlos Alves de Souza
Keyword(s):  
Bacillus Subtilis ◽  
Macrophomina Phaseolina ◽  
Bacillus Sp ◽  
Trichoderma Sp

Fungos fitopatogênicos habitantes do solo como Macrophomina phaseolina são de difícil controle, principalmente por formarem estruturas de resistência. Por se tratar de uma espécie polífaga, faz-se necessário realizar estudos para se obter diferentes formas efetivas de controle. Diante disso, o objetivo deste trabalho foi testar a eficiência de microrganismos benéficos no controle biológico de M. phaseolina, e na promoção de crescimento à cultura do girassol. A presente pesquisa foi realizada em duas etapas, uma com ensaio in vitro e outra com ensaio in vivo. O ensaio in vitro foi conduzido no laboratório de análises microbiológicas AgroLab, onde realizou-se teste de pareamento de culturas com seis tratamentos e três repetições, sendo, (T1 - Trichoderma sp. + patógeno; T2 - Bacillus sp. + patógeno; T3 – B. pyrrocinia + patógeno; T4 – P. fluorescens + patógeno; T5 – B. subtilis + patógeno e T6 - somente o patógeno), avaliou-se a ocorrência de antibiose e selecionou-se os isolados mais promissores para o ensaio in vivo. O ensaio in vivo foi realizado na Unidade Experimental do laboratório AgroLab, em recipientes de 400 mL, em cultivo protegido, sendo conduzido em Delineamento Inteiramente Casualizado com cinco tratamentos (T1 – controle; T2 - Trichodermil®; T3 - Trichoderma sp.; T4 - B. pyrrocinia e T5 - B. subtilis) em oito repetições. Foram avaliados a capacidade dos bioagentes em suprimir a severidade da doença e, sua eficiência como promotores de crescimento para a cultura do girassol. Os parâmetros avaliados nas análises de crescimento foram: comprimento de raiz e parte aérea e, biomassa da raiz e parte aérea. Os resultados mostraram diferença significativa entre si pelo teste de F (p < 0,05). Nos testes in vitro, os isolados de Trichoderma sp., Bacillus subtilis e B. pyrrocinia reportaram melhor atividade antagônica com 10,93%, 10,26% e 3,71% de inibição do crescimento micelial do patógeno, respectivamente. Na promoção de crescimento todos os tratamentos promoveram maior comprimento da raiz e aumento da biomassa da parte aérea. Não houve diferença significativa para comprimento da parte aérea em relação a testemunha, e os isolados de B. subtilis, B. pyrrocinia e Trichoderma sp. apresentaram maior biomassa da raiz. Na severidade da doença os tratamentos com B. subtilis, B. pyrrocinia, Trichoderma sp. e Trichodermil®, apresentaram 90,5%, 81,0%, 81,0% e 62,5% de supressão do patógeno, respectivamente. Concluiu-se que estes microrganismos apresentam potencial como promotores de crescimento e biocontroladores da doença, podendo tornar-se técnica viável a ser inserida ao Manejo Integrado de Doenças.

scholarly journals Screening and Characterisation of Keratin-Degrading Bacillus sp. from Feather Waste

2019 ◽  
pp. 1-12
Author(s):  
M. T. Dada ◽  
S. M. Wakil
Keyword(s):  
Bacillus Subtilis ◽  
Proteolytic Activity ◽  
Bacillus Licheniformis ◽  
Basal Medium ◽  
Bacillus Species ◽  
Bacillus Sp ◽  
Feather Degradation ◽  
Feather Waste ◽  
Degrading Bacteria ◽  
Significant Difference

Aim: This study focuses on the screening and characterisation of keratin-degrading Bacillus species from feather waste. Methods: Nine bacteria were isolated from feather waste obtained from a poultry layout at Egbeda local government secretariat, Ibadan, Nigeria. These bacteria were grown in basal medium with feather as primary source of carbon, nitrogen, sulfur and energy. Feather degrading bacteria were screened for both proteolytic activity and keratin degradation on skimmed milk agar and keratin azure medium respectively. They were also screened for their ability to degrade other keratin substrates such as hair and nail. Results: Three of the isolates with higher feather degradation levels also showed high proteolytic activity and release of azure dye. They were selected and identified phenotypically and genotypically using 16S rRNA sequencing as Bacillus licheniformis-K51, Bacillus subtilis-K50 and Bacillus sp.-K53. The bacteria were capable of degrading other keratin-containing substrates such as nail and hair. Bacillus subtilis-K50 and Bacillus licheniformis-K51 showed significant difference (P) in degradation among the three different keratin sources used yielding higher degradation with feather as keratin source with respective optical densities of 0.07 and 0.11 followed by hair and least in nails with optical densities of 0.05 and 0.07 respectively. Highest degradation of all the three keratin substrates was observed in Bacillus licheniformis-K51. Conclusion: The three isolated bacteria possess the ability to degrade keratin and utilize feather as keratin substrate. As a result, these can be considered as potential candidates for degradation and utilization of feather keratin.

scholarly journals SKRINING DAN IDENTIFIKASI BAKTERI ANTI QUORUM SENSING ASAL TAMBAK UDANG VANAME PENGHAMBAT VIRULENSI Vibrio parahaemolyticus

2021 ◽  
Vol 16 (1) ◽  
pp. 61
Author(s):  
Emei Widiyastuti ◽  
Iman Rusmana ◽  
Munti Yuhana
Keyword(s):  
Quorum Sensing ◽  
16S Rrna ◽  
Bacillus Cereus ◽  
Vibrio Parahaemolyticus ◽  
Bacillus Amyloliquefaciens ◽  
Chromobacterium Violaceum ◽  
Pathogenicity Test ◽  
Bacillus Sp ◽  
Communication Signals

Vibriosis dapat dicegah dan dikendalikan dengan memanfaatkan mekanisme anti quorum sensing (AQS). Salah satu strategi anti quorum sensing dalam menghambat ekspresi faktor virulen dari Vibrio parahaemolyticus yaitu dengan mendegradasi sinyal komunikasi sel bakteri menggunakan AHL laktonase. Penelitian ini bertujuan untuk menyeleksi dan mengindentifikasi bakteri penghasil AHL laktonase yang berpotensi mampu menghambat virulensi bakteri patogen V. parahaemolyticus. Isolasi bakteri dilakukan dari sampel saluran pencernaan udang vaname, air, dan sedimen tambak. Sebanyak 18 dari 111 isolat yang diisolasi menunjukkan adanya aktivitas AQS terhadap bioindikator Chromobacterium violaceum. Hasil uji patogenitas secara in vitro pada agar darah didapatkan tiga isolat yang tidak menunjukkan aktivitas hemolisis yaitu B5, K4, dan S12. Hasil konfirmasi dan analisis gen aiiA menggunakan Blast-X menunjukkan bahwa isolat B5 dan S12 memiliki kesamaan dengan AHL laktonase pada Bacillus cereus, sedangkan K4 memiliki similaritas dengan AHL laktonase pada multispesies Bacillus sp. Hasil pensejajaran sekuen gen 16S rRNA ketiga isolat tersebut dengan data pada GenBank, teridentifikasi sebagai Bacillus siamensis (B5), Bacillus cereus (K4), dan Bacillus amyloliquefaciens (S12). Berdasarkan hasil uji antagonis dan uji kultur bersama disimpulkan bahwa isolat K4 bekerja dengan mekanisme AQS sedangkan isolat B5 dan S12 diduga berjalan dua mekanisme secara bersama yaitu antibiosis dan anti quorum sensing. Hasil penelitian ini menunjukkan bahwa ketiga isolat tersebut memiliki potensi sebagai kandidat agen biokontrol pada akuakultur sehingga perlu dilakukan uji lanjutan.Vibriosis can be prevented and controlled by utilizing the anti-quorum sensing (AQS) mechanism. One of the anti-quorum sensing mechanisms to inhibit the expression of virulent factors of Vibrio parahaemolyticus is by degrading the quorum sensing communication signals using AHL lactonase. The study aimed to select and identify AHL lactonase-producing bacteria that have the potentials to inhibit the virulence of V. parahaemolyticus. Several batches of bacteria were isolated from the digestive tract of vannamei shrimp, water, and sediment of shrimp ponds. There were 18 out of 111 isolates that showed AQS activity against Chromobacterium violaceum used as a bioindicator. In vitro pathogenicity test on blood agar showed that B5, K4, and S12 isolates showed gamma hemolysis activity. The results of confirmation and analysis of aiiA genes using Blast-X showed that B5 and S12 isolates have AHL lactonase similarities with Bacillus cereus, whereas K4 has similarities with multispecies Bacillus sp. Alignment results of the 16S rRNA gene sequences with GenBank data showed that B5, K4, and S12 isolates were identified as Bacillus siamensis, Bacillus cereus, and Bacillus amyloliquefaciens, respectively. The follow up antagonistic and coculture tests revealed that K4 uses the AQS mechanism, while B5 and S12 likely use antibiotic mechanism and anti quorum sensing to inhibit the virulent expression of V. parahaemolyticus. This study concludes that the three isolates have the potential to be used as biocontrol agents in brackishwater aquaculture. Further research is needed to determine the pathogenicity of AQS bacteria to vannamei shrimp and the effective concentration of AQS bacteria to inhibit the virulence of V. parahaemolyticus to vannamei shrimp by in vivo treatment.  

scholarly journals Synthesis, Antibacterial, Thrombolytic and Cytotoxic Evaluation of Substituted and Un-substituted Selenium-n-heterocyclic Carbene Adducts

2022 ◽  
Author(s):  
Amna Kamal ◽  
Muhammad Adnan Iqbal ◽  
Haq Nawaz Bhatti ◽  
Abdul Ghaffar
Keyword(s):  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Bacillus Cereus ◽  
Room Temperature ◽  
Inhibition Zone ◽  
Spectroscopic Techniques ◽  
Mouse Blood ◽  
Ft Ir ◽  
Better Than

Abstract N-heterocyclic carbene salts bearing alkyl substituents (1-8) and their selenium N-heterocyclic carbene adducts (9-12) were synthesized and characterized by elemental analysis, FT-IR, NMR (1HNMR, 13CNMR) spectroscopic techniques. All the adducts were found to be stable in air and moisture at room temperature. Compounds (5-12) were evaluated against Bacillus subtilis Macrococcus brunensis and Bacillus cereus in vitro. The biological assay revealed that antibacterial activity of Selenium-N-heterocyclic carbene adducts are comparatively better than the salts. MIC and inhibition zone values showed that Bacillus subtilis is more active to selenium adducts (9-12) than Macrococcus brunensis and Bacillus cereus whereas opposite in the salts (5-8). In vitro studies of hemolysis and thrombolysis demonstrated that the synthesized compounds are innocuous for pre-clinical trials to mouse blood.

Dependence of the Biological Activity of the Permafrost Bacteria Bacillus Sp. on Temperature

10.12737/7288 ◽  
2014 ◽  
Vol 21 (4) ◽  
pp. 142-148
Author(s):  
Субботин ◽  
A. Subbotin ◽  
Бажин ◽  
A. Bazhin ◽  
Калёнова ◽  
...  
Keyword(s):  
Biological Activity ◽  
Enzymatic Activity ◽  
Bacillus Cereus ◽  
Low Temperatures ◽  
Incubation Temperature ◽  
Probiotic Bacteria ◽  
Laboratory Animals ◽  
Bacillus Sp

Fossil bacteria in permafrost evolutionarily adapted to low temperatures (-5ºC), modern probiotics bacteria are adapted to living in warm-blooded animals (37ºC). It was found that at -5ºC, the enzymatic activity of fossil bacteria Bacillus sp. MG8 is a minimal strain. At lowering the incubation temperature to -16ºC, the enzymatic activity of bacte-ria MG8 increases in 3 times, at the temperature 42ºC - in a 1.5times relative IP5832 strain probiotic bacteria Bacillus cereus. Fossil strain Bacillus sp. MG8 and probiotic bacterial strain B.cereus IP5832 at incubation temperature 37ºC practically don’t differ from each other in the enzymatic activity in vitro and toxicity in laboratory animals in vivo. Incubation fossil bacteria Bacillus sp. at -5ºC allows to reduce their toxicity in warm-blooded animals in 5 times in comparison with Bacillus cereus JP5832, and to increase immunostimulating effect in the doses from 0,005•106 to 50•106 microbial cells per mouse. The obtained data show that fossil saprophytic bacteria strain MG8 Bacillus sp. from permafrost are less toxic to modern mammals than even bacilli-probiotics for medical purposes.

Detection and quantification of spoilage and pathogenic Bacillus cereus, Bacillus subtilis and Bacillus licheniformis by real-time PCR

2011 ◽  
Vol 28 (3) ◽  
pp. 605-610 ◽  
Author(s):  
I.C. Fernández-No ◽  
M. Guarddon ◽  
K. Böhme ◽  
A. Cepeda ◽  
P. Calo-Mata ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Cereus ◽  
Real Time ◽  
Bacillus Licheniformis ◽  
Real Time Pcr ◽  
Detection And Quantification

scholarly journals RECONSTITUTION OF DIFFERENT SIGNAL PEPTIDES FOR ENHANCED THERMOSTABLE ALPHA AMYLASE SECRETION IN Bacillus subtilis

2018 ◽  
Vol 56 (1) ◽  
pp. 7 ◽  
Author(s):  
Nguyen Thị Da ◽  
Tran Dinh Man ◽  
Nguyen Kim Thoa
Keyword(s):  
Molecular Weight ◽  
Bacillus Subtilis ◽  
Bacillus Cereus ◽  
Bacillus Licheniformis ◽  
Signal Peptide ◽  
Amylase Activity ◽  
Alpha Amylase ◽  
Amylase Secretion ◽  
Signal Peptides ◽  
Extracellular Amylase

Three signal peptides of alpha amylase genes of three isolated strains that were Bacillus licheniformis DA23, Bacillus subtilis D5-2, Bacillus cereus CN1-5 were successfully sequenced. Three predicted “Sec – type” signal peptides have a length varying from 27 (CN1-5) to 33 residues (D5-2).  The secretion of alpha amylase of the recombination B. subtilis 168MPgrac strain (pHV33–PgracAmy3BT2) with 71.4U/ml was larger than that of 168MPamy with 53.2U/ml. Base on analyzed rerults of PAGE and zymogram about molecular weight, alpha amylases in both strains were the same size, nearly 58kDa. The extracellular amylase activity of signal peptide SsubtilisD5.2 in 168M was highest with 76.4±4.3 U/ml in four signal peptide targets.  

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