Determination and equilibration of seed moisture content (SMC) and seed storage of black wattle (Acacia mearnsii) seed for gamma irradiation

Soybean seed viability declines during seed storage. Soybean seed deteriorates rapidly, affected by its high protein content and often high humidity in the tropical environment. This research was aimed to develop dynamic model of soybean seed viability in an open storage. The study was conducted in three stages, namely: 1) desk study, 2) seed storage experiment, 3) development of seed storage model, simulation and verification of the model. The second stage of the experiment consisted of soybean seed storing and germination testing using completely randomized design. Treatments were three initial moisture content (7-8%, 9-10% and 11-12%) and four varieties of soybean (Anjasmoro, Wilis, Detam-1 and Detam-2). The results showed that the seed behaviour during storage period were affected by initial seed moisture content, initial viability, varieties and environmental condition. Therefore, seed moisture content, initial viability and varieties may be used as input model. Moisture content, integreting seed respiration, electric conductivity and seed storability vigor (VDSDB) were as model output. Simulation of Seed Storability Vigor Prediction Model with Model Construction Layer-Stella (MCLS) using relative humidity (RH), temperature, seed permeability, initial moisture content and initial viability as input model could logically predict the seed moisture content and seed storability vigor (VDSDB).

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Evaluation of Temperature and Moisture Content during Storage on the Germination of Flowering Annual Seed

HortScience ◽

10.21273/hortsci.30.5.1003 ◽

1995 ◽

Vol 30 (5) ◽

pp. 1003-1006 ◽

Cited By ~ 4

Author(s):

William J. Carpenter ◽

Eric R. Ostmark ◽

John A. Cornell

Keyword(s):

Relative Humidity ◽

Moisture Content ◽

Seed Storage ◽

Seed Moisture Content ◽

Moisture Contents ◽

Seed Moisture ◽

Storage Temperatures

Various combinations of temperature and moisture contents were used in evaluating the seed storage of nine genera of annual flowers. Relative humidity (RH) levels of 11%, 32%, 52%, and 75% provided wide ranges in seed moisture during storage at 5, 15, and 25C. At each temperature, total germination percentages (G) generally declined as seed moisture content increased during storage. The seed moisture range giving the highest G after 12 months of storage was determined for each temperature and plant genus. For all genera, seed moisture contents during storage increased as storage temperatures increased at constant RH levels. Moisture contents at 25C storage were 37%, 34%, 29%, and 20% higher than at 5C when RH levels were at 11%, 32%, 52%, and 75%, respectively.

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CHROMOSOME DAMAGE INDUCED BY ARTIFICIAL SEED AGING IN BARLEY. I. GERMINABILITY AND FREQUENCY OF ABERRANT ANAPHASES AT FIRST MITOSIS

Canadian Journal of Genetics and Cytology ◽

10.1139/g81-029 ◽

1981 ◽

Vol 23 (2) ◽

pp. 267-280 ◽

Cited By ~ 23

Author(s):

Minoru Murata ◽

Eric E. Roos ◽

Takumi Tsuchiya

Keyword(s):

Moisture Content ◽

Seed Storage ◽

Germination Percentage ◽

Mitotic Division ◽

Root Tips ◽

Hordeum Vulgare L ◽

Seed Moisture Content ◽

Seed Aging ◽

Seed Moisture ◽

Higher Temperature

In order to study the genetic changes which occur during seed storage, barley (Hordeum vulgare L. 'Himalaya') seeds were subjected to artificial aging using six combinations of temperature (21 °C, 32 °C, and 38 °C) and seed moisture content (12% and 18%). With increasing time in storage, germination of the seeds was delayed and reduced. Abnormal seedlings without roots also occurred with increased storage. Higher temperature and seed moisture content induced rapid loss of germinability. At the first mitotic division in the root tips, the frequency of aberrant anaphases and of roots with aberrations increased with increased storage time. The frequencies of aberrant anaphases and of roots with aberrations were also increased by higher temperature and seed moisture content. Frequencies of aberrant anaphases and of roots with aberrations were negatively correlated with germination percentage. This indicated that the frequency of chromosomal aberrations induced by seed aging might be estimated from the germination percentages.

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Conservation of Garcinia imberti Bourd. through seeds

Plant Science Today ◽

10.14719/pst.2019.6.2.518 ◽

2019 ◽

Vol 6 (2) ◽

pp. 243-251

Author(s):

M Anto ◽

M Angala ◽

P S Jothish ◽

C Anilkumar

Keyword(s):

Seed Germination ◽

Relative Humidity ◽

Moisture Content ◽

Gibberellic Acid ◽

Biosphere Reserve ◽

Seed Storage ◽

Seed Moisture Content ◽

Evergreen Forests ◽

Seed Moisture ◽

Subsequent Exposure

Garcinia imberti seeds were collected during 2015-2017 from Shangili, Cheenikkala and Bonaccord evergreen forests of Agasthyamala Biosphere Reserve, the only abode of this endangered endemic species. Germinability of seeds were analysed through decoating, Gibberellic acid (GA3) and light inductive pre-treatments on fresh (62.8 % moisture content; MC) and desiccated (fast; 23.3% MC and slow; 30.5 % MC) seeds. The seed germination with impermeable coat (0.7-1.2 mm) was restricted which on decoating got enhanced. Application of GA3 along with exposure to light breaked dormancy within 4-6 days compared to non-treated seeds that took 238-254 days to germinate. Stored seeds behaviour revealed that seed moisture content and rate of germination were negatively correlated. Seed storage was found to be more efficient only up to 80 days at controlled seed banking conditions (20 ± 20C, 20 % relative humidity; RH). Both fast and slow desiccated seeds stored for 60 days in seed bank conditions exhibited 50.4 and 43.4 % of germination compared 39.4% germination of non-desiccated seeds. Hence fast desiccated and decoated G. imberti seeds pre-treated with GA3 on subsequent exposure to light alleviated dormancy. For seed banking, fast desiccated seeds with MC in between 40-20% are found to be promising.

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Drying and physiological quality of palheteira seeds (Clitoria fairchildiana R.A. Howard)

Research Society and Development ◽

10.33448/rsd-v9i9.8157 ◽

2020 ◽

Vol 9 (9) ◽

pp. e858998157

Author(s):

Érica Coutinho David ◽

Bressa Karolina Dias Cardoso ◽

Josiene Amanda dos Santos Viana ◽

Eniel David Cruz

Keyword(s):

Moisture Content ◽

Seed Quality ◽

Seed Storage ◽

Germination Percentage ◽

Hot Water ◽

Degraded Land ◽

Seed Moisture Content ◽

Seed Moisture ◽

Physiological Quality

Knowledge about seed storage behavior is important to preserve plant species. Clitoria fairchildiana R.A.Howard is an endemic species with medicinal properties, it is used in the recovery of degraded land. The objective of this study is to evaluate the effect of drying on the physiological quality of C. fairchildiana seeds. Seeds were oven dried along with silica gel for 18 days and in intervals of 2 or 3 days, samples were removed to assess the seed moisture content. This was done in an oven at 105±3 oC for 24h, using four replicates with 10 seeds each. After undergoing the above-mentioned treatment, a sample of seeds was stored in the freezer (-18 ºC) for 3 months. Sowing was carried out in plastic trays in a sand and sawdust mixture (1:1), previously sterilized in hot water (100 oC) for two hours. Seeds were left to germinate in a laboratory with no temperature and relative humidity control. Germination tests were performed over 14 days with daily counts of the number of emerged seedlings. The percentage of seed germination, abnormal seedlings and dead seeds were obtained 14 days after sowing. The experimental design was completely randomized with four replications of 25 seeds. The reduction of seed moisture content from 18.5% to 5.6% affected physiological seed quality causing a reduction in the germination percentage and an increase in dead seeds and abnormal seedling percentage. C. fairchildiana seeds are classified as intermediate and they can be desiccated up to 8.6% with no reduction in physiology quality.

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Engineered Jute Bags for Storing Food Grains within Harrington’s Safe Zone of Seed Moisture Content

10.26434/chemrxiv.13664576 ◽

2021 ◽

Author(s):

Kennedy Odokonyero ◽

Himanshu Mishra ◽

Adair Gallo Junior

Keyword(s):

Moisture Content ◽

Low Cost ◽

Seed Storage ◽

Water Repellent ◽

Seed Moisture Content ◽

Post Harvest ◽

Food Grains ◽

Seed Moisture ◽

Jute Fabrics ◽

Jute Bags

Harrington’s thumb rule states that for every 1% increase in seed moisture content (SMC), seed lifetime decreases by 50%. Thus, to avoid post-harvest grain losses, stored seeds must be insulated from water. Although the jute bags typically used to store grains afford an ecofriendly, durable, and low-cost storage solution, their hydrophilic nature makes them vulnerable to wetting from airborne humidity and precipitation events. To address this issue, we treated jute fabrics with alkali and wax to render them water-repellent. A 2-month seed storage experiment revealed that wax-coated jute bags (WCJBs) outperformed control jute bags (CJBs) at safeguarding wheat (Triticum aestivum) grains exposed to 55%–98% relative humidity. Specifically, grains stored in WCJBs exhibited 15%–50% less SMC than grains stored in CJBs, which led to a 21%–66% enhancement in the germination efficacy of WCJB-stored seeds. This engineering solution could enhance the potential of jute bags to reduce post-harvest losses.

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Three-dimensional Models Represent Seed Moisture Content as a Function of Relative Humidity and Temperature

HortScience ◽

10.21273/hortsci.33.7.1207 ◽

1998 ◽

Vol 33 (7) ◽

pp. 1207-1209 ◽

Cited By ~ 5

Author(s):

Jian Fang ◽

Frank Moore ◽

Eric Roos ◽

Christina Walters

Keyword(s):

Relative Humidity ◽

Moisture Content ◽

Citrullus Lanatus ◽

Three Dimensional ◽

Selection Criterion ◽

Seed Storage ◽

Response Surfaces ◽

Seed Moisture Content ◽

Seed Moisture ◽

Three Dimensional Models

Seed moisture content (MC) has been considered the most important factor controlling physiological reactions in seeds, and MC changes with relative humidity (RH) and temperature (T). This relationship is revealed by studying the interaction of RH and T at equilibrium. Cucumber (Cucumis sativus L.), lettuce (Lactuca sativa L.), maize (Zea mays L.), onion (Allium cepa L.), pea (Pisum sativum L.), and watermelon (Citrullus lanatus M. & N.) seeds were equilibrated over sulfuric acid (1% RH) and various saturated salt solutions (5.5% to 93% RH) at temperatures from 5 to 50 °C. Best-fit subset models were selected from the complete third-order model MC = β0 + β1*RH + β2*T + β3*RH2 + β4*T2 + β5*RH*T + β6*RH3 + β7*T3 + β8*RH*T2 + β9*RH2*T, using Mallows' minimum Cp as the selection criterion. All six best subset models (R2, 0.98 to 0.99) had the same functional form, MC = β0 + β1*RH + β2*T + β3*RH2 + β5*RH*T + β6*RH3 + β9*RH2*T. Coefficients had essentially the same respective values among all species except onion and pea, for which some coefficients were statistically different from those of the other species (P ≤ 0.05). All models indicated that seed MC increased as RH increased and decreased as T increased; but RH had the greater influence. The inverse relationship between seed MC and T, although slight, was evident in the response surfaces. The interaction effect of RH and T on MC was significant at P ≤0.001. These results suggest that orthodox seed species respond similarly to T and RH. This in turn suggests that a common model could be developed and used for optimizing seed storage environments.

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The Relationship Among Storage Condition and Seed Moisture Content Revisited

HortScience ◽

10.21273/hortsci.33.3.490c ◽

1998 ◽

Vol 33 (3) ◽

pp. 490c-490

Author(s):

Jian Fang ◽

Frank Moore ◽

Eric E. Roos ◽

Christina Walters

Keyword(s):

Moisture Content ◽

Storage Temperature ◽

Selection Criterion ◽

Seed Storage ◽

Storage Condition ◽

Third Order ◽

Seed Moisture Content ◽

Seed Moisture ◽

Orthodox Seeds ◽

The Relationship

Seed moisture content (MC) changes depending upon relative humidity (RH) and temperature (T). This relationship was revealed by studying the interaction of RH and T at equilibrium. Maize, cucumber, onion, lettuce, watermelon, and pea seeds were equilibrated over various saturated salt solutions (1% to 93% RH) at temperatures from 5 to 50 °C. Five-hundred-eleven subset models were selected from a complete third-order model MC = 0 + 1*RH + 2*T + 3*RH2 + 4*T2 + 5*RH*T + 6*RH3 + 7*T3 + 8*RH*T2 + 9*RH2*T using Mallows' minimum Cp as the selection criterion. All six best subset models were found to have the same functional form, MC0 + 1 = *RH + 2*T + *RH2 + 5*RH*T + 6*RH + 9*RH2*T. Coefficients had essentially the same respective values among species, except for the onion and pea models (P ≤ 0.05). All models indicated that seed MC increased as RH increased and decreased as temperature increased. A previous paper (Vertucci and Roos, 1990) indicated that optimum RH for orthodox seed storage is between 19% and 27%. Using these models, the range for the optimum moisture content for storage of most orthodox seeds can be quickly calculated for any given storage temperature.

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Effect of Desiccation and Storage Environment on Longevity of Ehretia Cymosa Thonn. Seeds.

10.21203/rs.3.rs-43618/v1 ◽

2020 ◽

Author(s):

Peter Murithi Angaine ◽

Stephen Muriithi Ndungú ◽

Alice Adongo Onyango ◽

Jesse Omondi Owino

Keyword(s):

Moisture Content ◽

Storage Temperature ◽

Seed Viability ◽

Seed Storage ◽

Storage Duration ◽

Seed Moisture Content ◽

Seed Moisture ◽

Storage Behavior ◽

Storage Studies ◽

And Storage

Abstract Background: Globally, forestry faces challenges in the availability of seeds due to limited knowledge on seed handling of various species. Forestry seeds are constantly being reviewed and classified as either recalcitrant, intermediate, or orthodox based on their storage behavior. It is essential to understand the tree seed storage behavior to maintain seed viability and thus minimize seed losses. There is scanty literature combining factors of seed moisture content (6%, 9%, 12%, 15%, and 20%), seed storage temperature (20oC, 5oC and -20oC), seed storage duration (1, 4, 9 and 12 months), and germination in different sites with varying environmental variables. Ehretia cymosa is important in the Afromontane forestry landscape as a medicinal, rehabilitation, and conservation species. This study conducted desiccation and storage studies and their influence on the viability of E. cymosa seeds. The study sought to determine the optimum conditions for the storage of Ehretia cymosa that maintains viability. Results: This study observed that E. cymosa dried to seed moisture content of 6%, stored for 12 months at 20oC and sown in the laboratory had the highest germination performance (27.6 ± 3.18%) (p<0.05). Conclusion: This confirms that E.cymosa seeds exhibit orthodox storage behavior. The authors recommend longer storage studies (>12months) to determine the actual longevity of the seeds of this species. The significance of these results would be useful for foresters and farmers that would need to use this species for various purposes.

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Engineered Jute Bags for Storing Food Grains within Harrington’s Safe Zone of Seed Moisture Content

10.26434/chemrxiv.13664576.v1 ◽

2021 ◽

Author(s):

Kennedy Odokonyero ◽

Himanshu Mishra ◽

Adair Gallo Junior

Keyword(s):

Moisture Content ◽

Low Cost ◽

Seed Storage ◽

Water Repellent ◽

Seed Moisture Content ◽

Post Harvest ◽

Food Grains ◽

Seed Moisture ◽

Jute Fabrics ◽

Jute Bags

Harrington’s thumb rule states that for every 1% increase in seed moisture content (SMC), seed lifetime decreases by 50%. Thus, to avoid post-harvest grain losses, stored seeds must be insulated from water. Although the jute bags typically used to store grains afford an ecofriendly, durable, and low-cost storage solution, their hydrophilic nature makes them vulnerable to wetting from airborne humidity and precipitation events. To address this issue, we treated jute fabrics with alkali and wax to render them water-repellent. A 2-month seed storage experiment revealed that wax-coated jute bags (WCJBs) outperformed control jute bags (CJBs) at safeguarding wheat (Triticum aestivum) grains exposed to 55%–98% relative humidity. Specifically, grains stored in WCJBs exhibited 15%–50% less SMC than grains stored in CJBs, which led to a 21%–66% enhancement in the germination efficacy of WCJB-stored seeds. This engineering solution could enhance the potential of jute bags to reduce post-harvest losses.

Download Full-text