scholarly journals Evaluation of Temperature and Moisture Content during Storage on the Germination of Flowering Annual Seed

HortScience ◽  
1995 ◽  
Vol 30 (5) ◽  
pp. 1003-1006 ◽  
Author(s):  
William J. Carpenter ◽  
Eric R. Ostmark ◽  
John A. Cornell
Keyword(s):  
Relative Humidity ◽  
Moisture Content ◽  
Seed Storage ◽  
Seed Moisture Content ◽  
Moisture Contents ◽  
Seed Moisture ◽  
Storage Temperatures

Various combinations of temperature and moisture contents were used in evaluating the seed storage of nine genera of annual flowers. Relative humidity (RH) levels of 11%, 32%, 52%, and 75% provided wide ranges in seed moisture during storage at 5, 15, and 25C. At each temperature, total germination percentages (G) generally declined as seed moisture content increased during storage. The seed moisture range giving the highest G after 12 months of storage was determined for each temperature and plant genus. For all genera, seed moisture contents during storage increased as storage temperatures increased at constant RH levels. Moisture contents at 25C storage were 37%, 34%, 29%, and 20% higher than at 5C when RH levels were at 11%, 32%, 52%, and 75%, respectively.

scholarly journals Conservation of Garcinia imberti Bourd. through seeds

2019 ◽  
Vol 6 (2) ◽  
pp. 243-251
Author(s):  
M Anto ◽  
M Angala ◽  
P S Jothish ◽  
C Anilkumar
Keyword(s):  
Seed Germination ◽  
Relative Humidity ◽  
Moisture Content ◽  
Gibberellic Acid ◽  
Biosphere Reserve ◽  
Seed Storage ◽  
Seed Moisture Content ◽  
Evergreen Forests ◽  
Seed Moisture ◽  
Subsequent Exposure

Garcinia imberti seeds were collected during 2015-2017 from Shangili, Cheenikkala and Bonaccord evergreen forests of Agasthyamala Biosphere Reserve, the only abode of this endangered endemic species. Germinability of seeds were analysed through decoating, Gibberellic acid (GA3) and light inductive pre-treatments on fresh (62.8 % moisture content; MC) and desiccated (fast; 23.3% MC and slow; 30.5 % MC) seeds. The seed germination with impermeable coat (0.7-1.2 mm) was restricted which on decoating got enhanced. Application of GA3 along with exposure to light breaked dormancy within 4-6 days compared to non-treated seeds that took 238-254 days to germinate. Stored seeds behaviour revealed that seed moisture content and rate of germination were negatively correlated. Seed storage was found to be more efficient only up to 80 days at controlled seed banking conditions (20 ± 20C, 20 % relative humidity; RH). Both fast and slow desiccated seeds stored for 60 days in seed bank conditions exhibited 50.4 and 43.4 % of germination compared 39.4% germination of non-desiccated seeds. Hence fast desiccated and decoated G. imberti seeds pre-treated with GA3 on subsequent exposure to light alleviated dormancy. For seed banking, fast desiccated seeds with MC in between 40-20% are found to be promising.

scholarly journals Three-dimensional Models Represent Seed Moisture Content as a Function of Relative Humidity and Temperature

HortScience ◽  
1998 ◽  
Vol 33 (7) ◽  
pp. 1207-1209 ◽  
Author(s):  
Jian Fang ◽  
Frank Moore ◽  
Eric Roos ◽  
Christina Walters
Keyword(s):  
Relative Humidity ◽  
Moisture Content ◽  
Citrullus Lanatus ◽  
Three Dimensional ◽  
Selection Criterion ◽  
Seed Storage ◽  
Response Surfaces ◽  
Seed Moisture Content ◽  
Seed Moisture ◽  
Three Dimensional Models

Seed moisture content (MC) has been considered the most important factor controlling physiological reactions in seeds, and MC changes with relative humidity (RH) and temperature (T). This relationship is revealed by studying the interaction of RH and T at equilibrium. Cucumber (Cucumis sativus L.), lettuce (Lactuca sativa L.), maize (Zea mays L.), onion (Allium cepa L.), pea (Pisum sativum L.), and watermelon (Citrullus lanatus M. & N.) seeds were equilibrated over sulfuric acid (1% RH) and various saturated salt solutions (5.5% to 93% RH) at temperatures from 5 to 50 °C. Best-fit subset models were selected from the complete third-order model MC = β0 + β1*RH + β2*T + β3*RH2 + β4*T2 + β5*RH*T + β6*RH3 + β7*T3 + β8*RH*T2 + β9*RH2*T, using Mallows' minimum Cp as the selection criterion. All six best subset models (R2, 0.98 to 0.99) had the same functional form, MC = β0 + β1*RH + β2*T + β3*RH2 + β5*RH*T + β6*RH3 + β9*RH2*T. Coefficients had essentially the same respective values among all species except onion and pea, for which some coefficients were statistically different from those of the other species (P ≤ 0.05). All models indicated that seed MC increased as RH increased and decreased as T increased; but RH had the greater influence. The inverse relationship between seed MC and T, although slight, was evident in the response surfaces. The interaction effect of RH and T on MC was significant at P ≤0.001. These results suggest that orthodox seed species respond similarly to T and RH. This in turn suggests that a common model could be developed and used for optimizing seed storage environments.

EFFECT OF MOISTURE CONTENT ON VIABILITY AND INFESTATION OF HULLESS TERRA OATS IN STORAGE

1979 ◽  
Vol 59 (4) ◽  
pp. 911-916 ◽  
Author(s):  
R. N. SINHA ◽  
N. D. G. WHITE ◽  
H. A. H. WALLACE ◽  
R. I. H. McKENZIE
Keyword(s):  
Relative Humidity ◽  
Moisture Content ◽  
Mite Infestation ◽  
Tyrophagus Putrescentiae ◽  
Adsorption And Desorption ◽  
Safe Storage ◽  
Moisture Contents ◽  
Seed Moisture ◽  
Effect Of Moisture

The effects of various seed moisture contents in hulless (cv. Terra) and hulled oats (cv. Random) on susceptibility to mite infestation and on mycofloral growth and germination loss were studied at weekly intervals. Fat acidity values were determined for Terra oats only after 4 wk of storage. Moisture content-relative humidity adsorption and desorption curves were determined for Terra at 22 °C and at relative humidities of 35–100%. Terra oats, which had a higher level of Penicillium infection at 90–100% RH than Random oats, lost viability more rapidly than Random. Fat acidity values of Terra increased rapidly from 35 mg KOH/100 g of seed to 87–118 mg KOH/100 g of seed, only when seeds were stored at 90–100% RH. Terra offered a more favorable substrate for the multiplication of the mites Tyrophagus putrescentiae, Acarus farris, and Lepidoglyphus destructor than did Random. With the exception of susceptibility to mite infestation, safe storage criteria are similar for hulled and hulless oats at usual moisture contents.

Leek (Allium porrum L.) seed development and germination

1992 ◽  
Vol 2 (2) ◽  
pp. 89-95 ◽  
Author(s):  
D. Gray ◽  
J. R. A. Steckel ◽  
L. J. Hands
Keyword(s):  
Moisture Content ◽  
Seed Development ◽  
Developmental Stages ◽  
Dry Weight ◽  
Allium Porrum ◽  
Seed Moisture Content ◽  
Subsequent Performance ◽  
Moisture Contents ◽  
Seed Moisture ◽  
Seed Growth

AbstractThe effects of development of leek seeds at 20/10°, 25/15° and 30/20°C (day/night) and drying of seed harvested at different developmental stages on subsequent performance were examined in each of 3 years. An increase in temperature from 20/10° to 30/20°C reduced mean seed weight from 2.90 to 2.55 mg as a result of a reduction in the duration of seed growth from 80 to 55 days; seed growth rate was unaffected. Seed moisture content reached a minimum, up to 35 days after the attainment of maximum seed dry weight and 115, 90 and 70 days after anthesis at 20/10°, 25/15° and 30/20°C, respectively. The curves relating seed moisture to time for each temperature regime were mapped onto a single line accounting for >90% of the variation in moisture content, using accumulated day-degrees >6°C instead of chronological time. Seeds were capable of germinating when seed moisture contents were >60% (fresh weight basis), but maximum viability and minimum mean time to germination were not attained until seed moisture contents at harvest had fallen to 20–30%. Germination was little affected by temperature of seed development. Drying immature seeds increased percentage germination. Growing seeds at 30/20°C and drying at 35°C and 30% RH raised the upper temperature limit of germination compared with growing at 20/10°C and drying at 15°C and 30% RH.

Reimposition of conditional dormancy during air-dry storage of prechilled Sitka spruce seeds

1998 ◽  
Vol 8 (2) ◽  
pp. 113-122 ◽  
Author(s):  
Steve K. Jones ◽  
Peter G. Gosling ◽  
Richard H. Ellis
Keyword(s):  
Moisture Content ◽  
Water Potential ◽  
Seed Storage ◽  
Sitka Spruce ◽  
Picea Sitchensis ◽  
Seed Moisture Content ◽  
Moisture Contents ◽  
Dry Storage ◽  
Low Water Potential ◽  
Negative Exponential

AbstractPrechilling seeds of Sitka spruce (Picea sitchensis[Bong.] Carr.) at 4°C with 30% moisture content for 12–14 weeks (84–98 d) removed conditional dormancy (i.e. they were then able to germinate at 10°C). The non-dormant status was preserved after redrying to 6% moisture content. However, conditional dormancy was gradually reimposed during subsequent air-dry storage at 4°C and 6% seed moisture content in all five seed lots tested. Further investigations with one seed lot showed that reimposition was reversed by a second prechill treatment, but was reimposed again during subsequent air-dry storage. The trend of dormancy reimposition within seed lots over time was quantified by negative exponential relations between ability to germinate at 10°C and duration of air-dry storage. The progress of dormancy reimposition was influenced by seed storage moisture content and was most rapid at 4–10%. At higher moisture contents (15 and 20%) the rate of the reimposition of conditional dormancy was much reduced, while at moisture contents of 25 and 30% further loss in dormancy occurred. Thus it is clear that dormancy reimposition occurred during storage at low water potential rather than solely during desiccation from high to low water potential.

scholarly journals STORAGE OF TRUE POTATO SEED

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 577c-577
Author(s):  
Noël Pallais
Keyword(s):  
Moisture Content ◽  
Seed Dormancy ◽  
Potato Seed ◽  
True Potato Seed ◽  
Seedling Vigor ◽  
High Moisture ◽  
Seed Moisture Content ◽  
Moisture Contents ◽  
Seed Moisture

True potato seed of Atzimba × 104.12LB (intermediate dormancy) was dried to seed moisture contents ranging from 3.85 to 12.5% (dry wt basis) and was stored for 2 years at 30, 15 and 5°C. Seed was tested for various germination and seedling vigor criteria at 4 month intervals. Seed dormancy and viability were better preserved at seed moisture levels below 7% and as temperature decreased. High moisture (>9%) was lethal to seed stored at 30°C. TPS should be stored at <5% seed moisture content. Under this condition seed dormancy in the genotype studied was lost after about 12 months at 30°C.

scholarly journals Primed Lettuce Seeds Exhibit Increased Sensitivity to Moisture Content During Controlled Deterioration

HortScience ◽  
2007 ◽  
Vol 42 (6) ◽  
pp. 1436-1439 ◽  
Author(s):  
H.J. Hill ◽  
Jesse D. Cunningham ◽  
Kent J. Bradford ◽  
A.G. Taylor
Keyword(s):  
Moisture Content ◽  
Elevated Temperatures ◽  
Seed Viability ◽  
Seed Storage ◽  
Seed Priming ◽  
Storage Conditions ◽  
Seed Moisture Content ◽  
Moisture Contents ◽  
Lettuce Seeds ◽  
Rate Of Decline

The Ellis-Roberts seed viability equation is used to predict seed survival after storage at specified temperatures and moisture contents. Seed priming, which can break dormancy and accelerate germination, can also reduce seed storage life. Because primed seeds were not used in developing the Ellis-Roberts equation, the reciprocal nature of specific seed moisture content (MC, fresh weight basis) and temperatures that applies to nonprimed lettuce (Lactuca sativa L.) seeds may not apply to primed seeds. To determine how priming affects lettuce seeds in relation to the viability equation, an experiment was conducted using two cultivars, ‘Big Ben’ and ‘Parris Island Cos’. Seeds primed in polyethylene glycol 8000 (–1.45 MPa, 24 h at 15 °C) and nonprimed seeds were first adjusted to 6% and 9% moisture contents and then stored at 48 and 38 °C for up to 30 days, respectively. These storage conditions (6% MC and 48 °C; 9% MC and 38 °C) were predicted by the viability equation to result in equal longevities. Subsequent viability assays at 20 °C revealed that nonprimed seeds in both storage environments exhibited similar losses in viability over time, thus validating the Ellis-Roberts equation and the use of these conditions to apply different but equal aging stress. Primed seeds of both cultivars deteriorated faster than nonprimed seeds as expected. However, primed seeds did exhibit different rates of deterioration between the storage environments. Primed seeds stored at 9% MC and 38 °C deteriorated faster than primed seeds stored at 6% MC and 48 °C. The rate of decline in probit viability percentage was three times greater in primed ‘Big Ben’ seeds stored at 9% MC and 38 °C than for those stored at 6% MC and 48 °C (–1.34 versus –0.26 probits per day, respectively). ‘Parris Island Cos’ seeds stored at 9% MC and 38 °C had twice the rate of deterioration that those stored at 6% MC and 48 °C (–1.19 and –0.49 probits per day, respectively). The results indicate that primed lettuce seeds were more sensitive to the adverse effects of higher seed MC than were nonprimed seeds during storage at elevated temperatures.

scholarly journals Moisture Contents in Bottle Gourd Seeds during Dry Heat Treatment and Subsequent Germination

HortScience ◽  
2004 ◽  
Vol 39 (4) ◽  
pp. 758D-759
Author(s):  
Seung-Hee Lee* ◽  
Jin-Seok Kim ◽  
Jung-Myung Lee
Keyword(s):  
Heat Treatment ◽  
Moisture Content ◽  
Value Added ◽  
Bottle Gourd ◽  
Maximum Temperature ◽  
Seed Moisture Content ◽  
Dry Heat ◽  
Moisture Contents ◽  
Seed Moisture ◽  
Dry Heat Treatment

Dry heat treatment (DHT), a powerful and agrochemical-free means of inactivating seed-borne virus and other pathogens, has been extensively used for value-added vegetable seeds in Korea, Japan, and some other countries. Since seeds are treated with extremely high heat (75 °C or higher) for a long time (72 h or longer), heat-induced phytotoxicity symptoms are frequently observed. Even though various internal and external factors, such as seed maturity and vigor, maximum temperature and duration of DHT, are known to influence the severity of phytotoxicity, precise control of seed moisture contents during DHT is regarded as one of the most important factors for successful DHT. In an ideal condition using a specifically designed DTH machine, seed moisture content of bottle gourd, initially around 6.20% to 0.64% when stored in a storage room with 50% RH, decreased by 1% after 24 h at 35 °C (5.20% to 0.23%), and further decreased below 4% after 24 h pretreatment at 50 °C (3.64% to 0.37%). The seed moisture content was further reduced down to about 2% after 72 h DHT at 75 °C (2.16% to 0.28%). During the post-treatment conditioning at 50 °C and 70% RH for 24 h, the moisture contents were raised to about 6%(5.94% to 0.45%), thus approaching the initial moisture content of 6% to 7%. During the germination period, treated seeds showed slower absorption of water as compared to the intact seeds, thus suggesting that this slow absorption of initial moisture absorption may be responsible for the slow initial germination frequently observed in treated seeds. Final germination and seedling vigor were not affected by DHT.

scholarly journals Temperature and Relative Humidity Govern Germination and Storage of Gladiolus Seed

HortScience ◽  
1991 ◽  
Vol 26 (8) ◽  
pp. 1054-1057 ◽  
Author(s):  
W.J. Carpenter ◽  
G.J. Wilfret ◽  
J.A. Cornell
Keyword(s):  
Relative Humidity ◽  
Seed Treatment ◽  
No Reduction ◽  
Germination Rate ◽  
Seed Viability ◽  
Seed Storage ◽  
Moisture Contents ◽  
Seed Moisture ◽  
Germination Temperature ◽  
And Storage

Gladiolus (G. grandiflorus) seed germination was light-independent, but temperature influenced the germination rate. Constant 20C promoted higher total germination (97%), fewer days (4.3) to 50% of final germination, and shorter span of days (4.8) between 10% and 90% germination than other constant temperatures, although similar results were achieved by alternating 12-h cycles of 20 to 25C. Total germination was unchanged after seed treatment for 7 days at 10 to -20C, but longer germination periods were required after treatments below -10C. Reducing seed moisture contents from 11.8% to 4.2% caused no reduction in total germination, but moisture contents below 6.6% delayed achieving 50% of final germination and extended the periods from 10% to 90% of germination. Temperature and relative humidity (RH) during storage were important in retaining seed viability, with RH having a larger effect. Smallest declines in total germination during 12 months of storage occurred at 11% and 33% RH at 15C. The statistical analysis estimated the optimum seed storage at 14C and 26% RH.

scholarly journals Effect of Scarification, Seed Storage Temperature, and Relative Humidity on Lupinus havardii Wats. and Lupinus texensis Hook. Seed Germination

HortScience ◽  
2005 ◽  
Vol 40 (3) ◽  
pp. 782-785 ◽  
Author(s):  
Wayne A. Mackay
Keyword(s):  
Relative Humidity ◽  
Storage Temperature ◽  
Germination Rate ◽  
Seed Storage ◽  
Significant Decline ◽  
Rapid Decline ◽  
Seed Moisture Content ◽  
C Storage ◽  
Seed Moisture ◽  
Seed Scarification

Seeds of Lupinus havardii Wats. and L. texensis Hook. were subjected to scarification, storage temperature (4 or 22 °C), and relative humidity (RH) treatments (11%, 23%, 52%, 75%, or 97% RH) for 12 months. Seed moisture increased as relative humidity increased with scarified seed having the greatest increase in seed moisture content regardless of storage temperature. For both species, the combination of seed scarification before storage, 75% RH, and 22 °C storage temperature resulted in a significant and rapid decline in germinability beginning at 4 months. Scarified L. texensis seed stored at 52% RH and 22 °C also exhibited a significant decline in germinability following 6 months storage. Seed of both species stored under all other conditions germinated similar to or higher than the initial germination rate after 12 months. These results clearly show that scarification can be performed before seed packaging as long as the seed packets are stored at ≤23% RH under 4 or 22 °C with no loss in germinability for at least 1 year.

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