Chapter 7: Seed health testing

2022 ◽  
Vol 2022 (1) ◽  
pp. i-7-6
Keyword(s):  
2021 ◽  
Vol 161 ◽  
pp. 113186
Author(s):  
Clíssia Barboza da Silva ◽  
Vitor de Jesus Martins Bianchini ◽  
André Dantas de Medeiros ◽  
Maria Heloisa Duarte de Moraes ◽  
Agide Gimenez Marassi ◽  
...  

2019 ◽  
Vol 20 (11) ◽  
pp. 2715 ◽  
Author(s):  
Md. Rafiqul Islam ◽  
Mohammad Rashed Hossain ◽  
Hoy-Taek Kim ◽  
Denison Michael Immanuel Jesse ◽  
Md. Abuyusuf ◽  
...  

Acidovorax citrulli (A. citrulli) strains cause bacterial fruit blotch (BFB) in cucurbit crops and affect melon significantly. Numerous strains of the bacterium have been isolated from melon hosts globally. Strains that are aggressively virulent towards melon and diagnostic markers for detecting such strains are yet to be identified. Using a cross-inoculation assay, we demonstrated that two Korean strains of A. citrulli, NIHHS15-280 and KACC18782, are highly virulent towards melon but avirulent/mildly virulent to the other cucurbit crops. The whole genomes of three A. citrulli strains isolated from melon and three from watermelon were aligned, allowing the design of three primer sets (AcM13, AcM380, and AcM797) that are specific to melon host strains, from three pathogenesis-related genes. These primers successfully detected the target strain NIHHS15-280 in polymerase chain reaction (PCR) assays from a very low concentration of bacterial gDNA. They were also effective in detecting the target strains from artificially infected leaf, fruit, and seed washing suspensions, without requiring the extraction of bacterial DNA. This is the first report of PCR-based markers that offer reliable, sensitive, and rapid detection of strains of A. citrulli causing BFB in melon. These markers may also be useful in early disease detection in the field samples, in seed health tests, and for international quarantine purposes.


Mycologia ◽  
1994 ◽  
Vol 86 (5) ◽  
pp. 713
Author(s):  
Lori M. Carris ◽  
S. B. Mathur ◽  
Barry M. Cunfer
Keyword(s):  

1967 ◽  
Vol 73 (1) ◽  
pp. 1-14 ◽  
Author(s):  
T. Limonard

2013 ◽  
Vol 14 (1) ◽  
pp. 41 ◽  
Author(s):  
Y. He ◽  
G. P. Munkvold

Xanthomonas axonopodis pv. phaseoli (Smith) Vauterin (Xap) and Xanthomonas fuscans subsp. fuscans Schaad et al. (Xff) cause indistinguishable symptoms known as common bacterial blight of bean (Phaseolus vulgaris L.). These results confirm a higher disease incidence and seed transmission frequency of Xff compared to Xap and reinforce the need for seed health tests that can differentiate the two species. Accepted for publication 21 August 2013. Published 23 September 2013.


2012 ◽  
Vol 34 (4) ◽  
pp. 541-548 ◽  
Author(s):  
Ísis Barreto Dantas ◽  
João Almir de Oliveira ◽  
Heloisa Oliveira dos Santos ◽  
Édila Vilela Resende Von Pinho ◽  
Sttela Dellyzete Veiga Franco da Rosa

Seed quality may be affected by several factors, including permeability, color, and lignin content in the seed coat. This study aimed at evaluating influence of lignin content in the tegument of seed samples of six different soybean cultivars, in which half of each sample was inoculated with the fungus Aspergillus flavus, on the physical and physiological quality, and on the seed health, during 180 days storage period, under cold chamber with controlled conditions of temperature and RH. For that, at each interval of 60 days, samples were removed, and the physiological quality of these seeds was assessed by means of moisture and lignin contents; and by tests of seed health, germination, and electrical conductivity. The moisture content of seeds remained constant during all storage period. In the seed health test, it was found that inoculation was efficient, once the minimum incidence of the fungus in the inoculated seeds was 85%. In the germination test, there was a trend of reduction on percentage germination with the increase in storage period. However, there was an increase on electrical conductivity of seeds assessed. It was concluded that there is no interference of the lignin content in the seed coat on the resistance to infection by the fungus Aspergillus flavus, even after seed storage for a period of 180 days.


Author(s):  
Atul Kumar ◽  
Ashok Gaur ◽  
Malkhan Singh Gurjar ◽  
Pooja Kumari ◽  
Raj Kiran

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